Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 834,612 G→T 29.3% R18S (CGC→AGC)  ybiX ← Fe(II)‑dependent oxygenase superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273834,6120GT29.3% 636.7 / 229.1 296R18S (CGC→AGC) ybiXFe(II)‑dependent oxygenase superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (76/133);  new base T (30/57);  total (106/190)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 305 total aligned reads displayed.

ATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACG  >  CP009273/834463‑834761
                                                                                                                                                     |                                                                                                                                                     
     ggTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAAc                                                                                                                                                  <  1:1167112/150‑1 (MQ=255)
     ggTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAAc                                                                                                                                                  >  2:237751/1‑150 (MQ=255)
     ggTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAAc                                                                                                                                                  <  2:4421517/150‑1 (MQ=255)
      gTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACg                                                                                                                                                 <  2:994143/150‑1 (MQ=255)
      gTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACg                                                                                                                                                 <  2:1915092/150‑1 (MQ=255)
      gTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACg                                                                                                                                                 <  2:3317346/150‑1 (MQ=255)
       ttAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGa                                                                                                                                                >  2:3081658/1‑150 (MQ=255)
        tAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAg                                                                                                                                               <  1:3871948/150‑1 (MQ=255)
        tAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAg                                                                                                                                               <  1:4254408/150‑1 (MQ=255)
         aaCCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGc                                                                                                                                              <  2:4236078/150‑1 (MQ=255)
         aaCCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGc                                                                                                                                              >  1:3233384/1‑150 (MQ=255)
         aaCCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGc                                                                                                                                              <  2:1905449/150‑1 (MQ=255)
           ccGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCGTTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGa                                                                                                                                            <  1:1916923/150‑1 (MQ=255)
           ccGAGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGa                                                                                                                                            <  1:4131372/150‑1 (MQ=255)
             gcgTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACg                                                                                                                                          <  2:4440728/150‑1 (MQ=255)
              cgTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGt                                                                                                                                         >  2:4575028/1‑150 (MQ=255)
              cgTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGt                                                                                                                                         >  1:2315903/1‑150 (MQ=255)
                 tCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCt                                                                                                                                      >  1:208027/1‑150 (MQ=255)
                 tCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCt                                                                                                                                      <  2:2027170/150‑1 (MQ=255)
                   aGCACCTCATTTTACAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCtgt                                                                                                                                    >  2:3592714/1‑150 (MQ=255)
                      aCCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGc                                                                                                                                 <  1:3961213/150‑1 (MQ=255)
                      aCCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGc                                                                                                                                 <  1:1828016/150‑1 (MQ=255)
                      aCCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGc                                                                                                                                 <  2:1244927/150‑1 (MQ=255)
                       ccTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCg                                                                                                                                >  2:3138250/1‑150 (MQ=255)
                         tCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGAt                                                                                                                              <  2:1748664/150‑1 (MQ=255)
                          cATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATa                                                                                                                             >  2:4389091/1‑150 (MQ=255)
                           aTTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATaa                                                                                                                            >  2:2627858/1‑150 (MQ=255)
                                      cggcgTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGaa                                                                                                                 <  2:640857/150‑1 (MQ=255)
                                      aggcgTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTGCTTAACATGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACTTCCTGTGGCGATAACACGCCGGGaa                                                                                                                 >  2:3637789/2‑150 (MQ=255)
                                       ggcgTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAAt                                                                                                                <  2:2557014/150‑1 (MQ=255)
                                        gcgTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAAtg                                                                                                               <  1:3035123/150‑1 (MQ=255)
                                         cgTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAAtgt                                                                                                              <  2:3990234/150‑1 (MQ=255)
                                          gTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAAtgtg                                                                                                             <  2:945746/150‑1 (MQ=255)
                                          gTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAAtgtg                                                                                                             <  2:2093327/150‑1 (MQ=255)
                                          gTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAAtgtg                                                                                                             >  2:4489889/1‑150 (MQ=255)
                                           tataACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTgg                                                                                                            <  2:2921/150‑1 (MQ=255)
                                              aaCGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTAc                                                                                                         <  1:594069/150‑1 (MQ=255)
                                                cGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTAcat                                                                                                       <  1:3541439/150‑1 (MQ=255)
                                                 gTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTAcatc                                                                                                      <  2:524895/150‑1 (MQ=255)
                                                  tgctgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTAcatca                                                                                                     >  1:304912/1‑150 (MQ=255)
                                                  tgctgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTAcatca                                                                                                     <  1:1506187/150‑1 (MQ=255)
                                                    ctgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATa                                                                                                   <  1:4534532/150‑1 (MQ=255)
                                                     tgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATAc                                                                                                  <  1:1386067/150‑1 (MQ=255)
                                                            gtCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTtct                                                                                           >  2:1478500/1‑150 (MQ=255)
                                                               gACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCa                                                                                        <  1:3198071/150‑1 (MQ=255)
                                                               gACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCa                                                                                        <  1:1774841/150‑1 (MQ=255)
                                                               gACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCa                                                                                        >  1:1545147/1‑150 (MQ=255)
                                                                 ccTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCaaa                                                                                      >  2:602165/1‑150 (MQ=255)
                                                                    gTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAg                                                                                   <  2:2999472/150‑1 (MQ=255)
                                                                    gTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAg                                                                                   <  1:730083/150‑1 (MQ=255)
                                                                    gTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAg                                                                                   >  2:1672971/1‑150 (MQ=255)
                                                                     ttGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGt                                                                                  <  2:3402648/150‑1 (MQ=255)
                                                                          tGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGc                                                                             <  1:4208298/150‑1 (MQ=255)
                                                                            ttCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCt                                                                           <  1:4644213/150‑1 (MQ=255)
                                                                            ttCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTAGGCTTGTTCCAGTTGTTCTCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCt                                                                           <  1:2125251/150‑1 (MQ=255)
                                                                              cTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTgc                                                                         >  1:4320996/1‑150 (MQ=255)
                                                                              cTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTgc                                                                         <  1:3458755/150‑1 (MQ=255)
                                                                               ttAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTgcg                                                                        >  2:2593236/1‑150 (MQ=255)
                                                                                 aaCTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGcc                                                                      <  2:1579509/150‑1 (MQ=255)
                                                                                  aCTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGccc                                                                     >  1:906750/1‑150 (MQ=255)
                                                                                   cTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGcccc                                                                    <  2:2862206/150‑1 (MQ=255)
                                                                                    ttGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCca                                                                   <  1:1047233/150‑1 (MQ=255)
                                                                                     tGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCcac                                                                  <  2:409109/150‑1 (MQ=255)
                                                                                      gcgcACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCcaca                                                                 <  2:2145716/150‑1 (MQ=255)
                                                                                          aCCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTg                                                             >  1:1015198/1‑150 (MQ=255)
                                                                                           ccTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGa                                                            <  2:74304/150‑1 (MQ=255)
                                                                                           ccTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGa                                                            <  2:4113114/150‑1 (MQ=255)
                                                                                             ttGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAAt                                                          <  2:2416765/150‑1 (MQ=255)
                                                                                              tgtgCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATc                                                         <  2:3079588/150‑1 (MQ=255)
                                                                                               gtgCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCa                                                        >  2:1028932/1‑150 (MQ=255)
                                                                                                tgCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGTATCAg                                                       >  2:1636395/1‑150 (MQ=255)
                                                                                                 gcgcCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGa                                                      >  1:3749583/1‑150 (MQ=255)
                                                                                                 gcgcCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGa                                                      >  1:3692479/1‑150 (MQ=255)
                                                                                                  cgcCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGaa                                                     <  1:4089387/150‑1 (MQ=255)
                                                                                                    ccGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAAt                                                   <  2:2894050/150‑1 (MQ=255)
                                                                                                    ccGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAAt                                                   <  1:3346862/150‑1 (MQ=255)
                                                                                                      ggtggtGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGc                                                 >  2:3182426/1‑150 (MQ=255)
                                                                                                         ggtgACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCata                                              <  2:1582104/150‑1 (MQ=255)
                                                                                                           tgACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATAtt                                            <  2:1410994/150‑1 (MQ=255)
                                                                                                                cgTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCtg                                       <  1:2570621/150‑1 (MQ=255)
                                                                                                                 gTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCtgt                                      <  2:1963717/150‑1 (MQ=255)
                                                                                                                  tccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCtgtg                                     <  1:523096/150‑1 (MQ=255)
                                                                                                                   ccatccaCCCATTCGGCTTTTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGAGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGa                                    >  2:1520687/1‑150 (MQ=255)
                                                                                                                   ccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGa                                    >  1:1672757/1‑150 (MQ=255)
                                                                                                                    catccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAg                                   >  1:1221945/1‑150 (MQ=255)
                                                                                                                     atccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGc                                  <  2:370280/150‑1 (MQ=255)
                                                                                                                      tccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCa                                 <  1:495334/150‑1 (MQ=255)
                                                                                                                            cATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAgg                           >  2:3903194/1‑150 (MQ=255)
                                                                                                                               tCGGCTTGTTCCAGTTGTTCGCTAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTc                        <  1:2848/150‑1 (MQ=255)
                                                                                                                                cGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCt                       >  1:3996007/1‑150 (MQ=255)
                                                                                                                                cGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCt                       <  1:4451194/150‑1 (MQ=255)
                                                                                                                                 ggCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAGTCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCtt                      <  1:1268747/150‑1 (MQ=255)
                                                                                                                                 ggCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCtt                      <  2:2230764/150‑1 (MQ=255)
                                                                                                                                   cTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTgg                    >  1:4623763/1‑150 (MQ=255)
                                                                                                                                     tGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCt                  <  1:2665934/150‑1 (MQ=255)
                                                                                                                                             ttgttCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCggg          <  2:891045/150‑1 (MQ=255)
                                                                                                                                                 tCGCGAAACCGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGAt      <  2:4400502/150‑1 (MQ=255)
                                                                                                                                                 tCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGAt      <  1:3081658/150‑1 (MQ=255)
                                                                                                                                                   gcgAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATaa    >  2:720918/1‑150 (MQ=255)
                                                                                                                                                     gAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACg  <  1:1655621/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACG  >  CP009273/834463‑834761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A