Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,759,803 A→G intergenic (‑221/‑7) yibF ← / → rhsA putative S‑transferase/RhsA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,759,8030AG85.0% 683.4 / 100.7 267intergenic (‑221/‑7)yibF/rhsAputative S‑transferase/RhsA
Reads supporting (aligned to +/- strand):  ref base A (23/17);  new base G (124/103);  total (147/120)
Fisher's exact test for biased strand distribution p-value = 8.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

Only 100 of 272 total aligned reads displayed.

TTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGG  >  NC_000913/3759711‑3759895
                                                                                            |                                                                                            
ttattTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATATCCCGATAAAACAAGGa                                                                                              <  1:5344264/93‑1 (MQ=255)
   tttCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTga                                                                                           <  2:3290478/93‑1 (MQ=255)
   tttCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATga                                                                                           <  2:6522915/93‑1 (MQ=255)
     tCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTgaga                                                                                         >  2:1401172/1‑93 (MQ=255)
        ctctGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAAt                                                                                      >  2:887496/1‑93 (MQ=255)
        ctctGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAAt                                                                                      >  1:5107207/1‑93 (MQ=255)
        ctctGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAAt                                                                                      >  2:2318879/1‑93 (MQ=255)
        ctctGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAAt                                                                                      >  2:3609624/1‑93 (MQ=255)
        ctctGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAAt                                                                                      >  1:1725058/1‑93 (MQ=255)
         tctGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAAGGTGAGAAAta                                                                                     >  1:3080348/1‑92 (MQ=255)
         tatGATGGATATCTCTCTTCAGGCTTTCTTATCAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATg                                                                                     <  2:2203254/91‑1 (MQ=14)
          ctGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGa                                                                                    >  1:4294072/1‑93 (MQ=255)
          ctGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGa                                                                                    <  1:3886475/93‑1 (MQ=255)
            gATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGc                                                                                  >  2:1466529/1‑93 (MQ=255)
             aTGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCg                                                                                 <  2:5917977/93‑1 (MQ=255)
             aTGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCg                                                                                 >  2:1917004/1‑93 (MQ=255)
             aTGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCg                                                                                 <  1:2329371/93‑1 (MQ=255)
               ggATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGa                                                                               <  2:3523446/93‑1 (MQ=255)
               ggATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGa                                                                               <  2:6107973/93‑1 (MQ=255)
                gATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGaa                                                                              <  2:1821830/93‑1 (MQ=255)
                 atatCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGaaa                                                                             <  1:1794792/93‑1 (MQ=255)
                 atatCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGaaa                                                                             >  2:6595745/1‑93 (MQ=255)
                 atatCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGGGAGAAATGAGCGGaaa                                                                             >  2:1544084/1‑93 (MQ=255)
                  tatCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGaaaa                                                                            <  1:5668685/93‑1 (MQ=255)
                   atCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAAc                                                                           >  1:3303994/1‑93 (MQ=255)
                    tctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAAcc                                                                          >  2:1631169/1‑93 (MQ=255)
                    tctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAAcc                                                                          >  2:5282628/1‑93 (MQ=255)
                     ctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCg                                                                         <  1:2165035/93‑1 (MQ=255)
                       cACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATACCCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGc                                                                       <  1:1089285/93‑1 (MQ=255)
                       cACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGc                                                                       <  2:4619866/93‑1 (MQ=255)
                         cTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAg                                                                     >  1:2795376/1‑93 (MQ=255)
                         cTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAg                                                                     >  2:2397827/1‑93 (MQ=255)
                           tCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAgcg                                                                   >  1:2471725/1‑93 (MQ=255)
                            cAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAgcgc                                                                  >  1:2104623/1‑93 (MQ=255)
                             aGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGcgcg                                                                 >  2:4336186/1‑93 (MQ=255)
                             aGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGcgcg                                                                 <  2:5011604/93‑1 (MQ=255)
                              ggCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGt                                                                <  1:1202167/93‑1 (MQ=255)
                               gCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTc                                                               <  1:4112367/93‑1 (MQ=255)
                               gCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTc                                                               <  1:5963708/93‑1 (MQ=255)
                               gCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTc                                                               >  2:5372257/1‑93 (MQ=255)
                                cTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCa                                                              <  1:4035440/93‑1 (MQ=255)
                                cTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCa                                                              >  2:3028155/1‑93 (MQ=255)
                                cTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCa                                                              >  2:111434/1‑93 (MQ=255)
                                 tttCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAg                                                             >  2:4269831/1‑93 (MQ=255)
                                  ttCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAgg                                                            <  2:6240859/93‑1 (MQ=255)
                                  ttCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAgg                                                            <  1:4626105/93‑1 (MQ=255)
                                   tCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAggg                                                           <  1:1516551/93‑1 (MQ=255)
                                      tataAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGa                                                        <  1:4997581/93‑1 (MQ=255)
                                        taAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACa                                                      <  2:3976153/93‑1 (MQ=255)
                                           agcTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGa                                                   <  1:2896571/91‑1 (MQ=255)
                                            tCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGAc                                                  >  2:6045833/1‑93 (MQ=255)
                                             cTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACg                                                 <  1:45232/93‑1 (MQ=255)
                                               gTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCa                                               >  2:1050015/1‑93 (MQ=255)
                                                tAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAg                                              >  1:1369414/1‑93 (MQ=255)
                                                gaGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAg                                              <  1:867360/92‑1 (MQ=255)
                                                 aGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGt                                             <  2:2963783/93‑1 (MQ=255)
                                                   ggTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTAt                                           <  2:4452577/93‑1 (MQ=255)
                                                     tttCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATgg                                         <  1:4887701/93‑1 (MQ=255)
                                                     tttCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATgg                                         <  2:705291/93‑1 (MQ=255)
                                                       tCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCg                                       >  1:6616946/1‑93 (MQ=255)
                                                         gCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCggg                                     >  2:335947/1‑92 (MQ=255)
                                                         gCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGt                                     <  1:1825194/93‑1 (MQ=255)
                                                         gCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGt                                     >  1:5288453/1‑93 (MQ=255)
                                                         gCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGt                                     >  1:2573154/1‑93 (MQ=255)
                                                          ccTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTa                                    >  1:4339049/1‑93 (MQ=255)
                                                           cTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAg                                   <  2:4810146/93‑1 (MQ=255)
                                                           cTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAg                                   <  1:2758721/93‑1 (MQ=255)
                                                           cTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAg                                   >  2:2677344/1‑93 (MQ=255)
                                                            tGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGc                                  <  1:5871176/93‑1 (MQ=255)
                                                             gTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCa                                 <  2:4552918/93‑1 (MQ=255)
                                                              tCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCAt                                <  1:1277491/93‑1 (MQ=255)
                                                               cagcagACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCAtt                               >  2:4198027/1‑93 (MQ=255)
                                                                agcagACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTg                              >  1:708958/1‑93 (MQ=255)
                                                                agcagACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTg                              >  2:4982071/1‑93 (MQ=255)
                                                                   agACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTc                           >  2:5766408/1‑93 (MQ=255)
                                                                   agACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTc                           <  2:5090281/93‑1 (MQ=255)
                                                                    gACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCa                          <  1:6515536/93‑1 (MQ=255)
                                                                     aCAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAg                         <  2:2589711/93‑1 (MQ=255)
                                                                     aCAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAg                         >  1:5165857/1‑93 (MQ=255)
                                                                     aCAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAg                         >  1:4173474/1‑93 (MQ=255)
                                                                      cAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAgg                        >  1:55185/1‑93 (MQ=255)
                                                                       aaaTAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAggg                       >  1:1063340/1‑93 (MQ=255)
                                                                        aaTAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGt                      <  1:3713178/93‑1 (MQ=255)
                                                                        aaTAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGt                      >  1:4813885/1‑93 (MQ=255)
                                                                         aTAACCCGATAAAACAAGGGCGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGtt                     >  2:2635856/1‑93 (MQ=255)
                                                                          tAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTc                    <  2:5257443/93‑1 (MQ=255)
                                                                            aCCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAg                  >  1:320378/1‑93 (MQ=255)
                                                                            aCCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAg                  <  1:526836/93‑1 (MQ=255)
                                                                            aCCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAg                  <  1:2552675/93‑1 (MQ=255)
                                                                               cGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCg               >  2:2431931/1‑93 (MQ=255)
                                                                                gATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCgg              >  1:195177/1‑93 (MQ=255)
                                                                                 aTAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCggg             <  2:365042/93‑1 (MQ=255)
                                                                                 aTAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCggg             >  1:6257686/1‑93 (MQ=255)
                                                                                   aaaaCAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGt           >  2:68860/1‑93 (MQ=255)
                                                                                   aaaaCAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGt           >  2:314647/1‑93 (MQ=255)
                                                                                     aaCAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTgc         >  2:5616812/1‑93 (MQ=255)
                                                                                     aaCAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTgc         >  2:377408/1‑93 (MQ=255)
                                                                                     aaCAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTgc         >  2:78724/1‑93 (MQ=255)
                                                                                        aaGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCa      <  1:2516807/93‑1 (MQ=255)
                                                                                         aGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCAt     >  1:4932040/1‑93 (MQ=255)
                                                                                            |                                                                                            
TTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGG  >  NC_000913/3759711‑3759895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGT  >  NC_000913/3759710‑3759896
                                                                                             |                                                                                             
ATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGG                                                                                               >  SRR4302547.3650870/1‑94 (MQ=57)
 TTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATATCCCGATAAAACAAGGAT                                                                                              <  SRR4302547.5454435/94‑1 (MQ=53)
 TTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGT                                                                                              <  SRR4302547.4110927/94‑1 (MQ=54)
  TATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAGCCCGATAAAACAAGGGTG                                                                                             <  SRR4302547.2711039/94‑1 (MQ=49)
  TATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTG                                                                                             <  SRR4302547.4567000/94‑1 (MQ=51)
   ATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGA                                                                                            >  SRR4302547.1266697/1‑94 (MQ=48)
     TTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGA                                                                                          <  SRR4302547.6722302/94‑1 (MQ=45)
     TTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGA                                                                                          >  SRR4302547.2618986/1‑94 (MQ=45)
     TTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGA                                                                                          >  SRR4302547.2208565/1‑94 (MQ=45)
     TTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGA                                                                                          >  SRR4302547.4644243/1‑94 (MQ=45)
      TCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAA                                                                                         >  SRR4302547.5379489/1‑94 (MQ=45)
      TCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAA                                                                                         >  SRR4302547.3286738/1‑94 (MQ=47)
        ACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAAT                                                                                       >  SRR4302547.3884237/1‑94 (MQ=45)
        ACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAAT                                                                                       >  SRR4302547.5212462/1‑94 (MQ=45)
        ACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAAT                                                                                       >  SRR4302547.1758964/1‑94 (MQ=45)
         CTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATG                                                                                      <  SRR4302547.2538172/94‑1 (MQ=45)
         CTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAAGGTGAGAAATA                                                                                      >  SRR4302547.3141517/1‑94 (MQ=33)
          TCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGA                                                                                     >  SRR4302547.4382340/1‑94 (MQ=45)
          TCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGA                                                                                     <  SRR4302547.1176085/94‑1 (MQ=45)
           CTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAG                                                                                    <  SRR4302547.3965530/94‑1 (MQ=45)
           CTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAG                                                                                    <  SRR4302547.5627437/94‑1 (MQ=45)
            TGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGC                                                                                   <  SRR4302547.1159365/94‑1 (MQ=45)
            TGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGC                                                                                   >  SRR4302547.2930217/1‑94 (MQ=45)
            TGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGC                                                                                   <  SRR4302547.4797435/94‑1 (MQ=47)
             GATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCG                                                                                  <  SRR4302547.4533486/94‑1 (MQ=45)
              ATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGG                                                                                 <  SRR4302547.2376590/94‑1 (MQ=47)
                GGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAA                                                                               <  SRR4302547.1758458/94‑1 (MQ=45)
                  ATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAA                                                                             <  SRR4302547.1830309/94‑1 (MQ=43)
                  ATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAA                                                                             >  SRR4302547.5264449/1‑94 (MQ=43)
                   TATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAAC                                                                            >  SRR4302547.3370536/1‑94 (MQ=43)
                   TATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAAC                                                                            <  SRR4302547.5785732/94‑1 (MQ=43)
                    ATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACC                                                                           <  SRR4302547.4416567/94‑1 (MQ=43)
                      CTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGG                                                                         <  SRR4302547.2208335/94‑1 (MQ=43)
                      CTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGG                                                                         <  SRR4302547.5088200/94‑1 (MQ=43)
                        CACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATACCCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCA                                                                       <  SRR4302547.1111094/94‑1 (MQ=43)
                         ACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAG                                                                      >  SRR4302547.3124126/1‑94 (MQ=48)
                         ACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAG                                                                      <  SRR4302547.5657030/94‑1 (MQ=48)
                         ACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAG                                                                      >  SRR4302547.2851332/1‑94 (MQ=48)
                         ACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAG                                                                      >  SRR4302547.1087168/1‑94 (MQ=48)
                         ACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAG                                                                      >  SRR4302547.4654391/1‑94 (MQ=50)
                          CTTCAGGGTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGC                                                                     >  SRR4302547.3631302/1‑94 (MQ=43)
                          CTTCAGGCTTTCTTATAAATCTGTATGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGTCAGC                                                                     <  SRR4302547.2452869/94‑1 (MQ=37)
                          CTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGC                                                                     <  SRR4302547.1900728/94‑1 (MQ=48)
                          CTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACAGGCAGC                                                                     >  SRR4302547.2609065/1‑94 (MQ=43)
                           TTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCG                                                                    <  SRR4302547.4097099/94‑1 (MQ=48)
                           TTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCG                                                                    >  SRR4302547.2521687/1‑94 (MQ=48)
                            TCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGC                                                                   >  SRR4302547.2146563/1‑94 (MQ=48)
                             CAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCG                                                                  >  SRR4302547.3151056/1‑94 (MQ=45)
                             CAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCG                                                                  <  SRR4302547.459044/94‑1 (MQ=45)
                             CAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCG                                                                  >  SRR4302547.4311820/1‑94 (MQ=45)
                              AGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGT                                                                 >  SRR4302547.2129265/1‑94 (MQ=43)
                              AGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAAAAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGT                                                                 <  SRR4302547.1497777/94‑1 (MQ=43)
                              AGGCTTTCTTATAAATCTGTAGGGATTCCCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGTGCGGAAAACCGGCAGCGCGT                                                                 >  SRR4302547.2488904/1‑94 (MQ=39)
                               GGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTC                                                                <  SRR4302547.1226429/94‑1 (MQ=43)
                                GCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCA                                                               <  SRR4302547.4195946/94‑1 (MQ=43)
                                GCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCA                                                               <  SRR4302547.6087389/94‑1 (MQ=43)
                                 CTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAG                                                              <  SRR4302547.4117372/94‑1 (MQ=43)
                                 CTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAG                                                              >  SRR4302547.6034554/1‑94 (MQ=43)
                                 CTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAG                                                              >  SRR4302547.496129/1‑94 (MQ=43)
                                  TTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGG                                                             <  SRR4302547.695874/94‑1 (MQ=43)
                                   TTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGG                                                            <  SRR4302547.4721917/94‑1 (MQ=43)
                                    TCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGC                                                           <  SRR4302547.1546764/94‑1 (MQ=43)
                                     CTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCG                                                          >  SRR4302547.2232699/1‑94 (MQ=43)
                                     CTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCG                                                          <  SRR4302547.6543246/94‑1 (MQ=43)
                                     CTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCG                                                          <  SRR4302547.5499082/94‑1 (MQ=43)
                                       TATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGAC                                                        <  SRR4302547.5100918/94‑1 (MQ=43)
                                        ATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACA                                                       <  SRR4302547.3876760/94‑1 (MQ=43)
                                         TAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACAT                                                      >  SRR4302547.913/1‑94 (MQ=43)
                                            ATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGAC                                                   >  SRR4302547.4201987/1‑94 (MQ=43)
                                            AGCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGAC                                                   <  SRR4302547.2954335/94‑1 (MQ=44)
                                              CTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGC                                                 >  SRR4302547.2663844/1‑94 (MQ=43)
                                              CTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGC                                                 <  SRR4302547.46070/94‑1 (MQ=45)
                                               TGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCA                                                >  SRR4302547.1260682/1‑94 (MQ=43)
                                               TGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCA                                                >  SRR4302547.2999651/1‑94 (MQ=43)
                                               TGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCA                                                <  SRR4302547.1424235/94‑1 (MQ=45)
                                                GTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAG                                               >  SRR4302547.1396894/1‑94 (MQ=43)
                                                 TAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGT                                              >  SRR4302547.5030239/1‑94 (MQ=43)
                                                 GAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGT                                              <  SRR4302547.883961/94‑1 (MQ=42)
                                                   GGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGGAT                                            <  SRR4302547.4272836/94‑1 (MQ=42)
                                                     GTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGG                                          >  SRR4302547.1648947/1‑94 (MQ=43)
                                                     GTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGCGCGGCATGACGCAGTATGG                                          >  SRR4302547.4897934/1‑94 (MQ=39)
                                                      TTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGC                                         <  SRR4302547.4988951/94‑1 (MQ=43)
                                                      TTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGC                                         <  SRR4302547.6601247/94‑1 (MQ=45)
                                                       TTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCG                                        >  SRR4302547.6754668/1‑94 (MQ=43)
                                                       TTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCG                                        >  SRR4302547.6260040/1‑94 (MQ=43)
                                                       TTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCG                                        >  SRR4302547.1828578/1‑94 (MQ=45)
                                                        TCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGG                                       <  SRR4302547.5310308/94‑1 (MQ=43)
                                                         CGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGT                                      >  SRR4302547.5397549/1‑94 (MQ=43)
                                                         CGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGT                                      >  SRR4302547.2625103/1‑94 (MQ=45)
                                                          GCCTGTCAGCAGACAAATACCCCGATAAAACAAGCGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTA                                     <  SRR4302547.6661859/94‑1 (MQ=39)
                                                          GCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTA                                     <  SRR4302547.1861277/94‑1 (MQ=43)
                                                          GCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTA                                     >  SRR4302547.4428290/1‑94 (MQ=43)
                                                          GCCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTA                                     >  SRR4302547.2538164/1‑94 (MQ=43)
                                                          GCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTA                                     >  SRR4302547.705273/1‑94 (MQ=45)
                                                           CCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAG                                    >  SRR4302547.2602499/1‑94 (MQ=43)
                                                           CCTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAG                                    >  SRR4302547.2164908/1‑94 (MQ=43)
                                                            CTGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGC                                   <  SRR4302547.2814016/94‑1 (MQ=43)
                                                             TGTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCA                                  <  SRR4302547.6203809/94‑1 (MQ=43)
                                                             TGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCA                                  <  SRR4302547.5992703/94‑1 (MQ=45)
                                                             TGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCA                                  <  SRR4302547.3156139/94‑1 (MQ=45)
                                                              GTCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCAT                                 >  SRR4302547.5383550/1‑94 (MQ=43)
                                                               TCAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATT                                <  SRR4302547.1303219/94‑1 (MQ=43)
                                                                CAGCAGACAAATAACCCGATAAAACAAGGGTTAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTG                               >  SRR4302547.1792550/1‑94 (MQ=41)
                                                                CAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTG                               >  SRR4302547.5715922/1‑94 (MQ=43)
                                                                CAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTG                               >  SRR4302547.2929780/1‑94 (MQ=43)
                                                                CAGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTG                               >  SRR4302547.722070/1‑94 (MQ=43)
                                                                CAGCAGACAAATAACCCGATAAAACAAGGGAGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGAAGCAGGATGGCGGTAGCATTG                               >  SRR4302547.805267/1‑94 (MQ=37)
                                                                 AGCGGACAAATAACCCGATAAAACAAGGGTGAGAAATTAGCGGAAAACCGGCAGTGCGTCAGGGCGACATGACGCAGTGTGGCGGGAGCAGTGT                              >  SRR4302547.3327268/1‑94 (MQ=33)
                                                                 AGCAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGT                              <  SRR4302547.4126565/94‑1 (MQ=43)
                                                                   CAGACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTC                            <  SRR4302547.1369150/94‑1 (MQ=48)
                                                                     GACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAG                          >  SRR4302547.5272214/1‑94 (MQ=53)
                                                                     GACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAG                          <  SRR4302547.6651182/94‑1 (MQ=53)
                                                                     GACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAG                          >  SRR4302547.4258491/1‑94 (MQ=55)
                                                                      ACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGG                         >  SRR4302547.5199102/1‑94 (MQ=55)
                                                                      ACAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGG                         <  SRR4302547.501971/94‑1 (MQ=55)
                                                                      ACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGG                         >  SRR4302547.56221/1‑94 (MQ=58)
                                                                       CAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGG                        >  SRR4302547.1084501/1‑94 (MQ=55)
                                                                       CAAATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGG                        >  SRR4302547.2645510/1‑94 (MQ=55)
                                                                        AAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGT                       >  SRR4302547.4913653/1‑94 (MQ=58)
                                                                         AATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTT                      <  SRR4302547.2827645/94‑1 (MQ=55)
                                                                         AATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTT                      >  SRR4302547.984775/1‑94 (MQ=55)
                                                                         AATAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTT                      <  SRR4302547.3788976/94‑1 (MQ=55)
                                                                          ATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTC                     >  SRR4302547.3999473/1‑94 (MQ=58)
                                                                           TAACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCA                    >  SRR4302547.5885485/1‑94 (MQ=55)
                                                                            AACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAG                   <  SRR4302547.3699404/94‑1 (MQ=55)
                                                                            AACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAG                   >  SRR4302547.79197/1‑94 (MQ=55)
                                                                            AACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAG                   >  SRR4302547.326337/1‑94 (MQ=55)
                                                                             ACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGC                  <  SRR4302547.2604223/94‑1 (MQ=55)
                                                                             ACCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGC                  <  SRR4302547.536362/94‑1 (MQ=55)
                                                                              CCCGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCC                 <  SRR4302547.5048066/94‑1 (MQ=55)
                                                                               CCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCG                >  SRR4302547.6081151/1‑94 (MQ=58)
                                                                                CGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGG               >  SRR4302547.5352323/1‑94 (MQ=55)
                                                                                CGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGG               >  SRR4302547.198826/1‑94 (MQ=55)
                                                                                CGATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGG               >  SRR4302547.5436279/1‑94 (MQ=55)
                                                                                 GATAAAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGG              >  SRR4302547.6387674/1‑94 (MQ=55)
                                                                                     AAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGC          >  SRR4302547.5242463/1‑94 (MQ=55)
                                                                                     AAACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGC          >  SRR4302547.4251405/1‑94 (MQ=55)
                                                                                      AACAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCG         <  SRR4302547.1849847/94‑1 (MQ=55)
                                                                                        CAAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCA       <  SRR4302547.1994149/94‑1 (MQ=55)
                                                                                         AAGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCAT      <  SRR4302547.2567685/94‑1 (MQ=55)
                                                                                         AAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCAT      >  SRR4302547.5034192/1‑94 (MQ=60)
                                                                                          AGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATT     <  SRR4302547.1969418/94‑1 (MQ=58)
                                                                                          AGGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATT     >  SRR4302547.260762/1‑94 (MQ=58)
                                                                                           GGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTG    >  SRR4302547.4233321/1‑94 (MQ=60)
                                                                                           GGGTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTG    <  SRR4302547.5949853/94‑1 (MQ=60)
                                                                                             GTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGT  <  SRR4302547.2316888/94‑1 (MQ=60)
                                                                                             GTGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGT  >  SRR4302547.5691619/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGT  >  NC_000913/3759710‑3759896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑