Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,950,557 2 bp→GC coding (451‑452/930 nt) ilvE → branched‑chain amino‑acid aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,5570CG100.0% 877.2 / NA 238R151A (CGA→GCA) ilvEbranched‑chain amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (122/116);  total (122/116)
*NC_0009133,950,5580GC99.6% 860.1 / ‑4.4 238R151A (CGA→GCA) ilvEbranched‑chain amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (122/115);  total (122/116)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

Only 100 of 246 total aligned reads displayed.

ATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGT  >  NC_000913/3950470‑3950643
                                                                                       ||                                                                                     
aTCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCa                                                                                   >  1:4269391/1‑93 (MQ=255)
     tgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAacac                                                                              <  2:255843/93‑1 (MQ=255)
     tgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAacac                                                                              >  1:3167188/1‑93 (MQ=255)
     tgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAacac                                                                              >  1:2613150/1‑93 (MQ=255)
       ctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCa                                                                            <  1:5316778/93‑1 (MQ=255)
       ctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCa                                                                            <  1:2689677/93‑1 (MQ=255)
        tttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt                                                                           <  1:1122931/93‑1 (MQ=255)
        tttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt                                                                           >  1:769779/1‑93 (MQ=255)
         ttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATc                                                                          >  2:5152491/1‑93 (MQ=255)
         ttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATc                                                                          >  1:3311079/1‑93 (MQ=255)
          tCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc                                                                         <  2:1012152/93‑1 (MQ=255)
           cccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATccc                                                                        <  1:687142/93‑1 (MQ=255)
            ccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCg                                                                       <  1:5196413/93‑1 (MQ=255)
            ccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCg                                                                       <  1:3731733/93‑1 (MQ=255)
             cGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGa                                                                      <  2:712452/93‑1 (MQ=255)
             cGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGa                                                                      <  2:1863150/93‑1 (MQ=255)
              gTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc                                                                     >  2:6544328/1‑93 (MQ=255)
                ggggAGCGTATCTGGGCGCAGAAGCGCTGGATCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcgg                                                                   <  2:2196270/93‑1 (MQ=255)
                ggggAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcgg                                                                   >  1:2118112/1‑93 (MQ=255)
                 gggAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggc                                                                  >  1:5677638/1‑93 (MQ=255)
                   gAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggcgg                                                                <  2:1645051/93‑1 (MQ=255)
                    aGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACggcggc                                                               <  2:6361663/93‑1 (MQ=255)
                     gCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa                                                              >  2:2738938/1‑93 (MQ=255)
                     gCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa                                                              >  1:1564100/1‑93 (MQ=255)
                      cGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaa                                                             <  2:3954823/93‑1 (MQ=255)
                      cGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaa                                                             <  2:119645/93‑1 (MQ=255)
                       gTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaa                                                            <  1:1318067/93‑1 (MQ=255)
                       gTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaa                                                            >  2:1809077/1‑93 (MQ=255)
                        tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa                                                           >  1:2129932/1‑93 (MQ=255)
                        tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa                                                           <  2:4533146/93‑1 (MQ=255)
                        tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa                                                           <  1:5166463/93‑1 (MQ=255)
                          tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc                                                         >  1:3791337/1‑93 (MQ=255)
                          tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc                                                         >  2:2808129/1‑93 (MQ=255)
                          tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc                                                         >  2:3605003/1‑93 (MQ=255)
                           cTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGcc                                                        <  2:3638727/93‑1 (MQ=255)
                           cTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGcc                                                        >  1:2461642/1‑93 (MQ=255)
                            tGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCg                                                       <  2:257925/93‑1 (MQ=255)
                            tGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCg                                                       <  1:4542877/93‑1 (MQ=255)
                            tGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCg                                                       >  1:1671534/1‑93 (MQ=255)
                              ggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggt                                                     <  1:5967924/93‑1 (MQ=255)
                               gcgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtg                                                    <  2:4941801/93‑1 (MQ=255)
                               gcgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtg                                                    >  2:264107/1‑93 (MQ=255)
                                  cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTa                                                 >  1:3712017/1‑93 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc                                               >  2:3700880/1‑93 (MQ=255)
                                      aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTa                                             >  2:2070787/1‑93 (MQ=255)
                                       gcgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAc                                            >  2:2538719/1‑93 (MQ=255)
                                       gcgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAc                                            >  2:1051915/1‑93 (MQ=255)
                                       gcgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAc                                            >  1:4059670/1‑93 (MQ=255)
                                        cgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAcc                                           >  2:1975086/1‑93 (MQ=255)
                                        cgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAcc                                           <  2:234022/93‑1 (MQ=255)
                                        cgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAcc                                           >  1:3348155/1‑93 (MQ=255)
                                        cgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAcc                                           >  2:5364172/1‑93 (MQ=255)
                                         gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACct                                          >  1:5534372/1‑93 (MQ=255)
                                           tGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctct                                        <  2:1375430/93‑1 (MQ=255)
                                            ggAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctc                                       <  1:4860714/93‑1 (MQ=255)
                                            ggAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctc                                       >  1:4385019/1‑93 (MQ=255)
                                            ggAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctc                                       <  1:2377306/93‑1 (MQ=255)
                                              aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCtt                                     >  1:1136818/1‑93 (MQ=255)
                                               gCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTc                                    >  1:5796981/1‑93 (MQ=255)
                                               gCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTc                                    >  1:6286548/1‑93 (MQ=255)
                                                cAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTcc                                   <  2:2417074/93‑1 (MQ=255)
                                                 aGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTccc                                  <  2:44307/93‑1 (MQ=255)
                                                 aGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTccc                                  >  2:1585681/1‑93 (MQ=255)
                                                 aGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTccc                                  >  1:2878425/1‑93 (MQ=255)
                                                  ggggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCct                                 <  2:3428014/93‑1 (MQ=255)
                                                  ggggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCct                                 <  1:5741832/93‑1 (MQ=255)
                                                  ggggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCct                                 <  1:895325/93‑1 (MQ=255)
                                                  ggggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCct                                 <  2:2510226/93‑1 (MQ=255)
                                                    ggATCGATGCGATGGTTTCCTCCTGGACCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgc                               <  2:3889001/93‑1 (MQ=255)
                                                    ggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgc                               <  1:5750324/93‑1 (MQ=255)
                                                     gATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgct                              <  2:488233/93‑1 (MQ=255)
                                                       acgatgcgatgGTTGCCTCCTGGAACCGCGCACCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGCAACTACCTCTCTTCCCTGCtgg                            <  1:1391953/92‑1 (MQ=255)
                                                          atgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtgg                         >  2:1925069/1‑93 (MQ=255)
                                                          atgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtgg                         >  1:2226513/1‑93 (MQ=255)
                                                              gatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAg                     <  1:3217178/93‑1 (MQ=255)
                                                               atgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGc                    >  2:5127647/1‑93 (MQ=255)
                                                               atgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGc                    >  2:4329068/1‑93 (MQ=255)
                                                                tgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCg                   >  1:2959897/1‑93 (MQ=255)
                                                                tgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCg                   >  2:5531223/1‑93 (MQ=255)
                                                                 ggTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGa                  <  2:6208099/93‑1 (MQ=255)
                                                                     tcctccTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcg              <  2:3135188/93‑1 (MQ=255)
                                                                      cctcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcgc             <  2:6056019/93‑1 (MQ=255)
                                                                       ctcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcgcg            >  1:3895858/1‑93 (MQ=255)
                                                                        ccctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcgcgc           <  2:1366986/92‑1 (MQ=255)
                                                                         cctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgcc          >  1:1003140/1‑93 (MQ=255)
                                                                         cctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgcc          >  1:2069194/1‑93 (MQ=255)
                                                                          ctGGAACCGCGCAGCACCAAACACCATCCCGACGGAGGCGAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccg         >  1:1740146/1‑93 (MQ=255)
                                                                           tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccgc        <  1:5640742/93‑1 (MQ=255)
                                                                            ggAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCgccgcc       >  2:1902781/1‑93 (MQ=255)
                                                                            ggAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCgccgcc       >  2:818867/1‑93 (MQ=255)
                                                                             gAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCa      >  1:1979017/1‑93 (MQ=255)
                                                                             gAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCa      >  2:3534124/1‑93 (MQ=255)
                                                                              aaCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCAc     >  2:5180412/1‑93 (MQ=255)
                                                                              aaCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCAc     >  2:5683568/1‑93 (MQ=255)
                                                                              aaCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCAc     >  1:6442259/1‑93 (MQ=255)
                                                                               aCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACg    >  1:341822/1‑93 (MQ=255)
                                                                               aCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACg    >  2:4265795/1‑93 (MQ=255)
                                                                                ccGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACgg   <  2:6479559/93‑1 (MQ=255)
                                                                                ccGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACgg   >  1:938687/1‑93 (MQ=255)
                                                                                 cgcgCAGCACCAAACAGCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGt  >  1:1546855/1‑93 (MQ=255)
                                                                                       ||                                                                                     
ATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGT  >  NC_000913/3950470‑3950643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  >  NC_000913/3950469‑3950644
                                                                                        |                                                                                       
TATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCA                                                                                    >  SRR4302547.4356999/1‑94 (MQ=60)
  TCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAA                                                                                  >  SRR4302547.1221343/1‑94 (MQ=60)
   CGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGCGCAGGGGATCGATGCGATAGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                 >  SRR4302547.297121/1‑94 (MQ=60)
    GCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACA                                                                                >  SRR4302547.3377446/1‑94 (MQ=60)
     CTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAC                                                                               >  SRR4302547.3230208/1‑94 (MQ=60)
     CTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAC                                                                               >  SRR4302547.3920439/1‑94 (MQ=60)
     CTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAC                                                                               >  SRR4302547.2665808/1‑94 (MQ=60)
      TGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCAGCAAACACC                                                                              >  SRR4302547.3843656/1‑94 (MQ=60)
        CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                            >  SRR4302547.784326/1‑94 (MQ=60)
        CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                            >  SRR4302547.2919325/1‑94 (MQ=60)
        CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                            <  SRR4302547.2743669/94‑1 (MQ=60)
        CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                            >  SRR4302547.2360489/1‑94 (MQ=60)
        CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                            <  SRR4302547.5426418/94‑1 (MQ=60)
         TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATC                                                                           <  SRR4302547.5772128/94‑1 (MQ=60)
         TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATC                                                                           >  SRR4302547.3377840/1‑94 (MQ=60)
         TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATC                                                                           <  SRR4302547.1145580/94‑1 (MQ=60)
            CCGGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                        <  SRR4302547.329059/94‑1 (MQ=60)
            CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                        >  SRR4302547.6179309/1‑94 (MQ=60)
            CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                        >  SRR4302547.663266/1‑94 (MQ=60)
            CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                        <  SRR4302547.699823/94‑1 (MQ=60)
             CCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGA                                                                       <  SRR4302547.5303355/94‑1 (MQ=60)
             CCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGA                                                                       <  SRR4302547.3807928/94‑1 (MQ=60)
             CCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGA                                                                       >  SRR4302547.1846027/1‑94 (MQ=60)
              CGTGGGGAGCTTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAC                                                                      <  SRR4302547.1513447/94‑1 (MQ=60)
              CGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAC                                                                      >  SRR4302547.2598267/1‑94 (MQ=60)
               GTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACG                                                                     >  SRR4302547.3259232/1‑94 (MQ=60)
                TGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGG                                                                    >  SRR4302547.2160328/1‑94 (MQ=60)
                 GGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGC                                                                   >  SRR4302547.5794873/1‑94 (MQ=60)
                 GGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGC                                                                   >  SRR4302547.5333338/1‑94 (MQ=60)
                     AGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCA                                                               >  SRR4302547.1595254/1‑94 (MQ=60)
                       CGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAA                                                             >  SRR4302547.348148/1‑94 (MQ=60)
                        GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAA                                                            <  SRR4302547.2168906/94‑1 (MQ=60)
                        GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAA                                                            >  SRR4302547.2172372/1‑94 (MQ=60)
                        GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAA                                                            >  SRR4302547.150193/1‑94 (MQ=60)
                        GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAA                                                            <  SRR4302547.1344557/94‑1 (MQ=60)
                         TATCTGGGCGCAGAAGCGCTGGAGCAGTGGATCGATGGGATGGTTTCCTCCTGGAACCGCGCAGCACCAACCACCATCCCGACGGCGGCAAAAG                                                           <  SRR4302547.4779712/94‑1 (MQ=60)
                         TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                           <  SRR4302547.5965681/94‑1 (MQ=60)
                         TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                           <  SRR4302547.5272834/94‑1 (MQ=60)
                         TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                           >  SRR4302547.5560343/1‑94 (MQ=60)
                          ATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGC                                                          >  SRR4302547.3868639/1‑94 (MQ=60)
                          ATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGC                                                          <  SRR4302547.5801358/94‑1 (MQ=60)
                           TCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCC                                                         >  SRR4302547.2511398/1‑94 (MQ=60)
                           TCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCC                                                         <  SRR4302547.5027184/94‑1 (MQ=60)
                           TCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCC                                                         >  SRR4302547.1390176/1‑94 (MQ=60)
                            CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                        <  SRR4302547.1679148/94‑1 (MQ=60)
                            CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                        <  SRR4302547.2130141/94‑1 (MQ=60)
                            CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                        >  SRR4302547.1704464/1‑94 (MQ=60)
                             TGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGG                                                       <  SRR4302547.4636802/94‑1 (MQ=60)
                              GGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGT                                                      <  SRR4302547.4544040/94‑1 (MQ=60)
                               GGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTG                                                     <  SRR4302547.6091707/94‑1 (MQ=60)
                               GGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTG                                                     <  SRR4302547.963921/94‑1 (MQ=60)
                                GCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGG                                                    >  SRR4302547.5841565/1‑94 (MQ=60)
                                 CGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGT                                                   >  SRR4302547.1156395/1‑94 (MQ=60)
                                 CGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGT                                                   >  SRR4302547.2654630/1‑94 (MQ=60)
                                  GCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTA                                                  >  SRR4302547.945754/1‑94 (MQ=60)
                                  GCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTA                                                  >  SRR4302547.3787786/1‑94 (MQ=60)
                                  GCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTA                                                  <  SRR4302547.1671983/94‑1 (MQ=60)
                                   CAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAA                                                 <  SRR4302547.4409268/94‑1 (MQ=60)
                                     GAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACT                                               <  SRR4302547.2067313/94‑1 (MQ=60)
                                       AGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAC                                             >  SRR4302547.4142120/1‑94 (MQ=60)
                                       AGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAC                                             >  SRR4302547.5694370/1‑94 (MQ=60)
                                        GCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACC                                            <  SRR4302547.3389789/94‑1 (MQ=60)
                                        GCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACC                                            >  SRR4302547.3416032/1‑94 (MQ=60)
                                         CGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCT                                           >  SRR4302547.5648591/1‑94 (MQ=60)
                                          GCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTC                                          >  SRR4302547.2377863/1‑94 (MQ=60)
                                           CTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCT                                         >  SRR4302547.4089713/1‑94 (MQ=60)
                                           CTGGAGCAGGGGAGCGATGCGATGGTTTCCTCCGGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGTAAAAGCCGGTGGTAACTTCCTCT                                         <  SRR4302547.1564671/94‑1 (MQ=60)
                                            TGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTC                                        >  SRR4302547.4475235/1‑94 (MQ=60)
                                            GGGAGCAGGGGATCGATGCGATGGTGTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTC                                        <  SRR4302547.1036762/94‑1 (MQ=60)
                                             GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                       <  SRR4302547.4961441/94‑1 (MQ=60)
                                             GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                       >  SRR4302547.2127972/1‑94 (MQ=60)
                                             GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCA                                       <  SRR4302547.2425468/94‑1 (MQ=60)
                                              GAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTT                                      >  SRR4302547.1159775/1‑94 (MQ=60)
                                               AGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTC                                     >  SRR4302547.6417164/1‑94 (MQ=60)
                                               AGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTC                                     >  SRR4302547.5916893/1‑94 (MQ=60)
                                                 GAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                   <  SRR4302547.702399/94‑1 (MQ=60)
                                                 CAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                   >  SRR4302547.2679303/1‑94 (MQ=60)
                                                 CAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                   >  SRR4302547.2935884/1‑94 (MQ=60)
                                                 CAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                   >  SRR4302547.5638630/1‑94 (MQ=60)
                                                  AGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCT                                  >  SRR4302547.2858166/1‑94 (MQ=60)
                                                   GGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTG                                 <  SRR4302547.126020/94‑1 (MQ=60)
                                                   GGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTG                                 <  SRR4302547.912547/94‑1 (MQ=60)
                                                   GGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTG                                 <  SRR4302547.5860485/94‑1 (MQ=60)
                                                     GGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCT                               <  SRR4302547.4369701/94‑1 (MQ=60)
                                                     GGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCT                               <  SRR4302547.5869187/94‑1 (MQ=60)
                                                      GATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTG                              >  SRR4302547.378388/1‑94 (MQ=60)
                                                       gaccaatcngTGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGG                             <  SRR4302547.6206762/84‑1 (MQ=60)
                                                       ATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGG                             <  SRR4302547.4935854/94‑1 (MQ=60)
                                                       ATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGG                             <  SRR4302547.3581328/94‑1 (MQ=60)
                                                        ACGATGCGATGGTTGCCTCCTGGAACCGCGCACCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGCAACTACCTCTCTTCCCTGCTGGT                            <  SRR4302547.1419810/94‑1 (MQ=60)
                                                         CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                           >  SRR4302547.4158576/1‑94 (MQ=60)
                                                          GATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGG                          >  SRR4302547.2271383/1‑94 (MQ=60)
                                                          GATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAACTCTCTTCCCTGCTGGTGG                          >  SRR4302547.2478446/1‑94 (MQ=60)
                                                             GCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTA                       <  SRR4302547.6484527/94‑1 (MQ=60)
                                                               GATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGC                     <  SRR4302547.3281349/94‑1 (MQ=60)
                                                               GATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGC                     >  SRR4302547.4006463/1‑94 (MQ=60)
                                                               GATGGTTCCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGC                     <  SRR4302547.5878855/94‑1 (MQ=60)
                                                                ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                    <  SRR4302547.1766236/94‑1 (MQ=60)
                                                                ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                    >  SRR4302547.3018807/1‑94 (MQ=60)
                                                                ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                    <  SRR4302547.3024113/94‑1 (MQ=60)
                                                                  GGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAA                  <  SRR4302547.6620702/94‑1 (MQ=60)
                                                                   GTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAG                 >  SRR4302547.6209652/1‑94 (MQ=60)
                                                                    TTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGC                >  SRR4302547.585049/1‑94 (MQ=60)
                                                                     TGCCTCCTGGAACCGCGCAGCACCAAGCACCATCCCGACGGCGGCAAAAGCCGTTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCG               <  SRR4302547.5882321/94‑1 (MQ=60)
                                                                      TCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGC              <  SRR4302547.5287634/94‑1 (MQ=60)
                                                                      TCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGC              <  SRR4302547.1584467/94‑1 (MQ=60)
                                                                      TCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGC              >  SRR4302547.5275679/1‑94 (MQ=60)
                                                                       CCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCG             >  SRR4302547.3975073/1‑94 (MQ=60)
                                                                        CTCCTGGAACCGGGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTATCTTCCCTGCTGGTGGGTAGCGAAGCGCGC            >  SRR4302547.6613825/1‑94 (MQ=60)
                                                                        CTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGC            >  SRR4302547.184490/1‑94 (MQ=60)
                                                                        CTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGC            <  SRR4302547.2200184/94‑1 (MQ=60)
                                                                         TCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC           <  SRR4302547.831608/94‑1 (MQ=60)
                                                                         TCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC           >  SRR4302547.1022710/1‑94 (MQ=60)
                                                                         TCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC           >  SRR4302547.2110483/1‑94 (MQ=60)
                                                                         TCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC           <  SRR4302547.3457790/94‑1 (MQ=60)
                                                                         TCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAACGAAGCcggcc           >  SRR4302547.1265927/1‑89 (MQ=60)
                                                                          CCTGGAACCGCGCAGCACCAAACACCATCCCGACGGAGGCGAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCG          >  SRR4302547.1774373/1‑94 (MQ=60)
                                                                            TGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCC        <  SRR4302547.5757183/94‑1 (MQ=60)
                                                                             GGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCA       >  SRR4302547.2018299/1‑94 (MQ=60)
                                                                              GAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCAC      >  SRR4302547.6576511/1‑94 (MQ=60)
                                                                               AACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACG     <  SRR4302547.884773/94‑1 (MQ=60)
                                                                               AACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACG     >  SRR4302547.348170/1‑94 (MQ=60)
                                                                                ACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGG    >  SRR4302547.956865/1‑94 (MQ=60)
                                                                                 CCGCGCAGCACCAAACAGCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGT   >  SRR4302547.1577649/1‑94 (MQ=60)
                                                                                 CCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGT   >  SRR4302547.186255/1‑94 (MQ=60)
                                                                                 CCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGCGGGTAGCGAAGCGCGCCGCCACGGT   >  SRR4302547.3045495/1‑94 (MQ=60)
                                                                                  CGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  <  SRR4302547.3861780/94‑1 (MQ=60)
                                                                                        |                                                                                       
TATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  >  NC_000913/3950469‑3950644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑