Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,874,594	A→G	intergenic (‑14/+101)	yidA ← / ← b3698	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,874,594	0	A	G	83.6%	629.9 / 112.4	256	intergenic (‑14/+101)	yidA/b3698	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (25/17); new base G (121/93); total (146/110)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

Only 100 of 261 total aligned reads displayed.

AACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCC  >  NC_000913/3874506‑3874686

aaCGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGAtt                                                                                          <  1:5342169/93‑1 (MQ=255)

aaCGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGAtt                                                                                          <  1:3435171/93‑1 (MQ=255)

aaCGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGAtt                                                                                          <  1:3688449/93‑1 (MQ=255)

     cggGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTc                                                                                     <  1:6604847/93‑1 (MQ=255)

     cggGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTc                                                                                     <  2:4881536/93‑1 (MQ=255)

     cggGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTc                                                                                     >  2:355290/1‑93 (MQ=255)

      gtgTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTcc                                                                                    <  2:1541788/91‑1 (MQ=255)

      gggTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTcc                                                                                    <  2:5541500/93‑1 (MQ=255)

       ggTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCt                                                                                   <  2:5010182/93‑1 (MQ=255)

        gTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTg                                                                                  >  2:4457418/1‑93 (MQ=255)

           aaaTGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGAcc                                                                               >  1:2030263/1‑93 (MQ=255)

            aaTGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGAccc                                                                              >  2:1727974/1‑93 (MQ=255)

             aTGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCa                                                                             <  1:1166646/93‑1 (MQ=255)

             aTGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCa                                                                             <  2:3259671/93‑1 (MQ=255)

             aTGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCa                                                                             <  2:6088686/93‑1 (MQ=255)

              tGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCAt                                                                            <  1:4518377/93‑1 (MQ=255)

              tGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCAt                                                                            <  2:2497190/93‑1 (MQ=255)

               ggTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATg                                                                           >  1:6486187/1‑93 (MQ=255)

                 tgtgATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCt                                                                         <  1:4563706/93‑1 (MQ=255)

                  gtgATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTa                                                                        >  2:3487947/1‑93 (MQ=255)

                   tgATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTaa                                                                       >  2:4651532/1‑93 (MQ=255)

                   tgATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTaa                                                                       >  1:772026/1‑93 (MQ=255)

                   tgATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTaa                                                                       >  1:3286143/1‑93 (MQ=255)

                    gATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAAc                                                                      >  1:1486705/1‑93 (MQ=255)

                     aTCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAAcg                                                                     >  1:3617180/1‑93 (MQ=255)

                       cGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAAcgcg                                                                   >  2:2390766/1‑93 (MQ=255)

                       cGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAAcgcg                                                                   >  1:2763404/1‑93 (MQ=255)

                       cGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAAcgcg                                                                   <  1:445222/93‑1 (MQ=255)

                         ggCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGAt                                                                 >  2:620730/1‑93 (MQ=255)

                           cagcagAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTc                                                               <  2:5215895/93‑1 (MQ=255)

                             gcagAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCg                                                             >  2:6423644/1‑93 (MQ=255)

                             gcagAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCg                                                             >  1:1747437/1‑93 (MQ=255)

                              cagAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGc                                                            <  1:3970700/93‑1 (MQ=255)

                                gAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTc                                                          >  1:4190486/1‑93 (MQ=255)

                                gAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTc                                                          <  2:186272/93‑1 (MQ=255)

                                gAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTc                                                          <  2:3167794/93‑1 (MQ=255)

                                gAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGCTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTc                                                          >  2:1050478/1‑93 (MQ=255)

                                   gggTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCaaa                                                       >  2:1255950/1‑93 (MQ=255)

                                   gggTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCaaa                                                       >  2:1133468/1‑93 (MQ=255)

                                     gTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCaaaaa                                                     <  1:987008/93‑1 (MQ=255)

                                       gCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATc                                                   >  2:5308855/1‑93 (MQ=255)

                                        ccatccatATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCa                                                  <  1:3866414/93‑1 (MQ=255)

                                        ccatccatATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCa                                                  <  2:3590948/93‑1 (MQ=255)

                                         catccatATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAACATCAg                                                 >  2:2732489/1‑93 (MQ=255)

                                         catccatATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAg                                                 <  1:1757929/93‑1 (MQ=255)

                                          atccatATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGt                                                >  2:2569490/1‑93 (MQ=255)

                                           tccatATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTa                                               <  2:4286064/93‑1 (MQ=255)

                                            ccatATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTac                                              >  2:3731253/1‑93 (MQ=255)

                                             catATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTaca                                             >  1:1945973/1‑93 (MQ=255)

                                                atCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTacaaca                                          >  2:1947144/1‑93 (MQ=255)

                                                 tCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAacac                                         <  2:5164023/93‑1 (MQ=255)

                                                  cGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACAcc                                        <  1:5574143/93‑1 (MQ=255)

                                                   gATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACAccc                                       >  2:2217585/1‑93 (MQ=255)

                                                   gATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACAccc                                       >  1:5055407/1‑93 (MQ=255)

                                                    aTAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCg                                      >  1:215455/1‑93 (MQ=255)

                                                     tAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGa                                     >  1:6377825/1‑93 (MQ=255)

                                                     tAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGa                                     >  1:2231377/1‑93 (MQ=255)

                                                      aGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAg                                    <  2:4397585/93‑1 (MQ=255)

                                                      aGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAg                                    >  2:4962003/1‑93 (MQ=255)

                                                      aGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAg                                    <  2:3976412/93‑1 (MQ=255)

                                                       gCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAgg                                   >  2:6330527/1‑93 (MQ=255)

                                                        cAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAggg                                  >  1:4740920/1‑93 (MQ=255)

                                                         aaTGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGa                                 >  1:5447674/1‑93 (MQ=255)

                                                          aTGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAACAATCAGTACAACACCCGAGGGaa                                >  2:6012238/1‑93 (MQ=255)

                                                           tGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGaaa                               >  2:2895293/1‑93 (MQ=255)

                                                            gAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGaaaa                              >  1:2673202/1‑93 (MQ=255)

                                                             aGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAg                             <  2:40360/93‑1 (MQ=255)

                                                              gTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAgg                            >  1:809045/1‑93 (MQ=255)

                                                               tttAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACTCCCGAGGGAAAAggg                           <  1:5830226/93‑1 (MQ=255)

                                                               tttAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAggg                           <  1:4910846/93‑1 (MQ=255)

                                                                ttAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAggag                          >  1:6259588/1‑91 (MQ=255)

                                                                 tAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCATTACACCCCCCGAGGGAAAAggggg                         <  1:2177518/93‑1 (MQ=255)

                                                                  aaTAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGa                        >  2:4233163/1‑93 (MQ=255)

                                                                  aaTAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGa                        <  1:4255235/93‑1 (MQ=255)

                                                                  aaTAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGa                        >  2:5394583/1‑93 (MQ=255)

                                                                  aaTAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGa                        >  1:1028403/1‑93 (MQ=255)

                                                                   aTAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGAt                       >  1:4800894/1‑93 (MQ=255)

                                                                   aTAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGAt                       <  2:34102/93‑1 (MQ=255)

                                                                     aGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGc                     >  1:1588221/1‑93 (MQ=255)

                                                                      gCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCa                    >  1:1299229/1‑93 (MQ=255)

                                                                        cATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAAc                  >  1:5760968/1‑93 (MQ=255)

                                                                        cATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAAc                  >  1:4969658/1‑93 (MQ=255)

                                                                        cATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAAc                  >  2:1639340/1‑93 (MQ=255)

                                                                        cATGAGATCCCCAGGGGGATTGGTTCCTGACCCATGCTAACGCGATTCAGCTCAAAAATCAGTACACCACCCGAGGGAAAAGGGGGATGCAAc                  >  1:3162726/1‑93 (MQ=255)

                                                                          tGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAAcgc                >  2:217047/1‑93 (MQ=255)

                                                                           gAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAAcgcg               >  2:1888950/1‑93 (MQ=255)

                                                                            aGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAAcgcgc              >  1:6472118/1‑93 (MQ=255)

                                                                            aGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAAcgcgc              <  1:3542564/93‑1 (MQ=255)

                                                                             gTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAAcgcgcg             >  1:1550267/1‑93 (MQ=255)

                                                                              ttCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGt            <  1:6121385/93‑1 (MQ=255)

                                                                               tCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGtt           <  2:5871382/93‑1 (MQ=255)

                                                                               tCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGtt           <  2:1489472/93‑1 (MQ=255)

                                                                                ccccAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTg          <  2:3115121/93‑1 (MQ=255)

                                                                                 cccAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGc         >  2:1981686/1‑93 (MQ=255)

                                                                                  ccAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCg        >  1:2512609/1‑93 (MQ=255)

                                                                                   cAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGt       >  2:6102612/1‑93 (MQ=255)

                                                                                   cAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGt       <  2:670809/93‑1 (MQ=255)

                                                                                   cAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGt       <  1:1329645/93‑1 (MQ=255)

                                                                                     ggTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGc     >  2:3497766/1‑93 (MQ=255)

                                                                                     ggTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGc     >  2:2971773/1‑93 (MQ=255)

AACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCC  >  NC_000913/3874506‑3874686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCC  >  NC_000913/3874506‑3874686

AACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTG                                                                                         <  SRR4302547.3505204/94‑1 (MQ=60)

AACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTG                                                                                         <  SRR4302547.5452301/94‑1 (MQ=60)

AACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTG                                                                                         <  SRR4302547.3763750/94‑1 (MQ=60)

   cgcgggtgtattagtagtATCGGGCGGCAGAAGAATGCCATCCATATCGATAGCAATGAGTTTAATAGCATTGAGTTTCCCAGGTCGATTGaat                                                                                      <  SRR4302547.2670582/76‑4 (MQ=37)

   GGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCCTCCATATCGATAGCAATGAGTTTAATAGGCATGAGTTCCCCAGGTGGATTGGGT                                                                                      >  SRR4302547.1472003/1‑94 (MQ=60)

   GGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGGGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTT                                                                                      >  SRR4302547.4937843/1‑94 (MQ=60)

     CGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCC                                                                                    <  SRR4302547.6742336/94‑1 (MQ=60)

      GGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCT                                                                                   >  SRR4302547.696446/1‑94 (MQ=60)

        GTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGA                                                                                 >  SRR4302547.189403/1‑94 (MQ=60)

         TGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGAC                                                                                >  SRR4302547.5105202/1‑94 (MQ=60)

         TGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGAC                                                                                <  SRR4302547.4257361/94‑1 (MQ=60)

          GAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACC                                                                               >  SRR4302547.2070694/1‑94 (MQ=60)

           AAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCC                                                                              >  SRR4302547.4599939/1‑94 (MQ=60)

           AAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCC                                                                              >  SRR4302547.6729292/1‑94 (MQ=60)

             ATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCAT                                                                            <  SRR4302547.1190244/94‑1 (MQ=60)

             ATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCAT                                                                            >  SRR4302547.1583622/1‑94 (MQ=60)

             ATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCAT                                                                            <  SRR4302547.6522054/94‑1 (MQ=60)

              TGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATG                                                                           >  SRR4302547.6621224/1‑94 (MQ=60)

              TGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATG                                                                           <  SRR4302547.4611722/94‑1 (MQ=60)

               GGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGC                                                                          >  SRR4302547.3578302/1‑94 (MQ=60)

                 TGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTA                                                                        <  SRR4302547.4658122/94‑1 (MQ=60)

                  GTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAA                                                                       >  SRR4302547.786615/1‑94 (MQ=60)

                  GTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAA                                                                       <  SRR4302547.6549714/94‑1 (MQ=60)

                  GTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAA                                                                       >  SRR4302547.3352053/1‑94 (MQ=60)

                   TGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAAC                                                                      <  SRR4302547.2789157/94‑1 (MQ=60)

                   TGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAAC                                                                      >  SRR4302547.1516386/1‑94 (MQ=60)

                   TGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAAC                                                                      >  SRR4302547.444666/1‑94 (MQ=60)

                    GATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACG                                                                     >  SRR4302547.3690974/1‑94 (MQ=60)

                     ATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGC                                                                    <  SRR4302547.1647484/94‑1 (MQ=60)

                      TCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCG                                                                   >  SRR4302547.2818784/1‑94 (MQ=60)

                       CGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGA                                                                  >  SRR4302547.4664131/1‑94 (MQ=60)

                       CGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGA                                                                  <  SRR4302547.453516/94‑1 (MQ=60)

                        GGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGAT                                                                 <  SRR4302547.5029546/94‑1 (MQ=60)

                           CAGCAGAAGGGTGCCATCCATATCGATAGCAAAGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCC                                                              >  SRR4302547.5415770/1‑94 (MQ=60)

                            AGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCG                                                             >  SRR4302547.1781912/1‑94 (MQ=60)

                            AGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCG                                                             >  SRR4302547.3277004/1‑94 (MQ=60)

                            AGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCG                                                             <  SRR4302547.1539146/94‑1 (MQ=60)

                            AGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACCCGATTCCG                                                             >  SRR4302547.5202541/1‑94 (MQ=60)

                              CAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCT                                                           <  SRR4302547.4101265/94‑1 (MQ=60)

                              CAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCT                                                           <  SRR4302547.4051460/94‑1 (MQ=60)

                              CAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCT                                                           >  SRR4302547.4313366/1‑94 (MQ=60)

                               AGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTC                                                          >  SRR4302547.4275945/1‑94 (MQ=60)

                               AGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTC                                                          >  SRR4302547.5924044/1‑94 (MQ=60)

                               AGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTC                                                          >  SRR4302547.3709006/1‑94 (MQ=60)

                               AGAAGGGTGACATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTC                                                          >  SRR4302547.6618439/1‑94 (MQ=60)

                                GAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCA                                                         <  SRR4302547.6069989/94‑1 (MQ=60)

                                GAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCA                                                         <  SRR4302547.4588208/94‑1 (MQ=60)

                                 AAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAA                                                        >  SRR4302547.5666152/1‑94 (MQ=60)

                                 AAGGGTGCCATCCATATCGATAGCAATGAGGTTAATAGACATGAGTTCCCCAGGTGGGTTGGTTGCTGACCCAAGCTAAAGCGATTtcccttaa                                                        >  SRR4302547.6665617/1‑86 (MQ=60)

                                     GTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAAT                                                    <  SRR4302547.1006216/94‑1 (MQ=60)

                                      TGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATC                                                   >  SRR4302547.90897/1‑94 (MQ=60)

                                       TCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCA                                                  <  SRR4302547.2991499/94‑1 (MQ=60)

                                       GCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCA                                                  <  SRR4302547.1411295/94‑1 (MQ=60)

                                        CCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAG                                                 <  SRR4302547.3945120/94‑1 (MQ=60)

                                         CATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGT                                                <  SRR4302547.1792742/94‑1 (MQ=60)

                                          ATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTA                                               >  SRR4302547.6243480/1‑94 (MQ=60)

                                          ATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTA                                               >  SRR4302547.3753806/1‑94 (MQ=60)

                                           TCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTAC                                              >  SRR4302547.2449312/1‑94 (MQ=60)

                                            CCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACA                                             >  SRR4302547.1984551/1‑94 (MQ=60)

                                             CATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAA                                            >  SRR4302547.2096129/1‑94 (MQ=60)

                                             CATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAA                                            <  SRR4302547.6255415/94‑1 (MQ=60)

                                             CATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAA                                            >  SRR4302547.1241493/1‑94 (MQ=60)

                                              ATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAAC                                           <  SRR4302547.5613977/94‑1 (MQ=60)

                                               TATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACA                                          >  SRR4302547.3183144/1‑94 (MQ=60)

                                                  CGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCC                                       >  SRR4302547.4737517/1‑94 (MQ=60)

                                                  CGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCC                                       <  SRR4302547.5689075/94‑1 (MQ=60)

                                                  CGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCC                                       >  SRR4302547.1246363/1‑94 (MQ=60)

                                                  CGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCC                                       >  SRR4302547.5159758/1‑94 (MQ=60)

                                                   GATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCG                                      >  SRR4302547.219515/1‑94 (MQ=60)

                                                   GATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCG                                      >  SRR4302547.906778/1‑94 (MQ=60)

                                                   GATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCA                                      >  SRR4302547.3494660/1‑94 (MQ=60)

                                                    ATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGA                                     <  SRR4302547.496588/94‑1 (MQ=60)

                                                    ATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGA                                     >  SRR4302547.2276352/1‑94 (MQ=60)

                                                    ATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGA                                     >  SRR4302547.6510916/1‑94 (MQ=60)

                                                     TAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAG                                    <  SRR4302547.3583521/94‑1 (MQ=60)

                                                     TAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAG                                    <  SRR4302547.1338449/94‑1 (MQ=60)

                                                      AGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGG                                   <  SRR4302547.2045509/94‑1 (MQ=60)

                                                      AGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGG                                   >  SRR4302547.1905054/1‑94 (MQ=60)

                                                       GCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGG                                  >  SRR4302547.4839176/1‑94 (MQ=60)

                                                       GCAATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGG                                  >  SRR4302547.3789809/1‑94 (MQ=60)

                                                        CAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA                                 >  SRR4302547.2199283/1‑94 (MQ=60)

                                                        CAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA                                 >  SRR4302547.5560242/1‑94 (MQ=60)

                                                         AATGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAA                                >  SRR4302547.663545/1‑94 (MQ=60)

                                                           TGAGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAA                              >  SRR4302547.2726899/1‑94 (MQ=60)

                                                             AGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGG                            <  SRR4302547.2016028/94‑1 (MQ=60)

                                                             AGTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGG                            >  SRR4302547.824373/1‑94 (MQ=60)

                                                             AGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAAGCAGTACAACACCCGAGGGAAAAGG                            <  SRR4302547.701498/94‑1 (MQ=60)

                                                              GTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGG                           >  SRR4302547.4432169/1‑94 (MQ=60)

                                                              GTTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGG                           <  SRR4302547.5541616/94‑1 (MQ=60)

                                                               TTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACTCCCGAGGGAAAAGGGG                          <  SRR4302547.5950864/94‑1 (MQ=60)

                                                               TTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG                          <  SRR4302547.5012535/94‑1 (MQ=60)

                                                               TTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG                          <  SRR4302547.1786098/94‑1 (MQ=60)

                                                               TTTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGAG                          >  SRR4302547.6389638/1‑94 (MQ=60)

                                                                TTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGG                         <  SRR4302547.1633728/94‑1 (MQ=60)

                                                                TTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGG                         <  SRR4302547.3840745/94‑1 (MQ=60)

                                                                TTAATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGG                         <  SRR4302547.6265970/94‑1 (MQ=60)

                                                                 TAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCATTACACCCCCCGAGGGAAAAGGGGGA                        <  SRR4302547.2221122/94‑1 (MQ=60)

                                                                 TAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGA                        >  SRR4302547.1048603/1‑94 (MQ=60)

                                                                  AATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGAT                       >  SRR4302547.4900379/1‑94 (MQ=60)

                                                                  AATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGAT                       <  SRR4302547.4342305/94‑1 (MQ=60)

                                                                  AATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAAGCGGAT                       >  SRR4302547.6469522/1‑94 (MQ=60)

                                                                   ATAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATG                      >  SRR4302547.2584947/1‑94 (MQ=60)

                                                                    TAGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGC                     >  SRR4302547.1619782/1‑94 (MQ=60)

                                                                     AGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGACAAGGGGGATGCA                    >  SRR4302547.4012010/1‑94 (MQ=60)

                                                                     AGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCA                    <  SRR4302547.4865935/94‑1 (MQ=60)

                                                                     AGCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCA                    <  SRR4302547.4630755/94‑1 (MQ=60)

                                                                     AGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCA                    >  SRR4302547.1325380/1‑94 (MQ=60)

                                                                      GCCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAA                   >  SRR4302547.2531873/1‑94 (MQ=60)

                                                                       CCATGAGTTCCCCAGGTGGATTGGTTCTTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAAC                  <  SRR4302547.5822897/94‑1 (MQ=60)

                                                                       CCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAAC                  >  SRR4302547.5072488/1‑94 (MQ=60)

                                                                       CCATGAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAAC                  >  SRR4302547.5880112/1‑94 (MQ=60)

                                                                       CCATGAGATCCCCAGGGGGATTGGTTCCTGACCCATGCTAACGCGATTCAGCTCAAAAATCAGTACACCACCCGAGGGAAAAGGGGGATGCAAC                  >  SRR4302547.3225652/1‑94 (MQ=60)

                                                                           GAGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGC              >  SRR4302547.6606880/1‑94 (MQ=60)

                                                                            GGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCG             <  SRR4302547.799605/94‑1 (MQ=60)

                                                                            AGTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCG             <  SRR4302547.3614879/94‑1 (MQ=60)

                                                                            AGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCG             >  SRR4302547.1101831/1‑94 (MQ=60)

                                                                            AGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCG             >  SRR4302547.1581131/1‑94 (MQ=60)

                                                                             GTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGT            <  SRR4302547.1555738/94‑1 (MQ=60)

                                                                             GTTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGT            <  SRR4302547.3420084/94‑1 (MQ=60)

                                                                              TTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTT           <  SRR4302547.6248376/94‑1 (MQ=60)

                                                                              GTCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTT           <  SRR4302547.2575771/94‑1 (MQ=60)

                                                                               TCCCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTG          <  SRR4302547.2216541/94‑1 (MQ=60)

                                                                                CCACAGGTGGATTGGTTCCTGACCGCTGCTAACGCGATTCCGCTAAAAAACCAGTACAACACCCGAGGGAAACGGGGGATGCAACGCGCGTTGC         <  SRR4302547.2239242/94‑1 (MQ=60)

                                                                                 CCCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCG        >  SRR4302547.5066104/1‑94 (MQ=60)

                                                                                 CCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCG        >  SRR4302547.2563396/1‑94 (MQ=60)

                                                                                  CCAGGTGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGT       <  SRR4302547.3634852/94‑1 (MQ=60)

                                                                                   CAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTG      <  SRR4302547.1356366/94‑1 (MQ=60)

                                                                                       TGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCC  >  SRR4302547.2541245/1‑94 (MQ=60)

                                                                                       TGGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCC  >  SRR4302547.5633781/1‑94 (MQ=60)

                                                                                       TAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCC  >  SRR4302547.5863887/1‑94 (MQ=60)

AACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCC  >  NC_000913/3874506‑3874686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`