Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,352,082 C→A 42.4% P81P (CCG→CCT)  rhmA ← 2‑keto‑3‑deoxy‑L‑rhamnonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,352,0820CA42.4% 280.5 / inf 276P81P (CCG→CCT) rhmA2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
Reads supporting (aligned to +/- strand):  ref base C (74/84);  new base A (46/71);  total (120/156)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 276 total aligned reads displayed.

CGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCT  >  CP009273/2351935‑2352229
                                                                                                                                                   |                                                                                                                                                   
cgcAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAgg                                                                                                                                                   <  1:969611/150‑1 (MQ=255)
 gcAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGt                                                                                                                                                  >  1:3175529/1‑150 (MQ=255)
  cAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGtt                                                                                                                                                 >  2:2882187/1‑150 (MQ=255)
    gCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGTAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTa                                                                                                                                               >  2:4176695/1‑150 (MQ=255)
    gCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTa                                                                                                                                               <  2:4447270/150‑1 (MQ=255)
       cGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTg                                                                                                                                            >  2:829574/1‑150 (MQ=255)
            cacaCTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTc                                                                                                                                       <  2:4358085/150‑1 (MQ=255)
             acacTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTcc                                                                                                                                      >  2:3994133/1‑150 (MQ=255)
                 tGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACcg                                                                                                                                  <  1:2958312/150‑1 (MQ=255)
                     cccGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACcggacg                                                                                                                              >  2:4291420/1‑150 (MQ=255)
                     cccGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACcggacg                                                                                                                              <  2:1699325/150‑1 (MQ=255)
                       cGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACcggacgga                                                                                                                            <  1:3935791/150‑1 (MQ=255)
                        gACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGAt                                                                                                                           >  1:4180782/1‑150 (MQ=255)
                        gACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGAt                                                                                                                           <  2:3737586/150‑1 (MQ=255)
                            ccacGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACg                                                                                                                       <  1:3959164/150‑1 (MQ=255)
                               cGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGt                                                                                                                    >  2:1905475/1‑150 (MQ=255)
                               cGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGt                                                                                                                    >  2:3837163/1‑150 (MQ=255)
                                  tCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTtgg                                                                                                                 >  1:1821159/1‑150 (MQ=255)
                                   cACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTtggc                                                                                                                <  1:2895271/150‑1 (MQ=255)
                                     ccGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTtggatg                                                                                                              <  1:4122311/150‑1 (MQ=255)
                                          gggAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATa                                                                                                         <  1:2298402/150‑1 (MQ=255)
                                              ggaTAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGgc                                                                                                     >  2:2456333/1‑150 (MQ=255)
                                              ggaTAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGgc                                                                                                     <  2:2521121/150‑1 (MQ=255)
                                                    cgcgTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTAcc                                                                                               <  2:2269029/150‑1 (MQ=255)
                                                    cgcgTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTAcc                                                                                               <  1:3556279/150‑1 (MQ=255)
                                                      cgTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGc                                                                                             <  1:3046855/150‑1 (MQ=255)
                                                       gTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGcc                                                                                            >  2:704626/1‑150 (MQ=255)
                                                       gTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGcc                                                                                            >  2:882957/1‑150 (MQ=255)
                                                        tGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCt                                                                                           <  2:2992418/150‑1 (MQ=255)
                                                        tGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCt                                                                                           >  1:1704068/1‑150 (MQ=255)
                                                          gCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCTCCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGt                                                                                         <  1:1292045/150‑1 (MQ=255)
                                                          gCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGt                                                                                         <  2:3174581/150‑1 (MQ=255)
                                                            aGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAg                                                                                       <  1:555671/150‑1 (MQ=255)
                                                            aGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAg                                                                                       <  1:1006989/150‑1 (MQ=255)
                                                            aGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAg                                                                                       <  2:4385275/150‑1 (MQ=255)
                                                               caccacCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtg                                                                                    <  2:987815/150‑1 (MQ=255)
                                                               caccacCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtg                                                                                    <  1:4469483/150‑1 (MQ=255)
                                                               caccacCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtg                                                                                    >  1:3680440/1‑150 (MQ=255)
                                                               caccacCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtg                                                                                    >  1:3368301/1‑150 (MQ=255)
                                                                accaccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtga                                                                                   >  2:3396888/1‑150 (MQ=255)
                                                                accaccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtga                                                                                   >  2:2717431/1‑150 (MQ=255)
                                                                  caccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtgatg                                                                                 >  2:2334517/1‑150 (MQ=255)
                                                                   accTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtgatga                                                                                >  2:4584631/1‑150 (MQ=255)
                                                                   accTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtgatga                                                                                <  1:2224976/150‑1 (MQ=255)
                                                                    ccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTgatgat                                                                               <  2:1404842/150‑1 (MQ=255)
                                                                    ccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTCGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTgatgat                                                                               <  1:1455745/150‑1 (MQ=255)
                                                                     cTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATa                                                                              <  1:1047948/150‑1 (MQ=255)
                                                                     cTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATa                                                                              <  1:2347295/150‑1 (MQ=255)
                                                                      tGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATaa                                                                             >  2:1540586/1‑150 (MQ=255)
                                                                       gACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATaaa                                                                            >  1:2566084/1‑150 (MQ=255)
                                                                          gTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGAt                                                                         >  2:4193772/1‑150 (MQ=255)
                                                                           tGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATc                                                                        <  1:3493004/150‑1 (MQ=255)
                                                                               tGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGa                                                                    >  1:1769529/1‑150 (MQ=255)
                                                                                gTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGaa                                                                   >  1:2057268/1‑150 (MQ=255)
                                                                                gTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGaa                                                                   <  2:3580477/150‑1 (MQ=255)
                                                                                   cGGCAGTATCGACCATCGGGATCAGTTGAGTTTGCGCGCCACTATCCATGAATTGTTTAATCAGGGGTTTACTGCCTTCCACCGGACGGATGAAGGGTTGGCTTGCATAGGGCGCTACCGCCTGTAGGTGATGATAATGATCCTGTATgg                                                                <  2:665965/150‑1 (MQ=255)
                                                                                   cGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATgg                                                                <  1:3851216/150‑1 (MQ=255)
                                                                                       aGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGtt                                                            <  1:2767131/150‑1 (MQ=255)
                                                                                       aGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGtt                                                            >  2:2509026/1‑150 (MQ=255)
                                                                                        gTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGttt                                                           <  1:253865/150‑1 (MQ=255)
                                                                                        gTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTAAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGttt                                                           >  1:3227626/1‑150 (MQ=255)
                                                                                         tATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTg                                                          >  1:1032526/1‑150 (MQ=255)
                                                                                              aCCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGcgcg                                                     <  2:975072/150‑1 (MQ=255)
                                                                                               ccATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGt                                                    >  2:2672293/1‑150 (MQ=255)
                                                                                                cATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTg                                                   >  1:970014/1‑150 (MQ=255)
                                                                                                cATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTAGCGCGTg                                                   >  2:2000984/1‑150 (MQ=255)
                                                                                                 aTCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGc                                                  >  1:725755/1‑150 (MQ=255)
                                                                                                    gggATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTcc                                               <  2:1351492/150‑1 (MQ=255)
                                                                                                     ggATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTccc                                              <  1:4066249/150‑1 (MQ=255)
                                                                                                      gATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTcccc                                             <  2:1613558/150‑1 (MQ=255)
                                                                                                       aTCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCg                                            <  1:1467057/150‑1 (MQ=255)
                                                                                                         cAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTc                                          >  1:1226054/1‑150 (MQ=255)
                                                                                                          aGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCa                                         <  2:502885/150‑1 (MQ=255)
                                                                                                          aGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCa                                         >  1:3889108/1‑150 (MQ=255)
                                                                                                            tAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAAt                                       <  1:2382424/150‑1 (MQ=255)
                                                                                                            tAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAAt                                       <  1:1732942/150‑1 (MQ=255)
                                                                                                             agagTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATc                                      >  1:1958433/1‑150 (MQ=255)
                                                                                                               agTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAg                                    <  2:3327403/150‑1 (MQ=255)
                                                                                                               agTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAg                                    >  2:3515363/1‑150 (MQ=255)
                                                                                                                  ttGCGCGCCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCaa                                 >  2:1191837/1‑150 (MQ=255)
                                                                                                                      gcgcCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAg                             <  1:1578312/150‑1 (MQ=255)
                                                                                                                       cgcCAATATCCAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGt                            >  1:2678499/1‑150 (MQ=255)
                                                                                                                        gcCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTc                           >  1:432039/1‑150 (MQ=255)
                                                                                                                            atatCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATaa                       >  2:2198527/1‑150 (MQ=255)
                                                                                                                              atCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAAcc                     >  1:2894427/1‑150 (MQ=255)
                                                                                                                                ccAGGACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAg                   >  1:4545607/1‑150 (MQ=255)
                                                                                                                                   ggACTTGTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAg                <  1:4584631/150‑1 (MQ=255)
                                                                                                                                     aCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTg              <  2:4047843/150‑1 (MQ=255)
                                                                                                                                      cTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTgg             <  2:511625/150‑1 (MQ=255)
                                                                                                                                       ttGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTggc            <  2:1250708/150‑1 (MQ=255)
                                                                                                                                        tGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTggcg           <  2:2544709/150‑1 (MQ=255)
                                                                                                                                         gTTTAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTggcgg          >  1:3649109/1‑150 (MQ=255)
                                                                                                                                          tttAATCATCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTggcggc         >  2:3687591/1‑150 (MQ=255)
                                                                                                                                           ttAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTNCTCCCCGTCNATCAGCAACCAGTCATAACCAGAAGTGGCGGCa        >  1:854199/1‑150 (MQ=255)
                                                                                                                                           ttAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGACTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCa        <  2:2390565/150‑1 (MQ=255)
                                                                                                                                            tAATCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCaa       <  1:2196255/150‑1 (MQ=255)
                                                                                                                                             aaTCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAAt      >  2:4141769/1‑150 (MQ=255)
                                                                                                                                               tCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAAttt    >  2:92599/1‑150 (MQ=255)
                                                                                                                                               tCAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAAttt    <  1:1903050/150‑1 (MQ=255)
                                                                                                                                                cAGAGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTc   >  1:2719071/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCT  >  CP009273/2351935‑2352229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A