Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,246,435 T→A 48.3% intergenic (‑141/‑87) dhaK ← / → dhaR dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit/dhaKLM operon transcription activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,246,4350TA48.3% 26.8 / inf 209intergenic (‑141/‑87)dhaK/dhaRdihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit/dhaKLM operon transcription activator
Reads supporting (aligned to +/- strand):  ref base T (58/50);  new base A (49/52);  total (107/102)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

TTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGG  >  CP009273/1246286‑1246582
                                                                                                                                                     |                                                                                                                                                   
  ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTaaa                                                                                                                                                   >  2:949121/1‑150 (MQ=255)
  ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTaaa                                                                                                                                                   >  2:934616/1‑150 (MQ=255)
  ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTaaa                                                                                                                                                   >  2:3272353/1‑150 (MQ=255)
      cATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAaatgaa                                                                                                                                               <  2:3708319/150‑1 (MQ=255)
       attattTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTaatgaat                                                                                                                                              >  2:1153262/1‑150 (MQ=255)
         tattTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGt                                                                                                                                            >  1:823336/1‑150 (MQ=255)
            tttGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTcc                                                                                                                                         >  2:3608158/1‑150 (MQ=255)
            tttGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTcg                                                                                                                                         <  1:2809998/150‑2 (MQ=255)
            tttGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTcg                                                                                                                                         <  1:1382891/150‑2 (MQ=255)
            tttGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTcc                                                                                                                                         <  1:4193503/150‑1 (MQ=255)
               gCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCata                                                                                                                                      <  2:201395/150‑1 (MQ=255)
                 tCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATAtt                                                                                                                                    <  1:3964817/150‑1 (MQ=255)
                   cAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGa                                                                                                                                  >  1:2475414/1‑150 (MQ=255)
                    aGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGaa                                                                                                                                 >  2:2479048/1‑150 (MQ=255)
                    aGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGaa                                                                                                                                 <  2:1972160/150‑1 (MQ=255)
                      taaTTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACGACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAAc                                                                                                                               >  2:4303012/2‑150 (MQ=255)
                       aaTTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACt                                                                                                                              >  2:1622260/1‑150 (MQ=255)
                         ttACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACttt                                                                                                                            <  1:3485997/150‑1 (MQ=255)
                          tACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACtttt                                                                                                                           <  2:4219435/150‑1 (MQ=255)
                            cGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTAc                                                                                                                         <  1:2054657/150‑1 (MQ=255)
                            cGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTAc                                                                                                                         <  1:3023541/150‑1 (MQ=255)
                             ggTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                                                                        <  1:1925923/150‑1 (MQ=255)
                              gTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTActt                                                                                                                       >  2:4014560/1‑148 (MQ=255)
                                  ggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAt                                                                                                                   <  2:1012747/150‑1 (MQ=255)
                                  ggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAt                                                                                                                   >  1:2445122/1‑150 (MQ=255)
                                   gCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                                                                  <  2:1324744/150‑1 (MQ=255)
                                     aTGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTaa                                                                                                                <  1:2078196/150‑1 (MQ=255)
                                      tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAAt                                                                                                               <  1:2241997/150‑1 (MQ=255)
                                      tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAAt                                                                                                               >  2:3677455/1‑150 (MQ=255)
                                        gatgatGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATAc                                                                                                             >  2:4392462/1‑150 (MQ=255)
                                         atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                                                                            <  1:1323460/150‑1 (MQ=255)
                                          tgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACtt                                                                                                           >  2:3098765/1‑150 (MQ=255)
                                               ttCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAAt                                                                                                      >  1:2200479/1‑150 (MQ=255)
                                               ttCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAAt                                                                                                      >  2:3363476/1‑150 (MQ=255)
                                                    cGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGa                                                                                                 <  2:4521179/150‑1 (MQ=255)
                                                    cGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGa                                                                                                 <  2:2149624/150‑1 (MQ=255)
                                                     gACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAg                                                                                                >  1:4593324/1‑150 (MQ=255)
                                                       cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGcc                                                                                              <  1:3321939/150‑1 (MQ=255)
                                                        acGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCg                                                                                             <  1:119477/150‑1 (MQ=255)
                                                             gACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAaca                                                                                        >  1:411424/1‑150 (MQ=255)
                                                             gACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAaca                                                                                        >  2:3705317/1‑150 (MQ=255)
                                                                cACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCt                                                                                     <  1:2925849/150‑1 (MQ=255)
                                                                 actgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                                                                    <  1:1093660/150‑1 (MQ=255)
                                                                  ctgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCttt                                                                                   >  1:4401744/1‑150 (MQ=255)
                                                                   tgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtttt                                                                                  >  1:2097104/1‑150 (MQ=255)
                                                                   tgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtttt                                                                                  <  2:4429102/150‑1 (MQ=255)
                                                                      ctgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTc                                                                               >  2:2133884/1‑150 (MQ=255)
                                                                        gCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                                                                             <  1:1466729/150‑1 (MQ=255)
                                                                        gCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                                                                             >  2:549683/1‑150 (MQ=255)
                                                                            aTGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAgg                                                                         <  1:2479048/150‑1 (MQ=255)
                                                                             tGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAggg                                                                        <  2:4158836/150‑1 (MQ=255)
                                                                              gAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                                                                       <  1:1007085/150‑1 (MQ=255)
                                                                                  ccATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCg                                                                   >  1:681140/1‑150 (MQ=255)
                                                                                   cATTGTGCATCAGTCAATGGAAAAGAAAATTAGCCCAAATTATTGTTCATAGTGACACATATGCTTTATTGAATGTTCCATATTGAAACTTTTACGTGTATGAAGACTTAACATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCgg                                                                  <  2:4034658/150‑1 (MQ=255)
                                                                                   cATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCgg                                                                  <  1:833155/150‑1 (MQ=255)
                                                                                      tgtgCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat                                                               >  2:3878498/1‑150 (MQ=255)
                                                                                       gtgCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGata                                                              <  1:2865291/150‑1 (MQ=255)
                                                                                            tCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGt                                                         >  1:3703402/1‑150 (MQ=255)
                                                                                                   aGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCtt                                                  <  2:3948804/150‑1 (MQ=255)
                                                                                                   aGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCtt                                                  <  1:64064/150‑1 (MQ=255)
                                                                                                      gaaaagaaaaTTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTa                                               >  2:1558072/1‑150 (MQ=255)
                                                                                                       aaaagaaaaTTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTaa                                              <  2:1842448/150‑1 (MQ=255)
                                                                                                       aaaagaaaaTTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTaa                                              >  1:1802556/1‑150 (MQ=255)
                                                                                                       aaaagaaaaTTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCAGATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTaa                                              >  1:4458491/1‑150 (MQ=255)
                                                                                                        aaagaaaaTTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTaac                                             >  2:3331106/1‑150 (MQ=255)
                                                                                                          agaaaaTTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTaacaa                                           >  1:2767724/1‑150 (MQ=255)
                                                                                                            aaaaTTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACg                                         >  2:4594325/1‑150 (MQ=255)
                                                                                                            aaaaTTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACg                                         >  2:1760447/1‑150 (MQ=255)
                                                                                                              aaTTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGAt                                       <  2:20517/150‑1 (MQ=255)
                                                                                                               aTTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATg                                      <  2:851358/150‑1 (MQ=255)
                                                                                                                 tAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGt                                    <  1:930624/150‑1 (MQ=255)
                                                                                                                   gCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCg                                  <  2:2974819/150‑1 (MQ=255)
                                                                                                                   gCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCg                                  <  2:4630980/150‑1 (MQ=255)
                                                                                                                    cccAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCgg                                 >  1:4380885/1‑150 (MQ=255)
                                                                                                                     ccAAATTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCggg                                <  1:3497331/150‑1 (MQ=255)
                                                                                                                      cAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCgggg                               <  1:3570030/150‑1 (MQ=255)
                                                                                                                      cAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCgggg                               >  1:1861916/1‑150 (MQ=255)
                                                                                                                       aaaTTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGc                              <  1:549683/150‑1 (MQ=255)
                                                                                                                        aaTTATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCTATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCa                             >  2:2896668/1‑150 (MQ=255)
                                                                                                                          ttATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCata                           <  2:878687/150‑1 (MQ=255)
                                                                                                                           tATGTTTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCatat                          >  2:2829372/1‑150 (MQ=255)
                                                                                                                               tttCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGTATGCGGGATATTAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTcc                      <  2:2638422/150‑1 (MQ=255)
                                                                                                                                  cATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCtt                   >  1:3245530/1‑150 (MQ=255)
                                                                                                                                  cATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCtt                   >  2:3735008/1‑150 (MQ=255)
                                                                                                                                   aTAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTa                  >  1:1438646/1‑150 (MQ=255)
                                                                                                                                   aTAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTa                  <  2:3754071/150‑1 (MQ=255)
                                                                                                                                    tAGTGAAACATATGCTTTAAGGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTGTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTaa                 >  2:2795497/1‑150 (MQ=255)
                                                                                                                                     aGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAAt                <  2:3949598/150‑1 (MQ=255)
                                                                                                                                      gTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAAtt               <  1:2531559/150‑1 (MQ=255)
                                                                                                                                      gTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAAtt               <  2:3568158/150‑1 (MQ=255)
                                                                                                                                          aaCATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCaa           >  2:182072/1‑150 (MQ=255)
                                                                                                                                          aaCATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCaa           <  1:949121/150‑1 (MQ=255)
                                                                                                                                          aaCATATGCTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTGTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCaa           >  2:253688/1‑150 (MQ=255)
                                                                                                                                           aCATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAAc          >  2:3811505/1‑150 (MQ=255)
                                                                                                                                              tatGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAAcctc       >  1:1745830/1‑150 (MQ=255)
                                                                                                                                               atGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAAcctcc      >  2:397696/1‑150 (MQ=255)
                                                                                                                                                tGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAAcctcct     >  2:489113/1‑150 (MQ=255)
                                                                                                                                                  cTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAACCTCCTgg   >  1:1502745/1‑150 (MQ=255)
                                                                                                                                                  cTTAAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAACCTCCTgg   <  2:1233339/150‑1 (MQ=255)
                                                                                                                                                   tttAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAACCTCCTggg  <  1:2361416/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGG  >  CP009273/1246286‑1246582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A