Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 918,410 G→T 31.1% A14A (GCG→GCT)  clpS → regulatory protein for ClpA substrate specificity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273918,4100GT31.1% 576.6 / 238.9 289A14A (GCG→GCT) clpSregulatory protein for ClpA substrate specificity
Reads supporting (aligned to +/- strand):  ref base G (80/119);  new base T (38/52);  total (118/171)
Fisher's exact test for biased strand distribution p-value = 7.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 291 total aligned reads displayed.

GATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAATTGATGCTCG  >  CP009273/918262‑918558
                                                                                                                                                    |                                                                                                                                                    
gATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATTATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCgg                                                                                                                                                     <  2:1056518/150‑1 (MQ=255)
 aTAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGga                                                                                                                                                    >  2:4307233/1‑150 (MQ=255)
   aGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGgaag                                                                                                                                                  >  1:3249203/1‑150 (MQ=255)
     atgatAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGCACGACTGGCTGGACTTTGATCAACTGGCGgaagaa                                                                                                                                                <  1:2951220/150‑1 (MQ=255)
     atgatAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGgaagaa                                                                                                                                                >  1:3636816/1‑150 (MQ=255)
         tAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAg                                                                                                                                            <  2:2559560/150‑1 (MQ=255)
          aGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGt                                                                                                                                           <  2:3418292/150‑1 (MQ=255)
            ttATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACATTGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTc                                                                                                                                         <  2:2097528/150‑1 (MQ=255)
               tctatcATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTcgcg                                                                                                                                      >  1:1663727/1‑150 (MQ=255)
                 tatcATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCTGCGGCTGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGAc                                                                                                                                    <  2:3937000/150‑1 (MQ=255)
                  atcATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGAcg                                                                                                                                   <  1:1443284/150‑1 (MQ=255)
                    cATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGAcgcg                                                                                                                                 >  2:2857260/1‑150 (MQ=255)
                    cATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGAcgcg                                                                                                                                 >  1:374727/1‑150 (MQ=255)
                    attgtgGATTAGATTGGTCCGGCAAATAAGCTGTTGTCAGCGGCTGGGGGTTCGGCCGATAACCGTAACCGAATTTCATAACTGACATGGGTTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGAcgcg                                                                                                                                 <  1:2010245/148‑1 (MQ=255)
                     aTGTGGAGTAGTTTGGTCAGGCAAATAATCTCTTGTCAGCGGCAGGGCTTTCTGCCGATAACCGTAACCGTAGATGATAACTGACATTGGGTAAAACGATCGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACgcgc                                                                                                                                <  2:4425847/150‑1 (MQ=255)
                       gtgttttAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCTATAACCGTAACCGAATATGATAACTTACAATGGGTAAACCTAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGCCGCGCTa                                                                                                                              <  2:886854/144‑1 (MQ=255)
                       gtgGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCTATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTa                                                                                                                              <  1:4479022/150‑1 (MQ=255)
                       gtgGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTa                                                                                                                              >  2:3620303/1‑150 (MQ=255)
                          gAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTaaaa                                                                                                                           >  1:3941815/1‑150 (MQ=255)
                            gTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAAcc                                                                                                                         <  2:3970620/150‑1 (MQ=255)
                               gATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCTATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGcc                                                                                                                      <  1:3760616/150‑1 (MQ=255)
                                   ggTCAGGCAAATAAGCTCTTGTCAGCGGCTGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCt                                                                                                                  <  2:536084/150‑1 (MQ=255)
                                     tctggaaaaTAAGCTCTTGTCAGCGGCAGGGCTTTCTGCCGATAACCGTAACCTAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTAt                                                                                                                <  1:2406293/144‑1 (MQ=255)
                                     tCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTAt                                                                                                                <  1:2095746/150‑1 (MQ=255)
                                     tCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTAt                                                                                                                <  1:3456698/150‑1 (MQ=255)
                                          cAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGtata                                                                                                           <  2:1103489/150‑1 (MQ=255)
                                            aaTAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATaaa                                                                                                         >  2:2688071/1‑150 (MQ=255)
                                              tAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGCACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGt                                                                                                       <  2:1620442/150‑1 (MQ=255)
                                               aaGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTg                                                                                                      >  2:4607247/1‑150 (MQ=255)
                                                aGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGa                                                                                                     <  1:4528645/150‑1 (MQ=255)
                                                  ctctTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGata                                                                                                   >  1:2389372/1‑150 (MQ=255)
                                                  ctctTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGata                                                                                                   >  2:677629/1‑150 (MQ=255)
                                                  ctctTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGata                                                                                                   <  1:1517731/150‑1 (MQ=255)
                                                   tctTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGatat                                                                                                  <  2:1512270/150‑1 (MQ=255)
                                                     ttGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTa                                                                                                <  2:1423902/150‑1 (MQ=255)
                                                      tGTCCGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAg                                                                                               <  1:1344053/150‑1 (MQ=255)
                                                       gTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGACTATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGt                                                                                              <  1:4102373/150‑1 (MQ=255)
                                                        tCAGCGGCTGGGCGTTCTGCCTATAACCGTAACCGAAGATGATAACTTACAAGGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTc                                                                                             <  1:1251311/150‑1 (MQ=255)
                                                         cAGCGGCTGGGCGTTCTGCCTATAACCGTAACCGAAGATGATAACTGACAATGGTTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCa                                                                                            <  1:862034/150‑1 (MQ=255)
                                                             ggCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAatga                                                                                        >  2:919684/1‑150 (MQ=255)
                                                              gctgggCGTTCTGCCGATACCCGTAACCGAAGATGATAAGTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAatgat                                                                                       <  1:3533334/147‑1 (MQ=255)
                                                              gccgggCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAatgat                                                                                       <  1:4159224/147‑1 (MQ=255)
                                                                aGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAAtgatga                                                                                     <  1:4554559/150‑1 (MQ=255)
                                                                 gggCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATgatgat                                                                                    >  2:1253190/1‑150 (MQ=255)
                                                                     gTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTaca                                                                                >  2:1863163/1‑150 (MQ=255)
                                                                          gCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAGGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCg                                                                           <  2:760244/150‑1 (MQ=255)
                                                                          gCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCg                                                                           >  2:61409/1‑150 (MQ=255)
                                                                           ccGATAACCTTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGa                                                                          <  1:4072789/150‑1 (MQ=255)
                                                                             gATAACCGTAACCGAAGATGATAACTGACAATGGTTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATg                                                                        <  1:2528392/150‑1 (MQ=255)
                                                                              aTAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATgg                                                                       >  2:1673562/1‑150 (MQ=255)
                                                                               taaccgtaaccgAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGa                                                                      >  1:231572/1‑150 (MQ=255)
                                                                                     taaccgAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGt                                                                >  1:1970261/1‑150 (MQ=255)
                                                                                      aaccgAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGtt                                                               >  1:2205406/1‑150 (MQ=255)
                                                                                      aaccgAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGtt                                                               >  1:258988/1‑150 (MQ=255)
                                                                                       accgAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTa                                                              >  1:2319924/1‑150 (MQ=255)
                                                                                         cgAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTAtt                                                            <  2:4287431/150‑1 (MQ=255)
                                                                                         cgAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTAtt                                                            >  1:221213/1‑150 (MQ=255)
                                                                                         cgAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTAtt                                                            <  1:1033724/150‑1 (MQ=255)
                                                                                          gAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTg                                                           <  2:374746/150‑1 (MQ=255)
                                                                                               tgatAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACgtg                                                      <  2:1308330/150‑1 (MQ=255)
                                                                                               tgatAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACgtg                                                      <  1:2766187/150‑1 (MQ=255)
                                                                                                gatAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACgtgt                                                     >  2:1449542/1‑150 (MQ=255)
                                                                                                gatAACTGAAAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGAAGATTACACTCCGATGGAGTTTGTTAGTTACttgt                                                     >  2:320910/1‑150 (MQ=255)
                                                                                                 atAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGtt                                                    >  2:2990439/1‑150 (MQ=255)
                                                                                                 atAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGtt                                                    <  2:2648107/150‑1 (MQ=255)
                                                                                                  tAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTa                                                   >  1:3596588/1‑150 (MQ=255)
                                                                                                  tAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTa                                                   <  1:526893/150‑1 (MQ=255)
                                                                                                    aCTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACa                                                 >  2:2461687/1‑150 (MQ=255)
                                                                                                    aCTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACa                                                 >  2:2730235/1‑150 (MQ=255)
                                                                                                         cAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAt                                            <  1:3082109/150‑1 (MQ=255)
                                                                                                         cAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAt                                            <  1:68516/150‑1 (MQ=255)
                                                                                                         cAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAt                                            <  1:2973294/150‑1 (MQ=255)
                                                                                                         cAAGGGTTAAAACGCACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAt                                            <  1:1958768/150‑1 (MQ=255)
                                                                                                             gggTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttctt                                        <  2:3087890/150‑1 (MQ=255)
                                                                                                                tAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttcttttc                                     <  1:1877206/150‑1 (MQ=255)
                                                                                                                tAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCAGCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttcttttc                                     >  1:1079667/1‑150 (MQ=255)
                                                                                                                 aaaaCGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGTGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttcttttct                                    >  1:3052574/1‑150 (MQ=255)
                                                                                                                  aaaCGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttcttttctt                                   <  2:2516002/150‑1 (MQ=255)
                                                                                                                     cgaacgaCTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTatg                                >  1:237786/1‑150 (MQ=255)
                                                                                                                      gaacgaCTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTatga                               >  2:2199307/1‑150 (MQ=255)
                                                                                                                          gaCTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTa                           >  1:2755725/1‑150 (MQ=255)
                                                                                                                           aCTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAg                          <  2:4622343/150‑1 (MQ=255)
                                                                                                                           aCTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAg                          <  1:2359865/150‑1 (MQ=255)
                                                                                                                            ctggctggACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGa                         <  1:488088/150‑1 (MQ=255)
                                                                                                                            ctggctggACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGa                         >  1:2596971/1‑150 (MQ=255)
                                                                                                                              ggctggACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAAc                       >  2:119767/1‑150 (MQ=255)
                                                                                                                                 tggACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTg                    <  1:2961794/150‑1 (MQ=255)
                                                                                                                                 tggACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACTTg                    >  1:2030453/1‑150 (MQ=255)
                                                                                                                                 tggACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTg                    >  1:3395083/1‑150 (MQ=255)
                                                                                                                                  ggACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGc                   <  1:1743727/150‑1 (MQ=255)
                                                                                                                                    aCTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCaa                 <  2:2515967/150‑1 (MQ=255)
                                                                                                                                    aCTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCaa                 >  2:1547296/1‑150 (MQ=255)
                                                                                                                                        tGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTAGGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCa             <  1:1514120/150‑1 (MQ=255)
                                                                                                                                         gATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCaa            >  1:2165884/1‑150 (MQ=255)
                                                                                                                                          aTCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTAAGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACTCAAt           >  1:2697098/1‑150 (MQ=255)
                                                                                                                                           tCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAAtt          <  2:4145066/150‑1 (MQ=255)
                                                                                                                                            cAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAATTg         <  1:4325055/150‑1 (MQ=255)
                                                                                                                                                tGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAATTGATGc     >  2:3529763/1‑150 (MQ=255)
                                                                                                                                                 ggCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTGTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAATTGATGCt    >  1:4263948/1‑150 (MQ=255)
                                                                                                                                                   cGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAATTGATGCTCg  >  2:4368111/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAATTGATGCTCG  >  CP009273/918262‑918558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A