Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
336 |
367.5 |
14491858 |
96.8% |
14028118 |
150.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP009974 |
4,031,017 |
Δ1 bp |
intergenic (+159/‑625) |
RPPX_17905 → / → RPPX_17915 |
23S rRNA methyltransferase/sulfurtransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP009974 | 4,031,017 | 0 | A | . | 93.5%
| 128.5
/ 6.9
| 46 | intergenic (+159/‑625) | RPPX_17905/RPPX_17915 | 23S rRNA methyltransferase/sulfurtransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/3); new base . (20/23); total (20/26) |
| Fisher's exact test for biased strand distribution p-value = 2.46e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAG > CP009974/4030871‑4031166
|
gtTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCTCTG‑GGccc > 1:1282355/1‑151 (MQ=255)
ttctgcttctgcttctgcttctgcttctgcttctgcTTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGcccc > 1:4821327/1‑151 (MQ=12)
tctgcttctgcttctgcttctgcttctgcttctgctTTTGCTTCTCAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCa < 1:1345558/151‑1 (MQ=255)
tctgcttctgcttctgcttctgcttctgcttctgctTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCa > 1:3838624/1‑151 (MQ=16)
tctgcttctgcttctgcttctgcttctgcttctgctTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCa > 1:4050422/1‑151 (MQ=255)
ctgcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAg < 2:7047301/151‑1 (MQ=16)
ctgcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAg < 1:634149/151‑1 (MQ=255)
ctgcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAg < 1:3742304/151‑1 (MQ=255)
tgcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGATTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAgg > 1:5693036/1‑151 (MQ=12)
tgcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAgg > 2:1032245/1‑151 (MQ=16)
tgcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAgg < 2:1965994/151‑1 (MQ=16)
tgcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAgg < 1:5614748/151‑1 (MQ=255)
gcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGa > 2:267482/1‑151 (MQ=16)
cttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGAt < 2:2990654/151‑1 (MQ=255)
ttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATg < 1:5335912/151‑1 (MQ=16)
ttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATg > 2:1482426/1‑151 (MQ=255)
tctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATgg < 1:4694812/151‑1 (MQ=255)
tctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATgg > 2:1387356/1‑151 (MQ=16)
tctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATgg > 2:1386478/1‑151 (MQ=16)
ctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATggg > 1:4568490/1‑151 (MQ=12)
ctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATggg < 1:1993146/151‑1 (MQ=255)
ctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATggg > 1:1142239/1‑151 (MQ=255)
tgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATgggg > 2:7068625/1‑151 (MQ=19)
gcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGc > 2:2366530/1‑151 (MQ=255)
gcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGc < 2:1509729/151‑1 (MQ=16)
ctgctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGcc < 1:6921584/148‑1 (MQ=255)
ttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGGTGGGGCCt < 1:2891353/151‑1 (MQ=255)
ttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCt < 1:937047/151‑1 (MQ=255)
ttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCt > 2:4440014/1‑151 (MQ=19)
tctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtg > 2:560315/1‑150 (MQ=16)
tctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtg < 2:2066250/151‑2 (MQ=16)
tctgcttctgcttccgcctctgctgTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtg < 2:3791108/151‑2 (MQ=11)
ctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgc < 1:2515308/151‑3 (MQ=9)
ctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgc < 2:5374913/151‑3 (MQ=12)
ttcttcttcttttgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgca > 2:4569028/3‑148 (MQ=11)
tgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgca > 1:5687093/1‑148 (MQ=9)
tgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgca < 2:84170/151‑4 (MQ=2)
cttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcagc < 1:1935640/151‑6 (MQ=2)
cttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcagc < 1:1934493/151‑6 (MQ=9)
cttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcagc < 2:769327/151‑6 (MQ=9)
ttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcagcg > 2:3509149/1‑145 (MQ=255)
ctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCTGCcgcgcg > 2:3735778/1‑151 (MQ=255)
gcttTTGCTTCTGAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGc < 2:4876741/151‑1 (MQ=1)
gCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGtt < 2:3415483/151‑1 (MQ=31)
gAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGGCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAg < 1:4708733/151‑1 (MQ=11)
gAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAg < 1:4217361/151‑1 (MQ=11)
|
GTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAG > CP009974/4030871‑4031166
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
