Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,974,806	C→T	A306V (GCG→GTG)	wzxE →	putative cytochrome

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,974,806	0	C	T	90.6%	704.1 / 44.5	235	A306V (GCG→GTG)	wzxE	putative cytochrome
Reads supporting (aligned to +/- strand): ref base C (13/9); new base T (94/118); total (108/127)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.12e-01

Only 100 of 269 total aligned reads displayed.

TGCTGCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCT  >  NC_000913/3974716‑3974895

tgctgcCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTggcggtgg                                                                                         >  2:2435329/1‑93 (MQ=255)

    gcCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAg                                                                                     >  2:1367904/1‑93 (MQ=255)

     cccACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGCTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGt                                                                                    >  1:6034031/1‑93 (MQ=255)

      ccACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTTGCGTGtt                                                                                   >  1:1235093/1‑93 (MQ=255)

      ccACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAggt                                                                                   >  1:4640546/1‑91 (MQ=255)

      ccACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAggt                                                                                   >  1:2852535/1‑91 (MQ=255)

      ccACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGtt                                                                                   <  1:3559936/93‑1 (MQ=255)

       cACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGttt                                                                                  >  1:4547398/1‑93 (MQ=255)

       cACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGttt                                                                                  <  1:2688872/93‑1 (MQ=255)

        aCGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGtttt                                                                                 >  1:248283/1‑93 (MQ=255)

          gTTGTCGCTGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGG‑TGCGAGTTTTAcc                                                                              >  2:1002463/1‑93 (MQ=255)

          gTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGTAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTAc                                                                               >  2:385591/1‑93 (MQ=255)

           ttGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGGTTTAcc                                                                              >  2:4542760/1‑93 (MQ=255)

            tGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCg                                                                             >  1:5083627/1‑93 (MQ=255)

            tGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGGTTTACCg                                                                             >  1:5616042/1‑93 (MQ=255)

               cgcgGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCTCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCt                                                                          <  2:4170449/93‑1 (MQ=255)

               cgcgGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTTGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCt                                                                          <  1:4953069/93‑1 (MQ=255)

                gcgGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTg                                                                         <  1:3233612/93‑1 (MQ=255)

                gcgGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTg                                                                         <  1:5447020/93‑1 (MQ=255)

                 cgGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTgg                                                                        >  2:5134901/1‑93 (MQ=255)

                 cgGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTgg                                                                        >  2:1488955/1‑93 (MQ=255)

                  ggCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGgc                                                                       <  1:2287186/93‑1 (MQ=255)

                  ggCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGgc                                                                       <  2:6122738/93‑1 (MQ=255)

                  ggCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGgc                                                                       >  1:4450821/1‑93 (MQ=255)

                  ggCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGgc                                                                       <  2:3483198/93‑1 (MQ=255)

                   gCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGgct                                                                      >  2:2700072/1‑93 (MQ=255)

                   gCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGgct                                                                      <  2:164581/93‑1 (MQ=255)

                   gCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGgct                                                                      >  2:1737053/1‑93 (MQ=255)

                   gCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGGTTTACCGTCTGgct                                                                      >  2:3718552/1‑93 (MQ=255)

                    cTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGgctg                                                                     >  2:5294946/1‑93 (MQ=255)

                    cTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGGTTTACCGTCTGgctg                                                                     >  2:5831097/1‑93 (MQ=255)

                     tAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGgctgc                                                                    >  1:4300480/1‑93 (MQ=255)

                     tAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGgctgc                                                                    <  1:88741/93‑1 (MQ=255)

                     tAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGgctgc                                                                    >  1:3674888/1‑93 (MQ=255)

                     tAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGgctgc                                                                    <  2:4835967/93‑1 (MQ=255)

                      aaCGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGgctgct                                                                   >  1:638103/1‑93 (MQ=255)

                       aCGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGctgctg                                                                  >  1:2140573/1‑93 (MQ=255)

                        cGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCtgctgc                                                                 <  2:323369/93‑1 (MQ=255)

                         ggAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCg                                                                >  2:735210/1‑93 (MQ=255)

                          gAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGt                                                               <  2:2476285/93‑1 (MQ=255)

                            aaaGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGa                                                             <  1:6294662/93‑1 (MQ=255)

                             aaGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGAt                                                            <  2:6561191/93‑1 (MQ=255)

                              aGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGAtt                                                           >  1:4199985/1‑93 (MQ=255)

                               gcgcGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGAttt                                                          <  1:945590/93‑1 (MQ=255)

                                 gcgATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTg                                                        >  1:5958279/1‑93 (MQ=255)

                                  cgATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGc                                                       <  1:1415677/93‑1 (MQ=255)

                                  cgATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGc                                                       <  2:4973160/93‑1 (MQ=255)

                                   gATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCt                                                      >  2:4252359/1‑93 (MQ=255)

                                   gATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCCGTGGCGAGGTTTACCGTCTGGCTGCTGCGTGATTTTGCt                                                      >  2:3157881/1‑93 (MQ=255)

                                    gtatCCCCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTa                                                     <  2:2981014/92‑1 (MQ=255)

                                      atCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATc                                                   <  2:3395638/93‑1 (MQ=255)

                                      atCACCCGGGAAGTGCTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATc                                                   <  1:1597314/93‑1 (MQ=255)

                                        cACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTg                                                 <  2:650113/93‑1 (MQ=255)

                                          cccGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTGACCGTCTGGCTGCTGCGTGATTTTGCTATCTGgc                                               <  1:3382364/93‑1 (MQ=255)

                                           ccGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGgct                                              >  2:3538415/1‑93 (MQ=255)

                                               gAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGctgctg                                          >  2:4046561/1‑93 (MQ=255)

                                                aaGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCtgt                                         <  2:4635020/93‑1 (MQ=255)

                                                 aGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGGGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCtgtt                                        <  1:5452439/93‑1 (MQ=255)

                                                  gTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCtgttg                                       >  2:2579717/1‑93 (MQ=255)

                                                  gTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCtgttg                                       <  2:5405649/93‑1 (MQ=255)

                                                     gTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCg                                    <  1:1112431/93‑1 (MQ=255)

                                                     gTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCg                                    <  1:3461903/93‑1 (MQ=255)

                                                      ttAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGa                                   <  1:3281087/93‑1 (MQ=255)

                                                      ttAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGa                                   >  1:414987/1‑93 (MQ=255)

                                                       tAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGaa                                  <  2:2758661/93‑1 (MQ=255)

                                                       tAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGaa                                  >  2:4688711/1‑93 (MQ=255)

                                                        aaaTCTCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGGGATTTTGCTATCTGGCTGCTGTTGTCGAAt                                 <  2:627483/93‑1 (MQ=255)

                                                          aTCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATaa                               <  2:2173898/93‑1 (MQ=255)

                                                           tCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATaaa                              <  2:1948297/93‑1 (MQ=255)

                                                           tCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATaaa                              >  1:215497/1‑93 (MQ=255)

                                                             gCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAAtt                            >  1:164292/1‑93 (MQ=255)

                                                             gCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAAtt                            >  2:3604120/1‑93 (MQ=255)

                                                                gAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTAc                         >  2:798194/1‑93 (MQ=255)

                                                                gAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTAc                         <  2:5552291/93‑1 (MQ=255)

                                                                gAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTAc                         <  1:3266290/93‑1 (MQ=255)

                                                                gAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTAc                         <  2:4891729/93‑1 (MQ=255)

                                                                  aaTTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCg                       >  1:3931756/1‑93 (MQ=255)

                                                                   aTTCGTCTTACCGGCAGTGTCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGc                      <  1:5223278/93‑1 (MQ=255)

                                                                     tCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTa                    <  2:2881857/93‑1 (MQ=255)

                                                                     tCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTa                    <  2:5173182/93‑1 (MQ=255)

                                                                      cGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTAt                   <  2:2037705/93‑1 (MQ=255)

                                                                       gTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATg                  >  2:4231693/1‑93 (MQ=255)

                                                                        tCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATgc                 >  2:569626/1‑93 (MQ=255)

                                                                          ttACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATgcgc               >  2:1829245/1‑93 (MQ=255)

                                                                              cGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGAtc           >  2:1161702/1‑93 (MQ=255)

                                                                               ggCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGAtct          >  1:3692367/1‑93 (MQ=255)

                                                                               ggCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGAtct          <  1:1520248/93‑1 (MQ=255)

                                                                                gCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGAtctc         >  1:5116012/1‑93 (MQ=255)

                                                                                gCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGAtctc         <  2:5754285/93‑1 (MQ=255)

                                                                                 cAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATctct        <  2:6101255/93‑1 (MQ=255)

                                                                                 cAGTGGAGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATctct        <  1:679811/93‑1 (MQ=255)

                                                                                  aGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCtt       <  1:1777409/93‑1 (MQ=255)

                                                                                   gtggcggtggcgAGTTTTATCGTCTGGCTGCTGCGTGGTTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCttt      >  1:6490509/1‑93 (MQ=255)

                                                                                   gtggcggtggcgAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCttt      <  1:2588647/93‑1 (MQ=255)

                                                                                   gtggcggtggcgAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCttt      >  2:3772289/1‑93 (MQ=255)

                                                                                   gTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCttt      >  2:2298345/1‑93 (MQ=255)

                                                                                     ggcggtggcGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGc    <  1:1852205/93‑1 (MQ=255)

                                                                                       cggtggcgAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCt  <  1:750687/93‑1 (MQ=255)

                                                                                       cggtggcgAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCt  <  2:3972807/93‑1 (MQ=255)

                                                                                       cggtggcgAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCt  >  1:1041536/1‑93 (MQ=255)

TGCTGCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCT  >  NC_000913/3974716‑3974895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATTTGCTGCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCTGGCA  >  NC_000913/3974713‑3974899

ATTTGCTGCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGT                                                                                               <  SRR4302547.1419323/94‑1 (MQ=60)

ATTTGCTGCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGT                                                                                               <  SRR4302547.5448397/94‑1 (MQ=60)

ATTTGCTGCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGG                                                                                               >  SRR4302547.1066645/1‑94 (MQ=60)

  TTGCTGCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGG                                                                                             <  SRR4302547.1646995/94‑1 (MQ=60)

   TGCTGCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGC                                                                                            >  SRR4302547.5528832/1‑94 (MQ=60)

   TGCTGCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGC                                                                                            <  SRR4302547.6220259/94‑1 (MQ=60)

    GCTGCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCG                                                                                           <  SRR4302547.2712184/94‑1 (MQ=60)

      TGCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAG                                                                                         >  SRR4302547.652933/1‑94 (MQ=60)

       GCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGCTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGT                                                                                        >  SRR4302547.6159249/1‑94 (MQ=60)

        CCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGGT                                                                                       >  SRR4302547.4736658/1‑94 (MQ=60)

        CCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGGT                                                                                       >  SRR4302547.2909546/1‑94 (MQ=60)

         CCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTT                                                                                      >  SRR4302547.4641445/1‑94 (MQ=60)

         CCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTT                                                                                      <  SRR4302547.3632579/94‑1 (MQ=60)

          CACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTT                                                                                     <  SRR4302547.2742850/94‑1 (MQ=60)

          CACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTT                                                                                     >  SRR4302547.252924/1‑94 (MQ=60)

            CGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTAC                                                                                   >  SRR4302547.2682236/1‑94 (MQ=60)

            CGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTAC                                                                                   >  SRR4302547.6132956/1‑94 (MQ=60)

             GTTGTCGCGGCTACCGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTtcg                                                                                  >  SRR4302547.4208404/1‑91 (MQ=60)

             GTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAACTTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACC                                                                                  >  SRR4302547.2165984/1‑94 (MQ=60)

              TTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCG                                                                                 >  SRR4302547.5725527/1‑94 (MQ=60)

              TTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCG                                                                                 >  SRR4302547.5188500/1‑94 (MQ=60)

              TTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGGTTTACCG                                                                                 >  SRR4302547.5731935/1‑94 (MQ=60)

               TGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGT                                                                                >  SRR4302547.3363293/1‑94 (MQ=60)

                 TCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCT                                                                              <  SRR4302547.5137126/94‑1 (MQ=60)

                 TCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCT                                                                              <  SRR4302547.2234164/94‑1 (MQ=60)

                  CGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTTGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTG                                                                             <  SRR4302547.5055606/94‑1 (MQ=60)

                  CGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTG                                                                             <  SRR4302547.3753342/94‑1 (MQ=60)

                   GCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGG                                                                            <  SRR4302547.5559576/94‑1 (MQ=60)

                   GCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGG                                                                            <  SRR4302547.3298149/94‑1 (MQ=60)

                   GCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGG                                                                            >  SRR4302547.2869546/1‑94 (MQ=60)

                    CGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGC                                                                           >  SRR4302547.4542838/1‑94 (MQ=60)

                     GGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCT                                                                          <  SRR4302547.5259420/94‑1 (MQ=60)

                     GGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCT                                                                          <  SRR4302547.2333494/94‑1 (MQ=60)

                       CTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGC                                                                        >  SRR4302547.3749858/1‑94 (MQ=60)

                       CTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGC                                                                        >  SRR4302547.4388921/1‑94 (MQ=60)

                       CTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGCTTTACCGTCTGGCTGC                                                                        >  SRR4302547.5973608/1‑94 (MQ=60)

                        TAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCT                                                                       >  SRR4302547.649886/1‑94 (MQ=60)

                        TAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCT                                                                       >  SRR4302547.2192590/1‑94 (MQ=60)

                        TAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCT                                                                       <  SRR4302547.90394/94‑1 (MQ=60)

                         AACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTG                                                                      >  SRR4302547.2183256/1‑94 (MQ=60)

                         AACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTG                                                                      >  SRR4302547.220610/1‑94 (MQ=60)

                          ACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGC                                                                     <  SRR4302547.1468850/94‑1 (MQ=60)

                          ACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGC                                                                     <  SRR4302547.6746584/94‑1 (MQ=60)

                            GGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGT                                                                   <  SRR4302547.4457138/94‑1 (MQ=60)

                             GAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTG                                                                  <  SRR4302547.5512831/94‑1 (MQ=60)

                             GAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTG                                                                  >  SRR4302547.3442095/1‑94 (MQ=60)

                              AAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGCTTTACAGTCTGGCTGCTGCGTGA                                                                 >  SRR4302547.1765641/1‑94 (MQ=60)

                               AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGAT                                                                <  SRR4302547.6425511/94‑1 (MQ=60)

                                AAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATT                                                               >  SRR4302547.4285651/1‑94 (MQ=60)

                                AAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGATGTACCGTCTGGCTGCTGCGTGATT                                                               >  SRR4302547.1632364/1‑94 (MQ=60)

                                 AGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTT                                                              >  SRR4302547.977872/1‑94 (MQ=60)

                                 AGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTT                                                              <  SRR4302547.880399/94‑1 (MQ=60)

                                  GCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTT                                                             <  SRR4302547.963926/94‑1 (MQ=60)

                                   CGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTG                                                            <  SRR4302547.3338918/94‑1 (MQ=60)

                                   CGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTG                                                            <  SRR4302547.3736753/94‑1 (MQ=60)

                                   CGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTG                                                            >  SRR4302547.6081825/1‑94 (MQ=60)

                                     CGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCT                                                          <  SRR4302547.1443987/94‑1 (MQ=60)

                                     CGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGGGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCT                                                          >  SRR4302547.869678/1‑94 (MQ=60)

                                     CGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCT                                                          >  SRR4302547.159708/1‑94 (MQ=60)

                                         ATCACCCGGGAAGTGCTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCT                                                      <  SRR4302547.1629012/94‑1 (MQ=60)

                                            ACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGC                                                   <  SRR4302547.4460663/94‑1 (MQ=60)

                                            ACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGC                                                   <  SRR4302547.1845336/94‑1 (MQ=60)

                                             CCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCT                                                  >  SRR4302547.504692/1‑94 (MQ=60)

                                             CCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCT                                                  >  SRR4302547.4660890/1‑94 (MQ=60)

                                             CCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTGACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCT                                                  <  SRR4302547.3451117/94‑1 (MQ=60)

                                               CGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGC                                                >  SRR4302547.955526/1‑94 (MQ=60)

                                                GGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCT                                               <  SRR4302547.4604946/94‑1 (MQ=60)

                                                GGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCT                                               <  SRR4302547.1386307/94‑1 (MQ=60)

                                                 GGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTTGCTGCTGCGTGATTTTGCTATCTGGCTGCTG                                              >  SRR4302547.4849311/1‑94 (MQ=60)

                                                 GGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTG                                              >  SRR4302547.424373/1‑94 (MQ=60)

                                                 GGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGGGGCGAGTTTTACCGTCTGGCTGCTGCGGGATTTTGCTATCTGGCTGCTG                                              >  SRR4302547.3334076/1‑94 (MQ=60)

                                                  GAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGT                                             >  SRR4302547.5246046/1‑94 (MQ=60)

                                                  GAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGT                                             >  SRR4302547.4307063/1‑94 (MQ=60)

                                                   AAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCTGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTT                                            <  SRR4302547.4010143/94‑1 (MQ=60)

                                                    AGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGGGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTG                                           <  SRR4302547.5565110/94‑1 (MQ=60)

                                                    AGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTG                                           <  SRR4302547.2753835/94‑1 (MQ=60)

                                                    AGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTG                                           <  SRR4302547.30023/94‑1 (MQ=60)

                                                      TGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTC                                         <  SRR4302547.973495/94‑1 (MQ=60)

                                                       GGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCG                                        <  SRR4302547.6060987/94‑1 (MQ=60)

                                                        GTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGA                                       <  SRR4302547.1134834/94‑1 (MQ=60)

                                                        GTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGA                                       >  SRR4302547.422682/1‑94 (MQ=60)

                                                        GTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGA                                       <  SRR4302547.3532500/94‑1 (MQ=60)

                                                         TTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAA                                      <  SRR4302547.3346868/94‑1 (MQ=60)

                                                         TTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAA                                      >  SRR4302547.1948456/1‑94 (MQ=60)

                                                           AAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATA                                    <  SRR4302547.3600781/94‑1 (MQ=60)

                                                            AATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAA                                   <  SRR4302547.257269/94‑1 (MQ=60)

                                                            AATCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAA                                   <  SRR4302547.1086450/94‑1 (MQ=60)

                                                             ATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGGATAAA                                  >  SRR4302547.1275449/1‑94 (MQ=60)

                                                             ATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAA                                  >  SRR4302547.219558/1‑94 (MQ=60)

                                                              TCGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAAT                                 <  SRR4302547.6494984/94‑1 (MQ=60)

                                                               CGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGCATAAATT                                >  SRR4302547.4372469/1‑94 (MQ=60)

                                                               CGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATT                                >  SRR4302547.167309/1‑94 (MQ=60)

                                                               CGCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATT                                >  SRR4302547.6504780/1‑94 (MQ=60)

                                                                GCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTT                               <  SRR4302547.5200757/94‑1 (MQ=60)

                                                                GCTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTT                               >  SRR4302547.3981532/1‑94 (MQ=60)

                                                                 CTGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTA                              >  SRR4302547.4614908/1‑94 (MQ=60)

                                                                  TGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTAC                             >  SRR4302547.1205372/1‑94 (MQ=60)

                                                                  TGAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTAC                             >  SRR4302547.1215685/1‑94 (MQ=60)

                                                                  TGAAATTCGTCTTACCGGCAGCGGCGGCGGTGGGCTTTACCGTCTGGCTGCTCGGCGATTTTGGTATCTGGCTGCTGTTcttggattaatttac                             >  SRR4302547.1599457/1‑79 (MQ=37)

                                                                   GAAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACC                            <  SRR4302547.3331639/94‑1 (MQ=60)

                                                                    AAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCG                           <  SRR4302547.2431290/94‑1 (MQ=60)

                                                                    AAATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCG                           >  SRR4302547.4011721/1‑94 (MQ=60)

                                                                     AATTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGC                          >  SRR4302547.4049971/1‑94 (MQ=60)

                                                                      ATTCGTCTTACCGGCAGTGTCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCT                         <  SRR4302547.5330785/94‑1 (MQ=60)

                                                                       TTCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTA                        >  SRR4302547.6425168/1‑94 (MQ=60)

                                                                        TCGTCTTACCGTCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTAT                       <  SRR4302547.4015466/94‑1 (MQ=60)

                                                                        TCGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTAT                       >  SRR4302547.1639501/1‑94 (MQ=60)

                                                                         CGTCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATG                      <  SRR4302547.6684834/94‑1 (MQ=60)

                                                                           TCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCG                    <  SRR4302547.1624230/94‑1 (MQ=60)

                                                                           TCTTACCGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCG                    <  SRR4302547.71511/94‑1 (MQ=60)

                                                                                 CGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCT              >  SRR4302547.3767749/1‑94 (MQ=60)

                                                                                 CGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCT              <  SRR4302547.3648325/94‑1 (MQ=60)

                                                                                 CGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCT              <  SRR4302547.3462302/94‑1 (MQ=60)

                                                                                 CGGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCT              >  SRR4302547.2911462/1‑94 (MQ=60)

                                                                                  GGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTC             <  SRR4302547.1550528/94‑1 (MQ=60)

                                                                                  GGCAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTC             >  SRR4302547.5221432/1‑94 (MQ=60)

                                                                                    CAGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTT           <  SRR4302547.4834037/94‑1 (MQ=60)

                                                                                    CAGTGGAGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTT           <  SRR4302547.692341/94‑1 (MQ=60)

                                                                                     TGTGGCGGTGGCGAGTTTTATCGTCTGGCTGCTGCGTGGTTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTT          >  SRR4302547.6625634/1‑94 (MQ=60)

                                                                                     AGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTT          <  SRR4302547.2467968/94‑1 (MQ=60)

                                                                                     AGTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTT          <  SRR4302547.1812650/94‑1 (MQ=60)

                                                                                     AGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCGGCGGTTGTGGAATAAATTTACCGCTtggggcgttttcttt          >  SRR4302547.3613223/1‑79 (MQ=60)

                                                                                      GTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTG         <  SRR4302547.2640872/94‑1 (MQ=60)

                                                                                      GTGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTG         <  SRR4302547.745044/94‑1 (MQ=60)

                                                                                       TGGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGC        <  SRR4302547.5560331/94‑1 (MQ=60)

                                                                                        GGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCC       <  SRR4302547.1888803/94‑1 (MQ=60)

                                                                                        GGCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCC       <  SRR4302547.1888804/94‑1 (MQ=60)

                                                                                         GCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCT      >  SRR4302547.3306533/1‑94 (MQ=60)

                                                                                         GCGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCT      >  SRR4302547.1062077/1‑94 (MQ=60)

                                                                                          CGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCTG     >  SRR4302547.3473043/1‑94 (MQ=60)

                                                                                          CGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCTG     <  SRR4302547.764840/94‑1 (MQ=60)

                                                                                          CGGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCTG     <  SRR4302547.5688489/94‑1 (MQ=60)

                                                                                           GGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCTGG    >  SRR4302547.1007434/1‑94 (MQ=60)

                                                                                           GGTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCTGG    <  SRR4302547.108328/94‑1 (MQ=60)

                                                                                            GTGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCTGGC   >  SRR4302547.4028014/1‑94 (MQ=60)

                                                                                             TGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCTGGCA  <  SRR4302547.6602011/94‑1 (MQ=60)

ATTTGCTGCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGCTATGCGCGATCTCTTTGCCTGGCA  >  NC_000913/3974713‑3974899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`