Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,896,196	T→C	C182C (TGT→TGC)	yieJ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,896,196	0	T	C	86.7%	687.7 / 89.7	256	C182C (TGT→TGC)	yieJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (18/16); new base C (114/107); total (133/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

Only 100 of 262 total aligned reads displayed.

GAAGAAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCT  >  NC_000913/3896104‑3896287

  agaagaTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGcct                                                                                           >  2:1915983/1‑93 (MQ=255)

     agaTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCa                                                                                        >  1:6520418/1‑93 (MQ=255)

      gaTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCAc                                                                                       >  1:2264210/1‑93 (MQ=255)

      gaTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCAc                                                                                       >  1:754783/1‑93 (MQ=255)

       aTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACt                                                                                      <  1:5006494/93‑1 (MQ=255)

        ttGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTg                                                                                     <  1:5070427/93‑1 (MQ=255)

           tgAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCgg                                                                                  <  2:2604107/93‑1 (MQ=255)

            gagaATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGc                                                                                 >  2:2638297/1‑93 (MQ=255)

             agaATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCa                                                                                >  1:4519865/1‑93 (MQ=255)

               aaTTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAg                                                                              <  2:1415890/93‑1 (MQ=255)

                aTTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGc                                                                             >  2:3856609/1‑93 (MQ=255)

                  ttCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTg                                                                           <  2:5099101/93‑1 (MQ=255)

                   tCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTga                                                                          <  1:42855/93‑1 (MQ=255)

                   tCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTga                                                                          >  2:748661/1‑93 (MQ=255)

                   tCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTga                                                                          <  2:6348893/93‑1 (MQ=255)

                     ggTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTgaga                                                                        >  1:3090228/1‑93 (MQ=255)

                      gTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGAc                                                                       >  2:3624135/1‑93 (MQ=255)

                      gTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGAc                                                                       <  2:3656618/93‑1 (MQ=255)

                      gTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGAc                                                                       <  2:5231445/93‑1 (MQ=255)

                       tATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACt                                                                      >  2:5461489/1‑93 (MQ=255)

                       tATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACt                                                                      >  2:541893/1‑93 (MQ=255)

                        aTTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACtg                                                                     <  1:214583/93‑1 (MQ=255)

                        aTTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACtg                                                                     <  1:5289025/93‑1 (MQ=255)

                        aTTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACtg                                                                     <  1:4742746/93‑1 (MQ=255)

                          tAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACtgtg                                                                   <  1:4428889/93‑1 (MQ=255)

                          tAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACtgtg                                                                   <  2:4719705/93‑1 (MQ=255)

                           aGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTgg                                                                  <  1:4551704/93‑1 (MQ=255)

                            gAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTggg                                                                 <  1:5389544/93‑1 (MQ=255)

                              aaTTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGt                                                               <  2:575208/93‑1 (MQ=255)

                              aaTTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGt                                                               <  2:2331026/93‑1 (MQ=255)

                                ttCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGa                                                             >  1:4011801/1‑93 (MQ=255)

                                ttCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGa                                                             >  2:3302755/1‑93 (MQ=255)

                                ttCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGa                                                             >  1:382654/1‑93 (MQ=255)

                                    gATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGAtttt                                                         >  1:1132617/1‑93 (MQ=255)

                                    gATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGAtttt                                                         >  2:2816853/1‑93 (MQ=255)

                                      tCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTc                                                       >  2:20711/1‑93 (MQ=255)

                                       cTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCg                                                      <  1:2546086/93‑1 (MQ=255)

                                       cTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCg                                                      >  2:4481234/1‑93 (MQ=255)

                                        tAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGt                                                     >  1:1561898/1‑93 (MQ=255)

                                          gCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAg                                                   >  2:2483784/1‑93 (MQ=255)

                                           cTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGt                                                  <  2:2926132/93‑1 (MQ=255)

                                            tAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGtt                                                 >  2:2538037/1‑93 (MQ=255)

                                            tAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGtt                                                 >  1:874214/1‑93 (MQ=255)

                                             aaaTGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTa                                                >  2:325545/1‑93 (MQ=255)

                                              aaTGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTAt                                               >  2:1832070/1‑93 (MQ=255)

                                              aaTGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTAt                                               >  2:2615513/1‑93 (MQ=255)

                                                tGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTAttt                                             <  2:6545835/93‑1 (MQ=255)

                                                tGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTAttt                                             <  2:6052976/93‑1 (MQ=255)

                                                tGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTAttt                                             <  2:6292280/93‑1 (MQ=255)

                                                  ccTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTaa                                           <  2:6315641/93‑1 (MQ=255)

                                                  ccTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTaa                                           >  2:3063271/1‑93 (MQ=255)

                                                  ccTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTaa                                           >  2:2844606/1‑93 (MQ=255)

                                                    tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAaat                                         <  2:6625968/93‑1 (MQ=255)

                                                     gCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAaata                                        >  1:3474176/1‑93 (MQ=255)

                                                      cAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAaataa                                       <  2:5047951/93‑1 (MQ=255)

                                                      cAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAaataa                                       <  1:4823182/93‑1 (MQ=255)

                                                       aaaaGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAaataat                                      >  1:3740053/1‑93 (MQ=255)

                                                       aaaaGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAaataat                                      >  2:5287919/1‑93 (MQ=255)

                                                        aaaGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATg                                     >  1:5623997/1‑93 (MQ=255)

                                                        aaaGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATg                                     >  1:5316295/1‑93 (MQ=255)

                                                         aaGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATga                                    <  2:3217641/93‑1 (MQ=255)

                                                          aGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATgag                                   <  2:3805596/93‑1 (MQ=255)

                                                          aGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATgag                                   >  1:1076239/1‑93 (MQ=255)

                                                          aGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATgag                                   >  2:2285017/1‑93 (MQ=255)

                                                           gggTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATgaga                                  >  1:2994071/1‑93 (MQ=255)

                                                           gggTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATgaga                                  <  1:1120008/93‑1 (MQ=255)

                                                           gggTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATgaga                                  >  2:4861353/1‑93 (MQ=255)

                                                           gggTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATgaga                                  <  2:3462996/93‑1 (MQ=255)

                                                            ggtggtCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGaa                                 <  2:2963141/93‑1 (MQ=255)

                                                             gtggtCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAAc                                <  2:318478/93‑1 (MQ=255)

                                                             gtggtCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAAc                                <  1:4872233/93‑1 (MQ=255)

                                                              tggtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACa                               <  1:4235979/93‑1 (MQ=255)

                                                              tggtCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACa                               >  1:466583/1‑93 (MQ=255)

                                                               ggtCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAg                              >  2:1246597/1‑93 (MQ=255)

                                                               ggtCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAg                              <  1:312629/93‑1 (MQ=255)

                                                                gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAgg                             >  1:1002259/1‑93 (MQ=255)

                                                                gtCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAgg                             >  1:1410306/1‑93 (MQ=255)

                                                                  cATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAggac                           >  1:6152192/1‑91 (MQ=255)

                                                                   aTTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCg                          <  1:3996372/93‑1 (MQ=255)

                                                                   aTTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGGGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCg                          >  1:1679150/1‑93 (MQ=255)

                                                                    ttGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCgg                         >  1:5126569/1‑93 (MQ=255)

                                                                     tGTCAGGGTTATCTCTTCCGCTGCCTCCGCTGCGGCAAGCTTAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGa                        <  2:1592246/93‑1 (MQ=255)

                                                                     tGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGa                        <  1:6385664/93‑1 (MQ=255)

                                                                      gTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAg                       >  1:3220873/1‑93 (MQ=255)

                                                                       tCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGc                      >  1:283093/1‑93 (MQ=255)

                                                                        cAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCg                     >  1:355475/1‑93 (MQ=255)

                                                                         aGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGt                    >  1:4190421/1‑93 (MQ=255)

                                                                            gTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAAt                 >  1:5551712/1‑93 (MQ=255)

                                                                              tATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTc               >  1:6086687/1‑93 (MQ=255)

                                                                              tATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTc               >  2:1010526/1‑93 (MQ=255)

                                                                                tctcTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTcac             >  2:4339058/1‑93 (MQ=255)

                                                                                  tctTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTcacac           <  1:2901291/93‑1 (MQ=255)

                                                                                   ctTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTcacaca          <  2:4110672/93‑1 (MQ=255)

                                                                                     tCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATc        <  2:5601094/93‑1 (MQ=255)

                                                                                     tCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGCACGGAGCGTAATTcacacacc        >  2:4619600/1‑91 (MQ=255)

                                                                                      ccGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCTGAGCGTAATTCACACATcc       >  2:6113060/1‑93 (MQ=255)

                                                                                        gCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCgg     >  2:2394947/1‑93 (MQ=255)

                                                                                        gCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCgg     >  2:400848/1‑93 (MQ=255)

                                                                                        gCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCgg     <  1:3350063/93‑1 (MQ=255)

                                                                                         cTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGc    >  1:596357/1‑93 (MQ=255)

GAAGAAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCT  >  NC_000913/3896104‑3896287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGAAGAAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTT  >  NC_000913/3896103‑3896288

TGAAGAAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGT                                                                                              >  SRR4302547.1116591/1‑94 (MQ=60)

TGAAGAAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGC                                                                                              <  SRR4302547.1597273/94‑1 (MQ=60)

  AAGAAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCT                                                                                            >  SRR4302547.4525882/1‑94 (MQ=60)

  AAGAAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCT                                                                                            <  SRR4302547.4640414/94‑1 (MQ=60)

   AGAAGATTGGGAGAAGTTCGGTATTAGAAATTCTGATATAGCTAAATGCCTGCAAAAGGGCGGTCATTGTCAGGGTTATCTCTTCCGGTGGCTC                                                                                           >  SRR4302547.781824/1‑94 (MQ=60)

     AAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCA                                                                                         <  SRR4302547.3840124/94‑1 (MQ=60)

     AAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCA                                                                                         >  SRR4302547.6656166/1‑94 (MQ=60)

      AGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCAC                                                                                        >  SRR4302547.769005/1‑94 (MQ=60)

      AGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCAC                                                                                        >  SRR4302547.3342347/1‑94 (MQ=60)

      AGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCAC                                                                                        >  SRR4302547.2309970/1‑94 (MQ=60)

        ATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTG                                                                                      <  SRR4302547.5110009/94‑1 (MQ=60)

        ATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTG                                                                                      <  SRR4302547.3079469/94‑1 (MQ=60)

         TTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGC                                                                                     <  SRR4302547.5175046/94‑1 (MQ=60)

          TGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCG                                                                                    >  SRR4302547.1155140/1‑94 (MQ=60)

          TGTAAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCG                                                                                    <  SRR4302547.35374/94‑1 (MQ=60)

             GAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCA                                                                                 >  SRR4302547.4613246/1‑94 (MQ=60)

             GAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCA                                                                                 >  SRR4302547.6695576/1‑94 (MQ=60)

                   TTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGA                                                                           <  SRR4302547.4455287/94‑1 (MQ=60)

                   TTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGA                                                                           <  SRR4302547.5094582/94‑1 (MQ=60)

                    TCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAG                                                                          <  SRR4302547.43643/94‑1 (MQ=60)

                    TCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAG                                                                          <  SRR4302547.4808314/94‑1 (MQ=60)

                    TCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAG                                                                          <  SRR4302547.90279/94‑1 (MQ=60)

                     CGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGC                                                                         >  SRR4302547.5390250/1‑94 (MQ=60)

                     CGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGA                                                                         >  SRR4302547.3151517/1‑94 (MQ=60)

                     CGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGA                                                                         >  SRR4302547.76452/1‑94 (MQ=60)

                       GTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACT                                                                       >  SRR4302547.3482450/1‑94 (MQ=60)

                       GTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACT                                                                       >  SRR4302547.794508/1‑94 (MQ=60)

                         ATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGT                                                                     >  SRR4302547.4971680/1‑94 (MQ=60)

                         ATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGT                                                                     <  SRR4302547.218634/94‑1 (MQ=60)

                         ATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGT                                                                     <  SRR4302547.4841029/94‑1 (MQ=60)

                         ATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGT                                                                     <  SRR4302547.2726638/94‑1 (MQ=60)

                         ATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGT                                                                     <  SRR4302547.5398137/94‑1 (MQ=60)

                          TTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTG                                                                    <  SRR4302547.5547643/94‑1 (MQ=60)

                           TAGAATTTCGGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGG                                                                   <  SRR4302547.4267680/94‑1 (MQ=60)

                           TAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGG                                                                   <  SRR4302547.24309/94‑1 (MQ=60)

                           TAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGG                                                                   <  SRR4302547.4520404/94‑1 (MQ=60)

                           TAGAAATTCTGATCTAGCTAAATGACTGCAAAAGGGGGGTCATTGTCAGGGGTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGG                                                                   >  SRR4302547.4352397/1‑94 (MQ=60)

                            AGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGG                                                                  <  SRR4302547.4237704/94‑1 (MQ=60)

                            AGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGG                                                                  <  SRR4302547.4645850/94‑1 (MQ=60)

                            AGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGG                                                                  >  SRR4302547.5125905/1‑94 (MQ=60)

                             GAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGG                                                                 <  SRR4302547.5500842/94‑1 (MQ=60)

                             GAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGG                                                                 >  SRR4302547.3262784/1‑94 (MQ=60)

                             AAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGG                                                                 <  SRR4302547.533269/94‑1 (MQ=60)

                              AAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGT                                                                <  SRR4302547.3011946/94‑1 (MQ=60)

                              AAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGT                                                                <  SRR4302547.3733873/94‑1 (MQ=60)

                               AATTCTGATCTAGCTAAATGCCTGCAAAAGTGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTG                                                               <  SRR4302547.149025/94‑1 (MQ=60)

                               AATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGTTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTG                                                               <  SRR4302547.4629488/94‑1 (MQ=60)

                               AATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTG                                                               >  SRR4302547.6120151/1‑94 (MQ=60)

                               AATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTG                                                               <  SRR4302547.1074272/94‑1 (MQ=60)

                                ATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGT                                                              >  SRR4302547.5455317/1‑94 (MQ=60)

                                ATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGA                                                              >  SRR4302547.4093348/1‑94 (MQ=60)

                                ATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGA                                                              >  SRR4302547.389751/1‑94 (MQ=60)

                                 TTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGAT                                                             >  SRR4302547.6319457/1‑94 (MQ=60)

                                 TTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTATGGGGTGAT                                                             >  SRR4302547.5426882/1‑94 (MQ=60)

                                  TCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATT                                                            >  SRR4302547.5985961/1‑94 (MQ=60)

                                   CTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTT                                                           >  SRR4302547.4309478/1‑94 (MQ=60)

                                    TGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTT                                                          >  SRR4302547.1155474/1‑94 (MQ=60)

                                       TCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCG                                                       >  SRR4302547.2226474/1‑94 (MQ=60)

                                       TCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCG                                                       >  SRR4302547.2494175/1‑94 (MQ=60)

                                        CTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGT                                                      <  SRR4302547.2597506/94‑1 (MQ=60)

                                        CTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGT                                                      >  SRR4302547.1593005/1‑94 (MQ=60)

                                          AGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAG                                                    <  SRR4302547.6555879/94‑1 (MQ=60)

                                          AGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAG                                                    >  SRR4302547.3248604/1‑94 (MQ=60)

                                            CTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTT                                                  >  SRR4302547.890992/1‑94 (MQ=60)

                                             TAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTA                                                 >  SRR4302547.147149/1‑94 (MQ=60)

                                              AAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTAT                                                <  SRR4302547.4452712/94‑1 (MQ=60)

                                              AAATGCCTGCAAAAGGGTGGTCATTGTCAGGGATATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGCGATTTTTCGTAGTTAT                                                >  SRR4302547.3447180/1‑94 (MQ=60)

                                                ATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTT                                              <  SRR4302547.3147081/94‑1 (MQ=60)

                                                   CCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAA                                           <  SRR4302547.2186057/94‑1 (MQ=60)

                                                   CCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAA                                           <  SRR4302547.1278342/94‑1 (MQ=60)

                                                     TGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATA                                         >  SRR4302547.3545031/1‑94 (MQ=60)

                                                      GCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAA                                        <  SRR4302547.2983593/94‑1 (MQ=60)

                                                       CAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAAT                                       >  SRR4302547.3816412/1‑94 (MQ=60)

                                                       CAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAAT                                       <  SRR4302547.4923146/94‑1 (MQ=60)

                                                        AATAGGGTGGTCATTGTCACAGTTATCTCTCCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATG                                      <  SRR4302547.3558459/94‑1 (MQ=60)

                                                        AAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATG                                      >  SRR4302547.5740051/1‑94 (MQ=60)

                                                        AAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATG                                      >  SRR4302547.5425921/1‑94 (MQ=60)

                                                          AAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAG                                    >  SRR4302547.5224290/1‑94 (MQ=60)

                                                          AAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAG                                    >  SRR4302547.1097716/1‑94 (MQ=60)

                                                          AAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAG                                    >  SRR4302547.2167978/1‑94 (MQ=60)

                                                          AAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAG                                    <  SRR4302547.6269161/94‑1 (MQ=60)

                                                           AGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGA                                   >  SRR4302547.3053645/1‑94 (MQ=60)

                                                           AGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGA                                   <  SRR4302547.1316463/94‑1 (MQ=60)

                                                           AGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGA                                   <  SRR4302547.4066910/94‑1 (MQ=60)

                                                            GGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAA                                  <  SRR4302547.1142590/94‑1 (MQ=60)

                                                             GGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAAC                                 >  SRR4302547.5422064/1‑94 (MQ=60)

                                                             GGTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAAC                                 >  SRR4302547.6114936/1‑94 (MQ=60)

                                                              GTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACA                                <  SRR4302547.5169138/94‑1 (MQ=60)

                                                              GTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACA                                >  SRR4302547.475182/1‑94 (MQ=60)

                                                              GTGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACA                                <  SRR4302547.4973198/94‑1 (MQ=60)

                                                               TGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAG                               <  SRR4302547.4322582/94‑1 (MQ=60)

                                                               TGGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAG                               >  SRR4302547.3163295/1‑94 (MQ=60)

                                                                GGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGG                              >  SRR4302547.1021814/1‑94 (MQ=60)

                                                                GGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGG                              <  SRR4302547.1136857/94‑1 (MQ=60)

                                                                GGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGG                              <  SRR4302547.318454/94‑1 (MQ=60)

                                                                GGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGG                              <  SRR4302547.4767639/94‑1 (MQ=60)

                                                                GGTCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGG                              >  SRR4302547.1438516/1‑94 (MQ=60)

                                                                  TCATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGAC                            >  SRR4302547.6279840/1‑94 (MQ=60)

                                                                   CATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCG                           >  SRR4302547.5684329/1‑94 (MQ=60)

                                                                   CATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCG                           <  SRR4302547.558084/94‑1 (MQ=60)

                                                                   CATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGGGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCG                           >  SRR4302547.1712214/1‑94 (MQ=60)

                                                                    ATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGG                          <  SRR4302547.4077613/94‑1 (MQ=60)

                                                                    ATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGG                          >  SRR4302547.4633070/1‑94 (MQ=60)

                                                                    ATTGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGG                          >  SRR4302547.5232212/1‑94 (MQ=60)

                                                                      TGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAG                        >  SRR4302547.3285119/1‑94 (MQ=60)

                                                                      TGTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAG                        <  SRR4302547.6518912/94‑1 (MQ=60)

                                                                       GTCAGGGTTATCTCTTCCGCTGGCTCCACTGCGGCAAGCTGAGACTGTGGGGCGCTTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGC                       >  SRR4302547.1669858/1‑94 (MQ=60)

                                                                       GTCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGC                       >  SRR4302547.288356/1‑94 (MQ=60)

                                                                        TCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCG                      >  SRR4302547.362068/1‑94 (MQ=60)

                                                                        TCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCG                      <  SRR4302547.407614/94‑1 (MQ=60)

                                                                        TCAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGGGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCG                      >  SRR4302547.385995/1‑94 (MQ=60)

                                                                         CAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGT                     >  SRR4302547.4275878/1‑94 (MQ=60)

                                                                         CAGGGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGT                     <  SRR4302547.5772391/94‑1 (MQ=60)

                                                                            GGTTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAAT                  >  SRR4302547.5666252/1‑94 (MQ=60)

                                                                              TTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTC                >  SRR4302547.6212955/1‑94 (MQ=60)

                                                                              TTATCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTC                >  SRR4302547.4892620/1‑94 (MQ=60)

                                                                                 TCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACA             <  SRR4302547.2901408/94‑1 (MQ=60)

                                                                                 TCTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACA             >  SRR4302547.6423779/1‑94 (MQ=60)

                                                                                  CTCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACAC            >  SRR4302547.4710068/1‑94 (MQ=60)

                                                                                   TCTTCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACA           <  SRR4302547.2959141/94‑1 (MQ=60)

                                                                                      TCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCC        >  SRR4302547.4330072/1‑94 (MQ=60)

                                                                                      TCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCC        <  SRR4302547.4032576/94‑1 (MQ=60)

                                                                                      TCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCC        <  SRR4302547.2684750/94‑1 (MQ=60)

                                                                                      TCCGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCC        <  SRR4302547.5043270/94‑1 (MQ=60)

                                                                                      TCCGCTGCCTCCACTGCGGAAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGCAGCGTAATTCACACAGCC        <  SRR4302547.6111338/94‑1 (MQ=60)

                                                                                        CGCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGG      <  SRR4302547.4905708/94‑1 (MQ=60)

                                                                                         GCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGC     <  SRR4302547.3417991/94‑1 (MQ=60)

                                                                                         GCTGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGC     >  SRR4302547.607312/1‑94 (MQ=60)

                                                                                           TGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCT   >  SRR4302547.13276/1‑94 (MQ=60)

                                                                                           TGCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCT   >  SRR4302547.2885402/1‑94 (MQ=60)

                                                                                            GCCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTT  >  SRR4302547.5869988/1‑94 (MQ=60)

TGAAGAAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTT  >  NC_000913/3896103‑3896288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`