Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 149,697 G→T 21.8% I739I (ATC→ATA)  htrE ← putative outer membrane usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273149,6970GT21.8% 690.9 / 136.2 252I739I (ATC→ATA) htrEputative outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base G (94/103);  new base T (22/33);  total (116/136)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 259 total aligned reads displayed.

ACCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTAAAACTATCATTACT  >  CP009273/149550‑149846
                                                                                                                                                   |                                                                                                                                                     
aCCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGAt                                                                                                                                                     >  1:1217412/1‑150 (MQ=255)
aCCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGAt                                                                                                                                                     >  1:193398/1‑150 (MQ=255)
 ccATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTCCTTTTTAATTCAACATCGGTCTCAAGATCGTTGATATCCAGCGCGATa                                                                                                                                                    >  2:3055859/1‑150 (MQ=255)
   aTCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACg                                                                                                                                                  >  1:1541153/1‑150 (MQ=255)
   aTCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACg                                                                                                                                                  >  1:534808/1‑150 (MQ=255)
        ttCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAATATCGTTGATATCCAGCGCGATACGGtttt                                                                                                                                             >  1:1859439/1‑150 (MQ=255)
        ttCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGtttt                                                                                                                                             >  2:2399666/1‑150 (MQ=255)
          cGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCa                                                                                                                                           <  2:4390312/150‑1 (MQ=255)
          cGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCa                                                                                                                                           <  2:2686181/150‑1 (MQ=255)
          cGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCa                                                                                                                                           <  1:2750264/150‑1 (MQ=255)
           gtgtGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCAt                                                                                                                                          >  2:1379909/1‑150 (MQ=255)
            tgtgATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATg                                                                                                                                         >  1:1100082/1‑150 (MQ=255)
               gATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCATtata                                                                                                                                      <  1:1666404/150‑1 (MQ=255)
               gATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCATtata                                                                                                                                      <  1:1666262/150‑1 (MQ=255)
               gATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCATGATa                                                                                                                                      >  1:740365/1‑150 (MQ=255)
               gATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATa                                                                                                                                      <  2:2726676/150‑1 (MQ=255)
                  gTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAgg                                                                                                                                   >  2:3703294/1‑150 (MQ=255)
                    tCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGgag                                                                                                                                 >  1:2109103/1‑150 (MQ=255)
                     cATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGgaga                                                                                                                                >  2:2152535/1‑150 (MQ=255)
                           ggCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAgc                                                                                                                          <  1:1848136/150‑1 (MQ=255)
                             cTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAgcgc                                                                                                                        >  1:2612552/1‑150 (MQ=255)
                               gATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCATTATAAGGAGAAAGAGCGCTg                                                                                                                      <  1:1792147/150‑1 (MQ=255)
                                 ttGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGt                                                                                                                    <  2:1109172/150‑1 (MQ=255)
                                       ttGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACAcc                                                                                                              >  2:900040/1‑150 (MQ=255)
                                       ttGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACAcc                                                                                                              >  1:1178976/1‑150 (MQ=255)
                                        tGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCa                                                                                                             >  1:246059/1‑150 (MQ=255)
                                         gCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGTTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCTCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCAt                                                                                                            <  2:955029/150‑1 (MQ=255)
                                         gCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCAt                                                                                                            <  1:2155443/150‑1 (MQ=255)
                                         gCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCAt                                                                                                            <  2:1836095/150‑1 (MQ=255)
                                             ggTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAAcc                                                                                                        >  2:3922557/1‑150 (MQ=255)
                                              gTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAAccc                                                                                                       >  2:4587080/1‑150 (MQ=255)
                                                ttCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCa                                                                                                     >  1:3595397/1‑150 (MQ=255)
                                                  cAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCatc                                                                                                   <  2:2591581/150‑1 (MQ=255)
                                                   aaaaTCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCatcg                                                                                                  <  2:2323018/150‑1 (MQ=255)
                                                    aaaTCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCatcga                                                                                                 <  1:334289/150‑1 (MQ=255)
                                                    aaaTCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCatcga                                                                                                 >  2:2142116/1‑150 (MQ=255)
                                                    aaaTCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCatcga                                                                                                 >  1:3970653/1‑150 (MQ=255)
                                                     aaTCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCatcgat                                                                                                >  1:3235315/1‑150 (MQ=255)
                                                      aTCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCatcgatc                                                                                               <  1:4098778/150‑1 (MQ=255)
                                                          gCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATa                                                                                           >  1:41513/1‑150 (MQ=255)
                                                           cAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAg                                                                                          >  2:3524139/1‑150 (MQ=255)
                                                            aaaGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGt                                                                                         >  2:3026690/1‑150 (MQ=255)
                                                            aaaGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGt                                                                                         >  2:2483847/1‑150 (MQ=255)
                                                              aGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTAc                                                                                       <  2:2227645/150‑1 (MQ=255)
                                                                   acTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCAAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTg                                                                                  >  2:2061168/1‑150 (MQ=255)
                                                                     tGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGcc                                                                                <  1:2964584/150‑1 (MQ=255)
                                                                      gAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCa                                                                               <  2:1426165/150‑1 (MQ=255)
                                                                       aaCCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCAt                                                                              <  2:1627417/150‑1 (MQ=255)
                                                                         cccTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATaa                                                                            <  2:1906509/150‑1 (MQ=255)
                                                                           cTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAAtt                                                                          >  2:1482501/1‑150 (MQ=255)
                                                                           cTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAAtt                                                                          <  2:1048203/150‑1 (MQ=255)
                                                                           cTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAAtt                                                                          <  2:865770/150‑1 (MQ=255)
                                                                           cTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAAtt                                                                          <  2:1048140/150‑1 (MQ=255)
                                                                             gACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTa                                                                        <  1:3151349/150‑1 (MQ=255)
                                                                                gcgGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCATTATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTc                                                                     <  2:407511/150‑1 (MQ=255)
                                                                                  ggTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTcgc                                                                   <  2:3758339/150‑1 (MQ=255)
                                                                                     aCAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCt                                                                >  1:1291986/1‑150 (MQ=255)
                                                                                     aCAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCt                                                                >  1:3095783/1‑150 (MQ=255)
                                                                                      cAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTc                                                               <  2:3037545/150‑1 (MQ=255)
                                                                                        gCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCt                                                             <  1:3091067/150‑1 (MQ=255)
                                                                                           aCTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGAGAGTACTGTTGCCATAATTTATTCGCGCTCCTTGa                                                          <  2:2095890/150‑1 (MQ=255)
                                                                                            cTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAg                                                         <  1:3940096/150‑1 (MQ=255)
                                                                                              gCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCa                                                       >  2:2179680/1‑150 (MQ=255)
                                                                                               cACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCAc                                                      <  2:3607975/150‑1 (MQ=255)
                                                                                                 cTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCt                                                    <  1:804861/150‑1 (MQ=255)
                                                                                                 cTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCt                                                    >  2:3191620/1‑150 (MQ=255)
                                                                                                 cTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCt                                                    <  2:4246126/150‑1 (MQ=255)
                                                                                                 cTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCt                                                    <  2:2870037/150‑1 (MQ=255)
                                                                                                 cTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGAGAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCt                                                    <  1:3302129/150‑1 (MQ=255)
                                                                                                    gTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGa                                                 <  2:706207/150‑1 (MQ=255)
                                                                                                      aCTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGc                                               >  2:1932532/1‑150 (MQ=255)
                                                                                                          tttAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGa                                           <  1:3482247/150‑1 (MQ=255)
                                                                                                           ttAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGaa                                          <  2:758981/150‑1 (MQ=255)
                                                                                                             aaTTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACt                                        >  2:2885765/1‑150 (MQ=255)
                                                                                                             aaTTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACt                                        >  1:3193948/1‑150 (MQ=255)
                                                                                                                    cATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCa                                 <  2:1178976/150‑1 (MQ=255)
                                                                                                                     aTCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAg                                >  2:193857/1‑150 (MQ=255)
                                                                                                                          tctcAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTAt                           <  2:511594/150‑1 (MQ=255)
                                                                                                                          tctcAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTAt                           <  2:1034233/150‑1 (MQ=255)
                                                                                                                           ctcAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATc                          <  1:4553716/150‑1 (MQ=255)
                                                                                                                           ctcAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATc                          <  1:1372233/150‑1 (MQ=255)
                                                                                                                              aaGATCGTTGATATCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGa                       <  1:3768533/150‑1 (MQ=255)
                                                                                                                              aaGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGa                       <  2:3417213/150‑1 (MQ=255)
                                                                                                                                gATCGTTGATATCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGt                     <  1:1021611/150‑1 (MQ=255)
                                                                                                                                 aTCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTc                    <  1:4341652/150‑1 (MQ=255)
                                                                                                                                     ttGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTa                <  1:2253162/150‑1 (MQ=255)
                                                                                                                                      tGATATCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTaa               >  1:165381/1‑150 (MQ=255)
                                                                                                                                       gATATCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTaaa              >  1:4087446/1‑150 (MQ=255)
                                                                                                                                        atatCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTaaaa             >  2:2560083/1‑150 (MQ=255)
                                                                                                                                         tatCCAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTAAAAc            >  2:433193/1‑150 (MQ=255)
                                                                                                                                          atCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTAAAACt           >  2:2925723/1‑150 (MQ=255)
                                                                                                                                            ccAGCGCTATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTAAAACTAt         <  1:1169385/150‑1 (MQ=255)
                                                                                                                                            ccAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTAAAACTAt         <  2:3118040/150‑1 (MQ=255)
                                                                                                                                            ccAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTAAAACTAt         <  2:3117938/150‑1 (MQ=255)
                                                                                                                                             cAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTAAAACTATc        <  1:2151003/150‑1 (MQ=255)
                                                                                                                                             cAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTAAAACTATc        >  2:3696157/1‑150 (MQ=255)
                                                                                                                                              aGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTAAAACTAtaa       >  2:4575074/1‑148 (MQ=255)
                                                                                                                                               gcgcGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTAAAACTATCAt      <  1:517718/150‑1 (MQ=255)
                                                                                                                                                 gcgATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCCCTAAAACTATCATTa    >  2:1753765/1‑150 (MQ=255)
                                                                                                                                                   gATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTAAAACTATCATTACt  <  1:3029560/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCATAACCCCATCGATCGATAGTACTGTTGCCATAATTTATTCGCGCTCCTTGAGCACCTGGAGCCTGAACTACCGCCAGTGTATCGGAGTCGCTAAAACTATCATTACT  >  CP009273/149550‑149846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A