Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,502,206 A→G E194G (GAG→GGG)  b4279 → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,502,2060AG82.0% 494.3 / 104.6 211E194G (GAG→GGG) b4279putative transport protein
Reads supporting (aligned to +/- strand):  ref base A (24/14);  new base G (94/79);  total (118/93)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01

Only 100 of 215 total aligned reads displayed.

TGAAGTATATGGATGGAGAAACTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTC  >  NC_000913/4502114‑4502297
                                                                                            |                                                                                           
    gTATATGGATGGAGAAACTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTgg                                                                                         >  1:3555393/1‑93 (MQ=255)
     tataTGGATGGAGAAACTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGTATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGa                                                                                        >  2:6258493/1‑93 (MQ=255)
     tataTGGATGGAGAAACTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGa                                                                                        <  1:603546/93‑1 (MQ=255)
       tatGGATGGAGAAACTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGAtt                                                                                      >  2:5834555/1‑93 (MQ=255)
       tatGGATGGAGAAACTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGAtt                                                                                      >  2:4345317/1‑93 (MQ=255)
       tatGGATGGAGAAACTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGAtt                                                                                      <  2:5203659/93‑1 (MQ=255)
             tggaGAAACTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGAt                                                                                <  2:4076662/93‑1 (MQ=255)
              ggaGAAACTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATa                                                                               <  2:4244590/93‑1 (MQ=255)
               gagaAACTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATaa                                                                              >  2:4398040/1‑93 (MQ=255)
               gagaAACTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATaa                                                                              <  2:3036999/93‑1 (MQ=255)
               gagaAACTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATaa                                                                              >  1:4418939/1‑93 (MQ=255)
               gagaAACTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATaa                                                                              >  1:695687/1‑93 (MQ=255)
                 gaAACTCTTTTTTTTTAGGCTTGTTCCCAGTTTTACTAGTTCTTTGGTTCAGAAAACGTGTTCCGGAAAGCCAGGGGGGGATTGAAGATaaca                                                                            >  2:2405697/1‑91 (MQ=255)
                   aaCTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAatat                                                                          >  1:5766049/1‑93 (MQ=255)
                   aaCTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAatat                                                                          >  1:153251/1‑93 (MQ=255)
                    aCTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAAtata                                                                         >  2:3815802/1‑93 (MQ=255)
                    aCTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAAtata                                                                         >  1:6539088/1‑93 (MQ=255)
                     ctctTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATaa                                                                        <  1:2534943/93‑1 (MQ=255)
                      tctTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAg                                                                       <  2:4130724/93‑1 (MQ=255)
                      tctTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAg                                                                       <  2:860955/93‑1 (MQ=255)
                      tctTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAg                                                                       >  1:1280747/1‑93 (MQ=255)
                      tctTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAg                                                                       >  2:3287860/1‑93 (MQ=255)
                       ctTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAgg                                                                      <  1:3816065/93‑1 (MQ=255)
                       ctTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAgg                                                                      >  1:5285274/1‑93 (MQ=255)
                        tttttttATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGa                                                                     >  1:6221901/1‑93 (MQ=255)
                        tttttttATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGa                                                                     <  2:5358834/93‑1 (MQ=255)
                        tttttttATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGa                                                                     <  2:4444592/93‑1 (MQ=255)
                         ttttttATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGAt                                                                    <  1:4908965/93‑1 (MQ=255)
                           ttttATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATaa                                                                  >  2:4740478/1‑93 (MQ=255)
                           ttttATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATaa                                                                  >  1:1624204/1‑93 (MQ=255)
                             ttATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAAt                                                                <  2:1026529/93‑1 (MQ=255)
                             ttATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAAt                                                                <  2:2167885/93‑1 (MQ=255)
                              tataGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATc                                                               <  2:3397420/93‑1 (MQ=255)
                              tataGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATc                                                               >  1:3755547/1‑93 (MQ=255)
                               ataGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCa                                                              <  2:5064958/93‑1 (MQ=255)
                                taGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCaa                                                             <  2:6608582/93‑1 (MQ=255)
                                 aGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAAc                                                            <  1:989736/93‑1 (MQ=255)
                                   gCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACAt                                                          <  1:636394/93‑1 (MQ=255)
                                   gCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACAt                                                          <  1:5462560/93‑1 (MQ=255)
                                     cTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACAttt                                                        >  1:6247933/1‑93 (MQ=255)
                                      tGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACAtttt                                                       >  2:5019418/1‑93 (MQ=255)
                                      tGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACAtttt                                                       >  2:2311695/1‑93 (MQ=255)
                                      tGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACAtttt                                                       <  1:4976014/93‑1 (MQ=255)
                                       gTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACAttttt                                                      <  2:2957264/93‑1 (MQ=255)
                                        ttACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATGAATATAAGGATAAATAAACATTTTtg                                                     >  1:1183553/1‑93 (MQ=255)
                                        ttACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTtg                                                     <  1:2823240/93‑1 (MQ=255)
                                        ttACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTtg                                                     <  2:170630/93‑1 (MQ=255)
                                        ttACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTtg                                                     >  1:5331684/1‑93 (MQ=255)
                                         tACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGAGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTtgt                                                    >  1:170591/1‑93 (MQ=255)
                                          aCCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTtgtc                                                   <  1:1462370/93‑1 (MQ=255)
                                           ccAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTtgtct                                                  <  2:1340151/93‑1 (MQ=255)
                                           ccAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTtgtct                                                  <  2:208440/93‑1 (MQ=255)
                                              gTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTtgtctgtc                                               >  1:4816150/1‑93 (MQ=255)
                                               ttttACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTgtctgtct                                              >  1:1765541/1‑93 (MQ=255)
                                               ttttACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTgtctgtct                                              <  2:530545/93‑1 (MQ=255)
                                               ttttACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTgtctgtct                                              <  1:2070001/93‑1 (MQ=255)
                                                tttACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCtt                                             >  2:5705646/1‑93 (MQ=255)
                                                 ttACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTc                                            >  1:3992294/1‑93 (MQ=255)
                                                  tACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCa                                           <  2:4848998/93‑1 (MQ=255)
                                                   aCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAg                                          <  2:6187113/93‑1 (MQ=255)
                                                   aCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACCTTTTTGTCTGTCTTCaa                                          >  2:3111982/1‑92 (MQ=255)
                                                    cTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGa                                         <  1:1103957/93‑1 (MQ=255)
                                                     tAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGaa                                        >  1:3422958/1‑93 (MQ=255)
                                                      aGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGaaa                                       >  2:6389274/1‑93 (MQ=255)
                                                           tttGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACcac                                  >  2:3649238/1‑93 (MQ=255)
                                                           tttGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACcac                                  >  1:4065822/1‑93 (MQ=255)
                                                            ttGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACcaca                                 <  2:428985/93‑1 (MQ=255)
                                                             tGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACAt                                >  2:12412/1‑93 (MQ=255)
                                                              ggATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATc                               <  1:3203177/93‑1 (MQ=255)
                                                               gATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCt                              >  1:5164803/1‑93 (MQ=255)
                                                                aTCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCtt                             >  2:3537405/1‑93 (MQ=255)
                                                                aTCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAAACACATCtt                             >  1:2463363/1‑93 (MQ=255)
                                                                 tCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCttt                            >  2:5593960/1‑93 (MQ=255)
                                                                  cAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTc                           <  1:187900/93‑1 (MQ=255)
                                                                  cAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTc                           <  2:1460709/93‑1 (MQ=255)
                                                                    gAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCaa                         >  1:1002362/1‑93 (MQ=255)
                                                                        aaGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAAtctc                     <  1:5834555/93‑1 (MQ=255)
                                                                        aaGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAAtctc                     <  2:3573157/93‑1 (MQ=255)
                                                                        aaGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAAtctc                     >  2:5543105/1‑93 (MQ=255)
                                                                          gTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTa                   >  1:905977/1‑93 (MQ=255)
                                                                          gTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTa                   >  1:6582124/1‑93 (MQ=255)
                                                                          gTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTa                   <  2:2790369/93‑1 (MQ=255)
                                                                            gCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATg                 <  2:3490886/93‑1 (MQ=255)
                                                                             cTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGa                >  1:6343169/1‑93 (MQ=255)
                                                                             cTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGa                <  1:5550198/93‑1 (MQ=255)
                                                                              tCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGAt               >  2:945310/1‑93 (MQ=255)
                                                                              tCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGAt               >  1:219266/1‑93 (MQ=255)
                                                                               ccAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATc              >  1:2331343/1‑93 (MQ=255)
                                                                                cAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCg             <  1:5243937/93‑1 (MQ=255)
                                                                                 aGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGt            <  1:2889724/93‑1 (MQ=255)
                                                                                  gAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGtt           >  1:4717820/1‑93 (MQ=255)
                                                                                  gAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGtt           >  2:3713984/1‑93 (MQ=255)
                                                                                  gAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGtt           >  2:159318/1‑93 (MQ=255)
                                                                                  gAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGtt           <  2:1731525/93‑1 (MQ=255)
                                                                                  gAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGtt           >  2:4858251/1‑93 (MQ=255)
                                                                                   aaaGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGttt          >  2:3253767/1‑93 (MQ=255)
                                                                                    aaGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGtttt         >  1:5881284/1‑93 (MQ=255)
                                                                                      gTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTcc       >  1:265414/1‑93 (MQ=255)
                                                                                      gTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTcc       >  2:1796448/1‑93 (MQ=255)
                                                                                        cAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTc     <  2:5374003/93‑1 (MQ=255)
                                                                                            |                                                                                           
TGAAGTATATGGATGGAGAAACTCTTTTTTTATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTC  >  NC_000913/4502114‑4502297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGAAGTATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG  >  NC_000913/4502113‑4502299
                                                                                              |                                                                                             
CTGAAGTATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGG                                                                                               <  SRR4302547.3237401/94‑1 (MQ=60)
CTGAAGTATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGG                                                                                               <  SRR4302547.3700186/94‑1 (MQ=60)
 TGAAGTATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGG                                                                                              <  SRR4302547.6681730/94‑1 (MQ=60)
 TGAAGTATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGG                                                                                              >  SRR4302547.4441892/1‑94 (MQ=60)
 TGAAGTATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGG                                                                                              <  SRR4302547.773680/94‑1 (MQ=60)
 TGAAGTATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGAGCTCCAGAAAGTCAGGGG                                                                                              >  SRR4302547.5901318/1‑94 (MQ=60)
  GAAGTATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGT                                                                                             <  SRR4302547.2025868/94‑1 (MQ=60)
   AAGTATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTG                                                                                            >  SRR4302547.4815226/1‑94 (MQ=60)
    AGTATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGG                                                                                           >  SRR4302547.3627949/1‑94 (MQ=60)
      TATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGAT                                                                                         <  SRR4302547.4329863/94‑1 (MQ=60)
      TATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGAT                                                                                         <  SRR4302547.614744/94‑1 (MQ=60)
       ATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATT                                                                                        >  SRR4302547.2571890/1‑94 (MQ=60)
       ATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATT                                                                                        <  SRR4302547.1340376/94‑1 (MQ=60)
       ATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATT                                                                                        >  SRR4302547.5979036/1‑94 (MQ=60)
           GGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAG                                                                                    <  SRR4302547.2933689/94‑1 (MQ=60)
             ATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGAT                                                                                  <  SRR4302547.3502384/94‑1 (MQ=60)
               GGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAA                                                                                >  SRR4302547.4510146/1‑94 (MQ=60)
               GGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAA                                                                                >  SRR4302547.708537/1‑94 (MQ=60)
                GAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAA                                                                               <  SRR4302547.2113006/94‑1 (MQ=60)
                 AGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAAT                                                                              >  SRR4302547.3070861/1‑94 (MQ=60)
                  GAAACTCTTTTTTTAATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAAT                                                                              <  SRR4302547.6082816/94‑1 (MQ=60)
                   AAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATAT                                                                            >  SRR4302547.156044/1‑94 (MQ=60)
                   AAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATAT                                                                            >  SRR4302547.5885298/1‑94 (MQ=60)
                    AACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATA                                                                           <  SRR4302547.2445907/94‑1 (MQ=60)
                    AACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATA                                                                           >  SRR4302547.6675162/1‑94 (MQ=60)
                      CTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAG                                                                         >  SRR4302547.1306531/1‑94 (MQ=60)
                      CTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAG                                                                         <  SRR4302547.2586171/94‑1 (MQ=60)
                      CTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAG                                                                         <  SRR4302547.2575634/94‑1 (MQ=60)
                       TCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGG                                                                        <  SRR4302547.4894043/94‑1 (MQ=60)
                       TCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGG                                                                        >  SRR4302547.5353349/1‑94 (MQ=60)
                       TCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGG                                                                        >  SRR4302547.5394290/1‑94 (MQ=60)
                        CTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGA                                                                       <  SRR4302547.3893820/94‑1 (MQ=60)
                        CTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGA                                                                       >  SRR4302547.6351109/1‑94 (MQ=60)
                         TTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGAT                                                                      <  SRR4302547.538514/94‑1 (MQ=60)
                          TTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATA                                                                     <  SRR4302547.5010626/94‑1 (MQ=60)
                           TTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAA                                                                    >  SRR4302547.1656339/1‑94 (MQ=60)
                           TTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAA                                                                    <  SRR4302547.5624349/94‑1 (MQ=60)
                            TTTT‑ATAGGCCTGTTACCAGTTTTTCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAA                                                                   >  SRR4302547.646701/1‑94 (MQ=60)
                             TTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAAT                                                                  >  SRR4302547.5548218/1‑94 (MQ=60)
                             TTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAAT                                                                  <  SRR4302547.1169736/94‑1 (MQ=60)
                              TT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATC                                                                 >  SRR4302547.3832172/1‑94 (MQ=60)
                                   AGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACA                                                             <  SRR4302547.1009004/94‑1 (MQ=60)
                                   AGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACA                                                             >  SRR4302547.1264991/1‑94 (MQ=60)
                                     GCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATT                                                           <  SRR4302547.5575401/94‑1 (MQ=60)
                                     GCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATT                                                           <  SRR4302547.648147/94‑1 (MQ=60)
                                      CCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTT                                                          >  SRR4302547.6377722/1‑94 (MQ=60)
                                       CTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTT                                                         >  SRR4302547.6311638/1‑94 (MQ=60)
                                        TGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTT                                                        <  SRR4302547.5078948/94‑1 (MQ=60)
                                         GTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATGAATATAAGGATAAATAAACATTTTTG                                                       >  SRR4302547.1207457/1‑94 (MQ=60)
                                         GTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTG                                                       >  SRR4302547.5441572/1‑94 (MQ=60)
                                          TTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGT                                                      <  SRR4302547.2879712/94‑1 (MQ=60)
                                          TTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGAGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGT                                                      >  SRR4302547.173710/1‑94 (MQ=60)
                                           TACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTC                                                     <  SRR4302547.3434263/94‑1 (MQ=60)
                                            ACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCT                                                    <  SRR4302547.1491594/94‑1 (MQ=60)
                                             CCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTG                                                   >  SRR4302547.6693857/1‑94 (MQ=60)
                                             CCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTG                                                   >  SRR4302547.1560265/1‑94 (MQ=60)
                                             CCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTG                                                   <  SRR4302547.6569184/94‑1 (MQ=60)
                                               AGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTC                                                 >  SRR4302547.866253/1‑94 (MQ=60)
                                               AGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTC                                                 >  SRR4302547.4915961/1‑94 (MQ=60)
                                                GTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCT                                                >  SRR4302547.1800562/1‑94 (MQ=60)
                                                GTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCT                                                >  SRR4302547.6359883/1‑94 (MQ=60)
                                                 TTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTT                                               <  SRR4302547.2111322/94‑1 (MQ=60)
                                                  TTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTC                                              >  SRR4302547.4073466/1‑94 (MQ=60)
                                                    TACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAG                                            <  SRR4302547.617600/94‑1 (MQ=60)
                                                    TACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAG                                            <  SRR4302547.5015553/94‑1 (MQ=60)
                                                    TACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAG                                            <  SRR4302547.2728243/94‑1 (MQ=60)
                                                    TACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAG                                            >  SRR4302547.4835319/1‑94 (MQ=60)
                                                     ACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGA                                           >  SRR4302547.1862198/1‑94 (MQ=60)
                                                      CTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAA                                          <  SRR4302547.1126155/94‑1 (MQ=60)
                                                      CTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAA                                          >  SRR4302547.3492653/1‑94 (MQ=60)
                                                       TAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAA                                         >  SRR4302547.1022307/1‑94 (MQ=60)
                                                           TCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCA                                     >  SRR4302547.2308519/1‑94 (MQ=60)
                                                           TCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCA                                     >  SRR4302547.4078181/1‑94 (MQ=60)
                                                            CTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCAC                                    >  SRR4302547.4148401/1‑94 (MQ=60)
                                                               TGTATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGAATTGAAGATAAATATAAGGATAAATCAGCATTTTTGTCTGTCTTCAGAAAACCACATC                                 <  SRR4302547.5473965/94‑1 (MQ=60)
                                                                GGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCT                                <  SRR4302547.3267060/94‑1 (MQ=60)
                                                                GGATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCT                                >  SRR4302547.5271143/1‑94 (MQ=60)
                                                                 GATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTT                               >  SRR4302547.5141513/1‑94 (MQ=60)
                                                                 GATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAAACACATCTT                               >  SRR4302547.2513157/1‑94 (MQ=60)
                                                                  ATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTT                              >  SRR4302547.3860011/1‑94 (MQ=60)
                                                                  ATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTT                              >  SRR4302547.2588183/1‑94 (MQ=60)
                                                                  ATCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTT                              >  SRR4302547.3767308/1‑94 (MQ=60)
                                                                   TCAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTC                             <  SRR4302547.5303449/94‑1 (MQ=60)
                                                                    CAGAAAAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCA                            <  SRR4302547.191442/94‑1 (MQ=60)
                                                                     AGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAA                           >  SRR4302547.1021917/1‑94 (MQ=60)
                                                                         AAAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTC                       >  SRR4302547.5937987/1‑94 (MQ=60)
                                                                          AAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCT                      <  SRR4302547.5955274/94‑1 (MQ=60)
                                                                          AAGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAAACACATCTTTCAATCTCT                      >  SRR4302547.638478/1‑94 (MQ=60)
                                                                           AGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTA                     >  SRR4302547.923436/1‑94 (MQ=60)
                                                                           AGTGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTA                     >  SRR4302547.6719192/1‑94 (MQ=60)
                                                                             TGCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATG                   <  SRR4302547.5802946/94‑1 (MQ=60)
                                                                              GCTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGA                  >  SRR4302547.6475268/1‑94 (MQ=60)
                                                                               CTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGAT                 <  SRR4302547.5664713/94‑1 (MQ=60)
                                                                               CTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGAT                 >  SRR4302547.1994317/1‑94 (MQ=60)
                                                                               CTCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGAT                 >  SRR4302547.223394/1‑94 (MQ=60)
                                                                                TCCAGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATC                >  SRR4302547.3922265/1‑94 (MQ=60)
                                                                                TCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATC                >  SRR4302547.2378599/1‑94 (MQ=60)
                                                                                  CAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGT              <  SRR4302547.5351869/94‑1 (MQ=60)
                                                                                   AGAAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTT             >  SRR4302547.4815517/1‑94 (MQ=60)
                                                                                   AGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTT             <  SRR4302547.2947364/94‑1 (MQ=60)
                                                                                     AAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTT           >  SRR4302547.6003052/1‑94 (MQ=60)
                                                                                     AAAGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTT           <  SRR4302547.960448/94‑1 (MQ=60)
                                                                                       AGTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCC         >  SRR4302547.270378/1‑94 (MQ=60)
                                                                                        GTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCT        <  SRR4302547.704875/94‑1 (MQ=60)
                                                                                        GTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCT        >  SRR4302547.1594298/1‑94 (MQ=60)
                                                                                        GTCAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCT        >  SRR4302547.885784/1‑94 (MQ=60)
                                                                                          CAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCG      >  SRR4302547.3613474/1‑94 (MQ=60)
                                                                                          CAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCG      <  SRR4302547.1752043/94‑1 (MQ=60)
                                                                                          CAGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCG      <  SRR4302547.1712782/94‑1 (MQ=60)
                                                                                           AGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGT     >  SRR4302547.6562436/1‑94 (MQ=60)
                                                                                           AGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGT     >  SRR4302547.3859607/1‑94 (MQ=60)
                                                                                           AGGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGT     >  SRR4302547.6246643/1‑94 (MQ=60)
                                                                                           AGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGT     >  SRR4302547.4980813/1‑94 (MQ=60)
                                                                                            GGGGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTC    >  SRR4302547.4318933/1‑94 (MQ=60)
                                                                                             GAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCT   <  SRR4302547.1659571/94‑1 (MQ=60)
                                                                                              GGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG  <  SRR4302547.3150079/94‑1 (MQ=60)
                                                                                              GGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG  >  SRR4302547.4587078/1‑94 (MQ=60)
                                                                                              AGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG  >  SRR4302547.5615515/1‑94 (MQ=60)
                                                                                              |                                                                                             
CTGAAGTATATGGATGGAGAAACTCTTTTTTT‑ATAGGCCTGTTACCAGTTTTACTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG  >  NC_000913/4502113‑4502299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑