Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,481,236 G→T 17.0% intergenic (+16/‑256) hrpA → / → ydcF putative ATP‑dependent helicase/DUF218 superfamily protein, SAM‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,481,2360GT17.0% 846.7 / 110.5 276intergenic (+16/‑256)hrpA/ydcFputative ATP‑dependent helicase/DUF218 superfamily protein, SAM‑binding
Reads supporting (aligned to +/- strand):  ref base G (110/119);  new base T (26/21);  total (136/140)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

Only 100 of 279 total aligned reads displayed.

GGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCA  >  CP009273/1481088‑1481385
                                                                                                                                                    |                                                                                                                                                     
 gATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTTAt                                                                                                                                                     <  1:4020707/150‑1 (MQ=255)
  aTGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATa                                                                                                                                                    <  2:4555548/150‑1 (MQ=255)
  aTGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATa                                                                                                                                                    >  1:965463/1‑150 (MQ=255)
     aaGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAg                                                                                                                                                 >  2:892934/1‑150 (MQ=255)
     aaGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAg                                                                                                                                                 >  2:530147/1‑150 (MQ=255)
      aGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGa                                                                                                                                                <  2:389981/150‑1 (MQ=255)
       gACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGaa                                                                                                                                               <  2:1006692/150‑1 (MQ=255)
            gAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAAc                                                                                                                                          >  1:3957965/1‑150 (MQ=255)
             aaaGAGATCCTTTGTATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATACGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAAcc                                                                                                                                         <  2:3201523/150‑1 (MQ=255)
             aaaGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAAcc                                                                                                                                         >  2:668635/1‑150 (MQ=255)
             aaaGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAAcc                                                                                                                                         >  2:3476910/1‑150 (MQ=255)
                  gaTCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTa                                                                                                                                    >  2:3165746/1‑150 (MQ=255)
                   aTCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTaa                                                                                                                                   <  2:3144181/150‑1 (MQ=255)
                      cGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCa                                                                                                                                <  1:626278/150‑1 (MQ=255)
                       gTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCAt                                                                                                                               <  2:1182702/150‑1 (MQ=255)
                        ttGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCAtt                                                                                                                              <  1:1154050/150‑1 (MQ=255)
                         tGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCAttt                                                                                                                             >  1:2082938/1‑150 (MQ=255)
                          ggATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTa                                                                                                                            <  1:2373770/150‑1 (MQ=255)
                          ggATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTa                                                                                                                            <  2:4014570/150‑1 (MQ=255)
                                 agaagaGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCgg                                                                                                                     <  2:3769439/150‑1 (MQ=255)
                                   aagaGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGt                                                                                                                   <  1:2933878/150‑1 (MQ=255)
                                    agagTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGtt                                                                                                                  >  2:2356662/1‑150 (MQ=255)
                                      agTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGtttt                                                                                                                >  1:1250979/1‑150 (MQ=255)
                                      agTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGtttt                                                                                                                >  1:668127/1‑150 (MQ=255)
                                      agTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGtttt                                                                                                                <  1:2354712/150‑1 (MQ=255)
                                      agTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGtttt                                                                                                                <  2:598476/150‑1 (MQ=255)
                                       gTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGttttt                                                                                                               <  1:44974/150‑1 (MQ=255)
                                       gTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGttttt                                                                                                               <  1:45188/150‑1 (MQ=255)
                                           cgcgTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTttatt                                                                                                           >  2:4195070/1‑150 (MQ=255)
                                             cgTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTaa                                                                                                         >  2:1336403/1‑150 (MQ=255)
                                                    taCTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCTGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTaaa                                                                                                  <  1:546392/150‑1 (MQ=255)
                                                      cTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAAc                                                                                                >  1:337025/1‑150 (MQ=255)
                                                       ttCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACg                                                                                               <  1:3082974/150‑1 (MQ=255)
                                                        tCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACgg                                                                                              >  1:227600/1‑150 (MQ=255)
                                                        tCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACgg                                                                                              <  1:3339886/150‑1 (MQ=255)
                                                        tCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACgg                                                                                              >  2:274699/1‑150 (MQ=255)
                                                           cTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTaa                                                                                           >  2:2931100/1‑150 (MQ=255)
                                                           cTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTaa                                                                                           <  1:2008083/150‑1 (MQ=255)
                                                            tCAACAACTTGGTATGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAg                                                                                          <  1:290879/150‑1 (MQ=255)
                                                            tCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAg                                                                                          >  1:3795907/1‑150 (MQ=255)
                                                            tCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAg                                                                                          >  2:3882766/1‑150 (MQ=255)
                                                             caacaaCTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAgg                                                                                         >  2:2032249/1‑150 (MQ=255)
                                                              aacaacTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAggg                                                                                        <  1:2491976/150‑1 (MQ=255)
                                                                 aacTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTaa                                                                                     >  1:1759740/1‑150 (MQ=255)
                                                                  acTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTaaa                                                                                    <  1:955095/150‑1 (MQ=255)
                                                                      ggTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAAtt                                                                                >  1:3409904/1‑150 (MQ=255)
                                                                      ggTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAAtt                                                                                <  2:2100428/150‑1 (MQ=255)
                                                                       gTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTc                                                                               >  1:3826643/1‑150 (MQ=255)
                                                                        tACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCa                                                                              >  1:2746404/1‑150 (MQ=255)
                                                                          cGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAgg                                                                            <  2:1473042/150‑1 (MQ=255)
                                                                          cGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAgg                                                                            <  1:4083039/150‑1 (MQ=255)
                                                                             cTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAAt                                                                         <  1:4565092/150‑1 (MQ=255)
                                                                             cTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAAt                                                                         <  2:2641191/150‑1 (MQ=255)
                                                                              ttATCCGATATCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAAtt                                                                        <  1:2169280/150‑1 (MQ=255)
                                                                               tATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTc                                                                       <  1:3170810/150‑1 (MQ=255)
                                                                               tATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTc                                                                       >  2:1848263/1‑150 (MQ=255)
                                                                                 tCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAATACCCGGTAAGCATTTAGCGCCGGGTTTTTTTTTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAg                                                                     <  2:1910250/150‑1 (MQ=255)
                                                                                   cGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGaa                                                                   >  1:821988/1‑150 (MQ=255)
                                                                                    gATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGaaa                                                                  >  2:244836/1‑150 (MQ=255)
                                                                                     aTTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGACTTCAGaaaa                                                                 >  2:2513380/1‑150 (MQ=255)
                                                                                       ttCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTTCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCGGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAAt                                                               <  1:2432414/150‑1 (MQ=255)
                                                                                       ttCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAAt                                                               <  2:2164779/150‑1 (MQ=255)
                                                                                       ttCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAAt                                                               <  2:2271795/150‑1 (MQ=255)
                                                                                        tCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATa                                                              >  1:1697870/1‑150 (MQ=255)
                                                                                         cAGATAAGCGTATTTTGCTGGCTATGGAGCAGATTAGCGGTTATCCCTGCTATTTGCCTGATAAAGAAAAAACCCGTTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATAc                                                             <  1:2969012/150‑1 (MQ=255)
                                                                                          aGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACa                                                            >  1:4328865/1‑150 (MQ=255)
                                                                                           gATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACaa                                                           >  2:2273324/1‑150 (MQ=255)
                                                                                           gATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGAAAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACaa                                                           <  1:707888/150‑1 (MQ=255)
                                                                                            aTAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAAt                                                          >  2:1057147/1‑150 (MQ=255)
                                                                                            aTAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAAt                                                          >  1:1935965/1‑150 (MQ=255)
                                                                                             tAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAAtt                                                         <  2:1424610/150‑1 (MQ=255)
                                                                                              aaGCGTATTTTGCAGGCGATGGATCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATtc                                                        <  2:4130952/150‑1 (MQ=255)
                                                                                               aGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATtct                                                       >  2:3041630/1‑150 (MQ=255)
                                                                                                 cGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTctct                                                     >  2:3631324/1‑150 (MQ=255)
                                                                                                  gTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTctg                                                    <  1:2189018/150‑1 (MQ=255)
                                                                                                   tATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTctgc                                                   >  2:774504/1‑150 (MQ=255)
                                                                                                    aTTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTctgct                                                  >  1:4464365/1‑150 (MQ=255)
                                                                                                       ttGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCa                                               <  1:3476910/150‑1 (MQ=255)
                                                                                                          cAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGa                                            >  1:3210586/1‑150 (MQ=255)
                                                                                                            ggCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATg                                          <  2:205224/150‑1 (MQ=255)
                                                                                                               gATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGaat                                       >  2:2590591/1‑150 (MQ=255)
                                                                                                                    aGCAGATTAGCGGTTAACCCTGCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGt                                  >  2:4338932/1‑150 (MQ=255)
                                                                                                                    aGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGt                                  >  2:1781801/1‑150 (MQ=255)
                                                                                                                     gCAGATTAGCGGTTAACCCTGCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGtt                                 <  1:2547923/150‑1 (MQ=255)
                                                                                                                       aGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTa                               >  1:2314930/1‑150 (MQ=255)
                                                                                                                       aGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTa                               >  1:485291/1‑150 (MQ=255)
                                                                                                                        gATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTAt                              >  2:2596016/1‑150 (MQ=255)
                                                                                                                         aTTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATc                             >  1:987765/1‑150 (MQ=255)
                                                                                                                          ttAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCt                            <  2:301839/150‑1 (MQ=255)
                                                                                                                           tAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTa                           <  2:3355766/150‑1 (MQ=255)
                                                                                                                              cGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAg                        >  2:2199473/1‑150 (MQ=255)
                                                                                                                                gTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCa                      <  2:1696323/150‑1 (MQ=255)
                                                                                                                                   aaCCCTGCTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCAttt                   >  1:383958/1‑150 (MQ=255)
                                                                                                                                        tGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTa              >  2:4443845/1‑150 (MQ=255)
                                                                                                                                         gCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTaa             <  2:2790756/150‑1 (MQ=255)
                                                                                                                                          cTATTTGCCTTATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTaaa            >  1:1581392/1‑150 (MQ=255)
                                                                                                                                                gCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGatat      <  1:3895961/150‑1 (MQ=255)
                                                                                                                                                   tGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTc   >  2:819210/1‑150 (MQ=255)
                                                                                                                                                   tGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTc   >  1:1398189/1‑150 (MQ=255)
                                                                                                                                                    gATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCa  >  1:2850859/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCA  >  CP009273/1481088‑1481385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A