Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 972,542 C→A 32.4% S254* (TCG→TAG)  mukB → chromosome condensin MukBEF, ATPase and DNA‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273972,5420CA32.4% 504.1 / 218.8 260S254* (TCG→TAG) mukBchromosome condensin MukBEF, ATPase and DNA‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (78/97);  new base A (45/40);  total (123/137)
Fisher's exact test for biased strand distribution p-value = 2.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

GCTGTATGGCGGGATCTCCAGTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGC  >  CP009273/972393‑972689
                                                                                                                                                     |                                                                                                                                                   
gCTGTATGGCGGGATCTCCAGTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTc                                                                                                                                                     <  1:4142329/150‑1 (MQ=255)
gCTGTATGGCGGGATCTCCAGTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTc                                                                                                                                                     <  2:1963233/150‑1 (MQ=255)
 cTGTATGGCGGGATCTCCAGTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCg                                                                                                                                                    >  1:1146774/1‑150 (MQ=255)
  tGTATGGCGGGATCTCCAGTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCgg                                                                                                                                                   <  2:646250/150‑1 (MQ=255)
  tGTATGGCGGGATCTCCAGTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAgg                                                                                                                                                   >  1:107428/1‑150 (MQ=255)
      tGGCGGGATCTCCAGTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACcg                                                                                                                                               <  1:2635804/150‑1 (MQ=255)
        gCGGGATCTCCAGTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACcgcg                                                                                                                                             <  2:151816/150‑1 (MQ=255)
        gCGGGATCTCCAGTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACcgcg                                                                                                                                             <  1:3611220/150‑1 (MQ=255)
               ctcCAGTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGtt                                                                                                                                      >  1:4217724/1‑150 (MQ=255)
               ctcCAGTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGtt                                                                                                                                      <  1:3181832/150‑1 (MQ=255)
                 ccAGTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTa                                                                                                                                    <  1:4496892/150‑1 (MQ=255)
                  cAGTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTaa                                                                                                                                   >  2:1416493/1‑150 (MQ=255)
                   aGTGCCATTACCCGTTCTCTGCGCGACTACCTGGTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAg                                                                                                                                  <  2:2189782/150‑1 (MQ=255)
                    gTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGc                                                                                                                                 >  2:3776070/1‑150 (MQ=255)
                     tGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCCGTAGGACCGCGACCTGTTTAAGCa                                                                                                                                >  2:4188204/1‑150 (MQ=255)
                      gCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCAt                                                                                                                               >  1:3810562/1‑150 (MQ=255)
                       ccATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATc                                                                                                                              <  1:2156162/150‑1 (MQ=255)
                        cATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCt                                                                                                                             <  2:366129/150‑1 (MQ=255)
                           tACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGAt                                                                                                                          >  1:60229/1‑150 (MQ=255)
                           tACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGAt                                                                                                                          >  2:393335/1‑150 (MQ=255)
                            aCCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATc                                                                                                                         <  2:4246294/150‑1 (MQ=255)
                            aCCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATc                                                                                                                         >  2:317598/1‑150 (MQ=255)
                            aCCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATc                                                                                                                         >  2:2767756/1‑150 (MQ=255)
                             cccGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCTGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCa                                                                                                                        <  2:4448079/150‑1 (MQ=255)
                             cccGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCa                                                                                                                        <  2:2560833/150‑1 (MQ=255)
                             cccGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCa                                                                                                                        <  2:246011/150‑1 (MQ=255)
                               cGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGc                                                                                                                      <  2:694453/150‑1 (MQ=255)
                               cGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGc                                                                                                                      <  1:3359833/150‑1 (MQ=255)
                                 ttCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGa                                                                                                                    <  2:3884487/150‑1 (MQ=255)
                                   ctctGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAg                                                                                                                  <  1:2197762/150‑1 (MQ=255)
                                   ctctGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAg                                                                                                                  >  2:2700693/1‑150 (MQ=255)
                                              tACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTAc                                                                                                       >  2:551522/1‑150 (MQ=255)
                                              tACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTAc                                                                                                       <  2:139454/150‑1 (MQ=255)
                                               aCCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACg                                                                                                      >  1:1263138/1‑150 (MQ=255)
                                                 cTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTg                                                                                                    >  2:2102361/1‑150 (MQ=255)
                                                 cTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTg                                                                                                    >  2:4406544/1‑150 (MQ=255)
                                                   gTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTggc                                                                                                  <  1:1759901/150‑1 (MQ=255)
                                                    ttGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTggcg                                                                                                 <  2:432564/150‑1 (MQ=255)
                                                      gCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTggcggc                                                                                               <  2:1806986/150‑1 (MQ=255)
                                                      gCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTggcggc                                                                                               <  2:3368481/150‑1 (MQ=255)
                                                      gCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTggcggc                                                                                               <  1:1021295/150‑1 (MQ=255)
                                                       ccAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGgcggcg                                                                                              >  1:3329860/1‑150 (MQ=255)
                                                        cAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGcggcgg                                                                                             >  2:3258335/1‑150 (MQ=255)
                                                         aGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGa                                                                                            >  1:96664/1‑150 (MQ=255)
                                                           aaaaCAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACt                                                                                          >  1:3477773/1‑150 (MQ=255)
                                                            aaaCAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTa                                                                                         <  2:2735773/150‑1 (MQ=255)
                                                            aaaCAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTa                                                                                         >  2:2292433/1‑150 (MQ=255)
                                                            aaaCAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTa                                                                                         >  2:4146350/1‑150 (MQ=255)
                                                            aaaCAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTa                                                                                         >  1:3978586/1‑150 (MQ=255)
                                                              aCAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACa                                                                                       >  1:1430494/1‑150 (MQ=255)
                                                                aGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATg                                                                                     <  1:4080206/150‑1 (MQ=255)
                                                                   ggcgTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGt                                                                                  >  1:2772929/1‑150 (MQ=255)
                                                                   ggcgTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGt                                                                                  <  2:1315421/150‑1 (MQ=255)
                                                                    gcgtgcgtAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTc                                                                                 >  1:1728037/1‑150 (MQ=255)
                                                                    gcgtgcgtAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTc                                                                                 <  2:79447/150‑1 (MQ=255)
                                                                     cgtgcgtAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCa                                                                                >  2:767153/1‑150 (MQ=255)
                                                                       tgcgtAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACg                                                                              >  1:51612/1‑150 (MQ=255)
                                                                       tgcgtAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACg                                                                              >  1:3691774/1‑150 (MQ=255)
                                                                           tAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCaa                                                                          >  1:4304303/1‑150 (MQ=255)
                                                                            aaaGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAAc                                                                         <  1:3705954/150‑1 (MQ=255)
                                                                            aaaGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAAc                                                                         <  1:840391/150‑1 (MQ=255)
                                                                            aaaGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAAc                                                                         <  2:2584365/150‑1 (MQ=255)
                                                                              aGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGa                                                                       >  2:167353/1‑150 (MQ=255)
                                                                                  ttCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAgcgc                                                                   >  1:1403240/1‑150 (MQ=255)
                                                                                     cAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCgt                                                                >  2:2343086/1‑150 (MQ=255)
                                                                                     cAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCgt                                                                <  1:462921/150‑1 (MQ=255)
                                                                                      aGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCgtg                                                               >  2:259118/1‑150 (MQ=255)
                                                                                       ggACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCgtgt                                                              <  1:840943/150‑1 (MQ=255)
                                                                                         aCATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTcc                                                            >  2:672628/1‑150 (MQ=255)
                                                                                           aTGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCAt                                                          >  1:1953812/1‑150 (MQ=255)
                                                                                              gAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCAtctc                                                       <  1:3475634/150‑1 (MQ=255)
                                                                                                      gcTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGc                                               <  2:2742625/150‑1 (MQ=255)
                                                                                                             gAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAg                                        <  1:2824009/150‑1 (MQ=255)
                                                                                                             gAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAg                                        <  1:207952/150‑1 (MQ=255)
                                                                                                              aaaaCCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGt                                       >  2:4081725/1‑150 (MQ=255)
                                                                                                                  ccGTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTtcg                                   >  1:1729335/1‑150 (MQ=255)
                                                                                                                    gTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTtcgtc                                 >  1:723836/1‑150 (MQ=255)
                                                                                                                    gTATGACGCTGGAAGCGATTCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTtcgtc                                 <  2:3863980/150‑1 (MQ=255)
                                                                                                                      aTGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTcgc                               >  2:3149972/1‑150 (MQ=255)
                                                                                                                           gCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCt                          >  1:24859/1‑150 (MQ=255)
                                                                                                                             tGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTAc                        >  2:924785/1‑150 (MQ=255)
                                                                                                                               gAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACAt                      >  1:3892875/1‑150 (MQ=255)
                                                                                                                                   cGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACtt                  <  2:3047797/150‑1 (MQ=255)
                                                                                                                                    gATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTc                 >  2:3489765/1‑150 (MQ=255)
                                                                                                                                    gATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTc                 >  1:1654020/1‑150 (MQ=255)
                                                                                                                                      ttCGTGTCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTAGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACttagt               <  2:2077999/150‑4 (MQ=255)
                                                                                                                                        cGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGt             <  1:3991673/150‑1 (MQ=255)
                                                                                                                                         gtgtCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTc            <  1:2921257/150‑1 (MQ=255)
                                                                                                                                         gtgtCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTc            >  1:2406285/1‑150 (MQ=255)
                                                                                                                                         gtgtCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTc            <  1:1358619/150‑1 (MQ=255)
                                                                                                                                         gtgtCACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTc            <  2:3086112/150‑1 (MQ=255)
                                                                                                                                             cACCCAGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCa        <  1:3264547/150‑1 (MQ=255)
                                                                                                                                                ccAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACt     <  2:1146774/150‑1 (MQ=255)
                                                                                                                                                 cAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTg    <  1:3120618/150‑1 (MQ=255)
                                                                                                                                                  aGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTgg   >  1:1607868/1‑150 (MQ=255)
                                                                                                                                                  aGTAGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTgg   >  2:2886037/1‑150 (MQ=255)
                                                                                                                                                   gTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTggc  >  1:2014045/1‑150 (MQ=255)
                                                                                                                                                   gTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTggc  <  1:1561717/150‑1 (MQ=255)
                                                                                                                                                   gTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTggc  >  2:2332434/1‑150 (MQ=255)
                                                                                                                                                   gTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTggc  >  1:4267004/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCTGTATGGCGGGATCTCCAGTGCCATTACCCGTTCTCTGCGCGACTACCTGTTGCCAGAAAACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGC  >  CP009273/972393‑972689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A