Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,686,256 A→G L166P (CTG→CCG)  b3530 ← putative oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,686,2560AG85.7% 675.9 / 97.5 266L166P (CTG→CCG) b3530putative oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base A (19/19);  new base G (117/111);  total (136/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

Only 100 of 274 total aligned reads displayed.

CCCGGCGTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGTACTCCAG  >  NC_000913/3686165‑3686348
                                                                                           |                                                                                            
 ccGGCGTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTCTGCggg                                                                                            >  2:429780/1‑93 (MQ=255)
    gCGTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGAGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGccc                                                                                         >  2:5224462/1‑93 (MQ=255)
      gTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGt                                                                                       <  1:3837493/93‑1 (MQ=255)
       ttCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGtt                                                                                      >  1:2998723/1‑92 (MQ=255)
       ttCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTa                                                                                      >  2:2571792/1‑93 (MQ=255)
       ttCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTa                                                                                      >  2:195534/1‑93 (MQ=255)
       ttCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTa                                                                                      <  1:2429953/93‑1 (MQ=255)
       ttCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTa                                                                                      >  2:3080218/1‑93 (MQ=255)
        tCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTAt                                                                                     <  1:5059791/93‑1 (MQ=255)
        tCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTAt                                                                                     <  2:3906051/93‑1 (MQ=255)
         cGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTAtt                                                                                    >  1:5836107/1‑93 (MQ=255)
            ttttGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGcc                                                                                 <  2:250971/93‑1 (MQ=255)
              ttGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCg                                                                               <  2:2445126/93‑1 (MQ=255)
                  cATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGc                                                                           >  2:3666182/1‑93 (MQ=255)
                  cATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGc                                                                           >  2:955577/1‑93 (MQ=255)
                    tCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCAt                                                                         <  2:6539631/93‑1 (MQ=255)
                    tCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGAGCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCAt                                                                         <  2:2684110/93‑1 (MQ=255)
                     cTGTTCCAGGACGGCAAAGCCTTCGTCACGGCGATCGCTACTAAACAGCAAGAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATc                                                                        <  1:4430707/93‑1 (MQ=255)
                     cTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGGGCATc                                                                        >  1:2115542/1‑93 (MQ=255)
                       gTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCg                                                                      >  1:1181013/1‑93 (MQ=255)
                        ttCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGc                                                                     <  1:5217550/93‑1 (MQ=255)
                         tCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCa                                                                    >  1:6154421/1‑93 (MQ=255)
                          ccAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGGAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCAt                                                                   <  2:5745075/93‑1 (MQ=255)
                          ccAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCAt                                                                   <  2:758695/93‑1 (MQ=255)
                          ccAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCAt                                                                   <  2:3851549/93‑1 (MQ=255)
                          ccAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCTCGTATTGCCCGGTGCATCCGCAt                                                                   >  2:2897642/1‑93 (MQ=255)
                           cAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCAtt                                                                  <  1:2267754/93‑1 (MQ=255)
                           cAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCAtt                                                                  <  2:1116797/93‑1 (MQ=255)
                             ggACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGa                                                                >  2:4139687/1‑93 (MQ=255)
                             ggACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGa                                                                >  1:2705851/1‑93 (MQ=255)
                             ggACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGa                                                                >  1:4720830/1‑93 (MQ=255)
                               aCGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATg                                                              >  1:2844062/1‑93 (MQ=255)
                               aCGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATg                                                              >  2:53725/1‑93 (MQ=255)
                                cGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGc                                                             >  1:1972122/1‑93 (MQ=255)
                                 ggCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCg                                                            <  1:669707/93‑1 (MQ=255)
                                 ggCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCg                                                            >  1:1835753/1‑93 (MQ=255)
                                 ggCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCg                                                            >  2:5004232/1‑93 (MQ=255)
                                  gCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGt                                                           <  2:5091711/93‑1 (MQ=255)
                                   cAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGtt                                                          <  2:5578866/93‑1 (MQ=255)
                                   cAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGtt                                                          >  2:2123294/1‑93 (MQ=255)
                                      aaCCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTa                                                       <  1:6122901/93‑1 (MQ=255)
                                      aaCCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTa                                                       <  1:2243245/93‑1 (MQ=255)
                                        ccTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAAc                                                     <  2:2226378/93‑1 (MQ=255)
                                        ccTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAAc                                                     <  2:5482477/93‑1 (MQ=255)
                                         cTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACt                                                    <  2:1103015/93‑1 (MQ=255)
                                            cGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGAt                                                 <  1:3680665/93‑1 (MQ=255)
                                            cGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGCGCATCCGCATTGATGCGTTTTAACTGAt                                                 >  1:3555199/1‑93 (MQ=255)
                                             gTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGAtt                                                >  1:4536258/1‑93 (MQ=255)
                                             gTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGAtt                                                <  1:1454476/93‑1 (MQ=255)
                                              tCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTa                                               <  2:2402593/93‑1 (MQ=255)
                                              tCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTa                                               >  2:1486300/1‑93 (MQ=255)
                                              tCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTa                                               <  1:4369337/93‑1 (MQ=255)
                                              tCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTa                                               >  2:3394060/1‑93 (MQ=255)
                                               cgcGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTaa                                              <  1:5624897/91‑1 (MQ=255)
                                               cACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTaa                                              >  2:1289199/1‑93 (MQ=255)
                                                aCGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAAt                                             <  1:416718/93‑1 (MQ=255)
                                                aCGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAAt                                             >  1:5956406/1‑93 (MQ=255)
                                                aCGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAAt                                             >  1:2551465/1‑93 (MQ=255)
                                                 cGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATc                                            <  1:4773713/93‑1 (MQ=255)
                                                 cGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATc                                            <  2:2343149/93‑1 (MQ=255)
                                                     gATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCtt                                        >  1:5111613/1‑93 (MQ=255)
                                                     gATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCtt                                        >  1:5433025/1‑93 (MQ=255)
                                                      aTCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTc                                       <  1:4272201/93‑1 (MQ=255)
                                                      aTCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTc                                       >  1:3401370/1‑93 (MQ=255)
                                                      aTCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTc                                       <  1:6390829/93‑1 (MQ=255)
                                                        cGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGc                                     >  2:4451531/1‑93 (MQ=255)
                                                        cGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGc                                     >  1:5760910/1‑93 (MQ=255)
                                                        cGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGc                                     >  1:4857690/1‑93 (MQ=255)
                                                         gCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGcc                                    <  2:6225061/93‑1 (MQ=255)
                                                           tactaAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCAc                                  >  1:6041156/1‑93 (MQ=255)
                                                           tactaAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCAc                                  >  1:4924645/1‑93 (MQ=255)
                                                           tactaAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCAc                                  <  1:4699441/93‑1 (MQ=255)
                                                           tactaAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAAACGCTTCGCCAc                                  <  1:5681684/93‑1 (MQ=255)
                                                              taAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGc                               >  1:1652643/1‑93 (MQ=255)
                                                               aaaCAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCg                              <  2:1693939/93‑1 (MQ=255)
                                                               aaaCAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCg                              <  2:1359665/93‑1 (MQ=255)
                                                                aaCAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGa                             <  2:5853867/93‑1 (MQ=255)
                                                                aaCAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGa                             >  2:4591156/1‑93 (MQ=255)
                                                                 aCAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAg                            >  2:1869253/1‑93 (MQ=255)
                                                                   aGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGcc                          <  2:5519266/93‑1 (MQ=255)
                                                                    gCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCg                         <  2:1417805/93‑1 (MQ=255)
                                                                      aaTAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGTCACGGCGAGCCGGa                       >  2:3592932/1‑93 (MQ=255)
                                                                       aTAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGaa                      >  2:6335194/1‑93 (MQ=255)
                                                                       aTAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGaa                      >  2:4723590/1‑93 (MQ=255)
                                                                        tAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAAt                     >  1:1039806/1‑93 (MQ=255)
                                                                        tAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAAt                     >  1:898239/1‑93 (MQ=255)
                                                                         aGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATAGCAGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCAGGAAtt                    <  2:6649657/93‑1 (MQ=255)
                                                                          gcgcCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAAttt                   <  2:3963061/93‑1 (MQ=255)
                                                                          gcgcCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAAttt                   >  1:2774635/1‑93 (MQ=255)
                                                                           cgcCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAAtttt                  <  1:1290739/93‑1 (MQ=255)
                                                                            gcCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGCATTTTc                 >  1:6433859/1‑93 (MQ=255)
                                                                              cAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGc               <  1:214489/93‑1 (MQ=255)
                                                                               aGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGcc              >  1:3624192/1‑93 (MQ=255)
                                                                               aGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGcc              >  1:5391490/1‑93 (MQ=255)
                                                                               aGATTGTTTTGCGGGCCCGTATTGCACGGTGCATGCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGAGAGCCGGCATTTTCGcc              >  1:6572889/1‑93 (MQ=255)
                                                                                 aTTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCAc            >  1:1996568/1‑93 (MQ=255)
                                                                                    gTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGt         <  2:5613124/93‑1 (MQ=255)
                                                                                    gTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGt         >  1:1475481/1‑93 (MQ=255)
                                                                                     ttttGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGTa        >  1:3091538/1‑93 (MQ=255)
                                                                                     ttttGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGTa        <  2:1193026/93‑1 (MQ=255)
                                                                                           |                                                                                            
CCCGGCGTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGTACTCCAG  >  NC_000913/3686165‑3686348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCCGGCGTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGTACTCCAGT  >  NC_000913/3686163‑3686349
                                                                                             |                                                                                             
CGCCCGGCGTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCG                                                                                               <  SRR4302547.1912346/94‑1 (MQ=60)
CGCCCGGCGTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCG                                                                                               <  SRR4302547.4973562/94‑1 (MQ=60)
 GCCCGGCGTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGG                                                                                              >  SRR4302547.4794483/1‑94 (MQ=60)
 GCCCGGCGTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAG                                                                                              >  SRR4302547.4601480/1‑94 (MQ=60)
      GCGTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTCTTGCAGGCCCA                                                                                         <  SRR4302547.3909530/94‑1 (MQ=60)
      GCGTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTAACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCG                                                                                         <  SRR4302547.2338452/94‑1 (MQ=60)
       CGTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGT                                                                                        <  SRR4302547.2714855/94‑1 (MQ=60)
        GTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTT                                                                                       >  SRR4302547.3058361/1‑94 (MQ=60)
        GTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTA                                                                                       <  SRR4302547.3915659/94‑1 (MQ=60)
        GTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTA                                                                                       >  SRR4302547.1208622/1‑94 (MQ=60)
        GTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTA                                                                                       >  SRR4302547.4473521/1‑94 (MQ=60)
        GTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTA                                                                                       <  SRR4302547.517280/94‑1 (MQ=60)
        GTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTA                                                                                       >  SRR4302547.6276075/1‑94 (MQ=60)
         TTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTAT                                                                                      <  SRR4302547.4223783/94‑1 (MQ=60)
         TTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTAT                                                                                      <  SRR4302547.2479115/94‑1 (MQ=60)
          TCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATT                                                                                     <  SRR4302547.5164213/94‑1 (MQ=60)
          TCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATT                                                                                     <  SRR4302547.4384250/94‑1 (MQ=60)
          TCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATT                                                                                     <  SRR4302547.1791470/94‑1 (MQ=60)
          TCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATT                                                                                     >  SRR4302547.5956853/1‑94 (MQ=60)
           CGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTG                                                                                    >  SRR4302547.2960007/1‑94 (MQ=60)
           CGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTG                                                                                    <  SRR4302547.3718506/94‑1 (MQ=60)
           CGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTG                                                                                    <  SRR4302547.5827760/94‑1 (MQ=60)
            GATTTTGCCATCTGTTCCAGGACCGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTTC                                                                                   >  SRR4302547.6151931/1‑94 (MQ=60)
             ATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCC                                                                                  <  SRR4302547.1484875/94‑1 (MQ=60)
              TTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCC                                                                                 >  SRR4302547.5485064/1‑94 (MQ=60)
               TTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCG                                                                                <  SRR4302547.689615/94‑1 (MQ=60)
                 TGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGT                                                                              <  SRR4302547.2845167/94‑1 (MQ=60)
                  GCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTG                                                                             >  SRR4302547.6181986/1‑94 (MQ=60)
                    CATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCA                                                                           <  SRR4302547.2608091/94‑1 (MQ=60)
                    CATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCA                                                                           >  SRR4302547.2174658/1‑94 (MQ=60)
                    CATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCA                                                                           <  SRR4302547.6339295/94‑1 (MQ=60)
                      TCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGGGCATC                                                                         >  SRR4302547.2157698/1‑94 (MQ=60)
                       CTGTTCCAGGACGGCAAAGCCTTCGTCACGGCGATCGCTACTAAACAGCAAGAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCC                                                                        <  SRR4302547.4522280/94‑1 (MQ=60)
                        TGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCG                                                                       <  SRR4302547.2012217/94‑1 (MQ=60)
                        TGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCG                                                                       >  SRR4302547.4483047/1‑94 (MQ=60)
                        TGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCG                                                                       <  SRR4302547.5234890/94‑1 (MQ=60)
                        TGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCG                                                                       >  SRR4302547.1204871/1‑94 (MQ=60)
                         GTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGC                                                                      >  SRR4302547.2119200/1‑94 (MQ=60)
                          TTCCAGGACGGCAAAACCTTCGTCACGGGGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCA                                                                     >  SRR4302547.4175424/1‑94 (MQ=60)
                          TTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCA                                                                     <  SRR4302547.5324943/94‑1 (MQ=60)
                          TTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCA                                                                     >  SRR4302547.6282123/1‑94 (MQ=60)
                           TCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCAT                                                                    >  SRR4302547.4922653/1‑94 (MQ=60)
                            TCAGGACGGCAAAACCTTCGTCAAGGCGGTCGCTACTAAACAACAATAGCGGCAGATTGCTTTGCGGGCCCGGATCGCCCGGTGCAgacttatt                                                                   >  SRR4302547.6443432/1‑86 (MQ=48)
                            CCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATT                                                                   >  SRR4302547.2485002/1‑94 (MQ=60)
                             CAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTG                                                                  <  SRR4302547.2313610/94‑1 (MQ=60)
                             CAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTG                                                                  <  SRR4302547.6029874/94‑1 (MQ=60)
                              AGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGA                                                                 >  SRR4302547.2760183/1‑94 (MQ=60)
                              AGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGA                                                                 >  SRR4302547.4818582/1‑94 (MQ=60)
                                GACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATG                                                               >  SRR4302547.2900886/1‑94 (MQ=60)
                                 ACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGC                                                              <  SRR4302547.3047721/94‑1 (MQ=60)
                                 ACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGC                                                              >  SRR4302547.2011283/1‑94 (MQ=60)
                                  CGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCG                                                             >  SRR4302547.1872043/1‑94 (MQ=60)
                                   GGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGT                                                            <  SRR4302547.682032/94‑1 (MQ=60)
                                     CAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTT                                                          >  SRR4302547.5868047/1‑94 (MQ=60)
                                     CAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTT                                                          >  SRR4302547.1473087/1‑94 (MQ=60)
                                      AAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTT                                                         >  SRR4302547.3140004/1‑94 (MQ=60)
                                      AAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTT                                                         >  SRR4302547.4630713/1‑94 (MQ=60)
                                       AAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTA                                                        <  SRR4302547.6594802/94‑1 (MQ=60)
                                        AACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAA                                                       <  SRR4302547.6249919/94‑1 (MQ=60)
                                        AACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAA                                                       <  SRR4302547.2288493/94‑1 (MQ=60)
                                           CTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTG                                                    >  SRR4302547.3636459/1‑94 (MQ=60)
                                           CTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTG                                                    <  SRR4302547.5213178/94‑1 (MQ=60)
                                           CTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTG                                                    >  SRR4302547.6765727/1‑94 (MQ=60)
                                            TTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGA                                                   <  SRR4302547.1795971/94‑1 (MQ=60)
                                             TCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGAT                                                  >  SRR4302547.5089546/1‑94 (MQ=60)
                                             TCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGCGCATCCGCATTGATGCGTTTTAACTGAT                                                  >  SRR4302547.3627752/1‑94 (MQ=60)
                                              CGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATT                                                 <  SRR4302547.3755809/94‑1 (MQ=60)
                                              CGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATT                                                 >  SRR4302547.4630008/1‑94 (MQ=60)
                                              CGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATT                                                 <  SRR4302547.1959305/94‑1 (MQ=60)
                                               GTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTA                                                <  SRR4302547.5786406/94‑1 (MQ=60)
                                               GTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTA                                                >  SRR4302547.1329028/1‑94 (MQ=60)
                                               GTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTA                                                <  SRR4302547.1483552/94‑1 (MQ=60)
                                                TCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAA                                               <  SRR4302547.4459100/94‑1 (MQ=60)
                                                TCACGCCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAA                                               <  SRR4302547.3091761/94‑1 (MQ=60)
                                                 CGCGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAAT                                              <  SRR4302547.5740971/94‑1 (MQ=60)
                                                 CACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAAT                                              >  SRR4302547.6583206/1‑94 (MQ=60)
                                                 CACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAAT                                              >  SRR4302547.2602989/1‑94 (MQ=60)
                                                 CACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAAT                                              >  SRR4302547.6079913/1‑94 (MQ=60)
                                                  ACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATC                                             <  SRR4302547.424445/94‑1 (MQ=60)
                                                  ACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATC                                             <  SRR4302547.365341/94‑1 (MQ=60)
                                                   CGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCG                                            <  SRR4302547.5690307/94‑1 (MQ=60)
                                                   CGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCG                                            <  SRR4302547.5888315/94‑1 (MQ=60)
                                                   CGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCG                                            <  SRR4302547.4872619/94‑1 (MQ=60)
                                                   CGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCG                                            <  SRR4302547.4876603/94‑1 (MQ=60)
                                                      CGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTCACTGATTAATCGCTT                                         >  SRR4302547.1940398/1‑94 (MQ=60)
                                                      CGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTT                                         >  SRR4302547.5545334/1‑94 (MQ=60)
                                                      CGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTT                                         >  SRR4302547.5216951/1‑94 (MQ=60)
                                                       GATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTC                                        >  SRR4302547.3470588/1‑94 (MQ=60)
                                                        ATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCG                                       <  SRR4302547.1575338/94‑1 (MQ=60)
                                                        ATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCG                                       <  SRR4302547.4359895/94‑1 (MQ=60)
                                                        ATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCG                                       <  SRR4302547.6524173/94‑1 (MQ=60)
                                                        ATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCG                                       <  SRR4302547.4964529/94‑1 (MQ=60)
                                                         TCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGC                                      >  SRR4302547.5880051/1‑94 (MQ=60)
                                                         TCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGC                                      <  SRR4302547.4858716/94‑1 (MQ=60)
                                                         TCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGC                                      >  SRR4302547.4958331/1‑94 (MQ=60)
                                                          CGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCC                                     >  SRR4302547.6136276/1‑94 (MQ=60)
                                                          CGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCC                                     >  SRR4302547.2342949/1‑94 (MQ=60)
                                                          CGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCC                                     >  SRR4302547.4643100/1‑94 (MQ=60)
                                                          CGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCC                                     >  SRR4302547.480110/1‑94 (MQ=60)
                                                           GCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCA                                    <  SRR4302547.1245851/94‑1 (MQ=60)
                                                           GCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCA                                    >  SRR4302547.277316/1‑94 (MQ=60)
                                                           GCTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCA                                    >  SRR4302547.2867438/1‑94 (MQ=60)
                                                           GCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCA                                    <  SRR4302547.4823277/94‑1 (MQ=60)
                                                            CTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCAC                                   >  SRR4302547.6166511/1‑94 (MQ=60)
                                                            CTACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCAC                                   >  SRR4302547.5026654/1‑94 (MQ=60)
                                                             TACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACG                                  <  SRR4302547.4796778/94‑1 (MQ=60)
                                                             TACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACG                                  <  SRR4302547.4350848/94‑1 (MQ=60)
                                                             TACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAAACGCTTCGCCACG                                  <  SRR4302547.5798995/94‑1 (MQ=60)
                                                             TACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACG                                  <  SRR4302547.3724575/94‑1 (MQ=60)
                                                              ACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGG                                 >  SRR4302547.6467004/1‑94 (MQ=60)
                                                              ACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGG                                 >  SRR4302547.6542791/1‑94 (MQ=60)
                                                              ACTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGG                                 >  SRR4302547.6534067/1‑94 (MQ=60)
                                                              ACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTAGCCACGG                                 >  SRR4302547.2536125/1‑94 (MQ=60)
                                                               CTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGC                                >  SRR4302547.1685249/1‑94 (MQ=60)
                                                               CTAAACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGC                                >  SRR4302547.5755821/1‑94 (MQ=60)
                                                                  AACAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAG                             <  SRR4302547.5245735/94‑1 (MQ=60)
                                                                   ACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGC                            >  SRR4302547.2278966/1‑94 (MQ=60)
                                                                    CAGCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCC                           <  SRR4302547.4983315/94‑1 (MQ=60)
                                                                      GCAATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGG                         <  SRR4302547.690033/94‑1 (MQ=60)
                                                                        AATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAA                       >  SRR4302547.5523374/1‑94 (MQ=60)
                                                                         ATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAAT                      >  SRR4302547.915508/1‑94 (MQ=60)
                                                                         ATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAAT                      >  SRR4302547.1060300/1‑94 (MQ=60)
                                                                         ATAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAAT                      >  SRR4302547.3320167/1‑94 (MQ=60)
                                                                          TAGCGCCAGTTTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATT                     <  SRR4302547.4571954/94‑1 (MQ=60)
                                                                          TAGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATT                     <  SRR4302547.1144575/94‑1 (MQ=60)
                                                                           AGCGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTT                    >  SRR4302547.2830212/1‑94 (MQ=60)
                                                                             CGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGCATTTTC                  >  SRR4302547.6567955/1‑94 (MQ=60)
                                                                             CGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTC                  <  SRR4302547.1316723/94‑1 (MQ=60)
                                                                             CGCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTC                  >  SRR4302547.3498886/1‑94 (MQ=60)
                                                                              GCCAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCG                 >  SRR4302547.211514/1‑94 (MQ=60)
                                                                                CAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCC               >  SRR4302547.5502840/1‑94 (MQ=60)
                                                                                CAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCC               >  SRR4302547.5427833/1‑94 (MQ=60)
                                                                                CAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCC               >  SRR4302547.3698118/1‑94 (MQ=60)
                                                                                CAGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCC               <  SRR4302547.218540/94‑1 (MQ=60)
                                                                                CAGATTGTTTTGCGGGCCCGTATTGCACGGTGCATGCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGAGAGCCGGCATTTTCGCC               >  SRR4302547.6709733/1‑94 (MQ=60)
                                                                                CAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCC               <  SRR4302547.5624778/94‑1 (MQ=60)
                                                                                 AGATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCA              >  SRR4302547.2310293/1‑94 (MQ=60)
                                                                                  GATTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCAC             >  SRR4302547.2036287/1‑94 (MQ=60)
                                                                                    TTGTTTTGCGGGCCGGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCG           <  SRR4302547.4281324/94‑1 (MQ=60)
                                                                                    TTGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCG           <  SRR4302547.6063915/94‑1 (MQ=60)
                                                                                     TGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGT          >  SRR4302547.2243517/1‑94 (MQ=60)
                                                                                     TGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGT          >  SRR4302547.2184263/1‑94 (MQ=60)
                                                                                     TGTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGT          >  SRR4302547.1504955/1‑94 (MQ=60)
                                                                                      GTTTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGTA         >  SRR4302547.3152840/1‑94 (MQ=60)
                                                                                        TTTGCGGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGTACT       <  SRR4302547.1563185/94‑1 (MQ=60)
                                                                                            CAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGTACTCCAG   >  SRR4302547.3589049/1‑94 (MQ=60)
                                                                                             GGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGTACTCCAGT  >  SRR4302547.4674485/1‑94 (MQ=60)
                                                                                             GGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGTACTCCAGA  <  SRR4302547.5933661/94‑1 (MQ=60)
                                                                                             AGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGTACTCCAGT  <  SRR4302547.6238554/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGCCCGGCGTTCGATTTTGCCATCTGTTCCAGGACGGCAAAACCTTCGTCACGGCGATCGCTACTAAACAGCAATAGCGCCAGATTGTTTTGCAGGCCCGTATTGCCCGGTGCATCCGCATTGATGCGTTTTAACTGATTAATCGCTTCGCCACGGCGAGCCGGAATTTTCGCCACCGTACTCCAGT  >  NC_000913/3686163‑3686349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑