Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,001,421 2 bp→GC coding (381‑382/903 nt) rarD ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,001,4210CG100.0% 891.5 / NA 243G128R (GGA→CGA) rarDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (123/120);  total (123/120)
*NC_0009134,001,4220GC99.6% 889.2 / NA 243D127E (GAC→GAG) rarDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (122/120);  minor base A (1/0);  total (123/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

TAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGTTCCAGCATATGGTGATTGTT  >  NC_000913/4001334‑4001508
                                                                                       ||                                                                                      
taataGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAAtcgctcgc                                                                                    >  2:4533991/1‑93 (MQ=255)
 aataGGTAGCGAACCAAAAGTCCACAGCTGGCCTAACCCGCCACATACCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGcc                                                                                   <  1:300253/93‑1 (MQ=255)
 aataGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGcc                                                                                   >  1:6139826/1‑93 (MQ=255)
   taGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGa                                                                                 <  2:6456955/93‑1 (MQ=255)
   taGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGa                                                                                 <  1:4327009/93‑1 (MQ=255)
    aGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAg                                                                                >  1:4890701/1‑93 (MQ=255)
    aGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAg                                                                                >  2:1533857/1‑93 (MQ=255)
      gTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGa                                                                              >  1:1338271/1‑93 (MQ=255)
       tAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGaa                                                                             <  1:4033848/93‑1 (MQ=255)
        aGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGaaa                                                                            <  2:3119615/93‑1 (MQ=255)
         gCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGaaaa                                                                           >  1:2749744/1‑93 (MQ=255)
         gCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGaaaa                                                                           <  1:6576372/93‑1 (MQ=255)
             aCCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAatcat                                                                       >  1:2635145/1‑93 (MQ=255)
             aCCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCAAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAatcat                                                                       <  1:3253785/93‑1 (MQ=255)
              ccAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAatcatc                                                                      >  1:2359088/1‑93 (MQ=255)
              ccAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAatcatc                                                                      <  2:188451/93‑1 (MQ=255)
               cAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATcc                                                                     >  2:5063384/1‑93 (MQ=255)
               cAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATcc                                                                     >  1:1146184/1‑93 (MQ=255)
                aaaaGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATccc                                                                    <  1:2330297/93‑1 (MQ=255)
                aaaaGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATccc                                                                    >  1:2916630/1‑93 (MQ=255)
                aaaaGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATccc                                                                    <  2:5624770/93‑1 (MQ=255)
                 aaaGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATcccc                                                                   >  1:226984/1‑93 (MQ=255)
                  aaGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCa                                                                  <  1:831703/93‑1 (MQ=255)
                  aaGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCa                                                                  <  1:2374895/93‑1 (MQ=255)
                   aGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAg                                                                 <  2:3637228/93‑1 (MQ=255)
                     tCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGca                                                               >  2:1565696/1‑93 (MQ=255)
                      ccACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGcac                                                              <  1:503320/93‑1 (MQ=255)
                       cacaGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGcaca                                                             >  2:199218/1‑93 (MQ=255)
                           gCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGt                                                         >  1:5847516/1‑93 (MQ=255)
                           gCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGt                                                         >  1:3424608/1‑93 (MQ=255)
                              ggACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTcc                                                      >  2:2189664/1‑92 (MQ=255)
                              ggACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCa                                                      >  1:4113482/1‑93 (MQ=255)
                              ggACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCa                                                      >  2:1756922/1‑93 (MQ=255)
                               gACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAc                                                     >  1:4223102/1‑93 (MQ=255)
                               gACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAc                                                     <  1:2579999/93‑1 (MQ=255)
                               gACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAc                                                     <  2:5298071/93‑1 (MQ=255)
                               gACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGAACAATGTTCAc                                                     >  2:553133/1‑93 (MQ=255)
                                 cTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCa                                                   >  2:2322799/1‑93 (MQ=255)
                                 cTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCa                                                   <  2:844319/93‑1 (MQ=255)
                                  tAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAg                                                  >  2:3709580/1‑93 (MQ=255)
                                  tAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAg                                                  >  2:931404/1‑93 (MQ=255)
                                   aaCACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGc                                                 >  1:1483304/1‑93 (MQ=255)
                                     cacGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCgg                                               <  2:2059102/93‑1 (MQ=255)
                                        gCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGtt                                            <  1:358751/93‑1 (MQ=255)
                                        gCCACATATCCCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGtt                                            <  1:6211817/93‑1 (MQ=255)
                                         ccACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTta                                           >  2:4846828/1‑93 (MQ=255)
                                          cccgtatCACCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCCCAATGTTCACCAGCGGGTtaa                                          <  2:3306813/89‑1 (MQ=255)
                                          cacaTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaa                                          >  2:6510713/1‑93 (MQ=255)
                                            caTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGataata                                        >  1:3934020/1‑93 (MQ=255)
                                            caTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaata                                        <  2:5372007/93‑1 (MQ=255)
                                              tatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATaaa                                      <  1:3353781/93‑1 (MQ=255)
                                              tatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATaaa                                      >  1:4793710/1‑93 (MQ=255)
                                              tatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATaaa                                      >  2:5087713/1‑93 (MQ=255)
                                              tatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATaaa                                      <  1:5616165/93‑1 (MQ=255)
                                               ttCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAg                                     <  2:6009186/92‑1 (MQ=255)
                                               atCGCCAGACCCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAg                                     <  1:2402213/93‑1 (MQ=255)
                                               atCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAg                                     <  1:6374786/93‑1 (MQ=255)
                                               atCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAg                                     >  2:3374443/1‑93 (MQ=255)
                                                 cGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTa                                   <  1:6452877/93‑1 (MQ=255)
                                                 cGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTa                                   <  1:1636494/93‑1 (MQ=255)
                                                  gCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTaa                                  >  1:430759/1‑93 (MQ=255)
                                                   ccAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAAc                                 <  2:3835009/93‑1 (MQ=255)
                                                     aGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCa                               >  2:1127567/1‑93 (MQ=255)
                                                     aGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCa                               <  1:2534421/93‑1 (MQ=255)
                                                      gAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCaa                              <  2:5878184/93‑1 (MQ=255)
                                                        aTCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAgg                            >  1:4969180/1‑93 (MQ=255)
                                                         tCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGc                           <  1:3612879/93‑1 (MQ=255)
                                                         gccccGCCAGCCGTTGCATCCGGCCGAATCGCTCGCCGACGAAAATCATCCCCAGCACCATGTTCACCAGCGGGTTAAAAAAGTAACCAAGGc                           <  1:5744524/90‑1 (MQ=255)
                                                          cACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCt                          <  2:3589902/93‑1 (MQ=255)
                                                          cACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCt                          <  1:5331633/93‑1 (MQ=255)
                                                           cccGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTc                         <  1:5915817/92‑1 (MQ=255)
                                                           aCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTc                         >  1:5117838/1‑93 (MQ=255)
                                                           aCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTc                         >  2:68126/1‑93 (MQ=255)
                                                           aCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTc                         >  1:3534359/1‑93 (MQ=255)
                                                            ccGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcg                        <  2:4151167/93‑2 (MQ=255)
                                                            ccGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcg                        <  2:3414215/93‑2 (MQ=255)
                                                             cGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcgc                       <  1:1013139/93‑3 (MQ=255)
                                                              gccagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcgct                      <  2:2997876/93‑4 (MQ=255)
                                                                 agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACGAAGGCTCGCTTcc                   >  1:189062/1‑93 (MQ=255)
                                                                 agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTcc                   >  2:4498137/1‑93 (MQ=255)
                                                                 agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTcc                   >  2:2646266/1‑93 (MQ=255)
                                                                 agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTcc                   <  1:4929158/93‑1 (MQ=255)
                                                                 agccaTCGCATCGGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTcc                   <  2:3991485/93‑1 (MQ=255)
                                                                  gccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCa                  >  1:5487255/1‑93 (MQ=255)
                                                                  gccaTTGCATCCGGCGGAATCGCTCGCCGACGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCa                  >  2:6195507/1‑93 (MQ=255)
                                                                   ccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAg                 >  2:3085821/1‑93 (MQ=255)
                                                                   ccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAg                 <  2:4280719/93‑1 (MQ=255)
                                                                    caTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGc                >  1:4543192/1‑93 (MQ=255)
                                                                     aTTGCGTCCGGCGGAATCGCTAGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCa               <  2:6241391/93‑1 (MQ=255)
                                                                     aTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCa               <  1:5530955/93‑1 (MQ=255)
                                                                        gCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCatat            <  1:1971723/93‑1 (MQ=255)
                                                                          ttcaggcgcAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAAGCTCGCTACCAGCATATgg          <  1:1688065/89‑1 (MQ=255)
                                                                          aTCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATgg          <  2:4909649/93‑1 (MQ=255)
                                                                           tCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGt         >  1:5290552/1‑93 (MQ=255)
                                                                            ccGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTg        >  1:907612/1‑93 (MQ=255)
                                                                              ggcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGAt      >  1:4521923/1‑93 (MQ=255)
                                                                               gcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTGACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGAtt     >  2:4676901/1‑93 (MQ=255)
                                                                               gcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGAtt     >  2:2376170/1‑93 (MQ=255)
                                                                               gcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGAtt     <  2:1353020/93‑1 (MQ=255)
                                                                                  gAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGtt  <  1:5173511/93‑1 (MQ=255)
                                                                                       ||                                                                                      
TAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGTTCCAGCATATGGTGATTGTT  >  NC_000913/4001334‑4001508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGTTCCAGCATATGGTGATTGTTC  >  NC_000913/4001328‑4001509
                                                                                             |                                                                                        
GCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCG                                                                                          <  SRR4302547.6201385/94‑1 (MQ=60)
     ATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGC                                                                                     >  SRR4302547.5676434/1‑94 (MQ=60)
     ATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGC                                                                                     >  SRR4302547.1975111/1‑94 (MQ=60)
      TAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCC                                                                                    >  SRR4302547.6267192/1‑94 (MQ=60)
       AATAGGTAGCGAACCAAAAGTCCACAGCTGGCCTAACCCGCCACATACCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCG                                                                                   <  SRR4302547.305856/94‑1 (MQ=60)
       AATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCG                                                                                   >  SRR4302547.3208897/1‑94 (MQ=60)
         TAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAG                                                                                 >  SRR4302547.4991991/1‑94 (MQ=60)
         TAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAG                                                                                 <  SRR4302547.4416067/94‑1 (MQ=60)
          AGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGG                                                                                <  SRR4302547.4984117/94‑1 (MQ=60)
          AGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGG                                                                                >  SRR4302547.5900294/1‑94 (MQ=60)
           GGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGA                                                                               >  SRR4302547.5361841/1‑94 (MQ=60)
           GGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGA                                                                               >  SRR4302547.2297002/1‑94 (MQ=60)
           GGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGA                                                                               >  SRR4302547.42581/1‑94 (MQ=60)
           GGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGA                                                                               >  SRR4302547.1365170/1‑94 (MQ=60)
            GTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAA                                                                              <  SRR4302547.5067154/94‑1 (MQ=60)
            GTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAA                                                                              >  SRR4302547.3406310/1‑94 (MQ=60)
            GTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAA                                                                              >  SRR4302547.2378353/1‑94 (MQ=60)
             TAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAA                                                                             <  SRR4302547.4115756/94‑1 (MQ=60)
              AGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAA                                                                            >  SRR4302547.2804884/1‑94 (MQ=60)
               GCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAAT                                                                           <  SRR4302547.2415303/94‑1 (MQ=60)
               GCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAAT                                                                           <  SRR4302547.6713289/94‑1 (MQ=60)
                CGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATC                                                                          <  SRR4302547.74591/94‑1 (MQ=60)
                 GAACCAAAAGTCCGCACGTGGACTAACCCGCCAAATATCACAAGAACCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCA                                                                         <  SRR4302547.47220/94‑1 (MQ=32)
                 GAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCA                                                                         <  SRR4302547.6188308/94‑1 (MQ=60)
                  AACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCAT                                                                        <  SRR4302547.2235082/94‑1 (MQ=60)
                  AACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCAT                                                                        >  SRR4302547.2688214/1‑94 (MQ=60)
                  AACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCAT                                                                        <  SRR4302547.3570512/94‑1 (MQ=60)
                   ACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATC                                                                       >  SRR4302547.4380524/1‑94 (MQ=60)
                   ACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATC                                                                       >  SRR4302547.1132669/1‑94 (MQ=60)
                   ACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATC                                                                       >  SRR4302547.2406893/1‑94 (MQ=60)
                   ACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCAAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATC                                                                       <  SRR4302547.3318797/94‑1 (MQ=60)
                    CCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCC                                                                      >  SRR4302547.3608703/1‑94 (MQ=60)
                    CCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCC                                                                      >  SRR4302547.1169358/1‑94 (MQ=60)
                     CAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCC                                                                     >  SRR4302547.2974795/1‑94 (MQ=60)
                      AAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCC                                                                    <  SRR4302547.2377535/94‑1 (MQ=60)
                      AAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCC                                                                    >  SRR4302547.231240/1‑94 (MQ=60)
                       AAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCA                                                                   >  SRR4302547.2405364/1‑94 (MQ=60)
                        AAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAG                                                                  <  SRR4302547.847579/94‑1 (MQ=60)
                        AAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAG                                                                  <  SRR4302547.2423015/94‑1 (MQ=60)
                         AGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGC                                                                 >  SRR4302547.6762774/1‑94 (MQ=60)
                          GTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCA                                                                >  SRR4302547.282117/1‑94 (MQ=60)
                          GTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCA                                                                >  SRR4302547.24325/1‑94 (MQ=60)
                            CCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACA                                                              <  SRR4302547.512483/94‑1 (MQ=60)
                               CAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATG                                                           <  SRR4302547.5932590/94‑1 (MQ=60)
                               CAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATG                                                           >  SRR4302547.2430865/1‑94 (MQ=60)
                                AGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGT                                                          >  SRR4302547.2770230/1‑94 (MQ=60)
                                AGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGT                                                          <  SRR4302547.363926/94‑1 (MQ=60)
                                AGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGT                                                          >  SRR4302547.5968471/1‑94 (MQ=60)
                                AGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGT                                                          >  SRR4302547.3494343/1‑94 (MQ=60)
                                 gataaaaaacaACGCCACATATCCCAAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTT                                                         <  SRR4302547.3928802/83‑1 (MQ=60)
                                 GCTGGACTAACACGCCACATATCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTT                                                         <  SRR4302547.5064558/94‑1 (MQ=60)
                                 GCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGGAAATCATCCCCAGCACAATGTT                                                         >  SRR4302547.4119628/1‑94 (MQ=60)
                                  CTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTC                                                        >  SRR4302547.5386254/1‑94 (MQ=60)
                                  CGGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTC                                                        <  SRR4302547.4947941/94‑1 (MQ=60)
                                   TGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCA                                                       >  SRR4302547.5465983/1‑94 (MQ=60)
                                   TGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCA                                                       >  SRR4302547.4197083/1‑94 (MQ=60)
                                   TGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCA                                                       >  SRR4302547.1717380/1‑94 (MQ=60)
                                    GGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAC                                                      <  SRR4302547.601477/94‑1 (MQ=60)
                                    GGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAC                                                      <  SRR4302547.4412654/94‑1 (MQ=60)
                                    GGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAC                                                      >  SRR4302547.4309357/1‑94 (MQ=60)
                                     GACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACC                                                     <  SRR4302547.2632069/94‑1 (MQ=60)
                                      ACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCA                                                    <  SRR4302547.5168585/94‑1 (MQ=60)
                                       CTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAG                                                   >  SRR4302547.5087322/1‑94 (MQ=60)
                                        TAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGC                                                  >  SRR4302547.1512906/1‑94 (MQ=60)
                                        TAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGC                                                  >  SRR4302547.6273681/1‑94 (MQ=60)
                                         AACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCG                                                 >  SRR4302547.2474498/1‑94 (MQ=60)
                                            ACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGT                                              <  SRR4302547.5413764/94‑1 (MQ=60)
                                             CGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTT                                             <  SRR4302547.6498629/94‑1 (MQ=60)
                                             CGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTA                                             <  SRR4302547.5597093/94‑1 (MQ=60)
                                              GCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTA                                            <  SRR4302547.365408/94‑1 (MQ=60)
                                              GCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTA                                            >  SRR4302547.4468054/1‑94 (MQ=60)
                                              GCCACATATCCCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTA                                            <  SRR4302547.6340813/94‑1 (MQ=60)
                                               CCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAA                                           >  SRR4302547.5629854/1‑94 (MQ=60)
                                                CACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAAT                                          <  SRR4302547.4000600/94‑1 (MQ=60)
                                                 ACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATA                                         <  SRR4302547.1238638/94‑1 (MQ=60)
                                                 ACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGATAATA                                         >  SRR4302547.4014027/1‑94 (MQ=60)
                                                  CATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAA                                        <  SRR4302547.2819028/94‑1 (MQ=60)
                                                   ATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAA                                       >  SRR4302547.6527538/1‑94 (MQ=60)
                                                   ATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAA                                       >  SRR4302547.4893043/1‑94 (MQ=60)
                                                   ATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAA                                       >  SRR4302547.4577551/1‑94 (MQ=60)
                                                    TATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAG                                      <  SRR4302547.1482905/94‑1 (MQ=60)
                                                    TATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAG                                      <  SRR4302547.3421799/94‑1 (MQ=60)
                                                    TATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAG                                      <  SRR4302547.5732060/94‑1 (MQ=60)
                                                     ATCGCCAGACCCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGT                                     <  SRR4302547.2450865/94‑1 (MQ=60)
                                                     ATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGT                                     <  SRR4302547.6507790/94‑1 (MQ=60)
                                                     ATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGT                                     <  SRR4302547.4016258/94‑1 (MQ=60)
                                                       CGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAA                                   >  SRR4302547.2688640/1‑94 (MQ=60)
                                                       CGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAA                                   <  SRR4302547.6587299/94‑1 (MQ=60)
                                                       CGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAA                                   >  SRR4302547.6489970/1‑94 (MQ=60)
                                                       CGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAA                                   <  SRR4302547.1668848/94‑1 (MQ=60)
                                                       CGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAA                                   >  SRR4302547.438738/1‑94 (MQ=60)
                                                        GCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAAC                                  >  SRR4302547.2317234/1‑94 (MQ=60)
                                                        CCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAAC                                  <  SRR4302547.1774348/94‑1 (MQ=60)
                                                         CCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACC                                 <  SRR4302547.5006030/94‑1 (MQ=60)
                                                           AGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAA                               <  SRR4302547.2585638/94‑1 (MQ=60)
                                                             AATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGG                             >  SRR4302547.5072004/1‑94 (MQ=60)
                                                               gccCCGCCAGCCGTTGCATCCGGCCGAATCGCTCGCCGACGAAAATCATCCCCAGCACCATGTTCACCAGCGGGTTAAAAAAGTAACCAAGGCT                           <  SRR4302547.5863228/91‑1 (MQ=60)
                                                               TCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCT                           <  SRR4302547.3686585/94‑1 (MQ=60)
                                                                CACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTC                          >  SRR4302547.2633760/1‑94 (MQ=60)
                                                                CACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTC                          <  SRR4302547.5441520/94‑1 (MQ=60)
                                                                CACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTC                          <  SRR4302547.4374501/94‑1 (MQ=60)
                                                                CACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTC                          <  SRR4302547.3229482/94‑1 (MQ=60)
                                                                CACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTC                          >  SRR4302547.3606524/1‑94 (MQ=60)
                                                                CACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTC                          >  SRR4302547.5223288/1‑94 (MQ=60)
                                                                CACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACAAAGGCTC                          >  SRR4302547.5194846/1‑94 (MQ=60)
                                                                 CCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCG                         <  SRR4302547.6038380/94‑1 (MQ=60)
                                                                  CCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCgc                        >  SRR4302547.2598656/1‑92 (MQ=60)
                                                                   CGCCAGCCATTGCATCCGGCGTAATCGCTCGCCGAGAAAAATCATCCCCAGCTCAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCgct                       <  SRR4302547.961902/94‑4 (MQ=60)
                                                                   CGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCgct                       <  SRR4302547.1032949/94‑4 (MQ=60)
                                                                   CGACAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCgct                       >  SRR4302547.3257225/1‑91 (MQ=60)
                                                                     CCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCgcttc                     >  SRR4302547.2174594/1‑89 (MQ=60)
                                                                      CAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACGAAGGCTCGCTTCC                    >  SRR4302547.192620/1‑94 (MQ=60)
                                                                      CAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCC                    <  SRR4302547.1149641/94‑1 (MQ=60)
                                                                      CAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCC                    <  SRR4302547.3339017/94‑1 (MQ=60)
                                                                       AGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCA                   >  SRR4302547.5600567/1‑94 (MQ=60)
                                                                       AGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCA                   <  SRR4302547.3914585/94‑1 (MQ=60)
                                                                       AGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCA                   <  SRR4302547.5031258/94‑1 (MQ=60)
                                                                       AGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCA                   <  SRR4302547.4735194/94‑1 (MQ=60)
                                                                       AGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCA                   >  SRR4302547.4617638/1‑94 (MQ=60)
                                                                        GCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAG                  <  SRR4302547.4384270/94‑1 (MQ=60)
                                                                        GCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAG                  >  SRR4302547.3472121/1‑94 (MQ=60)
                                                                         CCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGC                 <  SRR4302547.1618719/94‑1 (MQ=60)
                                                                         CCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGC                 >  SRR4302547.4637122/1‑94 (MQ=60)
                                                                          CATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCA                >  SRR4302547.151853/1‑94 (MQ=60)
                                                                          CATTGCATCCGGCGGAATCGATCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGCTAATAAAGTAACCAAGGCTCGCTTCCAGCA                >  SRR4302547.6551738/1‑94 (MQ=60)
                                                                           ATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCAT               >  SRR4302547.344927/1‑94 (MQ=60)
                                                                           ATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCAT               <  SRR4302547.5645115/94‑1 (MQ=60)
                                                                           ATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCAT               <  SRR4302547.6644118/94‑1 (MQ=60)
                                                                            TTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATA              >  SRR4302547.5773132/1‑94 (MQ=60)
                                                                            TTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATA              >  SRR4302547.5727698/1‑94 (MQ=60)
                                                                              GCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATG            <  SRR4302547.5234424/94‑1 (MQ=60)
                                                                              GCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATG            <  SRR4302547.2010878/94‑1 (MQ=60)
                                                                              GCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATG            <  SRR4302547.6123633/94‑1 (MQ=60)
                                                                                ATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGT          >  SRR4302547.1754109/1‑94 (MQ=60)
                                                                                ATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGT          >  SRR4302547.5399688/1‑94 (MQ=60)
                                                                                 TCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTG         >  SRR4302547.925107/1‑94 (MQ=60)
                                                                                 TCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTG         <  SRR4302547.4144066/94‑1 (MQ=60)
                                                                                  CCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGA        >  SRR4302547.3031715/1‑94 (MQ=60)
                                                                                  CCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGA        >  SRR4302547.3667434/1‑94 (MQ=60)
                                                                                  CCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGA        >  SRR4302547.2617229/1‑94 (MQ=60)
                                                                                   CGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGAT       >  SRR4302547.4615366/1‑94 (MQ=60)
                                                                                   CGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGAT       <  SRR4302547.940138/94‑1 (MQ=60)
                                                                                      CGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGT    >  SRR4302547.1219286/1‑94 (MQ=60)
                                                                                      CGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGT    >  SRR4302547.766255/1‑94 (MQ=60)
                                                                                      CGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGT    >  SRR4302547.983950/1‑94 (MQ=60)
                                                                                       GGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTT   <  SRR4302547.4733759/94‑1 (MQ=60)
                                                                                       GGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTT   >  SRR4302547.4896661/1‑94 (MQ=60)
                                                                                       GGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTT   >  SRR4302547.5665343/1‑94 (MQ=60)
                                                                                        GAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTC  <  SRR4302547.3795457/94‑1 (MQ=60)
                                                                                        GAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTC  <  SRR4302547.889968/94‑1 (MQ=60)
                                                                                        GAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTC  <  SRR4302547.5280011/94‑1 (MQ=60)
                                                                                             |                                                                                        
GCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGTTCCAGCATATGGTGATTGTTC  >  NC_000913/4001328‑4001509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑