Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
343 |
268.7 |
10873516 |
97.0% |
10547310 |
150.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP009974 |
1,920,754 |
1 bp→TTT |
intergenic (‑383/+172) |
RPPX_08560 ← / ← RPPX_08565 |
16S ribosomal RNA/CoA‑transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP009974 | 1,920,753 | 1 | . | T | 86.7%
| 11.7
/ 10.6
| 30 | intergenic (‑382/+173) | RPPX_08560/RPPX_08565 | 16S ribosomal RNA/CoA‑transferase |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base T (11/15); total (12/18) |
| Fisher's exact test for biased strand distribution p-value = 6.32e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 |
| * | CP009974 | 1,920,753 | 2 | . | T | 86.7%
| 11.7
/ 10.6
| 30 | intergenic (‑382/+173) | RPPX_08560/RPPX_08565 | 16S ribosomal RNA/CoA‑transferase |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base T (11/15); total (12/18) |
| Fisher's exact test for biased strand distribution p-value = 6.32e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| * | CP009974 | 1,920,754 | 0 | A | T | 100.0%
| 14.0
/ NA
| 30 | intergenic (‑383/+172) | RPPX_08560/RPPX_08565 | 16S ribosomal RNA/CoA‑transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (12/18); total (12/18) |
CTTGGAAGCGCTGTCAACCATCATCTCAACCGCTTTCGATCATTTGATCGTAGCCCTTACAGC‑‑A‑‑CCTTCCGAATCGTCTAACTCATTGAATCTCAAGGAGTTTGTCGTTCCGTTGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCA > CP009974/1920691‑1920863
|||||
cTTGGAAGCGCTGTCAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TACCTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCgag < 1:2243879/151‑1 (MQ=11)
ttGGAAGCGCTGTCAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCgaga > 2:4716883/1‑151 (MQ=2)
ttGGAAGCGCTGTCAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCgaga < 1:3606830/151‑1 (MQ=2)
ggAAGCGCTGTCAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGACGTGGGGCGCATTATACGGGGATTCGAGAgg > 2:4451016/1‑151 (MQ=2)
ggAAGCGCTGTCAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAgg < 2:1026015/151‑1 (MQ=2)
ggAAGCGCTGTCAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGC‑‑TTTCCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAgg < 2:3060508/151‑1 (MQ=2)
gAAGCGCTGTCAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTTGAGAggg > 1:2175135/1‑151 (MQ=2)
gAAGCGCTGTCAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATGATAAGGGGATTGGAGCggg > 1:2175116/1‑151 (MQ=2)
aGCGCTGTCAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCg > 1:974192/1‑151 (MQ=2)
aGCGCTGTCAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTGTCGTTCCGTTGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCg < 1:1857849/151‑1 (MQ=11)
gcTGTCAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCa > 2:4412377/1‑151 (MQ=2)
gcTGTCAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCa < 2:1156168/151‑1 (MQ=2)
tGTCAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTCG‑CTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAAc < 2:923169/151‑1 (MQ=2)
gTCCACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGC‑‑TTTCCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAAcc < 2:1789953/151‑1 (MQ=2)
cAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCtt > 1:4597702/1‑151 (MQ=2)
cAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCtt < 2:1009439/151‑1 (MQ=2)
cAACCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGC‑‑TTTCCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCtt > 2:4650512/1‑151 (MQ=2)
aaCCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TATCTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCttt < 2:3484699/151‑1 (MQ=2)
aaCCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCttt < 1:714560/151‑1 (MQ=2)
aaCCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCttt < 1:2547308/151‑1 (MQ=2)
aaCCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCttt < 2:461927/151‑1 (MQ=11)
aaCCATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCttt < 2:5227266/151‑1 (MQ=2)
ccATCTTCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTaa < 1:4357038/151‑1 (MQ=2)
ccATCATCTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTaa > 1:972799/1‑151 (MQ=2)
tcatcTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAAttt < 2:2474804/151‑1 (MQ=2)
tcatcTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAAttt > 1:4022223/1‑151 (MQ=2)
tcatcTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGC‑‑TTTCCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAAttt < 2:3429108/151‑1 (MQ=2)
catcTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTc < 1:415163/151‑1 (MQ=2)
catcTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTc > 1:5039975/1‑151 (MQ=2)
atcTCAACCGCTTTCGATCATTCGACCGTAGCCCCTCCAGCTTT‑‑CCTTTC‑ACTACT‑TAACTCATTGAATCTCAAGGAGCTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATCATAACGCGATTCGCTATGGCGTCAACCTTTAATTTCa > 2:3751597/1‑151 (MQ=2)
|||||
CTTGGAAGCGCTGTCAACCATCATCTCAACCGCTTTCGATCATTTGATCGTAGCCCTTACAGC‑‑A‑‑CCTTCCGAATCGTCTAACTCATTGAATCTCAAGGAGTTTGTCGTTCCGTTGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCA > CP009974/1920691‑1920863
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
