Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,945,503 A→T 16.8% pseudogene (602/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,945,5030AT16.8% 714.7 / 130.7 268pseudogene (602/663 nt)ilvGpseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
Reads supporting (aligned to +/- strand):  ref base A (134/89);  new base T (18/27);  total (152/116)
Fisher's exact test for biased strand distribution p-value = 2.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

Only 100 of 269 total aligned reads displayed.

TAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACG  >  CP009273/3945357‑3945651
                                                                                                                                                  |                                                                                                                                                    
   ccccGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTg                                                                                                                                                >  2:892318/1‑150 (MQ=255)
    cccGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGATCGTCTgg                                                                                                                                               >  1:3358473/1‑150 (MQ=255)
    cccGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTgg                                                                                                                                               >  1:665256/1‑150 (MQ=255)
    cccGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTgg                                                                                                                                               >  1:2898456/1‑150 (MQ=255)
    cccGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTgg                                                                                                                                               >  1:378684/1‑150 (MQ=255)
        aTTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGc                                                                                                                                           >  1:43798/1‑150 (MQ=255)
         tttCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCt                                                                                                                                          <  1:1763230/150‑1 (MQ=255)
              tCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACCTCACCCGGACAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCAAGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTgc                                                                                                                                     >  1:228358/1‑150 (MQ=255)
               cATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTgcc                                                                                                                                    <  1:3933998/150‑1 (MQ=255)
                    tAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTg                                                                                                                               >  1:1660205/1‑150 (MQ=255)
                    tAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTg                                                                                                                               >  1:1875100/1‑150 (MQ=255)
                     aGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGATCGTCTGGCCGCTGGTGCCGCCTgg                                                                                                                              <  2:3859423/150‑1 (MQ=255)
                     aGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTgg                                                                                                                              >  1:419757/1‑150 (MQ=255)
                      gCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGgc                                                                                                                             <  2:2119287/150‑1 (MQ=255)
                       ccAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGATCGTCTGGCCGCTGGTGCCGCCTGgcg                                                                                                                            >  1:3157422/1‑150 (MQ=255)
                           cgcCTTCGGCATCCCTGGCCAACACCTCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCGTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAg                                                                                                                        <  2:2240267/150‑1 (MQ=255)
                             ccTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTa                                                                                                                      <  2:2418398/150‑1 (MQ=255)
                              cTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTaa                                                                                                                     >  1:4483884/1‑150 (MQ=255)
                              cTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTaa                                                                                                                     <  1:117169/150‑1 (MQ=255)
                               ttCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAAt                                                                                                                    <  2:2966024/150‑1 (MQ=255)
                                tCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAAtt                                                                                                                   <  1:3626373/150‑1 (MQ=255)
                                   gCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGTGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAg                                                                                                                <  1:1622507/150‑1 (MQ=255)
                                     aTCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGATCGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGaa                                                                                                              >  2:3619919/1‑150 (MQ=255)
                                         aTGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGt                                                                                                          <  2:1433008/150‑1 (MQ=255)
                                         aTGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGt                                                                                                          >  1:2211653/1‑150 (MQ=255)
                                          tGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGtt                                                                                                         >  1:2640455/1‑150 (MQ=255)
                                          tGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGtt                                                                                                         <  2:2792455/150‑1 (MQ=255)
                                           ggCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGATCGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTg                                                                                                        <  2:3779747/150‑1 (MQ=255)
                                           ggCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTg                                                                                                        >  1:813109/1‑150 (MQ=255)
                                               aaCACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGACATGTTGgaga                                                                                                    >  2:3110366/1‑150 (MQ=255)
                                                   caTCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGATAAAtt                                                                                                <  2:831728/150‑1 (MQ=255)
                                                   caTCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGATAAAtt                                                                                                <  2:3922668/150‑1 (MQ=255)
                                                   caTCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGATAAAtt                                                                                                <  1:27092/150‑1 (MQ=255)
                                                   caTCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGATAAAtt                                                                                                <  1:26929/150‑1 (MQ=255)
                                                     tCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGATCGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTAt                                                                                              <  2:191975/150‑1 (MQ=255)
                                                          cGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCatga                                                                                         >  1:3618275/1‑150 (MQ=255)
                                                          cGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCatga                                                                                         >  1:1113355/1‑150 (MQ=255)
                                                               aGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCaa                                                                                    <  2:4170546/150‑1 (MQ=255)
                                                               aGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCaa                                                                                    <  1:3484909/150‑1 (MQ=255)
                                                                gACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAAc                                                                                   >  2:424205/1‑150 (MQ=255)
                                                                 aCCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGATAAATTATCATGATGCAACa                                                                                  >  2:1281787/1‑150 (MQ=255)
                                                                  ccAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGATAAATTATCATGATGCAACAt                                                                                 >  2:4099926/1‑150 (MQ=255)
                                                                     ggTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAg                                                                              >  1:2581381/1‑150 (MQ=255)
                                                                       ttGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGt                                                                            <  2:4580681/150‑1 (MQ=255)
                                                                        tGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTc                                                                           >  1:1245838/1‑150 (MQ=255)
                                                                          aaGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCaa                                                                         <  1:869844/150‑1 (MQ=255)
                                                                          aaGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCaa                                                                         >  1:500369/1‑150 (MQ=255)
                                                                           aGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGATCGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAAt                                                                        >  1:84920/1‑150 (MQ=255)
                                                                           aGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAAt                                                                        <  2:1887182/150‑1 (MQ=255)
                                                                            gCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGATCGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATg                                                                       <  1:1486136/150‑1 (MQ=255)
                                                                            gCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATg                                                                       <  1:1948133/150‑1 (MQ=255)
                                                                             cGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGATCGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGt                                                                      <  2:3510957/150‑1 (MQ=255)
                                                                                     gACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTc                                                              >  2:1679321/1‑150 (MQ=255)
                                                                                     gACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTc                                                              <  2:1488421/150‑1 (MQ=255)
                                                                                     gACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTc                                                              >  1:375774/1‑150 (MQ=255)
                                                                                      acacCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCg                                                             >  1:1723978/1‑150 (MQ=255)
                                                                                      acacCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCg                                                             >  1:1602180/1‑150 (MQ=255)
                                                                                          cATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTc                                                         >  1:2130859/1‑150 (MQ=255)
                                                                                          cATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTc                                                         >  1:4388631/1‑150 (MQ=255)
                                                                                             gCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAAt                                                      <  2:2138330/150‑1 (MQ=255)
                                                                                             gCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAAt                                                      <  2:10657/150‑1 (MQ=255)
                                                                                             gCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTACAAt                                                      >  2:3355464/1‑150 (MQ=255)
                                                                                                gAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCa                                                   >  1:4035783/1‑150 (MQ=255)
                                                                                                       gATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGATCGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCt                                            >  2:3806041/1‑150 (MQ=255)
                                                                                                        aTGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGATCGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCtt                                           <  2:444250/150‑1 (MQ=255)
                                                                                                         tGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGATCGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTa                                          >  2:4515448/1‑150 (MQ=255)
                                                                                                          gggCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAg                                         >  2:3898717/1‑150 (MQ=255)
                                                                                                          gggCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAg                                         >  1:695974/1‑150 (MQ=255)
                                                                                                          gggCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAg                                         <  2:3362972/150‑1 (MQ=255)
                                                                                                            gCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGaa                                       >  1:209801/1‑150 (MQ=255)
                                                                                                             ccATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGATAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAAc                                      >  1:415979/1‑150 (MQ=255)
                                                                                                              cATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACg                                     <  2:3232278/150‑1 (MQ=255)
                                                                                                               aTACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACgt                                    >  1:182257/1‑150 (MQ=255)
                                                                                                               aTACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACgt                                    >  1:2498197/1‑150 (MQ=255)
                                                                                                                tACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACgtg                                   >  1:898375/1‑150 (MQ=255)
                                                                                                                 aCCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACgtgt                                  <  1:1062502/150‑1 (MQ=255)
                                                                                                                     gCTTCATGTCTCAATCGACGAACTTGAGATCGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTa                              >  2:2692556/1‑150 (MQ=255)
                                                                                                                          aTGTCTCAATCGACGAACTTGAGATCGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCgt                         <  1:1440672/150‑1 (MQ=255)
                                                                                                                          aTGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCgt                         >  2:6862/1‑150 (MQ=255)
                                                                                                                          aTGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCgt                         <  2:2610315/150‑1 (MQ=255)
                                                                                                                          aTGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCgt                         >  1:4560581/1‑150 (MQ=255)
                                                                                                                          aTGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCgt                         >  2:1144676/1‑150 (MQ=255)
                                                                                                                           tGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGGACGTGTTTTACGCgtg                        >  2:2980721/1‑150 (MQ=255)
                                                                                                                           tGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCgtg                        <  2:1611700/150‑1 (MQ=255)
                                                                                                                            gTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCgtgg                       >  1:2853589/1‑150 (MQ=255)
                                                                                                                               tcAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGc                    >  2:1016635/1‑150 (MQ=255)
                                                                                                                                cAATCGACGAACTTGAGATCGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCg                   <  1:2565866/150‑1 (MQ=255)
                                                                                                                                cAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCg                   <  1:3579238/150‑1 (MQ=255)
                                                                                                                                cAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCg                   <  1:4066722/150‑1 (MQ=255)
                                                                                                                                   tCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCa                <  2:4016026/150‑1 (MQ=255)
                                                                                                                                    cgacgaACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCAt               >  1:4608865/1‑150 (MQ=255)
                                                                                                                                     gacgaACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATc              <  1:2913484/150‑1 (MQ=255)
                                                                                                                                       cgaACTTGAGATCGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGt            <  1:3619919/150‑1 (MQ=255)
                                                                                                                                       cgaACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGt            >  1:2350478/1‑150 (MQ=255)
                                                                                                                                        gaACTTGAGATCGTCTGGGAGCTGGTGCCGCCTTTCGCCAGTAATTCAGAAATGTTGTTGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTAAATCATGAAACCTTAGATCGTGTTTTACGCGTGGTGCGTAATAGTg           <  2:1559915/150‑1 (MQ=255)
                                                                                                                                        gaACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTg           <  1:1007428/150‑1 (MQ=255)
                                                                                                                                         aaCTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTgg          >  2:4538497/1‑150 (MQ=255)
                                                                                                                                           cTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGtt        <  2:1638230/150‑1 (MQ=255)
                                                                                                                                             tGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTc      <  1:275658/150‑1 (MQ=255)
                                                                                                                                                gaACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCAc   <  1:1344028/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGTTTCCACG  >  CP009273/3945357‑3945651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A