Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,824,873 G→A L679L (CTG→CTA)  recG → DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,824,8730GA87.9% 824.9 / 75.4 272L679L (CTG→CTA) recGDNA helicase
Reads supporting (aligned to +/- strand):  ref base G (15/18);  new base A (126/113);  total (141/131)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

Only 100 of 278 total aligned reads displayed.

GGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCTTC  >  NC_000913/3824783‑3824962
                                                                                          |                                                                                         
 gATTTACTGCGCGATCAGGCGATGATCCGGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATa                                                                                        <  2:3363641/93‑1 (MQ=255)
 gATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATa                                                                                        >  1:4244940/1‑93 (MQ=255)
 gATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATa                                                                                        <  1:3520525/93‑1 (MQ=255)
  aTTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAg                                                                                       <  1:1825413/93‑1 (MQ=255)
  aTTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAg                                                                                       <  1:2273326/93‑1 (MQ=255)
  aTTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAg                                                                                       >  2:2857540/1‑93 (MQ=255)
   tttACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGa                                                                                      >  1:3838116/1‑93 (MQ=255)
   tttACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGa                                                                                      >  2:5023079/1‑93 (MQ=255)
    ttACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGaa                                                                                     <  2:6294052/93‑1 (MQ=255)
     tACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAAc                                                                                    <  1:2511884/93‑1 (MQ=255)
     tACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAAc                                                                                    <  2:2618328/93‑1 (MQ=255)
     tACTGCGCGATCAGGCGATGATCCCGGAAGCTCAGCGCCTGGCACCCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAAc                                                                                    <  1:1734206/93‑1 (MQ=255)
         gcgcGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTg                                                                                <  1:6401537/93‑1 (MQ=255)
          cgcgATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTgg                                                                               <  2:4010073/93‑1 (MQ=255)
          cgcgATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTgg                                                                               <  2:2730855/93‑1 (MQ=255)
           gcgATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGa                                                                              >  1:5795391/1‑93 (MQ=255)
           gcgATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGa                                                                              <  2:4182006/93‑1 (MQ=255)
           gcgATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGa                                                                              <  2:4371455/93‑1 (MQ=255)
            cgATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGAt                                                                             <  2:4491349/93‑1 (MQ=255)
            cgATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGAt                                                                             >  1:4268473/1‑93 (MQ=255)
               tCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGcc                                                                          <  1:3091312/93‑1 (MQ=255)
                cAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCg                                                                         >  2:4563910/1‑93 (MQ=255)
                  ggCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGga                                                                       <  2:3661256/93‑1 (MQ=255)
                  ggCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGga                                                                       >  1:1047236/1‑93 (MQ=255)
                  ggCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGga                                                                       >  1:4569383/1‑93 (MQ=255)
                    cGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGgaga                                                                     <  2:2761114/93‑1 (MQ=255)
                    cGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGgaga                                                                     <  2:2084259/93‑1 (MQ=255)
                    cGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGgaga                                                                     <  1:5030798/93‑1 (MQ=255)
                     gatgatCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGAc                                                                    <  2:600521/93‑1 (MQ=255)
                     gatgatCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGAc                                                                    >  2:1856091/1‑93 (MQ=255)
                      atgatCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACg                                                                   >  2:881790/1‑93 (MQ=255)
                      atgatCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACg                                                                   >  1:917415/1‑93 (MQ=255)
                       tgatCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACgg                                                                  >  2:1270419/1‑93 (MQ=255)
                        gatCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGa                                                                 <  2:5584091/93‑1 (MQ=255)
                        gatCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGa                                                                 >  2:4685617/1‑93 (MQ=255)
                          tCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAAc                                                               <  2:6206088/93‑1 (MQ=255)
                          tCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAAc                                                               <  2:305660/93‑1 (MQ=255)
                          tCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAAc                                                               <  1:1509405/93‑1 (MQ=255)
                            ccGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGt                                                             >  1:2600642/1‑93 (MQ=255)
                            ccGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGt                                                             >  1:3969524/1‑93 (MQ=255)
                              ggAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTa                                                           >  1:1546715/1‑93 (MQ=255)
                              ggAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTa                                                           >  2:4420091/1‑93 (MQ=255)
                                aaGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACt                                                         <  2:4275259/93‑1 (MQ=255)
                                 aGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTc                                                        >  2:4108226/1‑93 (MQ=255)
                                 aGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTc                                                        >  1:1308567/1‑93 (MQ=255)
                                 aGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTc                                                        >  1:4853979/1‑93 (MQ=255)
                                 aGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTc                                                        <  1:321893/93‑1 (MQ=255)
                                     cAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAAt                                                    >  2:6630110/1‑93 (MQ=255)
                                       gcgcCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGc                                                  >  1:5914377/1‑93 (MQ=255)
                                       gcgcCTGGCACGACATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATgg                                                  >  1:1583718/1‑92 (MQ=255)
                                         gcCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGt                                                >  1:6193024/1‑93 (MQ=255)
                                          ccTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTa                                               <  2:248786/93‑1 (MQ=255)
                                            tGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTaaa                                             >  2:3896602/1‑93 (MQ=255)
                                             ggCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTaaaa                                            <  1:5139522/93‑1 (MQ=255)
                                              ccACGCCATATTCACGAACGTAACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACTTTACTCGAATGCGTAAAAg                                           <  1:2927430/92‑1 (MQ=255)
                                               cACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAgg                                          >  2:3636778/1‑93 (MQ=255)
                                               cACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAgg                                          <  2:6474309/93‑1 (MQ=255)
                                                 cGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAggcg                                        <  2:1109339/93‑1 (MQ=255)
                                                 cGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAggcg                                        <  2:4915549/93‑1 (MQ=255)
                                                  gCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAggcgg                                       <  2:1957434/93‑1 (MQ=255)
                                                  gCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAggcgg                                       >  2:6099578/1‑93 (MQ=255)
                                                   ccATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAggcggc                                      >  1:2140842/1‑93 (MQ=255)
                                                   ccATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAggcggc                                      <  2:2925321/93‑1 (MQ=255)
                                                   ccATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAggcggc                                      >  1:5653860/1‑93 (MQ=255)
                                                    cATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCa                                     <  1:894260/93‑1 (MQ=255)
                                                     atatTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAg                                    <  1:208857/93‑1 (MQ=255)
                                                     atatTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAg                                    <  1:6458919/93‑1 (MQ=255)
                                                       atTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTg                                  <  1:5266246/93‑1 (MQ=255)
                                                        ttCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTga                                 <  2:3713814/93‑1 (MQ=255)
                                                        ttCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTga                                 >  1:3670478/1‑93 (MQ=255)
                                                         tCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTgag                                >  2:4232144/1‑93 (MQ=255)
                                                          cACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTgaga                               <  2:5426170/93‑1 (MQ=255)
                                                              aaCGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGAc                           >  1:4177946/1‑93 (MQ=255)
                                                              aaCGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGAc                           >  1:3399424/1‑93 (MQ=255)
                                                               aCGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGAcc                          <  2:5826024/93‑1 (MQ=255)
                                                                 gTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGc                        <  2:3290243/93‑1 (MQ=255)
                                                                   tACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCa                      >  2:5246185/1‑93 (MQ=255)
                                                                    aCCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCAt                     >  2:3826120/1‑93 (MQ=255)
                                                                     cccACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCAtt                    >  2:2977741/1‑93 (MQ=255)
                                                                     cccACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCAtt                    <  2:1726530/93‑1 (MQ=255)
                                                                       cacaACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTc                  <  1:6646798/93‑1 (MQ=255)
                                                                         caacaGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAg                >  2:1092828/1‑93 (MQ=255)
                                                                          aacaGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAgg               >  2:6561830/1‑93 (MQ=255)
                                                                           acaGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGt              <  2:1404342/93‑1 (MQ=255)
                                                                           acaGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGt              <  1:3637208/93‑1 (MQ=255)
                                                                           acaGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGt              >  1:1673321/1‑93 (MQ=255)
                                                                           acaGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGt              <  1:124108/93‑1 (MQ=255)
                                                                            ccagCAAAATCCCCAATAAGACGGAGGCTGCCCGAGACGGAACGTTAACCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGtt             <  1:2564178/90‑1 (MQ=255)
                                                                             aGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTa            >  1:5218533/1‑93 (MQ=255)
                                                                              ggCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTAc           >  1:548931/1‑93 (MQ=255)
                                                                                 aaaaGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCt        <  2:3544893/93‑1 (MQ=255)
                                                                                   aaGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTAc      <  2:5269803/93‑1 (MQ=255)
                                                                                   aaGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTAc      >  2:5681333/1‑93 (MQ=255)
                                                                                   aaGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTAc      <  1:5383277/93‑1 (MQ=255)
                                                                                    aGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTAcc     <  1:2425557/93‑1 (MQ=255)
                                                                                     gCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCt    >  2:2391693/1‑93 (MQ=255)
                                                                                     gCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCt    <  2:1024705/93‑1 (MQ=255)
                                                                                     gCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCt    <  1:3310699/93‑1 (MQ=255)
                                                                                      cccTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCtt   >  2:1479312/1‑93 (MQ=255)
                                                                                       ccTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCTTc  >  2:3463454/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCTTC  >  NC_000913/3824783‑3824962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCTTCCTGC  >  NC_000913/3824780‑3824966
                                                                                             |                                                                                             
GGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTG                                                                                               <  SRR4302547.2387560/94‑1 (MQ=60)
 GCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAA                                                                                              <  SRR4302547.6008986/94‑1 (MQ=60)
 GCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAA                                                                                              >  SRR4302547.4276171/1‑94 (MQ=60)
  CGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAAT                                                                                             <  SRR4302547.684687/94‑1 (MQ=60)
  CGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAAT                                                                                             <  SRR4302547.3895132/94‑1 (MQ=60)
  CGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAAT                                                                                             >  SRR4302547.4096217/1‑94 (MQ=60)
  CGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAAT                                                                                             <  SRR4302547.6240337/94‑1 (MQ=60)
   GGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTATCCACAACAGGCAAAAGCCCTAATA                                                                                            >  SRR4302547.126865/1‑94 (MQ=60)
   GGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATA                                                                                            >  SRR4302547.4331763/1‑94 (MQ=60)
    GATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAG                                                                                           >  SRR4302547.3018880/1‑94 (MQ=60)
    GATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAG                                                                                           <  SRR4302547.3592389/94‑1 (MQ=60)
     ATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGA                                                                                          <  SRR4302547.3631734/94‑1 (MQ=60)
     ATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGA                                                                                          <  SRR4302547.1861499/94‑1 (MQ=60)
     ATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGA                                                                                          <  SRR4302547.2319301/94‑1 (MQ=60)
     ATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGA                                                                                          >  SRR4302547.3916292/1‑94 (MQ=60)
     ATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGACTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGA                                                                                          >  SRR4302547.504035/1‑94 (MQ=60)
      TTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAA                                                                                         <  SRR4302547.6309935/94‑1 (MQ=60)
       TTACTGCGCTATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAAC                                                                                        >  SRR4302547.6585674/1‑94 (MQ=60)
       TTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAAC                                                                                        >  SRR4302547.3712128/1‑94 (MQ=60)
       TTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAAC                                                                                        <  SRR4302547.3768540/94‑1 (MQ=60)
       TTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAAC                                                                                        >  SRR4302547.4267232/1‑94 (MQ=60)
        TACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACG                                                                                       <  SRR4302547.2562658/94‑1 (MQ=60)
        TACTGCGCGATCAGGCGATGATCCCGGAAGCTCAGCGCCTGGCACCCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACG                                                                                       <  SRR4302547.1768268/94‑1 (MQ=60)
         ACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGC                                                                                      >  SRR4302547.783922/1‑94 (MQ=60)
         ACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGT                                                                                      >  SRR4302547.2621461/1‑94 (MQ=60)
           TGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTG                                                                                    <  SRR4302547.4452756/94‑1 (MQ=60)
            GCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGG                                                                                   <  SRR4302547.6535065/94‑1 (MQ=60)
             CGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGA                                                                                  >  SRR4302547.5915273/1‑94 (MQ=60)
             CGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGA                                                                                  >  SRR4302547.2280115/1‑94 (MQ=60)
              GCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGAT                                                                                 >  SRR4302547.4356026/1‑94 (MQ=60)
              GCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGAT                                                                                 <  SRR4302547.4285252/94‑1 (MQ=60)
               CGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATG                                                                                >  SRR4302547.2666446/1‑94 (MQ=60)
               CGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATG                                                                                <  SRR4302547.1629821/94‑1 (MQ=60)
               CGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGACCTAATAGAACGCTGGATG                                                                                >  SRR4302547.3329854/1‑94 (MQ=60)
                GATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGC                                                                               >  SRR4302547.1314618/1‑94 (MQ=60)
                 ATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCC                                                                              <  SRR4302547.5276884/94‑1 (MQ=60)
                  TCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCG                                                                             <  SRR4302547.3152612/94‑1 (MQ=60)
                   CAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGG                                                                            <  SRR4302547.3538929/94‑1 (MQ=60)
                    AGGCGATGATCCCGGAAGTTCAGCGCTTGGCACGCCATATTCACGAACGTTACCCACAACGGGCAAAAGCCCTAATAGAACGCTGGATGCCGGA                                                                           <  SRR4302547.3433872/94‑1 (MQ=60)
                    AGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGA                                                                           >  SRR4302547.1067948/1‑94 (MQ=60)
                    AGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGA                                                                           >  SRR4302547.4663931/1‑94 (MQ=60)
                     GGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAG                                                                          >  SRR4302547.6756974/1‑94 (MQ=60)
                       CGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGAC                                                                        <  SRR4302547.2572283/94‑1 (MQ=60)
                       CGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGAC                                                                        <  SRR4302547.5134741/94‑1 (MQ=60)
                        GATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACG                                                                       >  SRR4302547.935121/1‑94 (MQ=60)
                         ATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGG                                                                      >  SRR4302547.2524815/1‑94 (MQ=60)
                         ATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGG                                                                      <  SRR4302547.2628214/94‑1 (MQ=60)
                           GATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGCGAAGGAA                                                                    >  SRR4302547.1629987/1‑94 (MQ=60)
                           GATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAA                                                                    >  SRR4302547.973936/1‑94 (MQ=60)
                           GATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAA                                                                    >  SRR4302547.433556/1‑94 (MQ=60)
                             TCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACG                                                                  <  SRR4302547.1539485/94‑1 (MQ=60)
                              CCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGT                                                                 >  SRR4302547.2653062/1‑94 (MQ=60)
                              CCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGT                                                                 >  SRR4302547.4050262/1‑94 (MQ=60)
                               CCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTT                                                                <  SRR4302547.3174092/94‑1 (MQ=60)
                                CGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGCCGGAACGTTA                                                               >  SRR4302547.5228394/1‑94 (MQ=60)
                                CGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTA                                                               >  SRR4302547.1577506/1‑94 (MQ=60)
                                  GAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACT                                                             <  SRR4302547.1745854/94‑1 (MQ=60)
                                   AAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTC                                                            >  SRR4302547.4954549/1‑94 (MQ=60)
                                   AAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTC                                                            >  SRR4302547.1334884/1‑94 (MQ=60)
                                    AGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCG                                                           <  SRR4302547.2996142/94‑1 (MQ=60)
                                    AGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCG                                                           >  SRR4302547.4121690/1‑94 (MQ=60)
                                    AGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCG                                                           <  SRR4302547.327878/94‑1 (MQ=60)
                                       TCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAAT                                                        <  SRR4302547.160593/94‑1 (MQ=60)
                                       TCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAAT                                                        <  SRR4302547.2717410/94‑1 (MQ=60)
                                       TCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAAT                                                        <  SRR4302547.5403539/94‑1 (MQ=60)
                                        CAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATG                                                       <  SRR4302547.5577456/94‑1 (MQ=60)
                                        CAGCGCCTGCCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATG                                                       <  SRR4302547.3930515/94‑1 (MQ=60)
                                         AGCGCCTGGCACGCCATATTCACGAACGTTTCCCACAACAGGCTAAAGCCCTGATAGAACGCTGGATGCCGGAGAagatccgttgctcgaatgc                                                      >  SRR4302547.5375820/1‑75 (MQ=60)
                                         AGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGC                                                      >  SRR4302547.6036916/1‑94 (MQ=60)
                                         AGCGCCTGGCACGACATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGG                                                      >  SRR4302547.1615220/1‑94 (MQ=60)
                                          GCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCG                                                     <  SRR4302547.5413693/94‑1 (MQ=60)
                                           CGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGT                                                    >  SRR4302547.6321587/1‑94 (MQ=60)
                                           CGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGT                                                    <  SRR4302547.92127/94‑1 (MQ=60)
                                             CCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAA                                                  >  SRR4302547.1454785/1‑94 (MQ=60)
                                               TGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAA                                                >  SRR4302547.918644/1‑94 (MQ=60)
                                               TGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAA                                                >  SRR4302547.5906147/1‑94 (MQ=60)
                                               TGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAA                                                >  SRR4302547.6440155/1‑94 (MQ=60)
                                               TGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAAAGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAA                                                <  SRR4302547.5355116/94‑1 (MQ=60)
                                                GTCACGCCATATTCACGAACGTCACCCACAGCAGGCAAAAGCCCTAATAGAATGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAG                                               <  SRR4302547.3500207/94‑1 (MQ=60)
                                                GGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAG                                               <  SRR4302547.5245401/94‑1 (MQ=60)
                                                 CCACGCCATATTCACGAACGTAACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACTTTACTCGAATGCGTAAAAGG                                              <  SRR4302547.2985809/94‑1 (MQ=60)
                                                  CACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACACTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGC                                             >  SRR4302547.3777669/1‑94 (MQ=60)
                                                   ACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCG                                            >  SRR4302547.2153506/1‑94 (MQ=60)
                                                     GCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGC                                          >  SRR4302547.2183537/1‑94 (MQ=60)
                                                     GCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGC                                          >  SRR4302547.5770583/1‑94 (MQ=60)
                                                     GCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGC                                          >  SRR4302547.1735082/1‑94 (MQ=60)
                                                      CCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCA                                         >  SRR4302547.2487067/1‑94 (MQ=60)
                                                       CATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAG                                        <  SRR4302547.911456/94‑1 (MQ=60)
                                                        ATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGT                                       <  SRR4302547.5716906/94‑1 (MQ=60)
                                                        ATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGT                                       <  SRR4302547.6593435/94‑1 (MQ=60)
                                                        ATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGT                                       <  SRR4302547.212795/94‑1 (MQ=60)
                                                         TATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTG                                      >  SRR4302547.6013702/1‑94 (MQ=60)
                                                         TATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTG                                      <  SRR4302547.181560/94‑1 (MQ=60)
                                                          ATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGA                                     <  SRR4302547.5374710/94‑1 (MQ=60)
                                                          ATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGA                                     >  SRR4302547.1566617/1‑94 (MQ=60)
                                                          ATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGA                                     >  SRR4302547.3745340/1‑94 (MQ=60)
                                                          ATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGA                                     >  SRR4302547.5356563/1‑94 (MQ=60)
                                                          ATTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGA                                     >  SRR4302547.4681878/1‑94 (MQ=60)
                                                           TTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGATCGCTGGATGCCGGAGACGGACCGTTACTCGAATGCGTAAAAGGCGGCAGTGAG                                    <  SRR4302547.3193026/94‑1 (MQ=60)
                                                           TTCACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAG                                    >  SRR4302547.4007739/1‑94 (MQ=60)
                                                              ACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAG                                 >  SRR4302547.3417774/1‑94 (MQ=60)
                                                              ACGAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAG                                 <  SRR4302547.2025312/94‑1 (MQ=60)
                                                                GAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGAC                               >  SRR4302547.4263069/1‑94 (MQ=60)
                                                                GAACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGAC                               >  SRR4302547.3468596/1‑94 (MQ=60)
                                                                 AACGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACC                              >  SRR4302547.5894553/1‑94 (MQ=60)
                                                                   CGTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGC                            >  SRR4302547.1212041/1‑94 (MQ=60)
                                                                    GTTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCC                           <  SRR4302547.5990592/94‑1 (MQ=60)
                                                                     TTACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCA                          >  SRR4302547.2381517/1‑94 (MQ=60)
                                                                       ACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATT                        >  SRR4302547.1807906/1‑94 (MQ=60)
                                                                       ACCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATT                        >  SRR4302547.136669/1‑94 (MQ=60)
                                                                        CCCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTT                       <  SRR4302547.2302274/94‑1 (MQ=60)
                                                                         CCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTC                      <  SRR4302547.3642250/94‑1 (MQ=60)
                                                                         CCACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTC                      >  SRR4302547.6768628/1‑94 (MQ=60)
                                                                          CACAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCA                     <  SRR4302547.6785225/94‑1 (MQ=60)
                                                                            CAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGG                   <  SRR4302547.1566348/94‑1 (MQ=60)
                                                                            CAACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGG                   <  SRR4302547.5076342/94‑1 (MQ=60)
                                                                             AACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGT                  >  SRR4302547.1706278/1‑94 (MQ=60)
                                                                              ACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTT                 <  SRR4302547.126364/94‑1 (MQ=60)
                                                                              ACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTT                 <  SRR4302547.2410820/94‑1 (MQ=60)
                                                                              ACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTT                 <  SRR4302547.3711386/94‑1 (MQ=60)
                                                                              ACAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTT                 >  SRR4302547.203392/1‑94 (MQ=60)
                                                                               CAGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTA                >  SRR4302547.5325945/1‑94 (MQ=60)
                                                                                AGGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTAC               >  SRR4302547.558841/1‑94 (MQ=60)
                                                                                 GGCAAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACC              <  SRR4302547.4423252/94‑1 (MQ=60)
                                                                                    AAAAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGGAGTGAGAAaaacgccccctttctcttgctct           >  SRR4302547.5902156/1‑71 (MQ=60)
                                                                                      AAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACC         >  SRR4302547.6542773/1‑94 (MQ=60)
                                                                                      AAGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACC         <  SRR4302547.5494428/94‑1 (MQ=60)
                                                                                       AGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCT        <  SRR4302547.5304997/94‑1 (MQ=60)
                                                                                       AGCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCA        <  SRR4302547.2474636/94‑1 (MQ=60)
                                                                                        GCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCTT       >  SRR4302547.4794784/1‑94 (MQ=60)
                                                                                        GCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCTT       <  SRR4302547.3377454/94‑1 (MQ=60)
                                                                                        GCCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCTT       <  SRR4302547.6153535/94‑1 (MQ=60)
                                                                                         CCCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCTTC      >  SRR4302547.68926/1‑94 (MQ=60)
                                                                                          CCTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCTTCC     <  SRR4302547.5599595/94‑1 (MQ=60)
                                                                                           CTAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCTTCCT    >  SRR4302547.1792527/1‑94 (MQ=60)
                                                                                            TAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCTTCCTG   >  SRR4302547.1417230/1‑94 (MQ=60)
                                                                                            TAATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCTTCCTG   <  SRR4302547.207508/94‑1 (MQ=60)
                                                                                             AATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCTTCCTGC  <  SRR4302547.4675951/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAAAAGGCGGCAGTGAGAAGACCGCCATTTCAGGTTACCCTACCTTCCTGC  >  NC_000913/3824780‑3824966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑