Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,463,718 A→G L13L (TTG→CTG)  treB ← PTS trehalose‑specific system enzyme IIBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,463,7180AG82.4% 517.6 / 98.0 218L13L (TTG→CTG) treBPTS trehalose‑specific system enzyme IIBC
Reads supporting (aligned to +/- strand):  ref base A (17/21);  new base G (86/92);  total (105/113)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 236 total aligned reads displayed.

CATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCGGAAA  >  NC_000913/4463628‑4463809
                                                                                          |                                                                                           
 aTTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCcg                                                                                          <  1:6442072/93‑1 (MQ=255)
 aTTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCcg                                                                                          <  1:4417355/93‑1 (MQ=255)
 aTTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCcg                                                                                          >  2:4626470/1‑93 (MQ=255)
  ttGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCcga                                                                                         <  1:1120781/93‑1 (MQ=255)
  ttGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCcga                                                                                         <  1:309078/93‑1 (MQ=255)
   tGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCcgat                                                                                        <  2:3827544/93‑1 (MQ=255)
    ggCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACcgatc                                                                                       >  1:2310214/1‑93 (MQ=255)
     gCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCcgatcg                                                                                      >  2:4728070/1‑93 (MQ=255)
      ccGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCcgatcga                                                                                     >  2:57569/1‑93 (MQ=255)
      ccGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCcgatcga                                                                                     <  1:3532510/93‑1 (MQ=255)
       cGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCcgatcgat                                                                                    >  1:1652415/1‑93 (MQ=255)
         gttggttgAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGatat                                                                                  <  2:2205474/93‑1 (MQ=255)
         gttggttgAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGatat                                                                                  >  2:339707/1‑93 (MQ=255)
         gttggttgAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGatat                                                                                  >  2:3532063/1‑93 (MQ=255)
         gttggttgAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGatat                                                                                  <  1:5008659/93‑1 (MQ=255)
          ttggttgAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATc                                                                                 <  1:4762191/93‑1 (MQ=255)
          ttggttgAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATc                                                                                 <  2:414154/93‑1 (MQ=255)
          ttggttgAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACAGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATc                                                                                 <  2:6240919/93‑1 (MQ=255)
           tggttgAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATcc                                                                                >  1:2785721/1‑93 (MQ=255)
           tggttgAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATcc                                                                                <  2:6167213/93‑1 (MQ=255)
           tggtggAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATcc                                                                                <  2:4859413/93‑1 (MQ=255)
            ggttgAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCg                                                                               >  1:2006260/1‑93 (MQ=255)
            ggttgAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCg                                                                               >  1:3762136/1‑93 (MQ=255)
             gttgAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGt                                                                              >  1:593474/1‑93 (MQ=255)
               tgAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGttt                                                                            <  2:1687154/93‑1 (MQ=255)
                  ggACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGt                                                                         <  1:369189/93‑1 (MQ=255)
                   gACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGtt                                                                        <  2:4277112/93‑1 (MQ=255)
                    aCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGttt                                                                       >  1:733100/1‑93 (MQ=255)
                     cAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTa                                                                      <  1:5114567/93‑1 (MQ=255)
                       aaGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCCACCGATCGATATCCGTTTGGTTTAtt                                                                    >  1:3339647/1‑93 (MQ=255)
                       aaGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTAtt                                                                    <  1:2883447/93‑1 (MQ=255)
                       aaGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTAtt                                                                    <  1:6567940/93‑1 (MQ=255)
                       aaGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTAtt                                                                    >  2:1468676/1‑93 (MQ=255)
                        aGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTAttt                                                                   >  2:4051350/1‑93 (MQ=255)
                         gCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTAtttt                                                                  >  2:2754831/1‑93 (MQ=255)
                         gCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTAtttt                                                                  <  1:1968455/93‑1 (MQ=255)
                          cGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTg                                                                 <  2:1141902/93‑1 (MQ=255)
                            tAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCt                                                               >  2:2463195/1‑93 (MQ=255)
                            tAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCt                                                               >  1:3268981/1‑93 (MQ=255)
                              ggCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCtca                                                             <  2:4387221/93‑1 (MQ=255)
                              ggCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCtca                                                             <  2:3760189/93‑1 (MQ=255)
                                cGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCtcatc                                                           >  1:54150/1‑93 (MQ=255)
                                cGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCtcatc                                                           <  1:2554250/93‑1 (MQ=255)
                                 gAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCtcatca                                                          <  1:1881264/93‑1 (MQ=255)
                                 gAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCAGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCtcatca                                                          >  2:3559123/1‑93 (MQ=255)
                                    tAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATaa                                                       >  2:6054853/1‑93 (MQ=255)
                                    tAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATaa                                                       <  1:6540313/93‑1 (MQ=255)
                                     aaTACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATaaa                                                      <  2:5617160/93‑1 (MQ=255)
                                     aaTACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATaaa                                                      <  1:1294273/93‑1 (MQ=255)
                                       tACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGc                                                    >  2:5327654/1‑93 (MQ=255)
                                         cAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGccc                                                  >  1:5615095/1‑93 (MQ=255)
                                          aGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGcccc                                                 >  2:1211995/1‑93 (MQ=255)
                                           gTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCa                                                <  1:2359082/93‑1 (MQ=255)
                                            tGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCAt                                               <  2:873488/93‑1 (MQ=255)
                                            tGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCAt                                               <  2:5139083/93‑1 (MQ=255)
                                               ctaaCCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGc                                            <  1:5840079/90‑1 (MQ=255)
                                               cTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGc                                            >  1:2705352/1‑93 (MQ=255)
                                               cTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGc                                            <  1:61503/93‑1 (MQ=255)
                                               cTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGc                                            <  1:6003146/93‑1 (MQ=255)
                                                tCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCa                                           >  2:5399175/1‑93 (MQ=255)
                                                  cccGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGa                                         <  1:2732437/92‑1 (MQ=255)
                                                  aCCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGa                                         <  1:3392125/93‑1 (MQ=255)
                                                   ccGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGAt                                        >  2:4542127/1‑93 (MQ=255)
                                                    cGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATg                                       <  2:5880497/93‑1 (MQ=255)
                                                    cGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATg                                       <  1:218169/93‑1 (MQ=255)
                                                      tCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGAc                                     <  2:3214575/93‑1 (MQ=255)
                                                       cGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACa                                    >  1:4093446/1‑93 (MQ=255)
                                                       cGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACa                                    >  2:136138/1‑93 (MQ=255)
                                                          aaTATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACAttt                                 >  2:2854255/1‑93 (MQ=255)
                                                              ttGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTttgg                             >  1:1508590/1‑93 (MQ=255)
                                                              ttGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTttgg                             <  1:5439968/93‑1 (MQ=255)
                                                              ttGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTttgg                             >  1:2494483/1‑93 (MQ=255)
                                                               tGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTttggt                            <  1:2961009/93‑1 (MQ=255)
                                                                   gcgcCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGc                        <  1:6624279/93‑1 (MQ=255)
                                                                      cccgccGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGc                     <  2:2358116/93‑1 (MQ=255)
                                                                      cccgccGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGc                     >  1:1387078/1‑93 (MQ=255)
                                                                       ccgccgACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCa                    >  1:660984/1‑93 (MQ=255)
                                                                          ccgACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGaa                 <  1:3832374/93‑1 (MQ=255)
                                                                           cgACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAat                >  2:3721358/1‑93 (MQ=255)
                                                                           cgACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAat                >  2:2615336/1‑93 (MQ=255)
                                                                           cgACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAat                >  2:1724854/1‑93 (MQ=255)
                                                                            gACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAata               <  2:2001278/93‑1 (MQ=255)
                                                                            gACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAata               <  1:4030360/93‑1 (MQ=255)
                                                                             aCCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAatat              <  1:6468092/93‑1 (MQ=255)
                                                                             aCCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAatat              >  1:4263980/1‑93 (MQ=255)
                                                                               cAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAatatat            >  2:5088219/1‑93 (MQ=255)
                                                                                aGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATAtt           <  2:4450326/93‑1 (MQ=255)
                                                                                aGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATAtt           <  1:1037791/93‑1 (MQ=255)
                                                                                aGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATAtt           >  1:4976256/1‑93 (MQ=255)
                                                                                 gTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTg          <  1:6199825/93‑1 (MQ=255)
                                                                                  ttCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTgc         >  2:3969429/1‑93 (MQ=255)
                                                                                  ttCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTgc         <  2:2500531/93‑1 (MQ=255)
                                                                                  ttCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTgc         <  2:6288538/93‑1 (MQ=255)
                                                                                   tcaatcagCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTgcg        <  1:4516953/93‑1 (MQ=255)
                                                                                   tcaatcaaCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTgcg        >  1:4676525/1‑93 (MQ=255)
                                                                                     aatcagCCGATCGATATCCGTTTGGTGTAGTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGcgcg      <  2:6617707/93‑1 (MQ=255)
                                                                                      atcagCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCgg     >  2:4934309/1‑93 (MQ=255)
                                                                                      atcagCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCgg     <  2:2113603/93‑1 (MQ=255)
                                                                                       tcagCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCGGa    >  2:4045238/1‑93 (MQ=255)
                                                                                         aaCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCGGaaa  >  1:5733326/1‑93 (MQ=255)
                                                                                          |                                                                                           
CATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCGGAAA  >  NC_000913/4463628‑4463809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCGGAAAA  >  NC_000913/4463626‑4463810
                                                                                            |                                                                                            
GGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGC                                                                                             <  SRR4302547.956806/94‑1 (MQ=60)
GGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGC                                                                                             >  SRR4302547.5617026/1‑94 (MQ=60)
  CATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCG                                                                                           >  SRR4302547.2749840/1‑94 (MQ=60)
  CATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCG                                                                                           <  SRR4302547.6269899/94‑1 (MQ=60)
   ATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGA                                                                                          <  SRR4302547.6576324/94‑1 (MQ=60)
   ATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGA                                                                                          <  SRR4302547.4508517/94‑1 (MQ=60)
   ATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGA                                                                                          >  SRR4302547.3856782/1‑94 (MQ=60)
    TTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGAT                                                                                         <  SRR4302547.1143382/94‑1 (MQ=60)
    TTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGAT                                                                                         <  SRR4302547.314826/94‑1 (MQ=60)
     TGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATC                                                                                        >  SRR4302547.2357055/1‑94 (MQ=60)
        CCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGAT                                                                                     <  SRR4302547.3604638/94‑1 (MQ=60)
        CCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGAT                                                                                     >  SRR4302547.1685018/1‑94 (MQ=60)
         CGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATA                                                                                    <  SRR4302547.5900511/94‑1 (MQ=60)
          GGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATAT                                                                                   <  SRR4302547.6315227/94‑1 (MQ=60)
          GGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATAT                                                                                   >  SRR4302547.915673/1‑94 (MQ=60)
          GGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATAT                                                                                   <  SRR4302547.5208540/94‑1 (MQ=60)
           GTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATC                                                                                  <  SRR4302547.5112216/94‑1 (MQ=60)
            TTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCC                                                                                 >  SRR4302547.2841513/1‑94 (MQ=60)
            TTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCC                                                                                 <  SRR4302547.4860853/94‑1 (MQ=60)
             TGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCG                                                                                >  SRR4302547.3838884/1‑94 (MQ=60)
             TGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCG                                                                                >  SRR4302547.2046181/1‑94 (MQ=60)
              GGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGT                                                                               >  SRR4302547.604322/1‑94 (MQ=60)
               GTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTT                                                                              >  SRR4302547.5413469/1‑94 (MQ=60)
                 TGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGTCGATCGATATCCGTTTG                                                                            >  SRR4302547.255174/1‑94 (MQ=60)
                  GAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGG                                                                           >  SRR4302547.2047673/1‑94 (MQ=60)
                   AGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGT                                                                          <  SRR4302547.5506476/94‑1 (MQ=60)
                    GGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTT                                                                         <  SRR4302547.376033/94‑1 (MQ=60)
                     GACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTT                                                                        <  SRR4302547.3446728/94‑1 (MQ=60)
                     GACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTT                                                                        >  SRR4302547.746793/1‑94 (MQ=60)
                       CAAAGCGTGGCCTAGTAATACAGTAGCTCACCGTCGCAATATTGCCGCGCCCGCGGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTAT                                                                      <  SRR4302547.2343978/94‑1 (MQ=60)
                       CAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCTCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTAT                                                                      >  SRR4302547.4594722/1‑94 (MQ=60)
                       CAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTAT                                                                      <  SRR4302547.5219958/94‑1 (MQ=60)
                        AAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCCACCGATCGATATCCGTTTGGTTTATT                                                                     >  SRR4302547.3407230/1‑94 (MQ=60)
                        AAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATT                                                                     <  SRR4302547.6582025/94‑1 (MQ=60)
                         AAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTT                                                                    <  SRR4302547.6704688/94‑1 (MQ=60)
                         AAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTT                                                                    <  SRR4302547.2940999/94‑1 (MQ=60)
                         AAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTT                                                                    <  SRR4302547.1068856/94‑1 (MQ=60)
                          AGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTT                                                                   <  SRR4302547.2928745/94‑1 (MQ=60)
                           GCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTG                                                                  <  SRR4302547.3404027/94‑1 (MQ=60)
                           GCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTG                                                                  <  SRR4302547.2007557/94‑1 (MQ=60)
                            CGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGT                                                                 >  SRR4302547.2131193/1‑94 (MQ=60)
                            CGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGC                                                                 <  SRR4302547.4837415/94‑1 (MQ=60)
                             GTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCT                                                                >  SRR4302547.3334399/1‑94 (MQ=60)
                              TAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTC                                                               >  SRR4302547.6729186/1‑94 (MQ=60)
                                GGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCAT                                                             <  SRR4302547.1728091/94‑1 (MQ=60)
                                GGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCAT                                                             <  SRR4302547.5692013/94‑1 (MQ=60)
                                 GCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATC                                                            >  SRR4302547.55168/1‑94 (MQ=60)
                                  CGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCA                                                           <  SRR4302547.2605832/94‑1 (MQ=60)
                                   GAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCAT                                                          <  SRR4302547.1918432/94‑1 (MQ=60)
                                   GAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCAT                                                          >  SRR4302547.825983/1‑94 (MQ=60)
                                    AGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATA                                                         <  SRR4302547.717216/94‑1 (MQ=60)
                                      TAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAA                                                       <  SRR4302547.6676413/94‑1 (MQ=60)
                                       AATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAG                                                      <  SRR4302547.1320323/94‑1 (MQ=60)
                                         TACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCC                                                    >  SRR4302547.4606739/1‑94 (MQ=60)
                                          ACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCC                                                   >  SRR4302547.5730971/1‑94 (MQ=60)
                                             GTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCAT                                                <  SRR4302547.2406887/94‑1 (MQ=60)
                                              TGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATG                                               >  SRR4302547.6684493/1‑94 (MQ=60)
                                                TCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGC                                             >  SRR4302547.2759680/1‑94 (MQ=60)
                                                 CTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCA                                            <  SRR4302547.62664/94‑1 (MQ=60)
                                                 CTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCA                                            >  SRR4302547.2825418/1‑94 (MQ=60)
                                                 CTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCA                                            <  SRR4302547.6127723/94‑1 (MQ=60)
                                                 CTAACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCA                                            <  SRR4302547.5960918/94‑1 (MQ=60)
                                                    CCCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGAT                                         <  SRR4302547.2787276/94‑1 (MQ=60)
                                                    CCCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAACCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGAT                                         <  SRR4302547.4291560/94‑1 (MQ=60)
                                                    ACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGAT                                         <  SRR4302547.5982342/94‑1 (MQ=60)
                                                    ACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGAT                                         <  SRR4302547.3461139/94‑1 (MQ=60)
                                                     CCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATG                                        <  SRR4302547.2914317/94‑1 (MQ=60)
                                                      CGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGA                                       <  SRR4302547.222277/94‑1 (MQ=60)
                                                        TCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACA                                     >  SRR4302547.4176687/1‑94 (MQ=60)
                                                         CGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACAT                                    <  SRR4302547.6761068/94‑1 (MQ=60)
                                                         CGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACAT                                    >  SRR4302547.3571702/1‑94 (MQ=60)
                                                            AATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTT                                 >  SRR4302547.2032972/1‑94 (MQ=60)
                                                            AATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTT                                 >  SRR4302547.1388186/1‑94 (MQ=60)
                                                             ATATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTT                                <  SRR4302547.1407000/94‑1 (MQ=60)
                                                             ATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTT                                <  SRR4302547.5769268/94‑1 (MQ=60)
                                                              TATTGCCGCGCCCGACGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTG                               <  SRR4302547.3810319/94‑1 (MQ=60)
                                                               ATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGG                              >  SRR4302547.6341081/1‑94 (MQ=60)
                                                               ATTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGG                              >  SRR4302547.1538653/1‑94 (MQ=60)
                                                               ATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGG                              >  SRR4302547.2544884/1‑94 (MQ=60)
                                                                TTGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGT                             <  SRR4302547.5552389/94‑1 (MQ=60)
                                                                 TGCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTT                            <  SRR4302547.3019944/94‑1 (MQ=60)
                                                                  GCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTG                           >  SRR4302547.4881888/1‑94 (MQ=60)
                                                                  GCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTG                           <  SRR4302547.5619366/94‑1 (MQ=60)
                                                                  GCCGCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTG                           <  SRR4302547.511323/94‑1 (MQ=60)
                                                                     GCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCT                        <  SRR4302547.6762175/94‑1 (MQ=60)
                                                                     GCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCT                        <  SRR4302547.3630618/94‑1 (MQ=60)
                                                                     GCGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTCGCT                        >  SRR4302547.1623592/1‑94 (MQ=60)
                                                                     GCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCT                        >  SRR4302547.2462261/1‑94 (MQ=60)
                                                                      CGCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTG                       >  SRR4302547.6742267/1‑94 (MQ=60)
                                                                       GCCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTTCTCATCATAAAGACCCATGGCAGATTACATTTTTTGTTGGtttc                      >  SRR4302547.4907952/1‑90 (MQ=60)
                                                                       GCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGC                      >  SRR4302547.1414845/1‑94 (MQ=60)
                                                                        CCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCA                     >  SRR4302547.5992518/1‑94 (MQ=60)
                                                                        CCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCA                     <  SRR4302547.3133486/94‑1 (MQ=60)
                                                                        CCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCA                     >  SRR4302547.248451/1‑94 (MQ=60)
                                                                        CCCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCA                     >  SRR4302547.673153/1‑94 (MQ=60)
                                                                         CCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAG                    >  SRR4302547.4135537/1‑94 (MQ=60)
                                                                         CCGCCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAG                    >  SRR4302547.2999773/1‑94 (MQ=60)
                                                                            CCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAAT                 <  SRR4302547.5106988/94‑1 (MQ=60)
                                                                            CCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAAT                 <  SRR4302547.4506932/94‑1 (MQ=60)
                                                                            CCGACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAAC                 <  SRR4302547.3910440/94‑1 (MQ=60)
                                                                             CGACCAGTTCAATCAACCGAACGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATA                >  SRR4302547.6625614/1‑94 (MQ=60)
                                                                              GACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATAT               >  SRR4302547.4351285/1‑94 (MQ=60)
                                                                              GACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATAT               <  SRR4302547.4133629/94‑1 (MQ=60)
                                                                              GACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATAT               <  SRR4302547.4112227/94‑1 (MQ=60)
                                                                               ACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATA              >  SRR4302547.5043660/1‑94 (MQ=60)
                                                                               ACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATA              >  SRR4302547.609424/1‑94 (MQ=60)
                                                                               ACCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATA              <  SRR4302547.6602793/94‑1 (MQ=60)
                                                                               ACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATA              >  SRR4302547.3225634/1‑94 (MQ=60)
                                                                                CCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATAT             <  SRR4302547.3590568/94‑1 (MQ=60)
                                                                                CCAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATAT             <  SRR4302547.2382673/94‑1 (MQ=60)
                                                                                 CAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATT            >  SRR4302547.151403/1‑94 (MQ=60)
                                                                                 CAGTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATT            >  SRR4302547.2643390/1‑94 (MQ=60)
                                                                                 CAGTTCAATCAGCCGATCGATATCCGTTTGGCTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATT            >  SRR4302547.1219888/1‑94 (MQ=60)
                                                                                 CAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATT            >  SRR4302547.5079193/1‑94 (MQ=60)
                                                                                  AGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTG           <  SRR4302547.1058235/94‑1 (MQ=60)
                                                                                   GTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGC          <  SRR4302547.6328543/94‑1 (MQ=60)
                                                                                   GTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGC          <  SRR4302547.2773934/94‑1 (MQ=60)
                                                                                   GTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGC          <  SRR4302547.3233912/94‑1 (MQ=60)
                                                                                   GTTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGC          >  SRR4302547.1990297/1‑94 (MQ=60)
                                                                                   GTTCAATCAGCCGATCGATATCCGTTTGGTTTATTGTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGTTTGGCTGCAGTATATATTGC          <  SRR4302547.6626042/94‑1 (MQ=60)
                                                                                    TTCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCG         >  SRR4302547.4695918/1‑94 (MQ=60)
                                                                                    TTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCG         >  SRR4302547.4773389/1‑94 (MQ=60)
                                                                                     TCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGC        <  SRR4302547.6291258/94‑1 (MQ=60)
                                                                                     TCAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGC        <  SRR4302547.4610269/94‑1 (MQ=60)
                                                                                      CAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCG       <  SRR4302547.3818162/94‑1 (MQ=60)
                                                                                      CAATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAACCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCG       <  SRR4302547.1796289/94‑1 (MQ=60)
                                                                                      CAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCG       >  SRR4302547.4092966/1‑94 (MQ=60)
                                                                                       AATCAGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCGG      <  SRR4302547.5540894/94‑1 (MQ=60)
                                                                                         TCAGCCGATCGATATCCGCTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTAGTTGGCTGCAGAATATATTGCTCGGAA    >  SRR4302547.5901682/1‑94 (MQ=60)
                                                                                          CAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCGGAAA   >  SRR4302547.5851778/1‑94 (MQ=60)
                                                                                           AGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCGGAAAA  <  SRR4302547.2468453/94‑1 (MQ=60)
                                                                                           AGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCGGAAAA  <  SRR4302547.1157101/94‑1 (MQ=60)
                                                                                           AGCCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCGGAAAA  >  SRR4302547.4104635/1‑94 (MQ=60)
                                                                                            |                                                                                            
GGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAATATATTGCGCGGAAAA  >  NC_000913/4463626‑4463810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑