Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,971,075 2 bp→GC coding (942‑943/1068 nt) rffG → dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,971,0750CG100.0% 880.2 / NA 240C314W (TGC→TGG) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (122/118);  total (122/118)
*NC_0009133,971,0760GC99.6% 871.5 / ‑5.0 240V315L (GTG→CTG) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (122/117);  minor base T (0/1);  total (122/118)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 245 total aligned reads displayed.

TGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGA  >  NC_000913/3970988‑3971161
                                                                                       ||                                                                                     
tGACCTGATCTCCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGcc                                                                                   <  1:106858/93‑1 (MQ=255)
tGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGcc                                                                                   >  1:3831649/1‑93 (MQ=255)
tGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGcc                                                                                   >  1:26512/1‑93 (MQ=255)
     tGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAgg                                                                              <  2:233279/93‑1 (MQ=255)
     tGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAgg                                                                              <  1:857242/93‑1 (MQ=255)
     tGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAgg                                                                              >  1:57972/1‑93 (MQ=255)
      gATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGa                                                                             >  1:287553/1‑93 (MQ=255)
      gATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGa                                                                             >  2:5786682/1‑93 (MQ=255)
         cACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAc                                                                          >  2:3811120/1‑93 (MQ=255)
         cACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAc                                                                          <  1:4635764/93‑1 (MQ=255)
          aCCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAGcc                                                                         <  2:252776/93‑1 (MQ=255)
          aCCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAcc                                                                         >  2:6313498/1‑93 (MQ=255)
           ccTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTTCCGCAGGAAACCt                                                                        <  2:6028723/93‑1 (MQ=255)
           ccTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCt                                                                        >  2:257493/1‑93 (MQ=255)
           ccTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCt                                                                        >  1:188136/1‑93 (MQ=255)
           ccTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCt                                                                        >  1:4955056/1‑93 (MQ=255)
            cTTTGTCGCTGATCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCtt                                                                       <  1:62965/93‑1 (MQ=39)
             tttGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCttt                                                                      >  2:4399100/1‑93 (MQ=255)
              ttGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTg                                                                     <  1:4785707/93‑1 (MQ=255)
                gTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGaa                                                                   >  1:5906229/1‑93 (MQ=255)
                 tCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGaaa                                                                  <  1:4438395/93‑1 (MQ=255)
                 tCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCGTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGaaa                                                                  <  1:2287401/93‑1 (MQ=255)
                    cTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTg                                                               >  2:1343903/1‑93 (MQ=255)
                    cTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTg                                                               >  2:770629/1‑93 (MQ=255)
                    cTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTg                                                               <  1:5852948/93‑1 (MQ=255)
                     tGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTgg                                                              >  1:4399092/1‑93 (MQ=255)
                      gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa                                                             <  1:791657/93‑1 (MQ=255)
                      gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa                                                             <  2:4865106/93‑1 (MQ=255)
                       aCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGaa                                                            <  1:3749151/93‑1 (MQ=255)
                       aCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGaa                                                            >  2:1253378/1‑93 (MQ=255)
                       aCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGaa                                                            >  2:6461840/1‑93 (MQ=255)
                       aCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGaa                                                            <  1:3361383/93‑1 (MQ=255)
                        ccGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAt                                                           >  2:2667031/1‑93 (MQ=255)
                         cGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATg                                                          <  1:4246682/93‑1 (MQ=255)
                          gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGc                                                         <  1:3519485/93‑1 (MQ=255)
                          gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGc                                                         >  1:4114957/1‑93 (MQ=255)
                           tCCGGGGCATGATCTGCGTTATGCCATTGATGTTTCGAAAATTGCCCGTTAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCg                                                        >  2:842071/1‑93 (MQ=255)
                           tCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCg                                                        <  1:980031/93‑1 (MQ=255)
                            ccGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGt                                                       <  1:3567733/93‑1 (MQ=255)
                             cGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTa                                                      <  1:3645110/93‑1 (MQ=255)
                              ggggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTaa                                                     <  1:3982635/93‑1 (MQ=255)
                                ggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTaaaa                                                   <  1:4808767/93‑1 (MQ=255)
                                ggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTaaaa                                                   <  2:5159007/93‑1 (MQ=255)
                                ggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTaaaa                                                   >  2:4410366/1‑93 (MQ=255)
                                 gCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAAc                                                  <  1:6520128/93‑1 (MQ=255)
                                 gCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAAc                                                  <  1:257433/93‑1 (MQ=255)
                                   aTGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACgg                                                <  1:6126039/93‑1 (MQ=255)
                                    tGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGt                                               >  2:4366949/1‑93 (MQ=255)
                                    tGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGt                                               >  1:2954181/1‑93 (MQ=255)
                                    tGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGt                                               <  1:5063707/93‑1 (MQ=255)
                                     gATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGtt                                              >  2:1040299/1‑93 (MQ=255)
                                      aTCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTc                                             <  1:6347484/93‑1 (MQ=255)
                                      aTCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTc                                             <  1:6160134/93‑1 (MQ=255)
                                      aTCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTc                                             <  1:3704010/93‑1 (MQ=255)
                                       tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCa                                            <  2:8131/93‑1 (MQ=255)
                                       tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCa                                            >  1:6513868/1‑93 (MQ=255)
                                       tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCa                                            >  2:317307/1‑93 (MQ=255)
                                       tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCa                                            >  2:5625305/1‑93 (MQ=255)
                                        cTGCGGTATGCCATTGATGCTTGGAAAATTTCCCGTGAACTTGGCTGGCTGCCGCAGGGAACCTGTGGAAGGGGCATGCGTAAAACGGTTCAg                                           >  1:2374769/1‑93 (MQ=255)
                                         tGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGt                                          <  2:3388500/93‑1 (MQ=255)
                                          gCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTg                                         >  2:3116381/1‑93 (MQ=255)
                                           cGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTgg                                        <  2:1811478/93‑1 (MQ=255)
                                            gTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGt                                       <  2:867185/93‑1 (MQ=255)
                                            gTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGt                                       <  1:1321027/93‑1 (MQ=255)
                                            gTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGt                                       >  1:5399674/1‑93 (MQ=255)
                                             ttATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTa                                      <  1:3452950/93‑1 (MQ=255)
                                             ttATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTa                                      <  1:3114394/93‑1 (MQ=255)
                                               aTGCCATTTATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATc                                    <  1:3304596/93‑1 (MQ=255)
                                               aTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATc                                    <  2:4999853/93‑1 (MQ=255)
                                               aTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATc                                    <  2:1714504/93‑1 (MQ=255)
                                               aTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATc                                    <  2:6619399/93‑1 (MQ=255)
                                                tGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCt                                   <  1:4069631/93‑1 (MQ=255)
                                                tGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCt                                   >  1:3182018/1‑93 (MQ=255)
                                                  ccATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTgg                                 >  2:4458779/1‑93 (MQ=255)
                                                     ttGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTa                              >  2:2202427/1‑93 (MQ=255)
                                                     ttGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTa                              >  1:2004350/1‑93 (MQ=255)
                                                      tGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTaa                             >  2:5088518/1‑93 (MQ=255)
                                                       gATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAAt                            <  1:160683/93‑1 (MQ=255)
                                                         tGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGa                          <  2:42602/93‑1 (MQ=255)
                                                         tGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGa                          >  2:4844592/1‑93 (MQ=255)
                                                         tGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGa                          <  2:1876245/93‑1 (MQ=255)
                                                            ttCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAg                       >  2:3840932/1‑93 (MQ=255)
                                                             tCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGc                      <  1:5568554/93‑1 (MQ=255)
                                                             tCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGc                      >  1:3272951/1‑93 (MQ=255)
                                                              cGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAATCCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTACTGAAAGCt                     <  1:6492892/93‑1 (MQ=255)
                                                               gAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtg                    <  2:6147311/93‑1 (MQ=255)
                                                                aaaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAGAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtgg                   >  1:2503402/1‑93 (MQ=255)
                                                                aaaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtgg                   <  1:119858/93‑1 (MQ=255)
                                                                aaaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtgg                   <  2:272757/93‑1 (MQ=255)
                                                                     tGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGaa              >  1:1685753/1‑93 (MQ=255)
                                                                     tGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGaa              <  2:1960003/93‑1 (MQ=255)
                                                                       cccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGc            <  2:4082232/93‑1 (MQ=255)
                                                                       cccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGc            >  2:6441258/1‑93 (MQ=255)
                                                                         cGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAg          <  1:5502260/93‑1 (MQ=255)
                                                                         cGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAg          >  2:2913561/1‑93 (MQ=255)
                                                                           tGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGt        <  2:4576412/93‑1 (MQ=255)
                                                                             aaCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGc      <  1:4352440/93‑1 (MQ=255)
                                                                               cTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAg    <  2:2967917/93‑1 (MQ=255)
                                                                                ttGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAgg   <  1:5118734/93‑1 (MQ=255)
                                                                                 tggctggcTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGa  >  2:1649631/1‑93 (MQ=255)
                                                                                       ||                                                                                     
TGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGA  >  NC_000913/3970988‑3971161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCG‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGA  >  NC_000913/3970982‑3971161
                                                                                             |                                                                                         
CTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGG                                                                                           >  SRR4302547.3981559/1‑94 (MQ=60)
CTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGG                                                                                           <  SRR4302547.651647/94‑1 (MQ=60)
CTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGG                                                                                           >  SRR4302547.4196016/1‑94 (MQ=60)
     GTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCC                                                                                   >  SRR4302547.26994/1‑94 (MQ=60)
     GTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCC                                                                                   >  SRR4302547.3909702/1‑94 (MQ=60)
      TGACCTGATCTCCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCG                                                                                  <  SRR4302547.108810/94‑1 (MQ=60)
      TGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCG                                                                                  <  SRR4302547.1611531/94‑1 (MQ=60)
      TGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCG                                                                                  >  SRR4302547.2726728/1‑94 (MQ=60)
        ACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCA                                                                                <  SRR4302547.4092668/94‑1 (MQ=60)
         CCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAG                                                                               <  SRR4302547.3292594/94‑1 (MQ=60)
          CTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGG                                                                              >  SRR4302547.59070/1‑94 (MQ=60)
          CTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGG                                                                              <  SRR4302547.4510569/94‑1 (MQ=60)
           TGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGA                                                                             >  SRR4302547.5613490/1‑94 (MQ=60)
           TGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGA                                                                             >  SRR4302547.292894/1‑94 (MQ=60)
           TGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGA                                                                             <  SRR4302547.873622/94‑1 (MQ=60)
           TGATCACCTTTGTCGCGGTGCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGA                                                                             <  SRR4302547.1877979/94‑1 (MQ=60)
               CACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACC                                                                         <  SRR4302547.6654729/94‑1 (MQ=60)
               CACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACC                                                                         <  SRR4302547.4731786/94‑1 (MQ=60)
                ACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCT                                                                        >  SRR4302547.191680/1‑94 (MQ=60)
                ACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCT                                                                        >  SRR4302547.5057618/1‑94 (MQ=60)
                 CCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTT                                                                       >  SRR4302547.3452910/1‑94 (MQ=60)
                 CCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTT                                                                       >  SRR4302547.4915854/1‑94 (MQ=60)
                  CTTTGTCGCTGATCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTT                                                                      <  SRR4302547.64149/94‑1 (MQ=60)
                    TTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGA                                                                    <  SRR4302547.5204959/94‑1 (MQ=60)
                    TTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGA                                                                    <  SRR4302547.4884834/94‑1 (MQ=60)
                    TTGTCGCGGACCGGCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGT‑‑‑TGCCGCAGGAAACCTTTGA                                                                    <  SRR4302547.4211863/94‑1 (MQ=60)
                     TGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAA                                                                   >  SRR4302547.6028550/1‑94 (MQ=60)
                      GTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAA                                                                  >  SRR4302547.2388355/1‑94 (MQ=60)
                       TCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAG                                                                 <  SRR4302547.2866839/94‑1 (MQ=60)
                       TCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAG                                                                 <  SRR4302547.4530161/94‑1 (MQ=60)
                       TCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCGTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAG                                                                 <  SRR4302547.2333711/94‑1 (MQ=60)
                        CGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGT                                                                <  SRR4302547.3199623/94‑1 (MQ=60)
                          CTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGG                                                              >  SRR4302547.5171941/1‑94 (MQ=60)
                          CTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGG                                                              >  SRR4302547.4489669/1‑94 (MQ=60)
                          CTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGG                                                              <  SRR4302547.5974037/94‑1 (MQ=60)
                           TGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGA                                                             <  SRR4302547.2652965/94‑1 (MQ=60)
                           TGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGA                                                             >  SRR4302547.5378328/1‑94 (MQ=60)
                            GACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAA                                                            <  SRR4302547.806637/94‑1 (MQ=60)
                            GACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAA                                                            >  SRR4302547.966938/1‑94 (MQ=60)
                             ACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAAT                                                           <  SRR4302547.3825678/94‑1 (MQ=60)
                             ACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAAT                                                           <  SRR4302547.3429611/94‑1 (MQ=60)
                              CCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATG                                                          >  SRR4302547.3721681/1‑94 (MQ=60)
                              CCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATG                                                          >  SRR4302547.838587/1‑94 (MQ=60)
                               CGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGC                                                         >  SRR4302547.4198585/1‑94 (MQ=60)
                               CGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGC                                                         >  SRR4302547.3370160/1‑94 (MQ=60)
                               CGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGC                                                         <  SRR4302547.3396719/94‑1 (MQ=60)
                               CGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGC                                                         <  SRR4302547.2453490/94‑1 (MQ=60)
                               CGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGA                                                         <  SRR4302547.4333548/94‑1 (MQ=60)
                                GTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCG                                                        <  SRR4302547.3591328/94‑1 (MQ=60)
                                 TCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGT                                                       <  SRR4302547.999100/94‑1 (MQ=60)
                                  CCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTA                                                      <  SRR4302547.3640522/94‑1 (MQ=60)
                                  CCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTA                                                      <  SRR4302547.3648641/94‑1 (MQ=60)
                                   CGGGGCATGGTCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAA                                                     >  SRR4302547.6594392/1‑94 (MQ=60)
                                   CGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAA                                                     <  SRR4302547.3719461/94‑1 (MQ=60)
                                   CGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAA                                                     <  SRR4302547.3826724/94‑1 (MQ=60)
                                    GGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAA                                                    >  SRR4302547.557717/1‑94 (MQ=60)
                                    GGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAA                                                    <  SRR4302547.5650098/94‑1 (MQ=60)
                                    GGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAA                                                    <  SRR4302547.4063621/94‑1 (MQ=60)
                                     GGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAA                                                   >  SRR4302547.6219025/1‑94 (MQ=60)
                                     GGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAAGTGCCCGTGAACTTGTCTGGC‑‑‑GGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAA                                                   <  SRR4302547.1716777/94‑1 (MQ=60)
                                      GGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAAC                                                  <  SRR4302547.4908440/94‑1 (MQ=60)
                                       GCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACG                                                 <  SRR4302547.5940502/94‑1 (MQ=60)
                                       GCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACG                                                 <  SRR4302547.6655872/94‑1 (MQ=60)
                                       GCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACG                                                 <  SRR4302547.262254/94‑1 (MQ=60)
                                       GCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACG                                                 <  SRR4302547.6373088/94‑1 (MQ=60)
                                         ATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGT                                               >  SRR4302547.3861793/1‑94 (MQ=60)
                                         ATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGT                                               <  SRR4302547.6253117/94‑1 (MQ=60)
                                         ATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGT                                               >  SRR4302547.3012982/1‑94 (MQ=60)
                                         ATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGT                                               <  SRR4302547.4896643/94‑1 (MQ=60)
                                         ATGATCTGCGTTATGCCATTGATGCTTCGAAAATCGCCCGTGATCTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGT                                               <  SRR4302547.886121/94‑1 (MQ=60)
                                          TGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTT                                              <  SRR4302547.6287038/94‑1 (MQ=60)
                                          TGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTT                                              <  SRR4302547.5168206/94‑1 (MQ=60)
                                           GATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTCAAACGGTTC                                             <  SRR4302547.6603675/94‑1 (MQ=60)
                                           GATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTC                                             >  SRR4302547.4381299/1‑94 (MQ=60)
                                           GATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTC                                             >  SRR4302547.1095902/1‑94 (MQ=60)
                                            ATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCA                                            >  SRR4302547.6649469/1‑94 (MQ=60)
                                            ATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCA                                            <  SRR4302547.4487070/94‑1 (MQ=60)
                                            ATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCA                                            >  SRR4302547.2479501/1‑94 (MQ=60)
                                            ATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCA                                            <  SRR4302547.6479724/94‑1 (MQ=60)
                                            ATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCA                                            <  SRR4302547.3779616/94‑1 (MQ=60)
                                            ATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCA                                            <  SRR4302547.6287975/94‑1 (MQ=60)
                                             TCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAG                                           >  SRR4302547.3068356/1‑94 (MQ=60)
                                              CTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGT                                          >  SRR4302547.4178013/1‑94 (MQ=60)
                                               TGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTG                                         <  SRR4302547.4804720/94‑1 (MQ=60)
                                               TGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTG                                         <  SRR4302547.3773739/94‑1 (MQ=60)
                                               TGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTG                                         >  SRR4302547.2011515/1‑94 (MQ=60)
                                                GCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGG                                        >  SRR4302547.305226/1‑94 (MQ=60)
                                                 CGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGT                                       >  SRR4302547.5511267/1‑94 (MQ=60)
                                                 CGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGT                                       >  SRR4302547.4003139/1‑94 (MQ=60)
                                                  GTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTA                                      <  SRR4302547.1347585/94‑1 (MQ=60)
                                                   TTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTAT                                     >  SRR4302547.642408/1‑94 (MQ=60)
                                                   TTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTAT                                     <  SRR4302547.3523348/94‑1 (MQ=60)
                                                   TTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTAT                                     <  SRR4302547.3176123/94‑1 (MQ=60)
                                                   TTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTAT                                     <  SRR4302547.378321/94‑1 (MQ=60)
                                                   TTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTAT                                     <  SRR4302547.6320193/94‑1 (MQ=60)
                                                     ATGCCATTTATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCT                                   <  SRR4302547.3371157/94‑1 (MQ=60)
                                                     ATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCT                                   <  SRR4302547.672548/94‑1 (MQ=60)
                                                     ATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCT                                   <  SRR4302547.4106955/94‑1 (MQ=60)
                                                     ATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCT                                   >  SRR4302547.3245432/1‑94 (MQ=60)
                                                      TGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTG                                  <  SRR4302547.4152298/94‑1 (MQ=60)
                                                         CATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCT                               >  SRR4302547.3574216/1‑94 (MQ=60)
                                                         CATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCT                               <  SRR4302547.6022697/94‑1 (MQ=60)
                                                         CATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCT                               >  SRR4302547.594690/1‑94 (MQ=60)
                                                          TTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTA                              >  SRR4302547.2044242/1‑94 (MQ=60)
                                                           TTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAA                             >  SRR4302547.2069245/1‑94 (MQ=60)
                                                           TTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAA                             >  SRR4302547.1345321/1‑94 (MQ=60)
                                                            TGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAAT                            >  SRR4302547.4506246/1‑94 (MQ=60)
                                                             GATGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATG                           <  SRR4302547.163632/94‑1 (MQ=60)
                                                               TGCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAA                         <  SRR4302547.4858451/94‑1 (MQ=60)
                                                                GCTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAA                        <  SRR4302547.338789/94‑1 (MQ=60)
                                                                 CTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAG                       >  SRR4302547.5774204/1‑94 (MQ=60)
                                                                 CTTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAG                       <  SRR4302547.1547925/94‑1 (MQ=60)
                                                                  TTCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGC                      >  SRR4302547.3338500/1‑94 (MQ=60)
                                                                   TCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCT                     <  SRR4302547.5683376/94‑1 (MQ=60)
                                                                   TCGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCT                     <  SRR4302547.3640037/94‑1 (MQ=60)
                                                                    CGAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAATCCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTACTGAAAGCTG                    <  SRR4302547.6628061/94‑1 (MQ=60)
                                                                     GAAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAGAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGG                   >  SRR4302547.2553992/1‑94 (MQ=60)
                                                                      AAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGT                  <  SRR4302547.6641935/94‑1 (MQ=60)
                                                                      AAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGT                  <  SRR4302547.122037/94‑1 (MQ=60)
                                                                      AAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGT                  >  SRR4302547.5132840/1‑94 (MQ=60)
                                                                      AAAATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGG                  >  SRR4302547.4623126/1‑94 (MQ=60)
                                                                       AAATTGCCCGTG‑ACTTGG‑‑GCGGGCTGCCGCAGGAACCCTTTGAAAGGGGAAGGGGAAAAACGGTCCAGTGGTATCTGGCTAATGAAAGCTGGTG                 <  SRR4302547.3823651/94‑1 (MQ=34)
                                                                        AATTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGG                >  SRR4302547.1891589/1‑94 (MQ=60)
                                                                        AAGTGCCCGTGAATTTGGCTGGC‑‑‑TGCAGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGG                <  SRR4302547.1398602/94‑1 (MQ=60)
                                                                          TTGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAA              >  SRR4302547.1718925/1‑94 (MQ=60)
                                                                           TGCCCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAG             >  SRR4302547.4243313/1‑94 (MQ=60)
                                                                              CCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAG          >  SRR4302547.5016811/1‑94 (MQ=60)
                                                                              CCGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAG          >  SRR4302547.2177812/1‑94 (MQ=60)
                                                                               CGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGG         <  SRR4302547.3548467/94‑1 (MQ=60)
                                                                               CGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGG         <  SRR4302547.5615867/94‑1 (MQ=60)
                                                                               CGTGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGG         >  SRR4302547.6250599/1‑94 (MQ=60)
                                                                                 TGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGTATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTG       <  SRR4302547.6748481/94‑1 (MQ=60)
                                                                                 TGAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTG       <  SRR4302547.2583054/94‑1 (MQ=60)
                                                                                  GAACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGC      >  SRR4302547.295486/1‑94 (MQ=60)
                                                                                   AACTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCA     <  SRR4302547.4441869/94‑1 (MQ=60)
                                                                                     CTTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTAGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGG   >  SRR4302547.3287121/1‑94 (MQ=60)
                                                                                      TTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGA  <  SRR4302547.5224194/94‑1 (MQ=60)
                                                                                      TTGGCTGGC‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGA  >  SRR4302547.755967/1‑94 (MQ=60)
                                                                                             |                                                                                         
CTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCG‑‑‑TGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGA  >  NC_000913/3970982‑3971161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑