Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,190	(C)_6→7	coding (948/1725 nt)	yhjW ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,184	1	.	C	84.0%	681.3 / 123.3	225	coding (954/1725 nt)	yhjW	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (24/12); new base C (96/93); total (120/105)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01

Only 100 of 255 total aligned reads displayed.

TGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGC  >  NC_000913/3707092‑3707276

 gCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCg‑cg‑c                                                                                             <  2:308961/93‑1 (MQ=255)

 gCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCg‑cg‑c                                                                                             <  2:5377043/93‑1 (MQ=255)

   aTAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccc                                                                                           >  1:6290984/1‑93 (MQ=255)

   aTAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccc                                                                                           >  1:1428716/1‑93 (MQ=255)

   aTAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccc                                                                                           >  2:5868129/1‑93 (MQ=255)

   aTAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccc                                                                                           >  1:2371280/1‑93 (MQ=255)

      gCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑cccccc                                                                                        <  1:447560/93‑1 (MQ=255)

      gCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑cccccc                                                                                        <  2:4768880/93‑1 (MQ=255)

         tAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccccccctg                                                                                     >  1:4551203/1‑90 (MQ=255)

         tAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccccccctg                                                                                     >  2:2768599/1‑90 (MQ=255)

         tAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccccccctg                                                                                     <  1:3933192/93‑4 (MQ=255)

         tAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccccccctc                                                                                     >  2:4283944/1‑90 (MQ=255)

          agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGTGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGAc                                                                                    >  1:3690943/1‑93 (MQ=255)

          agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccccccctga                                                                                    <  1:2055531/93‑5 (MQ=255)

          agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccccccctga                                                                                    <  2:1571146/93‑5 (MQ=255)

             ggTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGt                                                                                  <  2:481664/93‑1 (MQ=255)

              gTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTa                                                                                 >  2:1680343/1‑93 (MQ=255)

               tCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTAc                                                                                <  2:1962088/93‑1 (MQ=255)

                cAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATTTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACa                                                                               >  2:128643/1‑93 (MQ=255)

                cAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACa                                                                               >  2:5588069/1‑93 (MQ=255)

                   aaCTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAAc                                                                            <  1:5719068/93‑1 (MQ=255)

                    aCTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACa                                                                           <  1:4116264/93‑1 (MQ=255)

                     cTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACAt                                                                          <  2:4025183/93‑1 (MQ=255)

                     cTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACAt                                                                          <  1:5326543/93‑1 (MQ=255)

                       gAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGc                                                                        <  1:5413820/93‑1 (MQ=255)

                       gAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGc                                                                        >  1:5285142/1‑93 (MQ=255)

                        aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCa                                                                       <  2:5093730/93‑1 (MQ=255)

                          tCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAAc                                                                     <  1:5944102/93‑1 (MQ=255)

                          tCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAAc                                                                     <  2:2125536/93‑1 (MQ=255)

                           ccTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACg                                                                    >  2:5699040/1‑93 (MQ=255)

                            cTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGc                                                                   <  2:113897/93‑1 (MQ=255)

                             tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAg                                                                 >  1:1941664/1‑93 (MQ=255)

                             tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAg                                                                 >  2:5928405/1‑93 (MQ=255)

                              aaCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAg                                                                 <  1:1542264/93‑1 (MQ=255)

                              aaCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAg                                                                 <  1:925877/93‑1 (MQ=255)

                               aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGt                                                                >  1:2291323/1‑93 (MQ=255)

                               aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGt                                                                <  2:3758957/93‑1 (MQ=255)

                               aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTg                                                               >  2:511916/1‑93 (MQ=255)

                                cTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTg                                                               <  2:28450/93‑1 (MQ=255)

                                    ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGagag                                                           >  2:780931/1‑93 (MQ=255)

                                    ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGt                                                          >  2:4629725/1‑93 (MQ=255)

                                     tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAACCATGCAACGCAGTGAGAGtt                                                         <  2:6222767/93‑1 (MQ=255)

                                      cAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGtt                                                         >  1:5462825/1‑93 (MQ=255)

                                       aGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGttt                                                        >  2:2402173/1‑93 (MQ=255)

                                        gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTg                                                       >  1:1404600/1‑93 (MQ=255)

                                        gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTg                                                       >  1:3281562/1‑93 (MQ=255)

                                        gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTg                                                       <  2:4588507/93‑1 (MQ=255)

                                          aCCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                     <  2:6208085/93‑1 (MQ=255)

                                            cgcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTg                                                   >  1:6415625/1‑93 (MQ=255)

                                            cgcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTg                                                   <  2:6158666/93‑1 (MQ=255)

                                             gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc                                                  <  2:5051835/93‑1 (MQ=255)

                                               gAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                <  2:380429/93‑1 (MQ=255)

                                               gAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                >  1:3979819/1‑93 (MQ=255)

                                               gAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                <  2:6570817/93‑1 (MQ=255)

                                                   aTGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca                                           >  2:5016470/1‑93 (MQ=255)

                                                    tgttctgttcTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca                                           <  2:2003775/93‑1 (MQ=255)

                                                    tgttctgttcTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca                                           >  2:5533992/1‑93 (MQ=255)

                                                      ttctgttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcaca                                         <  2:3928020/93‑1 (MQ=255)

                                                      ttctgttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcaca                                         >  2:3400350/1‑93 (MQ=255)

                                                       tctgttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAg                                        >  1:2559638/1‑93 (MQ=255)

                                                        ctgttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAgg                                       <  2:2276395/93‑1 (MQ=255)

                                                        ctgttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAgg                                       <  2:5162208/93‑1 (MQ=255)

                                                        ctgttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGCGAGAGTTTGGTTGCGGTATCACAgg                                       >  2:6548361/1‑93 (MQ=255)

                                                         tgttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGTTTGCGGTATCACAGGa                                      >  1:3911326/1‑93 (MQ=255)

                                                         tgttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGa                                      >  2:6414775/1‑93 (MQ=255)

                                                         tgttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGa                                      <  2:1876577/93‑1 (MQ=255)

                                                         tgttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGa                                      <  2:3144591/93‑1 (MQ=255)

                                                          gttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg                                     >  1:3125136/1‑93 (MQ=255)

                                                           ttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGt                                    >  1:1235949/1‑93 (MQ=255)

                                                            tctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTa                                   >  1:2977449/1‑93 (MQ=255)

                                                              ttttAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAc                                 <  1:1710497/93‑1 (MQ=255)

                                                              ttttAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAc                                 >  1:3459543/1‑93 (MQ=255)

                                                               tttAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc                                >  1:5647344/1‑93 (MQ=255)

                                                                ttAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCa                               <  1:1663767/93‑1 (MQ=255)

                                                                 tAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCAc                              <  1:3516827/93‑1 (MQ=255)

                                                                 tAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCAc                              <  1:1499867/93‑1 (MQ=255)

                                                                 tAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACg                             <  1:2551967/93‑1 (MQ=255)

                                                                  aaTGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACg                             >  2:318396/1‑93 (MQ=255)

                                                                   aTGTGCGCTGCGGATTATCTTCCGCCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACgg                            <  1:2792363/93‑1 (MQ=255)

                                                                   aTGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGa                           >  2:1757704/1‑93 (MQ=255)

                                                                      tgCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAc                         <  2:2037799/93‑1 (MQ=255)

                                                                          cTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAcgccg                     >  2:6147951/1‑93 (MQ=255)

                                                                            gCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCa                  >  1:1089248/1‑93 (MQ=255)

                                                                               gATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGa                <  1:1920307/93‑1 (MQ=255)

                                                                               gATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGa                <  2:6341587/93‑1 (MQ=255)

                                                                               gATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGa                >  2:3495734/1‑93 (MQ=255)

                                                                               gATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGa                >  1:720121/1‑93 (MQ=255)

                                                                               gATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGa                >  2:4407632/1‑93 (MQ=255)

                                                                                aTTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAt               >  2:257984/1‑93 (MQ=255)

                                                                                aTTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAt               >  2:2952021/1‑93 (MQ=255)

                                                                                   aTCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAtttt            >  1:4794422/1‑93 (MQ=255)

                                                                                    tCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAttttt           <  2:4201616/93‑1 (MQ=255)

                                                                                     cTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTtc          <  2:1384727/93‑1 (MQ=255)

                                                                                     cTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTtc          <  2:1898739/93‑1 (MQ=255)

                                                                                      ttCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTtct         >  1:6385448/1‑93 (MQ=255)

                                                                                      ttCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGATTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTtct         >  1:6083118/1‑93 (MQ=255)

                                                                                       tCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTtctc        <  1:5194148/93‑1 (MQ=255)

                                                                                        ccG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTTGTTGCGGTATCACAGGAGTAAACACGGAACGCCGCCAGACTTTTCTcc       >  1:4197077/1‑93 (MQ=255)

                                                                                        ccG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCt      >  2:2995488/1‑93 (MQ=255)

                                                                                             g‑cCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGc  >  2:5779671/1‑93 (MQ=255)

TGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGC  >  NC_000913/3707092‑3707276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTC  >  NC_000913/3707098‑3707283

GCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCT                                                                                              <  SRR4302547.2784628/94‑1 (MQ=60)

GCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCC                                                                                              <  SRR4302547.6235884/94‑1 (MQ=60)

GCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCC                                                                                              <  SRR4302547.3620304/94‑1 (MQ=60)

GCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCC                                                                                              <  SRR4302547.455882/94‑1 (MQ=60)

 CGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCct                                                                                             <  SRR4302547.3385441/94‑3 (MQ=60)

  GTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCctg                                                                                            >  SRR4302547.4645338/1‑91 (MQ=60)

   TAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGTGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGAC                                                                                           >  SRR4302547.3766295/1‑94 (MQ=60)

   TAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGA                                                                                            >  SRR4302547.4572839/1‑94 (MQ=60)

   TAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGA                                                                                            >  SRR4302547.5375880/1‑94 (MQ=60)

   TAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGA                                                                                            <  SRR4302547.4013188/94‑1 (MQ=60)

    AGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGAC                                                                                           <  SRR4302547.1445360/94‑1 (MQ=60)

    AGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGAC                                                                                           <  SRR4302547.2096535/94‑1 (MQ=60)

       GGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCCTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTA                                                                                        >  SRR4302547.2809185/1‑94 (MQ=60)

         TCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAA                                                                                     >  SRR4302547.711279/1‑94 (MQ=60)

            GAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACA                                                                                  <  SRR4302547.5395130/94‑1 (MQ=60)

             AACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACA                                                                                  <  SRR4302547.5837203/94‑1 (MQ=60)

             AACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACA                                                                                  <  SRR4302547.2245067/94‑1 (MQ=60)

              ACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACAT                                                                                 <  SRR4302547.4199920/94‑1 (MQ=60)

               CTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATG                                                                                >  SRR4302547.1945109/1‑94 (MQ=60)

               CTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATG                                                                                <  SRR4302547.5436328/94‑1 (MQ=60)

                TGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGC                                                                               >  SRR4302547.5394155/1‑94 (MQ=60)

                 GAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCA                                                                              <  SRR4302547.5525797/94‑1 (MQ=60)

                  AATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAA                                                                             <  SRR4302547.4641418/94‑1 (MQ=60)

                  AATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAA                                                                             >  SRR4302547.4155128/1‑94 (MQ=60)

                  AATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAA                                                                             <  SRR4302547.5315421/94‑1 (MQ=60)

                   ATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAAC                                                                            >  SRR4302547.3551185/1‑94 (MQ=60)

                    TCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACG                                                                           <  SRR4302547.6067364/94‑1 (MQ=60)

                    TCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACG                                                                           <  SRR4302547.6375781/94‑1 (MQ=60)

                    TCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACG                                                                           >  SRR4302547.1003752/1‑94 (MQ=60)

                     CCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCA                                                                         >  SRR4302547.1207441/1‑94 (MQ=60)

                      CTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAG                                                                        >  SRR4302547.1980167/1‑94 (MQ=60)

                       TAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAG                                                                        >  SRR4302547.5883586/1‑94 (MQ=60)

                       TAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAG                                                                        <  SRR4302547.2599221/94‑1 (MQ=60)

                        AACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGT                                                                       <  SRR4302547.943760/94‑1 (MQ=60)

                        AACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGT                                                                       >  SRR4302547.2337739/1‑94 (MQ=60)

                        AACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGT                                                                       <  SRR4302547.1572963/94‑1 (MQ=60)

                         ACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTG                                                                      >  SRR4302547.4701384/1‑94 (MQ=60)

                           TGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGA                                                                   <  SRR4302547.1607474/94‑1 (MQ=60)

                             CTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAG                                                                  >  SRR4302547.6083617/1‑94 (MQ=60)

                             CTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGT                                                                 <  SRR4302547.211218/94‑1 (MQ=60)

                              TTCAGAACCGCGAATATGTTCTGTTCTGTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAGCGCAGTGAGAGTT                                                                <  SRR4302547.4814281/94‑1 (MQ=60)

                               TCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTT                                                                <  SRR4302547.2463045/94‑1 (MQ=60)

                               TCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTT                                                                >  SRR4302547.5575672/1‑94 (MQ=60)

                                CAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTT                                                               >  SRR4302547.3100803/1‑94 (MQ=60)

                                CAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTG                                                              >  SRR4302547.334592/1‑94 (MQ=60)

                                 AGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTG                                                              >  SRR4302547.3347352/1‑94 (MQ=60)

                                 AGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTG                                                              >  SRR4302547.2605942/1‑94 (MQ=60)

                                 AGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTG                                                              >  SRR4302547.1432701/1‑94 (MQ=60)

                                  GAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGG                                                             <  SRR4302547.3998008/94‑1 (MQ=60)

                                  GAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGG                                                             <  SRR4302547.5713282/94‑1 (MQ=60)

                                  GAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGG                                                             >  SRR4302547.2365890/1‑94 (MQ=60)

                                   AACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGT                                                            >  SRR4302547.5067626/1‑94 (MQ=60)

                                    ACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTT                                                           <  SRR4302547.1983175/94‑1 (MQ=60)

                                     CCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTG                                                          >  SRR4302547.6549405/1‑94 (MQ=60)

                                       GCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCG                                                        >  SRR4302547.1546717/1‑94 (MQ=60)

                                        CGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGG                                                       >  SRR4302547.4060743/1‑94 (MQ=60)

                                         GAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGT                                                      >  SRR4302547.1432233/1‑94 (MQ=60)

                                         GAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGT                                                      >  SRR4302547.1651569/1‑94 (MQ=60)

                                         GAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGT                                                      >  SRR4302547.1741633/1‑94 (MQ=60)

                                          AAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTA                                                     <  SRR4302547.5533318/94‑1 (MQ=60)

                                           AAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTAT                                                    <  SRR4302547.5742572/94‑1 (MQ=60)

                                           AAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATC                                                   >  SRR4302547.1403700/1‑94 (MQ=60)

                                             ATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCA                                                  >  SRR4302547.642626/1‑94 (MQ=60)

                                                TTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAG                                               >  SRR4302547.2611331/1‑94 (MQ=60)

                                                TTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAG                                               <  SRR4302547.6542747/94‑1 (MQ=60)

                                                TTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAG                                               >  SRR4302547.1746786/1‑94 (MQ=60)

                                                 TCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGG                                              >  SRR4302547.2044684/1‑94 (MQ=60)

                                                 TCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGG                                              >  SRR4302547.426986/1‑94 (MQ=60)

                                                  CTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGTTTGCGGTATCACAGGA                                             >  SRR4302547.3990863/1‑94 (MQ=60)

                                                  CTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGA                                             >  SRR4302547.6776297/1‑94 (MQ=60)

                                                  CTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGA                                             >  SRR4302547.1001268/1‑94 (MQ=60)

                                                   TGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAG                                            >  SRR4302547.3187131/1‑94 (MQ=60)

                                                    GTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGT                                           <  SRR4302547.421750/94‑1 (MQ=60)

                                                    GTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGT                                           >  SRR4302547.1260886/1‑94 (MQ=60)

                                                     TTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTA                                          >  SRR4302547.3036687/1‑94 (MQ=60)

                                                       CTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAC                                        >  SRR4302547.4333154/1‑94 (MQ=60)

                                                       CTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAC                                        >  SRR4302547.3585968/1‑94 (MQ=60)

                                                       CTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAC                                        >  SRR4302547.3530078/1‑94 (MQ=60)

                                                        TTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACC                                       >  SRR4302547.5763908/1‑94 (MQ=60)

                                                        TTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACC                                       <  SRR4302547.1744124/94‑1 (MQ=60)

                                                          TTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCAC                                     <  SRR4302547.1696566/94‑1 (MQ=60)

                                                           TAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACG                                    <  SRR4302547.1529784/94‑1 (MQ=60)

                                                           TAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACG                                    <  SRR4302547.3588617/94‑1 (MQ=60)

                                                           TAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTGTGGTTGCTGTATCACAGGAGTAACCACG                                    <  SRR4302547.651273/94‑1 (MQ=60)

                                                           TAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGG                                   <  SRR4302547.2603498/94‑1 (MQ=60)

                                                            AATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAACACTG                                   >  SRR4302547.1517830/1‑94 (MQ=60)

                                                             ATGTGCGCTGCGGATTATCTTCCGCCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGA                                  <  SRR4302547.2848280/94‑1 (MQ=60)

                                                             ATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGA                                  <  SRR4302547.1973676/94‑1 (MQ=60)

                                                              TGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAA                                 <  SRR4302547.1474632/94‑1 (MQ=60)

                                                               GTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAC                                <  SRR4302547.5271814/94‑1 (MQ=60)

                                                               GTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAC                                <  SRR4302547.5769933/94‑1 (MQ=60)

                                                               GTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAC                                >  SRR4302547.1844169/1‑94 (MQ=60)

                                                               GTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACG                               <  SRR4302547.5898009/94‑1 (MQ=60)

                                                                TGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGCACGC                              >  SRR4302547.3923342/1‑94 (MQ=60)

                                                                 GCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGC                              >  SRR4302547.3098685/1‑94 (MQ=60)

                                                                  CGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCC                             <  SRR4302547.6512127/94‑1 (MQ=60)

                                                                  CGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCC                             >  SRR4302547.5491994/1‑94 (MQ=60)

                                                                  CGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCC                             <  SRR4302547.3224203/94‑1 (MQ=60)

                                                                    CTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGC                           <  SRR4302547.4019881/94‑1 (MQ=60)

                                                                     TGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCA                         >  SRR4302547.1111056/1‑94 (MQ=60)

                                                                        GGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA                       >  SRR4302547.794827/1‑94 (MQ=60)

                                                                        GGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA                       >  SRR4302547.733516/1‑94 (MQ=60)

                                                                         GATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAT                      <  SRR4302547.1958340/94‑1 (MQ=60)

                                                                            TATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTT                   >  SRR4302547.4893768/1‑94 (MQ=60)

                                                                            TATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTT                   <  SRR4302547.3945321/94‑1 (MQ=60)

                                                                               CTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCT                >  SRR4302547.6518694/1‑94 (MQ=60)

                                                                               CTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGATTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCT                >  SRR4302547.6209321/1‑94 (MQ=60)

                                                                                TTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTC               >  SRR4302547.550231/1‑94 (MQ=60)

                                                                                 TCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTTGTTGCGGTATCACAGGAGTAAACACGGAACGCCGCCAGACTTTTCTCC              >  SRR4302547.4282669/1‑94 (MQ=60)

                                                                                 TCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCC              <  SRR4302547.5301047/94‑1 (MQ=60)

                                                                                  CCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCT             >  SRR4302547.3697373/1‑94 (MQ=60)

                                                                                   CG‑C‑‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTACTGGG          <  SRR4302547.4892249/94‑1 (MQ=60)

                                                                                    G‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGG           <  SRR4302547.2165950/94‑1 (MQ=60)

                                                                                    G‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGG           <  SRR4302547.1110212/94‑1 (MQ=60)

                                                                                    G‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGG           <  SRR4302547.4516107/94‑1 (MQ=60)

                                                                                    G‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGG           <  SRR4302547.2122388/94‑1 (MQ=60)

                                                                                       gcCCCCCCTGACGTACACACATGCAACGCAGCGAGAGCTTGGATGCGGTATCACAGGAGTAAGCACGGAACGCCGCCAGATTTCTCTCCTGGGC         >  SRR4302547.3296699/3‑94 (MQ=60)

                                                                                       gcCCCCCCTGACGTACAAAAATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGCACGCAGCCAGATTTTTCTCCTGGGC         >  SRR4302547.43936/3‑94 (MQ=60)

                                                                                         CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCA       <  SRR4302547.838384/94‑1 (MQ=60)

                                                                                          CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAG      >  SRR4302547.2120985/1‑94 (MQ=60)

                                                                                          CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAG      >  SRR4302547.5943008/1‑94 (MQ=60)

                                                                                          CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAG      <  SRR4302547.3044209/94‑1 (MQ=60)

                                                                                          CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAG      >  SRR4302547.5700598/1‑94 (MQ=60)

                                                                                            CCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTT    >  SRR4302547.6301000/1‑94 (MQ=60)

                                                                                             CCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTT   <  SRR4302547.2170822/94‑1 (MQ=60)

                                                                                             CCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTT   >  SRR4302547.526148/1‑94 (MQ=60)

                                                                                             CCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTT   >  SRR4302547.3084965/1‑94 (MQ=60)

                                                                                             CCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTT   >  SRR4302547.2221504/1‑94 (MQ=60)

                                                                                              CTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTC  <  SRR4302547.6151963/94‑1 (MQ=60)

GCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTC  >  NC_000913/3707098‑3707283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`