Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 912,614 G→T 14.1% R229L (CGA→CTA)  ybjD → putative OLD family ATP‑dependent endonuclease; DUF2813 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273912,6140GT14.1% 865.4 / 79.9 255R229L (CGA→CTA) ybjDputative OLD family ATP‑dependent endonuclease; DUF2813 family protein
Reads supporting (aligned to +/- strand):  ref base G (109/110);  new base T (20/16);  total (129/126)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 259 total aligned reads displayed.

GGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACTTTGTTGCAGGCAAAAG  >  CP009273/912465‑912760
                                                                                                                                                     |                                                                                                                                                   
    gTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTatat                                                                                                                                                 >  1:1278773/1‑150 (MQ=255)
    gTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGatat                                                                                                                                                 >  2:863668/1‑150 (MQ=255)
    gTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGatat                                                                                                                                                 >  1:4497883/1‑150 (MQ=255)
       aCCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGt                                                                                                                                              >  2:2847246/1‑150 (MQ=255)
         cgcgcgCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGttt                                                                                                                                            <  1:82342/150‑1 (MQ=255)
            gcgcCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAAt                                                                                                                                         >  2:4634934/1‑150 (MQ=255)
                cAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATgcgg                                                                                                                                     >  2:3077192/1‑150 (MQ=255)
                  gCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGTGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATgcggcg                                                                                                                                   <  1:475058/150‑1 (MQ=255)
                  gCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATgcggcg                                                                                                                                   <  1:2692012/150‑1 (MQ=255)
                   cTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATgcggcgg                                                                                                                                  <  1:1432637/150‑1 (MQ=255)
                   cTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATgcggcgg                                                                                                                                  <  1:996283/150‑1 (MQ=255)
                      gATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGa                                                                                                                               >  1:2302154/1‑150 (MQ=255)
                               gCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AAt                                                                                                                     >  1:3609193/1‑150 (MQ=255)
                               gCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AAt                                                                                                                     >  1:3487585/1‑150 (MQ=255)
                               gCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AAt                                                                                                                     <  2:2247116/150‑1 (MQ=255)
                                cccGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATg                                                                                                                    <  2:2517160/150‑1 (MQ=255)
                                 ccGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGa                                                                                                                   <  2:4437195/150‑1 (MQ=255)
                                  cGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAg                                                                                                                  <  1:2975820/150‑1 (MQ=255)
                                   gTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACCGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGc                                                                                                                 >  2:3723707/1‑150 (MQ=255)
                                    tGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCa                                                                                                                >  2:3012087/1‑150 (MQ=255)
                                     gAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCaa                                                                                                               <  1:705434/150‑1 (MQ=255)
                                     gAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCaa                                                                                                               <  2:1862875/150‑1 (MQ=255)
                                      aGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCACTGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAAc                                                                                                              >  2:4228861/1‑150 (MQ=255)
                                         tATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGaa                                                                                                           <  2:658667/150‑1 (MQ=255)
                                               cacaTCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGgc                                                                                                     >  1:1181565/1‑150 (MQ=255)
                                               cacaTCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGgc                                                                                                     <  2:3103834/150‑1 (MQ=255)
                                               cacaTCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGgc                                                                                                     >  1:2148910/1‑150 (MQ=255)
                                                  aTCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCt                                                                                                  <  1:538458/150‑1 (MQ=255)
                                                  aTCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCt                                                                                                  <  2:3861247/150‑1 (MQ=255)
                                                      gCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCt                                                                                              <  2:352302/150‑1 (MQ=255)
                                                      gCAAAATCTCTCTGAGGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCt                                                                                              <  2:1129701/150‑1 (MQ=255)
                                                          aaTCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGat                                                                                          >  1:2540217/1‑150 (MQ=255)
                                                          aaTCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGat                                                                                          >  1:3548024/1‑150 (MQ=255)
                                                           aTCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGata                                                                                         >  2:3156440/1‑150 (MQ=255)
                                                               ctctGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATatcat                                                                                     >  2:1287791/1‑150 (MQ=255)
                                                                tctGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATatcatc                                                                                    <  1:4420759/150‑1 (MQ=255)
                                                                  tGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCaa                                                                                  <  1:3408478/150‑1 (MQ=255)
                                                                   gATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAAc                                                                                 >  1:2995566/1‑150 (MQ=255)
                                                                     tGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCg                                                                               >  2:1063172/1‑150 (MQ=255)
                                                                         cAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGgatg                                                                           <  2:3781987/150‑1 (MQ=255)
                                                                         cAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGgatg                                                                           <  2:2369595/150‑1 (MQ=255)
                                                                         cAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGgatg                                                                           <  2:577381/150‑1 (MQ=255)
                                                                          aGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGgatga                                                                          >  2:1911352/1‑150 (MQ=255)
                                                                          aGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGgatga                                                                          <  1:3824579/150‑1 (MQ=255)
                                                                            aTTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGAtt                                                                        <  1:4245100/150‑1 (MQ=255)
                                                                             ttCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTg                                                                       <  2:3623241/150‑1 (MQ=255)
                                                                              tCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTga                                                                      <  2:215712/150‑1 (MQ=255)
                                                                              tCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTga                                                                      <  2:2058637/150‑1 (MQ=255)
                                                                              tCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTga                                                                      >  2:3226328/1‑150 (MQ=255)
                                                                              tCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTga                                                                      >  1:941162/1‑150 (MQ=255)
                                                                                  cAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTgaccga                                                                  >  1:158961/1‑150 (MQ=255)
                                                                                  cAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTgaccga                                                                  <  1:2670947/150‑1 (MQ=255)
                                                                                   aGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTgaccgac                                                                 <  2:4413087/150‑1 (MQ=255)
                                                                                      gACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCtg                                                              >  2:3652429/1‑150 (MQ=255)
                                                                                        cTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCtggt                                                            <  2:2620015/150‑1 (MQ=255)
                                                                                        cTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCtggt                                                            <  1:409399/150‑1 (MQ=255)
                                                                                          ttCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCtggtgg                                                          >  2:2801525/1‑150 (MQ=255)
                                                                                           tCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTggg                                                         <  2:2442091/150‑1 (MQ=255)
                                                                                           tCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTggg                                                         >  1:768116/1‑150 (MQ=255)
                                                                                               cAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCt                                                     <  2:3318918/150‑1 (MQ=255)
                                                                                                aaTGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTc                                                    >  1:1001516/1‑150 (MQ=255)
                                                                                                  tGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGt                                                  <  1:2890286/150‑1 (MQ=255)
                                                                                                   ggTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTa                                                 >  1:3248007/1‑150 (MQ=255)
                                                                                                     tACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATc                                               <  2:4355625/150‑1 (MQ=255)
                                                                                                      aCAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCg                                              >  2:4490451/1‑150 (MQ=255)
                                                                                                       cAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCgg                                             >  1:564468/1‑150 (MQ=255)
                                                                                                       cAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCgg                                             >  1:3351342/1‑150 (MQ=255)
                                                                                                       cAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCgg                                             <  1:863668/150‑1 (MQ=255)
                                                                                                        aGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCggg                                            <  1:2259919/150‑1 (MQ=255)
                                                                                                        aGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCggg                                            >  1:170458/1‑150 (MQ=255)
                                                                                                             ctTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTAtt                                       >  1:4034375/1‑150 (MQ=255)
                                                                                                             ctTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTAtt                                       <  1:1769439/150‑1 (MQ=255)
                                                                                                              ttGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTAttt                                      >  2:727707/1‑150 (MQ=255)
                                                                                                              ttGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTAttt                                      >  1:1233479/1‑150 (MQ=255)
                                                                                                                 aGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTCTCGGGTTATTTTGc                                   >  2:1239892/1‑150 (MQ=255)
                                                                                                                 aGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGc                                   >  2:4159972/1‑150 (MQ=255)
                                                                                                                   cATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTc                                 >  1:1176201/1‑150 (MQ=255)
                                                                                                                    attattTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAAAGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCg                                >  2:4536058/1‑150 (MQ=255)
                                                                                                                         ttCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTa                           <  2:3338817/150‑1 (MQ=255)
                                                                                                                           ctctGAGCAGGGGGCCGGACAGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTAtt                         >  1:2468384/1‑150 (MQ=255)
                                                                                                                           ctctGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTAtt                         >  1:3263653/1‑150 (MQ=255)
                                                                                                                             ctGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTg                       <  2:3735423/150‑1 (MQ=255)
                                                                                                                               gAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCt                     <  2:3465123/150‑1 (MQ=255)
                                                                                                                                 gCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTAc                   <  2:208697/150‑1 (MQ=255)
                                                                                                                                   aGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACtt                 >  2:1776496/1‑150 (MQ=255)
                                                                                                                                   aGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACtt                 >  1:188043/1‑150 (MQ=255)
                                                                                                                                    gggggCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACttt                >  1:1025953/1‑150 (MQ=255)
                                                                                                                                    gggggCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACttt                <  2:3609930/150‑1 (MQ=255)
                                                                                                                                    gggggCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACttt                <  2:1706518/150‑1 (MQ=255)
                                                                                                                                     ggggCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACTttg               <  1:2789160/150‑1 (MQ=255)
                                                                                                                                     ggggCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACTttg               >  1:3297584/1‑150 (MQ=255)
                                                                                                                                      gggCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACTttgt              <  2:2574750/150‑1 (MQ=255)
                                                                                                                                         ccGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACTTTGTTGc           <  1:3875590/150‑1 (MQ=255)
                                                                                                                                           ggACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGCAAATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACTTTGTTGCa          <  2:1607776/150‑1 (MQ=255)
                                                                                                                                             aCAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGGTTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACTTTGTTGCAGGc       >  1:1619070/1‑150 (MQ=255)
                                                                                                                                             aCAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACTTTGTTGCAGGc       <  2:3262623/150‑1 (MQ=255)
                                                                                                                                               aGGCGCTATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACTTTGTTGCAGGCaa     >  2:4609166/1‑150 (MQ=255)
                                                                                                                                                ggCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACTTTGTTGCAGGCaaa    >  2:3487327/1‑150 (MQ=255)
                                                                                                                                                ggCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACTTTGTTGCAGGCaaa    >  2:3064653/1‑150 (MQ=255)
                                                                                                                                                  cgcgATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACTTTGTTGCAGGCAAAAg  <  2:2862152/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAATGGTACAGCTGCTTGAGCATTATTTCTCTGAGCAGGGGGCCGGACAGGCGCGATATCGTTTAATGCGGCGGCGAGCCAGC‑AATGAGCAACGAAGCTGGCGCTATCTGGATATCATCAACCGGATGATTGACCGACCTGGTGGGCGCTCGTATCGGGTTATTTTGCTCGGCCTATTTGCTACTTTGTTGCAGGCAAAAG  >  CP009273/912465‑912760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A