Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,387,877 A→G L21P (CTG→CCG)  psd ← phosphatidylserine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,387,8770AG86.1% 662.6 / 90.6 253L21P (CTG→CCG) psdphosphatidylserine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (14/21);  new base G (105/112);  total (120/133)
Fisher's exact test for biased strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

Only 100 of 265 total aligned reads displayed.

CCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCC  >  NC_000913/4387785‑4387969
                                                                                            |                                                                                            
ccTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCa                                                                                              >  1:6104067/1‑93 (MQ=255)
  tctTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCggg                                                                                            <  2:1951327/93‑1 (MQ=255)
   ctTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGc                                                                                           >  2:4511709/1‑93 (MQ=255)
     ttCATGTCGACCTTGTAGTATTTAACGACCACATCGATAACCAGTGTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGa                                                                                         <  1:1696750/93‑1 (MQ=255)
      tCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAg                                                                                        >  2:2647737/1‑93 (MQ=255)
      tCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAg                                                                                        <  2:1782335/93‑1 (MQ=255)
        aTGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTa                                                                                      <  2:827503/93‑1 (MQ=255)
         tGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTaa                                                                                     <  2:4719922/93‑1 (MQ=255)
             gACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCa                                                                                 >  2:2912541/1‑93 (MQ=255)
             gACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCa                                                                                 >  2:2069057/1‑93 (MQ=255)
              aCCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAATAAGCCAt                                                                                <  1:6501597/93‑1 (MQ=255)
              aCCTTGTAGCATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCAt                                                                                <  2:5632855/93‑1 (MQ=255)
               ccTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATa                                                                               >  1:5772437/1‑93 (MQ=255)
                 ttGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGt                                                                             >  2:4269603/1‑93 (MQ=255)
                    tcgtaTTTAACGAACAGATCGATACCCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCACCCCGCCGGGCGAGTAAGCCATAGTTTc                                                                          <  1:1310942/91‑1 (MQ=255)
                    tagtaTTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTc                                                                          <  2:5209847/93‑1 (MQ=255)
                     agtaTTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCg                                                                         <  2:3719046/93‑1 (MQ=255)
                     agtaTTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCg                                                                         >  2:2669732/1‑93 (MQ=255)
                       taTTTAACGAACAGATCGATAACCAGTTTTATCAGCCATCCAGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGc                                                                       <  1:6653822/93‑1 (MQ=255)
                         tttAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAg                                                                     >  1:6548230/1‑93 (MQ=255)
                         tttAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAg                                                                     >  2:117700/1‑93 (MQ=255)
                         tttAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAg                                                                     <  1:594268/93‑1 (MQ=255)
                          ttAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGa                                                                    <  1:2836680/93‑1 (MQ=255)
                          ttAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGa                                                                    <  1:422726/93‑1 (MQ=255)
                          ttAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGa                                                                    <  1:4189823/93‑1 (MQ=255)
                           tAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGaa                                                                   >  1:3372628/1‑93 (MQ=255)
                                aaCAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTAc                                                              <  2:5052659/93‑1 (MQ=255)
                                aaCAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTAc                                                              >  1:2776420/1‑93 (MQ=255)
                                aaCAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTAc                                                              >  1:3270880/1‑93 (MQ=255)
                                aaCAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTAc                                                              <  1:2986392/93‑1 (MQ=255)
                                aaCAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTAc                                                              >  2:272904/1‑93 (MQ=255)
                                aaCAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTAc                                                              <  2:3893444/93‑1 (MQ=255)
                                 aCAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACt                                                             <  2:2845633/93‑1 (MQ=255)
                                 aCAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACt                                                             >  2:4927162/1‑93 (MQ=255)
                                 aCAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACt                                                             >  2:3496250/1‑93 (MQ=255)
                                   aGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGt                                                           >  2:1247034/1‑93 (MQ=255)
                                   aGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGt                                                           >  1:5720418/1‑93 (MQ=255)
                                   aGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGt                                                           <  1:2712451/93‑1 (MQ=255)
                                   aGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGt                                                           >  1:1421852/1‑93 (MQ=255)
                                    gATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTa                                                          <  2:5790030/93‑1 (MQ=255)
                                      tCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGc                                                        <  1:4329628/93‑1 (MQ=255)
                                       cGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTcgcg                                                       >  1:1332173/1‑93 (MQ=255)
                                       cGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCg                                                       >  2:226397/1‑93 (MQ=255)
                                        gATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGa                                                      <  2:842113/93‑1 (MQ=255)
                                         aTAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGaa                                                     >  1:5101204/1‑93 (MQ=255)
                                         aTAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGaa                                                     <  1:4006485/93‑1 (MQ=255)
                                          tAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGaaa                                                    <  1:221236/93‑1 (MQ=255)
                                           gaCCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAg                                                   >  2:6268461/2‑93 (MQ=255)
                                           aaCCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAg                                                   <  1:1752798/93‑1 (MQ=255)
                                              cAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGttt                                                >  1:4849980/1‑93 (MQ=255)
                                              cAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGttt                                                <  1:6242297/93‑1 (MQ=255)
                                              cAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGttt                                                >  2:5456750/1‑93 (MQ=255)
                                               aGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTa                                               >  2:3446153/1‑93 (MQ=255)
                                                gTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTaa                                              >  1:6150296/1‑93 (MQ=255)
                                                gTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTaa                                              >  2:6389723/1‑93 (MQ=255)
                                                 ttttGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTaaa                                             >  2:4606769/1‑93 (MQ=255)
                                                 ttttGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTaaa                                             <  2:2049716/93‑1 (MQ=255)
                                                  tttGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAAt                                            <  2:4391792/93‑1 (MQ=255)
                                                  tttGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAAt                                            >  2:4472103/1‑93 (MQ=255)
                                                      tCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAAt                                        <  2:2601182/93‑1 (MQ=255)
                                                       cAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCCCCAGGCGGGTAAGCCATAGTTTCGGCGGAATGTACTGTAGCGAAAGTTTAAATGAAtt                                       >  1:943138/1‑93 (MQ=255)
                                                          ccATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTaa                                    <  1:865825/93‑1 (MQ=255)
                                                           cATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAAc                                   <  2:1751/93‑1 (MQ=255)
                                                           cATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAAc                                   >  2:4808458/1‑93 (MQ=255)
                                                           cATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAAc                                   <  1:4031172/93‑1 (MQ=255)
                                                            aTCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACa                                  <  1:2076035/93‑1 (MQ=255)
                                                            aTCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACa                                  >  1:2291187/1‑93 (MQ=255)
                                                            aTCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACa                                  >  1:55025/1‑93 (MQ=255)
                                                            aTCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACa                                  >  1:789427/1‑93 (MQ=255)
                                                             tCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACaa                                 <  2:595366/93‑1 (MQ=255)
                                                             tCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACaa                                 <  1:4604885/93‑1 (MQ=255)
                                                             tCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACaa                                 <  2:38065/93‑1 (MQ=255)
                                                              ccTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAg                                <  1:2568187/93‑1 (MQ=255)
                                                                tGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGt                              <  2:6611059/93‑1 (MQ=255)
                                                                 gCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTa                             <  2:3110739/93‑1 (MQ=255)
                                                                  cccGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAg                            <  1:1035352/93‑1 (MQ=255)
                                                                   ccGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGc                           >  1:4725183/1‑93 (MQ=255)
                                                                    cGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGcc                          <  1:5135505/93‑1 (MQ=255)
                                                                    cGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGcc                          <  1:3552522/93‑1 (MQ=255)
                                                                    cGCTTGCTTGCGCCCCAACCCGCC‑GGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCt                         >  2:3008803/1‑93 (MQ=255)
                                                                      cttgcttgCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTAGGCAGAATGTACTGTAGCGAAAGATTAAATGAATTTAACAAGGTAGCCTc                        >  2:3554755/1‑93 (MQ=255)
                                                                      cttgcttgCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTc                        >  1:5617194/1‑93 (MQ=255)
                                                                       ttgcttgcGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTcc                       <  2:1038095/93‑1 (MQ=255)
                                                                           ttgcGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGGAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGc                   >  1:5405310/1‑93 (MQ=255)
                                                                             gcgcCCCGACCCGCCGGGCGAGTAAGCCATCGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCCCCAGGCCa                 <  1:3457370/93‑1 (MQ=255)
                                                                               gcCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCAtt               >  2:3189767/1‑93 (MQ=255)
                                                                               gcCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCAtt               >  1:3740414/1‑93 (MQ=255)
                                                                               gcCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCAtt               <  1:4973070/93‑1 (MQ=255)
                                                                                ccccAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTg              <  2:365865/93‑1 (MQ=255)
                                                                                    aaCCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGtttt          <  1:1025807/93‑1 (MQ=255)
                                                                                     aCCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTg         >  2:1842166/1‑93 (MQ=255)
                                                                                     aCCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTg         <  1:3018064/93‑1 (MQ=255)
                                                                                     aCCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTg         >  1:3898141/1‑93 (MQ=255)
                                                                                     aCCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTg         <  2:2529800/93‑1 (MQ=255)
                                                                                       ccgccgGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTc       <  1:4087866/93‑1 (MQ=255)
                                                                                        cGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCg      >  1:6308353/1‑93 (MQ=255)
                                                                                        cGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCg      <  2:1399267/93‑1 (MQ=255)
                                                                                         gCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGt     >  2:501757/1‑93 (MQ=255)
                                                                                         gCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGt     >  1:5882111/1‑93 (MQ=255)
                                                                                           cAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGACGTTc   >  2:1628650/1‑93 (MQ=255)
                                                                                            |                                                                                            
CCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCC  >  NC_000913/4387785‑4387969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCT  >  NC_000913/4387784‑4387970
                                                                                             |                                                                                             
GCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCG                                                                                               >  SRR4302547.3158931/1‑94 (MQ=60)
GCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCA                                                                                               >  SRR4302547.6230692/1‑94 (MQ=60)
 CCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGG                                                                                              <  SRR4302547.3774395/94‑1 (MQ=60)
 CCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGG                                                                                              <  SRR4302547.6102286/94‑1 (MQ=60)
  CTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGG                                                                                             >  SRR4302547.5021830/1‑94 (MQ=60)
      TTCATGTCGACCTTGTAGTATTTAACGACCACATCGATAACCAGTGTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAG                                                                                         <  SRR4302547.1730112/94‑1 (MQ=60)
      TTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAG                                                                                         <  SRR4302547.4376750/94‑1 (MQ=60)
       TCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGT                                                                                        <  SRR4302547.6020624/94‑1 (MQ=60)
         ATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAA                                                                                      >  SRR4302547.27863/1‑94 (MQ=60)
          TGTCGACCTTGTAGTATTTAACGAACAGATCGATAACGAGTTTTGTCAGCCAGCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAG                                                                                     <  SRR4302547.1574725/94‑1 (MQ=60)
           GTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGC                                                                                    >  SRR4302547.6532747/1‑94 (MQ=60)
            TCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCC                                                                                   <  SRR4302547.3424897/94‑1 (MQ=60)
              GACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCAT                                                                                 <  SRR4302547.4670067/94‑1 (MQ=60)
              GACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCAT                                                                                 <  SRR4302547.3204433/94‑1 (MQ=60)
               ACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATA                                                                                >  SRR4302547.5891824/1‑94 (MQ=60)
               ACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATA                                                                                >  SRR4302547.4404410/1‑94 (MQ=60)
               ACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATA                                                                                <  SRR4302547.72485/94‑1 (MQ=60)
               ACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATA                                                                                <  SRR4302547.1222017/94‑1 (MQ=60)
               ACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAATAAGCCATA                                                                                <  SRR4302547.6636932/94‑1 (MQ=60)
                 CTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGT                                                                              >  SRR4302547.6242254/1‑94 (MQ=60)
                 CTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGT                                                                              >  SRR4302547.2006781/1‑94 (MQ=60)
                 CTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGT                                                                              >  SRR4302547.2365842/1‑94 (MQ=60)
                   TGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTT                                                                            >  SRR4302547.403791/1‑94 (MQ=60)
                   TGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTT                                                                            >  SRR4302547.730451/1‑94 (MQ=60)
                   TGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTT                                                                            <  SRR4302547.6467071/94‑1 (MQ=60)
                     TCGTATTTAACGAACAGATCGATACCCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCACCCCGCCGGGCGAGTAAGCCATAGTTTCA                                                                          <  SRR4302547.1337303/94‑1 (MQ=60)
                      AGTATTTAACGAGCAGATCGATAACCAGTTTTGTCAGCCACCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGG                                                                         <  SRR4302547.1700205/94‑1 (MQ=60)
                        TATTTAACGAACAGATCGATAACCAGTTTTATCAGCCATCCAGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCA                                                                       <  SRR4302547.6792620/94‑1 (MQ=60)
                         ATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAG                                                                      >  SRR4302547.897189/1‑94 (MQ=60)
                         ATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAG                                                                      >  SRR4302547.6684504/1‑94 (MQ=60)
                         ATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAG                                                                      <  SRR4302547.5954691/94‑1 (MQ=60)
                          TTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGA                                                                     <  SRR4302547.605141/94‑1 (MQ=60)
                           TTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAA                                                                    >  SRR4302547.3441132/1‑94 (MQ=60)
                           TTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAA                                                                    <  SRR4302547.2893388/94‑1 (MQ=60)
                           TTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAA                                                                    <  SRR4302547.430557/94‑1 (MQ=60)
                           TTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAA                                                                    <  SRR4302547.4275265/94‑1 (MQ=60)
                               CGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTA                                                                >  SRR4302547.4976501/1‑94 (MQ=60)
                                GAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTAC                                                               >  SRR4302547.2832037/1‑94 (MQ=60)
                                GAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTAC                                                               <  SRR4302547.6064906/94‑1 (MQ=60)
                                GAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTAC                                                               >  SRR4302547.3336364/1‑94 (MQ=60)
                                GAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTAC                                                               <  SRR4302547.4147724/94‑1 (MQ=60)
                                 AACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAGGCCATAGTTTCGGCAGAATGTACT                                                              >  SRR4302547.5236845/1‑94 (MQ=60)
                                 AACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACT                                                              <  SRR4302547.3045810/94‑1 (MQ=60)
                                  ACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTG                                                             >  SRR4302547.5682421/1‑94 (MQ=60)
                                  ACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTG                                                             >  SRR4302547.6385008/1‑94 (MQ=60)
                                  ACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTG                                                             >  SRR4302547.5824252/1‑94 (MQ=60)
                                   CAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGT                                                            >  SRR4302547.5838592/1‑94 (MQ=60)
                                   CAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGT                                                            >  SRR4302547.1450323/1‑94 (MQ=60)
                                   CAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGT                                                            <  SRR4302547.4222819/94‑1 (MQ=60)
                                    AGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTA                                                           >  SRR4302547.3414940/1‑94 (MQ=60)
                                    AGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTA                                                           <  SRR4302547.2766904/94‑1 (MQ=60)
                                    AGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTA                                                           <  SRR4302547.3655130/94‑1 (MQ=60)
                                    AGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTA                                                           >  SRR4302547.2663492/1‑94 (MQ=60)
                                     GATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAG                                                          <  SRR4302547.3541711/94‑1 (MQ=60)
                                       TCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTCGCG                                                        >  SRR4302547.1358948/1‑94 (MQ=60)
                                       TCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCG                                                        <  SRR4302547.4418718/94‑1 (MQ=60)
                                       TCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCG                                                        >  SRR4302547.3053183/1‑94 (MQ=60)
                                         GATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAA                                                      >  SRR4302547.4891580/1‑94 (MQ=60)
                                         GATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAA                                                      >  SRR4302547.5206369/1‑94 (MQ=60)
                                         GATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAA                                                      <  SRR4302547.1780158/94‑1 (MQ=60)
                                          ATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAA                                                     <  SRR4302547.4087916/94‑1 (MQ=60)
                                           TAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAG                                                    >  SRR4302547.1666082/1‑94 (MQ=60)
                                           TAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAG                                                    <  SRR4302547.225400/94‑1 (MQ=60)
                                            AACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGT                                                   <  SRR4302547.1787449/94‑1 (MQ=60)
                                            AACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGT                                                   >  SRR4302547.4310477/1‑94 (MQ=60)
                                             ACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTT                                                  <  SRR4302547.3681735/94‑1 (MQ=60)
                                              CCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTT                                                 <  SRR4302547.1388495/94‑1 (MQ=60)
                                              CCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTT                                                 <  SRR4302547.2386478/94‑1 (MQ=60)
                                              CCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTT                                                 >  SRR4302547.4950485/1‑94 (MQ=60)
                                               CAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTA                                                <  SRR4302547.6371978/94‑1 (MQ=60)
                                                AGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAA                                               >  SRR4302547.6277900/1‑94 (MQ=60)
                                                AGTTTTGTCAGCCAGCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAA                                               <  SRR4302547.6371481/94‑1 (MQ=60)
                                                 GTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAA                                              >  SRR4302547.959626/1‑94 (MQ=60)
                                                  TTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAAT                                             <  SRR4302547.3506626/94‑1 (MQ=60)
                                                    TTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGA                                           >  SRR4302547.4252271/1‑94 (MQ=60)
                                                    TTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGA                                           <  SRR4302547.1312690/94‑1 (MQ=60)
                                                      GTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAAT                                         <  SRR4302547.3105943/94‑1 (MQ=60)
                                                       TCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCCCCAGGCGGGTAAGCCATAGTTTCGGCGGAATGTACTGTAGCGAAAGTTTAAATGAATT                                        >  SRR4302547.961417/1‑94 (MQ=60)
                                                        CAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTT                                       <  SRR4302547.5877503/94‑1 (MQ=60)
                                                         AGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTA                                      <  SRR4302547.4789391/94‑1 (MQ=60)
                                                         AGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTA                                      >  SRR4302547.2282662/1‑94 (MQ=60)
                                                         AGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTA                                      >  SRR4302547.2016135/1‑94 (MQ=60)
                                                           CCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAAC                                    <  SRR4302547.882391/94‑1 (MQ=60)
                                                           CCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAAC                                    <  SRR4302547.6397830/94‑1 (MQ=60)
                                                            CATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACA                                   >  SRR4302547.56057/1‑94 (MQ=60)
                                                            CATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACA                                   >  SRR4302547.2337601/1‑94 (MQ=60)
                                                            CATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACA                                   >  SRR4302547.804372/1‑94 (MQ=60)
                                                            CATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACA                                   <  SRR4302547.4113050/94‑1 (MQ=60)
                                                             ATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAA                                  <  SRR4302547.2117446/94‑1 (MQ=60)
                                                              TCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAG                                 <  SRR4302547.844140/94‑1 (MQ=60)
                                                              TCCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAG                                 <  SRR4302547.4700177/94‑1 (MQ=60)
                                                               CCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGG                                <  SRR4302547.2124766/94‑1 (MQ=60)
                                                               CCTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGG                                <  SRR4302547.2620053/94‑1 (MQ=60)
                                                                CTGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGT                               <  SRR4302547.2761854/94‑1 (MQ=60)
                                                                 TGCCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTA                              >  SRR4302547.5229128/1‑94 (MQ=60)
                                                                   CCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGC                            <  SRR4302547.1055438/94‑1 (MQ=60)
                                                                   CCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGC                            >  SRR4302547.6753201/1‑94 (MQ=60)
                                                                   CCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGC                            <  SRR4302547.1055731/94‑1 (MQ=60)
                                                                   CCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGC                            >  SRR4302547.3530681/1‑94 (MQ=60)
                                                                   CCCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGC                            >  SRR4302547.4823078/1‑94 (MQ=60)
                                                                    CCGCTTGCTTGCGCCCCAGCCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCC                           <  SRR4302547.6730575/94‑1 (MQ=60)
                                                                    CCGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCC                           <  SRR4302547.400982/94‑1 (MQ=60)
                                                                     CGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCT                          >  SRR4302547.5790297/1‑94 (MQ=60)
                                                                     CGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCT                          <  SRR4302547.3625026/94‑1 (MQ=60)
                                                                     CGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCT                          <  SRR4302547.5241310/94‑1 (MQ=60)
                                                                     CGCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCT                          >  SRR4302547.2884379/1‑94 (MQ=60)
                                                                      GCTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTC                         <  SRR4302547.4663081/94‑1 (MQ=60)
                                                                      GCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTC                         >  SRR4302547.5733109/1‑94 (MQ=60)
                                                                      GCTTGCTTACGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGATTAAATGAATTTAACAAGGTAGCCTC                         >  SRR4302547.6673109/1‑94 (MQ=60)
                                                                       CTTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCC                        >  SRR4302547.535957/1‑94 (MQ=60)
                                                                       CTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCC                        <  SRR4302547.5090147/94‑1 (MQ=60)
                                                                        TTGCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGCAGCCTCCA                       >  SRR4302547.409212/1‑94 (MQ=60)
                                                                          GCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGG                     <  SRR4302547.3817603/94‑1 (MQ=60)
                                                                          GCTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAAAGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGG                     >  SRR4302547.5449956/1‑94 (MQ=60)
                                                                           CTTGCGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGGAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGC                    >  SRR4302547.5517052/1‑94 (MQ=60)
                                                                           CTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGC                    >  SRR4302547.5553291/1‑94 (MQ=60)
                                                                              GCGCCCCGACCCGCCGGGCGAGTAAGCCATCGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCCCCAGGCCAT                 <  SRR4302547.3527859/94‑1 (MQ=60)
                                                                               CGCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATT                >  SRR4302547.3816779/1‑94 (MQ=60)
                                                                                GCCCCAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTG               >  SRR4302547.6276669/1‑94 (MQ=60)
                                                                                GCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTG               <  SRR4302547.5075952/94‑1 (MQ=60)
                                                                                    CAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTT           <  SRR4302547.1445396/94‑1 (MQ=60)
                                                                                    CAACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTT           >  SRR4302547.5702874/1‑94 (MQ=60)
                                                                                     AACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTG          <  SRR4302547.1045946/94‑1 (MQ=60)
                                                                                     AACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTG          <  SRR4302547.41604/94‑1 (MQ=60)
                                                                                     AACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTG          <  SRR4302547.2906603/94‑1 (MQ=60)
                                                                                     AACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTG          >  SRR4302547.3977399/1‑94 (MQ=60)
                                                                                     AACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTG          <  SRR4302547.2888680/94‑1 (MQ=60)
                                                                                      ACCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGT         <  SRR4302547.3078077/94‑1 (MQ=60)
                                                                                      ACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGT         >  SRR4302547.6003265/1‑94 (MQ=60)
                                                                                       CCCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTC        >  SRR4302547.4904951/1‑94 (MQ=60)
                                                                                        CCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCG       >  SRR4302547.6439501/1‑94 (MQ=60)
                                                                                        CCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCG       <  SRR4302547.2906751/94‑1 (MQ=60)
                                                                                        CCGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCG       <  SRR4302547.4170972/94‑1 (MQ=60)
                                                                                         CGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGT      >  SRR4302547.6003899/1‑94 (MQ=60)
                                                                                         CGCCGGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGT      >  SRR4302547.1339068/1‑94 (MQ=60)
                                                                                          GCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTT     <  SRR4302547.6075513/94‑1 (MQ=60)
                                                                                           CCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTC    >  SRR4302547.2202564/1‑94 (MQ=60)
                                                                                             GGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCT  <  SRR4302547.6205145/94‑1 (MQ=60)
                                                                                             GGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCT  <  SRR4302547.6048316/94‑1 (MQ=60)
                                                                                             GGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCT  >  SRR4302547.5262994/1‑94 (MQ=60)
                                                                                             AGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCT  >  SRR4302547.3773716/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCT  >  NC_000913/4387784‑4387970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑