Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 212,284 G→T 38.5% A177S (GCA→TCA)  nlpE → lipoprotein involved with copper homeostasis and adhesion

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273212,2840GT38.5% 401.0 / 307.7 285A177S (GCA→TCA) nlpElipoprotein involved with copper homeostasis and adhesion
Reads supporting (aligned to +/- strand):  ref base G (97/78);  new base T (45/65);  total (142/143)
Fisher's exact test for biased strand distribution p-value = 2.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

TTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTA  >  CP009273/212135‑212432
                                                                                                                                                     |                                                                                                                                                    
ttGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACg                                                                                                                                                      >  1:1613344/1‑150 (MQ=255)
  gAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCa                                                                                                                                                    >  2:1888723/1‑150 (MQ=255)
   aaTCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCag                                                                                                                                                   >  1:1039064/1‑150 (MQ=255)
   aaTCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCag                                                                                                                                                   <  2:484090/150‑1 (MQ=255)
   aaTCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCag                                                                                                                                                   >  1:2342774/1‑150 (MQ=255)
      cGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGc                                                                                                                                                >  2:2319816/1‑150 (MQ=255)
        cAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTg                                                                                                                                              >  1:1111923/1‑150 (MQ=255)
        cAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTg                                                                                                                                              >  2:4302436/1‑150 (MQ=255)
         aGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTgg                                                                                                                                             <  2:4038441/150‑1 (MQ=255)
         aGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTgg                                                                                                                                             >  1:2802179/1‑150 (MQ=255)
          gTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTGGCGAATAACTCAGAGCTGGa                                                                                                                                            <  1:597736/150‑1 (MQ=255)
          gTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACACTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGa                                                                                                                                            >  2:4124921/1‑150 (MQ=255)
           ttCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAg                                                                                                                                           <  2:3092655/150‑1 (MQ=255)
           ttCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAg                                                                                                                                           >  2:1503207/1‑150 (MQ=255)
            tCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGc                                                                                                                                          >  1:400351/1‑150 (MQ=255)
                cTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAg                                                                                                                                      <  2:1621941/150‑1 (MQ=255)
                  ataCGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCt                                                                                                                                    <  2:426015/150‑1 (MQ=255)
                  ataCGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCt                                                                                                                                    <  2:2955953/150‑1 (MQ=255)
                    aCGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTAc                                                                                                                                  >  1:1463160/1‑150 (MQ=255)
                       cTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTg                                                                                                                               <  2:2283085/150‑1 (MQ=255)
                             gCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTgcg                                                                                                                         <  1:3682579/150‑1 (MQ=255)
                              cGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTgcgc                                                                                                                        <  1:4200316/150‑1 (MQ=255)
                               ggCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTgcgcg                                                                                                                       >  2:3769995/1‑150 (MQ=255)
                                gCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTgcgcgc                                                                                                                      <  2:2653325/150‑1 (MQ=255)
                                  acaATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCgg                                                                                                                    <  1:4640372/150‑1 (MQ=255)
                                    aaTCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTc                                                                                                                  >  2:567761/1‑150 (MQ=255)
                                         aGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGt                                                                                                             <  1:2772375/150‑1 (MQ=255)
                                           tttACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGa                                                                                                           <  2:3962532/150‑1 (MQ=255)
                                              aCCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGaaaa                                                                                                        <  2:243788/150‑1 (MQ=255)
                                               ccTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGaaaaa                                                                                                       <  2:3853135/150‑1 (MQ=255)
                                               ccTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGaaaaa                                                                                                       >  1:780408/1‑150 (MQ=255)
                                                cTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAAc                                                                                                      >  2:4426801/1‑150 (MQ=255)
                                                cTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAAc                                                                                                      >  1:964689/1‑150 (MQ=255)
                                                 tATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAAcc                                                                                                     <  1:2260372/150‑1 (MQ=255)
                                                  aTGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCg                                                                                                    >  2:2022007/1‑150 (MQ=255)
                                                    gACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGgt                                                                                                  >  2:456453/1‑150 (MQ=255)
                                                      cGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGgtgt                                                                                                <  1:1311083/150‑1 (MQ=255)
                                                         cGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTAc                                                                                             <  1:654506/150‑1 (MQ=255)
                                                           aTGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTg                                                                                           >  1:3829024/1‑150 (MQ=255)
                                                            tGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGt                                                                                          <  1:2201255/150‑1 (MQ=255)
                                                                ccTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGt                                                                                      <  1:3372311/150‑1 (MQ=255)
                                                                  tGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAg                                                                                    <  1:4085831/150‑1 (MQ=255)
                                                                  tGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAg                                                                                    >  2:3400998/1‑150 (MQ=255)
                                                                    cGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGaa                                                                                  >  1:2558016/1‑150 (MQ=255)
                                                                      gggCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAgg                                                                                >  1:3421790/1‑150 (MQ=255)
                                                                        gCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTc                                                                              >  2:3659089/1‑150 (MQ=255)
                                                                        gCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTc                                                                              <  1:2326530/150‑1 (MQ=255)
                                                                            gTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACtt                                                                          <  1:86897/150‑1 (MQ=255)
                                                                                  ttATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCt                                                                    <  1:1590427/150‑1 (MQ=255)
                                                                                   tataTGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCtt                                                                   <  2:2863685/150‑1 (MQ=255)
                                                                                   tataTGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCtt                                                                   >  1:3245755/1‑150 (MQ=255)
                                                                                     tatGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGa                                                                 >  1:3293915/1‑150 (MQ=255)
                                                                                     tatGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGa                                                                 <  1:4595757/150‑1 (MQ=255)
                                                                                      atGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAg                                                                <  1:1772635/150‑1 (MQ=255)
                                                                                         gCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGt                                                             <  2:198473/150‑1 (MQ=255)
                                                                                          cTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTa                                                            <  1:1386294/150‑1 (MQ=255)
                                                                                          cTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTa                                                            <  2:2565240/150‑1 (MQ=255)
                                                                                           tGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTaa                                                           >  2:819078/1‑150 (MQ=255)
                                                                                               gcggcgACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCg                                                       >  2:3486459/1‑150 (MQ=255)
                                                                                                  gcgACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGAt                                                    <  2:1613331/150‑1 (MQ=255)
                                                                                                   cgACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATa                                                   >  2:4465624/1‑150 (MQ=255)
                                                                                                   cgACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATa                                                   <  1:1212573/150‑1 (MQ=255)
                                                                                                    gACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATAc                                                  >  2:560654/1‑150 (MQ=255)
                                                                                                      ccTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCg                                                <  2:333532/150‑1 (MQ=255)
                                                                                                       cTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCgg                                               >  2:3733170/1‑150 (MQ=255)
                                                                                                            cTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTgcgc                                          >  2:1801413/1‑150 (MQ=255)
                                                                                                            cTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTgcgc                                          >  2:922201/1‑150 (MQ=255)
                                                                                                             tGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGcc                                         >  1:4231929/1‑150 (MQ=255)
                                                                                                             tGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGcc                                         <  2:2155150/150‑1 (MQ=255)
                                                                                                              gATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCg                                        <  1:4232815/150‑1 (MQ=255)
                                                                                                               aTTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGa                                       >  2:2852630/1‑150 (MQ=255)
                                                                                                               aTTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGa                                       <  1:4474614/150‑1 (MQ=255)
                                                                                                               aTTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGa                                       >  2:1065064/1‑150 (MQ=255)
                                                                                                                 tGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACt                                     >  2:2724184/1‑150 (MQ=255)
                                                                                                                 tGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACt                                     <  1:3087398/150‑1 (MQ=255)
                                                                                                                      gACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGt                                >  2:3493661/1‑150 (MQ=255)
                                                                                                                      gACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGt                                >  2:720815/1‑150 (MQ=255)
                                                                                                                        ccGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTAt                              >  2:1784212/1‑150 (MQ=255)
                                                                                                                        ccGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTAt                              >  2:2745560/1‑150 (MQ=255)
                                                                                                                         cGGAAAACGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTAtt                             <  1:3615543/150‑1 (MQ=255)
                                                                                                                         cGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTAtt                             <  2:1399819/150‑1 (MQ=255)
                                                                                                                           gAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTgg                           >  1:2114314/1‑150 (MQ=255)
                                                                                                                           gAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTgg                           >  1:2955088/1‑150 (MQ=255)
                                                                                                                              aaCGTTTCATGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCAc                        >  2:3183904/1‑150 (MQ=255)
                                                                                                                              aaCGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCAc                        <  2:3116538/150‑1 (MQ=255)
                                                                                                                                cGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCAccc                      <  1:1916390/150‑1 (MQ=255)
                                                                                                                                cGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCAccc                      <  1:567761/150‑1 (MQ=255)
                                                                                                                                    tCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATa                  <  1:1503207/150‑1 (MQ=255)
                                                                                                                                      aTGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACg                >  2:4026927/1‑150 (MQ=255)
                                                                                                                                       tGGTAGCGAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACgg               >  2:4185119/1‑150 (MQ=255)
                                                                                                                                          tAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAg            >  2:137896/1‑150 (MQ=255)
                                                                                                                                           aGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAgg           <  2:4598830/150‑1 (MQ=255)
                                                                                                                                              gAATAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTATCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGaa        <  1:2469179/150‑1 (MQ=255)
                                                                                                                                              gAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGaa        >  1:3566042/1‑150 (MQ=255)
                                                                                                                                               aaTAACTCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGaaa       <  2:2242182/150‑1 (MQ=255)
                                                                                                                                                aTAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAAt      >  1:2762364/1‑150 (MQ=255)
                                                                                                                                                 tAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAAtt     >  1:3793071/1‑150 (MQ=255)
                                                                                                                                                  aaCGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAAttt    >  2:1138116/1‑150 (MQ=255)
                                                                                                                                                   aCGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAAtttt   >  2:3416126/1‑150 (MQ=255)
                                                                                                                                                    cGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTa  >  1:4128153/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTA  >  CP009273/212135‑212432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A