Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,089,527 G→A P141S (CCG→TCG)  frvB ← PTS system, fructose‑like enzyme IIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,089,5270GA83.3% 636.3 / 95.8 245P141S (CCG→TCG) frvBPTS system, fructose‑like enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base G (24/17);  new base A (102/102);  total (126/119)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.02e-01

Only 100 of 252 total aligned reads displayed.

CGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGG  >  NC_000913/4089435‑4089616
                                                                                            |                                                                                         
cgGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACgg                                                                                           >  1:3523493/1‑93 (MQ=255)
  gcgcgcCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACa                                                                                         <  2:966544/93‑1 (MQ=255)
  gcgcgcCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACa                                                                                         <  2:5827262/93‑1 (MQ=255)
  gcgcgcCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACa                                                                                         >  1:5162424/1‑93 (MQ=255)
  gcgcgcCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACa                                                                                         >  1:4728497/1‑93 (MQ=255)
   cgcgcCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAg                                                                                        <  2:5733381/93‑1 (MQ=255)
   cccgcCTTTCGACATACCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAg                                                                                        <  1:2366061/91‑1 (MQ=255)
    gcgcCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAgc                                                                                       >  1:2226379/1‑93 (MQ=255)
     cgcCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAgcg                                                                                      >  2:4197487/1‑93 (MQ=255)
      gcCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAgcgc                                                                                     <  2:1607358/93‑1 (MQ=255)
      gcCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAgcgc                                                                                     <  2:2795237/93‑1 (MQ=255)
      gcCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAgcgc                                                                                     >  2:1625446/1‑93 (MQ=255)
       ccTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGcgcc                                                                                    >  1:385105/1‑93 (MQ=255)
       ccTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGcgcc                                                                                    >  1:6464779/1‑93 (MQ=255)
         tttCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGcgccgc                                                                                  >  2:5245559/1‑93 (MQ=255)
          ttCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCa                                                                                 >  2:3662907/1‑93 (MQ=255)
          ttCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCa                                                                                 >  2:5998980/1‑93 (MQ=255)
            cGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCaga                                                                               <  2:5048717/93‑1 (MQ=255)
            cGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCaga                                                                               >  2:463107/1‑93 (MQ=255)
             gACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCagag                                                                              <  2:2711082/93‑1 (MQ=255)
             gACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCagag                                                                              <  2:229784/93‑1 (MQ=255)
              aCATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCCCCGCCGATGACAAACGACAGCGCCGCAgaga                                                                             <  1:29318/93‑1 (MQ=255)
              aCATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAgaga                                                                             >  1:4303260/1‑93 (MQ=255)
              aCATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAgaga                                                                             >  1:5428095/1‑93 (MQ=255)
              aCATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAgaga                                                                             <  2:6124514/93‑1 (MQ=255)
              aCATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAgaga                                                                             <  2:2899063/93‑1 (MQ=255)
               cATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGAc                                                                            <  2:1409816/93‑1 (MQ=255)
                atatCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGAcg                                                                           >  1:2431569/1‑93 (MQ=255)
                    cGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGAcgcccg                                                                       <  2:2069641/93‑1 (MQ=255)
                     ggTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGAcgcccgc                                                                      >  2:6511281/1‑93 (MQ=255)
                        ataCGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCAt                                                                   <  1:3768726/93‑1 (MQ=255)
                         taCGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATc                                                                  >  2:273137/1‑93 (MQ=255)
                          actgCAAACGGAACTGCACCAGCATTTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCa                                                                 <  1:2756323/90‑1 (MQ=255)
                          aCGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCa                                                                 <  2:4088456/93‑1 (MQ=255)
                          aCGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCa                                                                 <  2:1400762/93‑1 (MQ=255)
                           cGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAg                                                                <  1:544839/93‑1 (MQ=255)
                           cGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAg                                                                >  2:1221337/1‑93 (MQ=255)
                           cGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAg                                                                >  2:1802767/1‑93 (MQ=255)
                             gCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGt                                                              <  2:6559516/93‑1 (MQ=255)
                             gCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGt                                                              <  2:4529863/93‑1 (MQ=255)
                              cAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTg                                                             >  2:6249018/1‑93 (MQ=255)
                                aaCCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGc                                                           >  2:3216557/1‑93 (MQ=255)
                                  ccGAACGCCCCCACCATTTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCtc                                                         <  1:2309663/93‑1 (MQ=255)
                                     aaCTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCtcatc                                                      >  1:5364384/1‑93 (MQ=255)
                                     aaCTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCtcatc                                                      >  2:4770624/1‑93 (MQ=255)
                                      aCTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCtcatca                                                     >  2:10582/1‑93 (MQ=255)
                                        tGCACCAGCATGTTGGCAAGCGCCACCAGGATACCAGCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACg                                                   >  1:5211399/1‑93 (MQ=255)
                                         gCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGc                                                  <  2:442146/93‑1 (MQ=255)
                                            ccAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTAc                                               <  2:5748236/93‑1 (MQ=255)
                                              aGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCg                                             >  1:1323670/1‑93 (MQ=255)
                                              aGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCg                                             >  1:4678394/1‑93 (MQ=255)
                                               gCATGTTGGCAAGCGCCACCAGGATCCCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGc                                            <  1:2843526/93‑1 (MQ=255)
                                               gCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGc                                            <  2:6180361/93‑1 (MQ=255)
                                               gCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGTCTCATCACGCTACCGc                                            <  2:1706463/93‑1 (MQ=255)
                                               gCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGc                                            >  1:807859/1‑93 (MQ=255)
                                                cATGTTGGCACGCGCCACCAGGATACCACCGCCAATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCt                                           <  2:3819996/93‑1 (MQ=255)
                                                cATGTTGGAAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCt                                           <  1:1824670/93‑1 (MQ=255)
                                                 aTGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCtt                                          <  1:4195413/93‑1 (MQ=255)
                                                    ttGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGc                                       >  2:3726064/1‑93 (MQ=255)
                                                    ttGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGc                                       >  1:3194352/1‑93 (MQ=255)
                                                    tggccAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGc                                       <  2:4918863/89‑1 (MQ=255)
                                                      gacAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCAc                                     <  1:3394925/91‑1 (MQ=255)
                                                         aaGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTc                                  <  1:5579293/93‑1 (MQ=255)
                                                         aaGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTc                                  >  2:5461349/1‑93 (MQ=255)
                                                          aGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTcc                                 <  1:4892706/93‑1 (MQ=255)
                                                          aGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTcc                                 <  2:4802244/93‑1 (MQ=255)
                                                           gcgcCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCt                                <  1:1348787/93‑1 (MQ=255)
                                                            cgcCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTg                               <  1:1885691/93‑1 (MQ=255)
                                                             gcCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGt                              >  2:815728/1‑93 (MQ=255)
                                                                   aGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCa                        >  1:2378111/1‑93 (MQ=255)
                                                                    ggATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCaa                       >  2:1436564/1‑93 (MQ=255)
                                                                    ggATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCaa                       >  2:125901/1‑93 (MQ=255)
                                                                    ggATACCACCGCCGATGACAAAAGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCaa                       >  1:598421/1‑93 (MQ=255)
                                                                     gATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAg                      >  1:1179593/1‑93 (MQ=255)
                                                                      aTACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGc                     >  2:713473/1‑93 (MQ=255)
                                                                      aTACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGc                     >  1:6247929/1‑93 (MQ=255)
                                                                       tACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCt                    >  1:4818254/1‑93 (MQ=255)
                                                                        accaccGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCtt                   <  1:2549868/93‑1 (MQ=255)
                                                                        accaccGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCtt                   >  2:3579617/1‑93 (MQ=255)
                                                                         cccccgccgATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTc                  <  2:2681380/90‑1 (MQ=255)
                                                                         ccaccGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTc                  >  1:960686/1‑93 (MQ=255)
                                                                           cccgccgATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCAc                <  2:6020119/92‑1 (MQ=255)
                                                                             cgccgATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCAcgc              <  2:899804/93‑1 (MQ=255)
                                                                               ccgATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCAcgccg            <  2:6335461/93‑1 (MQ=255)
                                                                               ccgATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCAcgccg            >  1:5665179/1‑93 (MQ=255)
                                                                               ccgATGACAAACGACAGCGCCGAAGAGACGCCCGCCATCAGGTGGCTCATCACGCTCCCGCTTTGCACTTCCTGTTTGCCAAGCTTCAcgccg            <  1:1033375/93‑1 (MQ=255)
                                                                                cgATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCAcgccgc           >  1:4994749/1‑93 (MQ=255)
                                                                                cgATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCAcgccgc           <  1:206700/93‑1 (MQ=255)
                                                                                cgATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCAcgccgc           >  1:4496279/1‑93 (MQ=255)
                                                                                cgATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCAcgccgc           <  2:2994659/93‑1 (MQ=255)
                                                                                 gATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCt          >  2:5821652/1‑93 (MQ=255)
                                                                                 gATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCt          >  2:3428776/1‑93 (MQ=255)
                                                                                 gATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCt          >  2:2207232/1‑93 (MQ=255)
                                                                                  aTGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTTACGCCGCtg         >  2:2634712/1‑92 (MQ=255)
                                                                                   tGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTAt        >  2:6075439/1‑93 (MQ=255)
                                                                                   tGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTAt        >  1:905863/1‑93 (MQ=255)
                                                                                        aaCGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCg   >  2:259003/1‑93 (MQ=255)
                                                                                        aaCGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCg   <  1:1510564/93‑1 (MQ=255)
                                                                                        aaCGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCACTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCg   <  2:3548728/93‑1 (MQ=255)
                                                                                         aCGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCgg  <  1:5858759/93‑1 (MQ=255)
                                                                                            |                                                                                         
CGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGG  >  NC_000913/4089435‑4089616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGGCAAA  >  NC_000913/4089434‑4089620
                                                                                             |                                                                                             
ACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGG                                                                                               >  SRR4302547.3595408/1‑94 (MQ=60)
 CGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGAC                                                                                              >  SRR4302547.4407155/1‑94 (MQ=60)
  GGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACA                                                                                             >  SRR4302547.4826486/1‑94 (MQ=60)
  GGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACA                                                                                             >  SRR4302547.5268715/1‑94 (MQ=60)
    CGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGC                                                                                           >  SRR4302547.4654513/1‑94 (MQ=60)
    CGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGC                                                                                           >  SRR4302547.2271245/1‑94 (MQ=60)
    CGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGC                                                                                           >  SRR4302547.5772942/1‑94 (MQ=60)
    CCCGCCTTTCGACATACCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGC                                                                                           <  SRR4302547.2414021/94‑1 (MQ=60)
      CGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGC                                                                                         >  SRR4302547.2413625/1‑94 (MQ=60)
      CGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGC                                                                                         >  SRR4302547.3451142/1‑94 (MQ=60)
       GCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCC                                                                                        >  SRR4302547.6599396/1‑94 (MQ=60)
       GCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCC                                                                                        >  SRR4302547.392245/1‑94 (MQ=60)
         CTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGC                                                                                      >  SRR4302547.1267629/1‑94 (MQ=60)
         CTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGC                                                                                      >  SRR4302547.2866214/1‑94 (MQ=60)
             CGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACACACGGCAGCGCCGCAGAG                                                                                  >  SRR4302547.4822809/1‑94 (MQ=60)
             CGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAG                                                                                  >  SRR4302547.2914025/1‑94 (MQ=60)
             CGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAG                                                                                  <  SRR4302547.2335295/94‑1 (MQ=60)
              GACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGA                                                                                 >  SRR4302547.4391772/1‑94 (MQ=60)
              GACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGA                                                                                 >  SRR4302547.5540330/1‑94 (MQ=60)
               ACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCCCCGCCGATGACAAACGACAGCGCCGCAGAGAC                                                                                <  SRR4302547.29848/94‑1 (MQ=60)
               ACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCACCGATGACAAACGACAGCGCCGCAGAGAC                                                                                <  SRR4302547.961079/94‑1 (MQ=60)
                CATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACG                                                                               >  SRR4302547.2480758/1‑94 (MQ=60)
                    TCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCG                                                                           >  SRR4302547.2036434/1‑94 (MQ=60)
                    TCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCG                                                                           >  SRR4302547.1885623/1‑94 (MQ=60)
                    TCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCG                                                                           >  SRR4302547.3308737/1‑94 (MQ=60)
                      GGTATAGGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCggagcggccagcc                                                                         >  SRR4302547.2731984/1‑81 (MQ=60)
                      GGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCC                                                                         >  SRR4302547.731510/1‑94 (MQ=60)
                       GTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACACACGACAGCGCCGCAGAGACGCCCGCCC                                                                        >  SRR4302547.5854315/1‑94 (MQ=60)
                        TATACGCCAAACCAAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATCCCCCCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCAT                                                                       <  SRR4302547.3310370/94‑1 (MQ=60)
                         ATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATC                                                                      <  SRR4302547.3845592/94‑1 (MQ=60)
                          TACGGCAAACCGAACTGCACCAGCATGTTGGCGAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCA                                                                     <  SRR4302547.4183755/94‑1 (MQ=60)
                          TACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCA                                                                     >  SRR4302547.2470919/1‑94 (MQ=60)
                          GACGGCAACCCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCCCCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCA                                                                     <  SRR4302547.1600448/94‑1 (MQ=60)
                           ACTGCAAACGGAACTGCACCAGCATTTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAG                                                                    <  SRR4302547.2811574/94‑1 (MQ=60)
                            CGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGG                                                                   <  SRR4302547.554673/94‑1 (MQ=60)
                              GCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTG                                                                 >  SRR4302547.1873171/1‑94 (MQ=60)
                                 AACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCT                                                              <  SRR4302547.3166372/94‑1 (MQ=60)
                                  ACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTC                                                             <  SRR4302547.2259027/94‑1 (MQ=60)
                                   ccgaacgcccccaccattTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCA                                                            <  SRR4302547.2356496/76‑1 (MQ=60)
                                   CCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCA                                                            >  SRR4302547.1459980/1‑94 (MQ=60)
                                     GAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATC                                                          >  SRR4302547.5475041/1‑94 (MQ=60)
                                     GAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATC                                                          >  SRR4302547.4478687/1‑94 (MQ=60)
                                       ACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCCCCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGcttcacaac                                                        >  SRR4302547.2817794/1‑85 (MQ=60)
                                        CTGCACCAGCATGTTGTCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACG                                                       <  SRR4302547.5273118/94‑1 (MQ=60)
                                        CTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCAGCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACG                                                       >  SRR4302547.5318662/1‑94 (MQ=60)
                                        CTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACG                                                       <  SRR4302547.2623480/94‑1 (MQ=60)
                                         TGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCccgt                                                      >  SRR4302547.5590949/1‑90 (MQ=60)
                                           CACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTA                                                    <  SRR4302547.5785588/94‑1 (MQ=60)
                                              CAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCG                                                 >  SRR4302547.1350271/1‑94 (MQ=60)
                                              CAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCG                                                 >  SRR4302547.4775306/1‑94 (MQ=60)
                                               AGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGC                                                >  SRR4302547.823167/1‑94 (MQ=60)
                                               AGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGC                                                >  SRR4302547.226040/1‑94 (MQ=60)
                                                GCATGTTGGCAAGCGCCACCAGGATCCCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCT                                               <  SRR4302547.2900346/94‑1 (MQ=60)
                                                GCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCT                                               >  SRR4302547.6778717/1‑94 (MQ=60)
                                                 CATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTT                                              <  SRR4302547.2410602/94‑1 (MQ=60)
                                                 CATGTTGGAAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTT                                              <  SRR4302547.1860745/94‑1 (MQ=60)
                                                  ATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTT                                             <  SRR4302547.5312004/94‑1 (MQ=60)
                                                  ATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTT                                             <  SRR4302547.4280975/94‑1 (MQ=60)
                                                    GTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGC                                           >  SRR4302547.3258002/1‑94 (MQ=60)
                                                    GTTGCCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGC                                           <  SRR4302547.902972/94‑1 (MQ=60)
                                                      TGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGGGGCTCATCACGCTACCGCTTTGCAC                                         >  SRR4302547.63430/1‑94 (MQ=60)
                                                       GACAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACT                                        <  SRR4302547.3464010/94‑1 (MQ=60)
                                                         CAAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTC                                      >  SRR4302547.6093269/1‑94 (MQ=60)
                                                          AAGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCC                                     <  SRR4302547.5694346/94‑1 (MQ=60)
                                                           AGCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCT                                    <  SRR4302547.4994033/94‑1 (MQ=60)
                                                           AGCGCAACCAGAATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCT                                    >  SRR4302547.3302878/1‑94 (MQ=60)
                                                            GCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTG                                   <  SRR4302547.1375884/94‑1 (MQ=60)
                                                            GCGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTG                                   <  SRR4302547.925900/94‑1 (MQ=60)
                                                             CGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGT                                  >  SRR4302547.4584653/1‑94 (MQ=60)
                                                             CGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGT                                  <  SRR4302547.6277102/94‑1 (MQ=60)
                                                             CGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGT                                  <  SRR4302547.5197545/94‑1 (MQ=60)
                                                             CGCCACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGT                                  <  SRR4302547.1922947/94‑1 (MQ=60)
                                                                CACCAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTG                               <  SRR4302547.5583218/94‑1 (MQ=60)
                                                                  CCGGGATACCCCCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTTTTTGCC                             <  SRR4302547.4292971/94‑1 (MQ=60)
                                                                   CAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCA                            >  SRR4302547.2423763/1‑94 (MQ=60)
                                                                   CAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCA                            >  SRR4302547.5476667/1‑94 (MQ=60)
                                                                   CAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCA                            <  SRR4302547.484262/94‑1 (MQ=60)
                                                                   CAGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCA                            >  SRR4302547.2426294/1‑94 (MQ=60)
                                                                    AGGATCCCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAA                           <  SRR4302547.4935095/94‑1 (MQ=60)
                                                                    AGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAA                           >  SRR4302547.3803285/1‑94 (MQ=60)
                                                                    AGGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGCGGCTCATCACGCTACCGCTCTGCACTTCCTGTTTGCCAA                           >  SRR4302547.1873519/1‑94 (MQ=60)
                                                                    AGGATACCACCGCCGATGACAAAAGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAA                           >  SRR4302547.609457/1‑94 (MQ=60)
                                                                     GGATACCACCGCCGATGATAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAG                          >  SRR4302547.1305103/1‑94 (MQ=60)
                                                                     GGATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAG                          >  SRR4302547.1203420/1‑94 (MQ=60)
                                                                      GATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGC                         >  SRR4302547.6377718/1‑94 (MQ=60)
                                                                       ATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCT                        >  SRR4302547.4918106/1‑94 (MQ=60)
                                                                       ATACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCT                        <  SRR4302547.5544913/94‑1 (MQ=60)
                                                                       ATACCACCGCCGATGAAAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCT                        >  SRR4302547.1423566/1‑94 (MQ=60)
                                                                         ACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTC                      >  SRR4302547.979345/1‑94 (MQ=60)
                                                                         ACCACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTC                      <  SRR4302547.2601359/94‑1 (MQ=60)
                                                                           ctcccCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCAC                    <  SRR4302547.6477152/89‑1 (MQ=60)
                                                                           CACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCAC                    >  SRR4302547.4622013/1‑94 (MQ=60)
                                                                           CACCGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCAC                    <  SRR4302547.6545019/94‑1 (MQ=60)
                                                                              CGCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCC                 <  SRR4302547.2947560/94‑1 (MQ=60)
                                                                               GCCGATGACAAACGACGGCGCCGCAGAGACGCCCGCCATCAGGTGGCGCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCG                <  SRR4302547.2572388/94‑1 (MQ=60)
                                                                               GCCGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCG                >  SRR4302547.5782146/1‑94 (MQ=60)
                                                                                CCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGC               >  SRR4302547.5098025/1‑94 (MQ=60)
                                                                                CCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGC               >  SRR4302547.5083977/1‑94 (MQ=60)
                                                                                CCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGC               >  SRR4302547.4589149/1‑94 (MQ=60)
                                                                                CCGATGACAAACGACAGCGCCGAAGAGACGCCCGCCATCAGGTGGCTCATCACGCTCCCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGC               <  SRR4302547.1053697/94‑1 (MQ=60)
                                                                                 CGATGACAGCCGCCAGCGCCGCAGAGCCGCCCGCCATCAGGTGGCTCATCACGCTCCCGCTTTGCACTTCTTGTTTGCCAAGCTTCCCGCCGCT              <  SRR4302547.5806113/94‑1 (MQ=60)
                                                                                 CGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCT              <  SRR4302547.210583/94‑1 (MQ=60)
                                                                                 CGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCT              >  SRR4302547.4015997/1‑94 (MQ=60)
                                                                                 CGATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCT              >  SRR4302547.5341288/1‑94 (MQ=60)
                                                                                   ATGACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTAT            >  SRR4302547.923318/1‑94 (MQ=60)
                                                                                     GACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCT          <  SRR4302547.5072658/94‑1 (MQ=60)
                                                                                     GACAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCT          <  SRR4302547.1366886/94‑1 (MQ=60)
                                                                                      ACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTG         <  SRR4302547.3883571/94‑1 (MQ=60)
                                                                                       CAAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGC        <  SRR4302547.1112627/94‑1 (MQ=60)
                                                                                        AAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCG       >  SRR4302547.3619225/1‑94 (MQ=60)
                                                                                        AAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCG       <  SRR4302547.3594808/94‑1 (MQ=60)
                                                                                        AAACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCG       <  SRR4302547.4869458/94‑1 (MQ=60)
                                                                                         AACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGG      <  SRR4302547.2473216/94‑1 (MQ=60)
                                                                                         AACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGG      >  SRR4302547.5523186/1‑94 (MQ=60)
                                                                                         AACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGG      <  SRR4302547.1540666/94‑1 (MQ=60)
                                                                                          ACGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGGC     <  SRR4302547.5980002/94‑1 (MQ=60)
                                                                                           CGACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCGGTTTGCCAAGCTTCACGCCGCTAGCTGCGGCA    <  SRR4302547.689905/94‑1 (MQ=60)
                                                                                            GACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGGCAA   <  SRR4302547.4733735/94‑1 (MQ=60)
                                                                                             ACAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGGCAAA  <  SRR4302547.5403930/94‑1 (MQ=60)
                                                                                             |                                                                                             
ACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGGCAAA  >  NC_000913/4089434‑4089620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑