Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 896,095 C→A 39.0% intergenic (‑64/+227) artJ ← / ← artM arginine binding protein, periplasmic/arginine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273896,0950CA39.0% 350.2 / 276.2 259intergenic (‑64/+227)artJ/artMarginine binding protein, periplasmic/arginine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (77/81);  new base A (32/69);  total (109/150)
Fisher's exact test for biased strand distribution p-value = 6.99e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

CTGAAACGCCAAAATTGATTTTCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACA  >  CP009273/895947‑896241
                                                                                                                                                    |                                                                                                                                                  
cTGAAACGCCAAAATTGATTTTCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGcc                                                                                                                                                   <  2:2158703/150‑1 (MQ=255)
  gAAACGCCAAAATTGATTTTCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACAt                                                                                                                                                 >  1:3032645/1‑150 (MQ=255)
   aaaCGCCAAAATTGATTTTCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATc                                                                                                                                                <  1:1520382/150‑1 (MQ=255)
     aCGCCAAAATTGATTTTCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCaa                                                                                                                                              <  1:1777924/150‑1 (MQ=255)
        ccAAAATTGATTTTCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTg                                                                                                                                           <  1:3571063/150‑1 (MQ=255)
          aaaaTTGATTTTCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGaa                                                                                                                                         >  2:3692285/1‑150 (MQ=255)
          aaaaTTGATTTTCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGaa                                                                                                                                         >  2:4474820/1‑150 (MQ=255)
            aaTTGATTTTCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAAtt                                                                                                                                       <  1:1953173/150‑1 (MQ=255)
             aTTGATTTTCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAAttt                                                                                                                                      <  2:3818075/150‑1 (MQ=255)
             aTTGATTTTCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAAttt                                                                                                                                      <  1:1312149/150‑1 (MQ=255)
               tGATTTTCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTtat                                                                                                                                    >  1:3846708/1‑150 (MQ=255)
                 aTTTTCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTatat                                                                                                                                  <  2:1894847/150‑1 (MQ=255)
                   tttCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGc                                                                                                                                <  2:1685358/150‑1 (MQ=255)
                      ctctGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAAt                                                                                                                             >  1:1496288/1‑150 (MQ=255)
                         tGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATaaa                                                                                                                          <  2:3428049/150‑1 (MQ=255)
                         tGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATaaa                                                                                                                          <  2:1867227/150‑1 (MQ=255)
                           cGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACa                                                                                                                        <  1:514723/150‑1 (MQ=255)
                             gCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATg                                                                                                                      <  1:4332154/150‑1 (MQ=255)
                                 aaGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTa                                                                                                                  <  1:473089/150‑1 (MQ=255)
                                 aaGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTa                                                                                                                  >  1:3745511/1‑150 (MQ=255)
                                 aaGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTa                                                                                                                  >  1:2949106/1‑150 (MQ=255)
                                   gCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTaaa                                                                                                                <  1:2448706/150‑1 (MQ=255)
                                   gCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTaaa                                                                                                                <  1:3993380/150‑1 (MQ=255)
                                     aCCGAAAGTAAAGGAAGCAAGTAAAGCGTCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAaata                                                                                                              <  2:4367105/150‑1 (MQ=255)
                                          aaGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTa                                                                                                         >  2:4529650/1‑150 (MQ=255)
                                           aGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTaa                                                                                                        >  1:3332487/1‑150 (MQ=255)
                                           aGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTaa                                                                                                        >  1:2027543/1‑150 (MQ=255)
                                             tAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAAt                                                                                                      <  2:361662/150‑1 (MQ=255)
                                             tAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAAt                                                                                                      >  2:2667547/1‑150 (MQ=255)
                                              aaaGGAAGCAAGTACAGCGGCAAGAACTACCTTTTTCATGCTGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTTCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATg                                                                                                     <  2:3518858/150‑1 (MQ=255)
                                              aaaGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATg                                                                                                     <  2:1658668/150‑1 (MQ=255)
                                               aaGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGa                                                                                                    >  2:326259/1‑150 (MQ=255)
                                                   aaGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATa                                                                                                >  1:2195515/1‑150 (MQ=255)
                                                    aGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATaa                                                                                               >  2:4098406/1‑150 (MQ=255)
                                                         gTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAg                                                                                          <  2:4599897/150‑1 (MQ=255)
                                                           aaaGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGAc                                                                                        >  2:531099/1‑150 (MQ=255)
                                                           aaaGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGAc                                                                                        <  2:3645885/150‑1 (MQ=255)
                                                            aaGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACg                                                                                       <  1:3929004/150‑1 (MQ=255)
                                                                 gCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACaa                                                                                  <  2:80712/150‑1 (MQ=255)
                                                                 gCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACaa                                                                                  <  1:2468455/150‑1 (MQ=255)
                                                                 gCAAGAACTAACTTTTTCATGAGGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACaa                                                                                  <  2:4462981/150‑1 (MQ=255)
                                                                  cAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAAc                                                                                 <  2:1689462/150‑1 (MQ=255)
                                                                   aaGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACt                                                                                <  1:3433642/150‑1 (MQ=255)
                                                                   aaGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACt                                                                                <  1:1665368/150‑1 (MQ=255)
                                                                      aactaactTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAg                                                                             >  2:596910/1‑150 (MQ=255)
                                                                        ctaactTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTg                                                                           <  2:4223284/150‑1 (MQ=255)
                                                                         taactTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTgg                                                                          <  2:2025052/150‑1 (MQ=255)
                                                                         taactTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTgg                                                                          >  2:1519947/1‑150 (MQ=255)
                                                                          aactTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTggg                                                                         >  1:1415048/1‑150 (MQ=255)
                                                                            ctTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGtt                                                                       <  1:2470216/150‑1 (MQ=255)
                                                                            ctTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGtt                                                                       <  1:1326217/150‑1 (MQ=255)
                                                                            ctTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGtt                                                                       <  2:4263149/150‑1 (MQ=255)
                                                                             tttttCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTg                                                                      <  2:433260/150‑1 (MQ=255)
                                                                                 tCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCg                                                                  >  2:2186484/1‑150 (MQ=255)
                                                                                  cATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGt                                                                 <  2:3770833/150‑1 (MQ=255)
                                                                                  cATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGt                                                                 <  2:1271107/150‑1 (MQ=255)
                                                                                   atgatgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTc                                                                >  2:2413536/1‑150 (MQ=255)
                                                                                     gatgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCtt                                                              <  1:3140415/150‑1 (MQ=255)
                                                                                      atgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTc                                                             <  1:3166559/150‑1 (MQ=255)
                                                                                      atgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTc                                                             >  2:2780558/1‑150 (MQ=255)
                                                                                      atgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTc                                                             >  2:28808/1‑150 (MQ=255)
                                                                                        ggAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCAt                                                           <  1:2831436/150‑1 (MQ=255)
                                                                                        ggAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCAt                                                           <  2:722585/150‑1 (MQ=255)
                                                                                        ggAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCAt                                                           <  2:514696/150‑1 (MQ=255)
                                                                                         gAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCAtt                                                          >  1:2772632/1‑150 (MQ=255)
                                                                                          aaCTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCAttg                                                         <  1:3610539/150‑2 (MQ=255)
                                                                                              cccGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAg                                                     <  1:4528201/150‑1 (MQ=255)
                                                                                                   cTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAAttt                                                >  1:2096145/1‑150 (MQ=255)
                                                                                                   cTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAAttt                                                >  1:3900216/1‑150 (MQ=255)
                                                                                                   cTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAAttt                                                >  2:3170914/1‑150 (MQ=255)
                                                                                                   cTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAAttt                                                >  2:3171409/1‑150 (MQ=255)
                                                                                                   cTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCAGTATAAGAAttt                                                >  1:259342/1‑150 (MQ=255)
                                                                                                     gTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTAt                                              <  1:4363537/150‑1 (MQ=255)
                                                                                                     gTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTAt                                              <  1:4578499/150‑1 (MQ=255)
                                                                                                     gTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTAt                                              <  1:4363607/150‑1 (MQ=255)
                                                                                                           cTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTa                                        <  2:4381147/150‑1 (MQ=255)
                                                                                                           cTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTa                                        >  2:959062/1‑150 (MQ=255)
                                                                                                            ttATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTAt                                       >  1:4227704/1‑150 (MQ=255)
                                                                                                              aTGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGt                                     <  2:4323798/150‑1 (MQ=255)
                                                                                                                gATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAg                                   <  2:3728823/150‑1 (MQ=255)
                                                                                                                  tttttGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGc                                 <  1:1108718/150‑1 (MQ=255)
                                                                                                                  tttttGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGc                                 >  2:567657/1‑150 (MQ=255)
                                                                                                                    tttGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCg                               <  1:3100398/150‑1 (MQ=255)
                                                                                                                     ttGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCgg                              <  2:163350/150‑1 (MQ=255)
                                                                                                                     ttGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCgg                              <  2:161724/150‑1 (MQ=255)
                                                                                                                         ccGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATaa                          >  1:2132028/1‑150 (MQ=255)
                                                                                                                          cGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAg                         >  2:2301778/1‑150 (MQ=255)
                                                                                                                           gtgtCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACCTGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAgg                        <  2:3348393/150‑1 (MQ=255)
                                                                                                                             gtCTGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCg                      >  1:230410/1‑150 (MQ=255)
                                                                                                                                tGCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTcc                   <  1:987177/150‑1 (MQ=255)
                                                                                                                                 gCGGCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTccc                  <  2:2970625/150‑1 (MQ=255)
                                                                                                                                    gCATGGGATAACAATGACATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCccgc               <  1:3389254/150‑1 (MQ=255)
                                                                                                                                     cATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCccgcc              >  1:4294552/1‑150 (MQ=255)
                                                                                                                                      aTGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCccgccg             >  1:833411/1‑150 (MQ=255)
                                                                                                                                        gggATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCa           >  2:2215126/1‑150 (MQ=255)
                                                                                                                                         ggATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCAt          >  1:1085325/1‑150 (MQ=255)
                                                                                                                                          gATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATc         >  2:1079378/1‑150 (MQ=255)
                                                                                                                                           aTAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATcc        <  1:570938/150‑1 (MQ=255)
                                                                                                                                           aTAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATcc        >  1:1630854/1‑150 (MQ=255)
                                                                                                                                                 aTGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGcaca  >  1:2624931/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CTGAAACGCCAAAATTGATTTTCTCTGCGGCAGAAGCACCGAAAGTAAAGGAAGCAAGTAAAGCGGCAAGAACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACA  >  CP009273/895947‑896241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A