Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 290,073 G→T 29.4% F146L (TTC→TTA)  yagM ← CP4‑6 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273290,0730GT29.4% 532.0 / 193.3 254F146L (TTC→TTA) yagMCP4‑6 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (104/75);  new base T (36/39);  total (140/114)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 255 total aligned reads displayed.

ATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATGCTTTCTTTTATAGAGA  >  CP009273/289924‑290221
                                                                                                                                                     |                                                                                                                                                    
 taagaataaTGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGa                                                                                                                                                     >  2:1700174/1‑150 (MQ=255)
   agaataaTGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAAt                                                                                                                                                   <  2:791732/150‑1 (MQ=255)
     aataatGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATtc                                                                                                                                                 <  1:4248921/150‑1 (MQ=255)
     aataatGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATtc                                                                                                                                                 <  1:1407403/150‑1 (MQ=255)
         atGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATtcatca                                                                                                                                             >  1:601161/1‑150 (MQ=255)
           gTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCAtt                                                                                                                                           <  1:1522778/150‑1 (MQ=255)
           gTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCAtt                                                                                                                                           <  2:3727264/150‑1 (MQ=255)
               gAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCg                                                                                                                                       >  1:2934762/1‑150 (MQ=255)
                 atatCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAACTGTAAATGGCTTTTTCCATATCGTAATTTTCCCTGAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGtt                                                                                                                                     <  1:2451694/150‑1 (MQ=255)
                  tatCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTc                                                                                                                                    >  2:3826357/1‑150 (MQ=255)
                   atCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCa                                                                                                                                   <  2:500372/150‑1 (MQ=255)
                     ccTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGt                                                                                                                                 >  1:3364429/1‑150 (MQ=255)
                        gagaCACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGa                                                                                                                              >  2:2097907/1‑150 (MQ=255)
                        gagaCACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGa                                                                                                                              >  1:3813089/1‑150 (MQ=255)
                        gagaCACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGa                                                                                                                              >  2:2097925/1‑150 (MQ=255)
                            cacTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGt                                                                                                                          <  1:2177503/150‑1 (MQ=255)
                            cacTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGt                                                                                                                          >  2:2419503/1‑150 (MQ=255)
                              cTTTGATGCTTTACATCAAAATGTATTTGATTGTAAATGTAAATGGCTTTTTCCATATAGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTACTTCATCATTAGCGTTCAGTTGAAAGTaa                                                                                                                        <  1:1800762/150‑1 (MQ=255)
                                ttGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATc                                                                                                                      <  1:4522900/150‑1 (MQ=255)
                                ttGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATc                                                                                                                      <  1:3206597/150‑1 (MQ=255)
                                   aTGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGc                                                                                                                   <  2:3112014/150‑1 (MQ=255)
                                    tGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATTGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCa                                                                                                                  >  1:1592550/1‑150 (MQ=255)
                                    tGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCa                                                                                                                  <  1:1302193/150‑1 (MQ=255)
                                      cTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAAt                                                                                                                <  1:1026587/150‑1 (MQ=255)
                                       tttACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATg                                                                                                               >  1:4127292/1‑150 (MQ=255)
                                          aCATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATgggg                                                                                                            >  2:4358270/1‑150 (MQ=255)
                                          aCATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATgggg                                                                                                            <  2:338262/150‑1 (MQ=255)
                                            aTCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGAt                                                                                                          >  2:333376/1‑150 (MQ=255)
                                              cAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGAttt                                                                                                        >  1:4545110/1‑150 (MQ=255)
                                              cAAAATGTCTTTGATTGTAAATGTAAATGGC‑TTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAAGGGGGAtttt                                                                                                       >  2:3128689/1‑150 (MQ=255)
                                               aaaaTGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGAtttt                                                                                                       >  1:2810786/1‑150 (MQ=255)
                                                     tCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATgg                                                                                                 <  1:2213822/150‑1 (MQ=255)
                                                         tGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTc                                                                                             >  2:2680451/1‑150 (MQ=255)
                                                             tgtaaatgtaaaTGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCAACTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCatga                                                                                         <  1:4372961/150‑1 (MQ=255)
                                                              gtaaatgtaaatGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCatgat                                                                                        >  1:2159344/1‑150 (MQ=255)
                                                              gtaaatgtaaatGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCatgat                                                                                        >  1:2272484/1‑150 (MQ=255)
                                                              gtaaatgtaaatGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCatgat                                                                                        <  2:2002520/150‑1 (MQ=255)
                                                                aaatgtaaatgGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGt                                                                                      >  1:3827363/1‑150 (MQ=255)
                                                                 aatgtaaatgGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGtt                                                                                     >  1:2345330/1‑150 (MQ=255)
                                                                 aatgtaaatgGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGtt                                                                                     >  2:2244312/1‑150 (MQ=255)
                                                                   tgtaaatgGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCg                                                                                   >  1:1488447/1‑150 (MQ=255)
                                                                    gtaaatgGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGt                                                                                  <  1:865489/150‑1 (MQ=255)
                                                                    gtaaatgGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGt                                                                                  <  2:3309635/150‑1 (MQ=255)
                                                                       aatgGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGtttt                                                                               >  1:3624659/1‑150 (MQ=255)
                                                                          ggCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCa                                                                            >  1:3958523/1‑150 (MQ=255)
                                                                          ggCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCa                                                                            >  1:895876/1‑150 (MQ=255)
                                                                           gCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAg                                                                           >  2:2768623/1‑150 (MQ=255)
                                                                                 tCCATATCGTAATTTTCCCTTAGCGAGGATAGACTTTCACGTATAACTTTTTTTTCATCTTTCCCCATGAATTCATCATTATCGTTAAGTTGAAAGTAAAATGCAATTGGGATATTTAATAGACTCATGATGTTCGTTTTCAAGATTAAc                                                                     >  1:977949/1‑150 (MQ=255)
                                                                                  ccATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACg                                                                    <  2:4238629/150‑1 (MQ=255)
                                                                                  ccATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACg                                                                    >  1:2079658/1‑150 (MQ=255)
                                                                                  ccATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACg                                                                    >  1:4410039/1‑150 (MQ=255)
                                                                                    atatCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGaa                                                                  >  1:4201422/1‑150 (MQ=255)
                                                                                      atCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACa                                                                >  1:2586252/1‑150 (MQ=255)
                                                                                      atCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTT‑ATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAg                                                               >  2:3866516/1‑150 (MQ=255)
                                                                                        cGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGt                                                              <  2:4548664/150‑1 (MQ=255)
                                                                                          tAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTAc                                                            <  2:1567025/150‑1 (MQ=255)
                                                                                           aaTTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTAct                                                           <  2:32522/150‑1 (MQ=255)
                                                                                           aaTTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTAct                                                           >  2:3944251/1‑150 (MQ=255)
                                                                                             ttttCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTActct                                                         >  1:3994938/1‑150 (MQ=255)
                                                                                              tttCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCtt                                                        <  2:1975950/150‑1 (MQ=255)
                                                                                              tttCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCtt                                                        <  2:2060257/150‑1 (MQ=255)
                                                                                               ttCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTc                                                       >  1:2632362/1‑150 (MQ=255)
                                                                                                   cTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCaga                                                   >  2:1070351/1‑150 (MQ=255)
                                                                                                           ggATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTc                                           >  2:837799/1‑150 (MQ=255)
                                                                                                             aTAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTg                                         >  1:3523865/1‑150 (MQ=255)
                                                                                                             aTAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTACTg                                         >  1:3523772/1‑150 (MQ=255)
                                                                                                              tAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGt                                        >  2:835366/1‑150 (MQ=255)
                                                                                                              tAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGt                                        <  1:2452057/150‑1 (MQ=255)
                                                                                                               aGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGtt                                       <  2:4131910/150‑1 (MQ=255)
                                                                                                               aGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGtt                                       >  2:2882801/1‑150 (MQ=255)
                                                                                                                gCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGttt                                      >  2:1857803/1‑150 (MQ=255)
                                                                                                                gCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGttt                                      <  2:3637544/150‑1 (MQ=255)
                                                                                                                 ccTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGtttt                                     <  1:1139828/150‑1 (MQ=255)
                                                                                                                 ccTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGtttt                                     <  2:2946744/150‑1 (MQ=255)
                                                                                                                  cTTTCACGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTa                                    >  1:2584412/1‑150 (MQ=255)
                                                                                                                     tCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCt                                 >  1:1841463/1‑150 (MQ=255)
                                                                                                                       aCGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCg                               <  1:332900/150‑1 (MQ=255)
                                                                                                                       aCGTATAACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCg                               <  1:1647227/150‑1 (MQ=255)
                                                                                                                           ataACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCtt                           >  2:326627/1‑150 (MQ=255)
                                                                                                                            taACTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTc                          <  2:2345330/150‑1 (MQ=255)
                                                                                                                            taACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTc                          <  2:164966/150‑1 (MQ=255)
                                                                                                                             aaCTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCg                         >  2:1768987/1‑150 (MQ=255)
                                                                                                                             aaCTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCg                         <  2:4628789/150‑1 (MQ=255)
                                                                                                                               cTGTTTTTTCATCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGAt                       >  1:809754/1‑150 (MQ=255)
                                                                                                                                  tattttCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGa                    <  2:1084445/148‑1 (MQ=255)
                                                                                                                                   tttttCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGAt                   >  1:82540/1‑150 (MQ=255)
                                                                                                                                   tttttCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGAt                   >  1:938942/1‑150 (MQ=255)
                                                                                                                                   tttttCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGAt                   <  2:4460523/150‑1 (MQ=255)
                                                                                                                                    ttttCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATg                  <  2:610548/150‑1 (MQ=255)
                                                                                                                                     tttCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTTAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGAttc                 <  2:756036/150‑3 (MQ=255)
                                                                                                                                     tttCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATGc                 <  2:756090/150‑1 (MQ=255)
                                                                                                                                     tttCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATGc                 >  2:642752/1‑150 (MQ=255)
                                                                                                                                       tCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATGctt               <  1:3145829/150‑1 (MQ=255)
                                                                                                                                        cATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATGcttt              <  2:1419314/150‑1 (MQ=255)
                                                                                                                                        cATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATGcttt              >  1:2362241/1‑150 (MQ=255)
                                                                                                                                         aTCTTTCCCCATTAATTCATCATTAGCGTTCAGTTGAAAGTAATCCGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATGctttc             <  1:759518/150‑1 (MQ=255)
                                                                                                                                         aTCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATGctttc             >  1:2331578/1‑150 (MQ=255)
                                                                                                                                             ttCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATGCTTTCtttt         <  1:2680451/150‑1 (MQ=255)
                                                                                                                                             ttCCCAATTTATTCATCTTTAGCGTTGTGTTGAAAGTAATCTGCAATGTTGATTTTTAATGGTCTCATGATGTTCGTTTTCCAGATTAACGTACATTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATGCTTTCtttt         <  2:3624290/150‑1 (MQ=255)
                                                                                                                                                 ccATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATGCTTTCTTTTATag     <  1:4480906/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTATAACTGTTTTTTCATCTTTCCCCATGAATTCATCATTAGCGTTCAGTTGAAAGTAATCTGCAATGGGGATTTTTAATGGACTCATGATGTTCGTTTTCCAGATTAACGAACAGTACTCTTCCAGAGTTACTTCTGTTTTACCTCGGCTTCGATTGATGCTTTCTTTTATAGAGA  >  CP009273/289924‑290221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A