Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,168,773 A→T 5.3% A133A (GCA→GCT)  rplK → 50S ribosomal subunit protein L11

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,168,7730AT 5.3% 913.1 / 28.3 274A133A (GCA→GCT) rplK50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand):  ref base A (113/146);  new base T (8/7);  total (121/153)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

GCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGT  >  CP009273/4168624‑4168922
                                                                                                                                                     |                                                                                                                                                     
     tATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTc                                                                                                                                                  <  2:157395/150‑1 (MQ=255)
       tCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCa                                                                                                                                                <  2:4257188/150‑1 (MQ=255)
         aaGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCTCGTTCCATg                                                                                                                                              >  1:2895598/1‑150 (MQ=255)
           gTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATggg                                                                                                                                            >  2:783248/1‑150 (MQ=255)
           gTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATggg                                                                                                                                            <  1:1356040/150‑1 (MQ=255)
              tGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCt                                                                                                                                         >  1:2273692/1‑150 (MQ=255)
                gTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTgg                                                                                                                                       >  2:4462405/1‑150 (MQ=255)
                gTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTgg                                                                                                                                       >  1:2573110/1‑150 (MQ=255)
                  tCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTa                                                                                                                                     >  2:3647446/1‑150 (MQ=255)
                  tCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTa                                                                                                                                     <  1:2223838/150‑1 (MQ=255)
                    cGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGt                                                                                                                                   <  2:3565607/150‑1 (MQ=255)
                     ggTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTg                                                                                                                                  >  1:3503973/1‑150 (MQ=255)
                     ggTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTg                                                                                                                                  <  1:4295475/150‑1 (MQ=255)
                       tAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTgga                                                                                                                                >  1:262487/1‑150 (MQ=255)
                        aaGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTggag                                                                                                                               >  2:102306/1‑150 (MQ=255)
                        aaGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTggag                                                                                                                               >  1:2098774/1‑150 (MQ=255)
                          gCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTggagga                                                                                                                             <  2:3630588/150‑1 (MQ=255)
                          gCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTggagga                                                                                                                             <  1:1134652/150‑1 (MQ=255)
                           ccGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGAc                                                                                                                            <  1:2422380/150‑1 (MQ=255)
                           ccGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGAc                                                                                                                            <  2:3954799/150‑1 (MQ=255)
                                 aaagacaaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGaa                                                                                                                      >  1:3327885/1‑150 (MQ=255)
                                  aagacaaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGaaa                                                                                                                     <  1:1333610/150‑1 (MQ=255)
                                   agacaaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAAt                                                                                                                    <  2:1305000/150‑1 (MQ=255)
                                   agacaaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAAt                                                                                                                    <  1:450968/150‑1 (MQ=255)
                                    gacaaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATg                                                                                                                   >  1:3163495/1‑150 (MQ=255)
                                     acaaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATgg                                                                                                                  >  2:2959052/1‑150 (MQ=255)
                                     acaaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATgg                                                                                                                  >  2:2778831/1‑150 (MQ=255)
                                     acaaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATgg                                                                                                                  >  2:2130060/1‑150 (MQ=255)
                                     acaaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATgg                                                                                                                  <  1:1895125/150‑1 (MQ=255)
                                        aagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTa                                                                                                               <  1:1062721/150‑1 (MQ=255)
                                         agTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAATCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTaa                                                                                                              >  1:3082150/1‑150 (MQ=255)
                                            gggTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACt                                                                                                           <  2:802491/150‑1 (MQ=255)
                                            gggTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACt                                                                                                           >  2:4532083/1‑150 (MQ=255)
                                             ggTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTg                                                                                                          >  2:48920/1‑150 (MQ=255)
                                               tAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGAc                                                                                                        <  2:694562/150‑1 (MQ=255)
                                                    tttCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAgc                                                                                                   >  2:709852/1‑150 (MQ=255)
                                                     ttCCCGCGCTCAGCTGCAGGAAATCGCGCTGACCAAAGCTGCCGACATGACTGTTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAgcg                                                                                                  <  2:3191187/150‑1 (MQ=255)
                                                        ccGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCAt                                                                                               <  2:2528012/150‑1 (MQ=255)
                                                         cgcgCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATg                                                                                              >  1:4051154/1‑150 (MQ=255)
                                                           cgcTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCg                                                                                            <  2:1917118/150‑1 (MQ=255)
                                                            gcTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCgt                                                                                           >  2:3209548/1‑150 (MQ=255)
                                                            gcTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCgt                                                                                           <  1:1733478/150‑1 (MQ=255)
                                                             cTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCgtg                                                                                          <  2:2475968/150‑1 (MQ=255)
                                                              tCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCgtgt                                                                                         >  1:3206224/1‑150 (MQ=255)
                                                              tCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCgtgt                                                                                         >  1:3742128/1‑150 (MQ=255)
                                                               cAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGtt                                                                                        <  2:268632/150‑1 (MQ=255)
                                                               cAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGtt                                                                                        >  1:659755/1‑150 (MQ=255)
                                                               cAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGtt                                                                                        <  2:336121/150‑1 (MQ=255)
                                                                aGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTa                                                                                       <  1:560867/150‑1 (MQ=255)
                                                                 gCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTAt                                                                                      <  2:1162432/150‑1 (MQ=255)
                                                                          aaTCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGaa                                                                             <  1:3855702/150‑1 (MQ=255)
                                                                          aaTCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGaa                                                                             <  1:4232270/150‑1 (MQ=255)
                                                                           aTCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGaaa                                                                            >  2:3124065/1‑150 (MQ=255)
                                                                           aTCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGaaa                                                                            >  1:3959709/1‑150 (MQ=255)
                                                                             cgcgCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGt                                                                          >  1:1251981/1‑150 (MQ=255)
                                                                             cgcgCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGt                                                                          >  1:4077763/1‑150 (MQ=255)
                                                                              gcgcAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGtt                                                                         <  2:3816850/150‑1 (MQ=255)
                                                                               cgcAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTg                                                                        >  2:4229720/1‑150 (MQ=255)
                                                                               cgcAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTg                                                                        >  1:1586695/1‑150 (MQ=255)
                                                                                 cAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGAt                                                                      >  1:3365412/1‑150 (MQ=255)
                                                                                     ccAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCaa                                                                  <  1:3791223/150‑1 (MQ=255)
                                                                                      cAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAAc                                                                 >  2:2235698/1‑150 (MQ=255)
                                                                                      cAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAAc                                                                 >  1:3839641/1‑150 (MQ=255)
                                                                                       aaaGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAAcc                                                                <  1:592357/150‑1 (MQ=255)
                                                                                       aaaGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAAcc                                                                <  2:4614925/150‑1 (MQ=255)
                                                                                       aaaGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAAcc                                                                <  2:815750/150‑1 (MQ=255)
                                                                                          gCTGCCGACATGACTGGTGCCGAGATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCaaa                                                             <  2:1394230/150‑1 (MQ=255)
                                                                                            tGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCTCGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACa                                                           <  1:3115363/150‑1 (MQ=255)
                                                                                                gACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGTGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTAc                                                       >  2:1662730/1‑150 (MQ=255)
                                                                                                 aCATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACg                                                      >  2:4057539/1‑150 (MQ=255)
                                                                                                  cATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGa                                                     >  2:4075441/1‑150 (MQ=255)
                                                                                                      aCTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATc                                                 <  1:1041042/150‑1 (MQ=255)
                                                                                                      aCTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATc                                                 <  1:59033/150‑1 (MQ=255)
                                                                                                       cTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCa                                                <  1:2394420/150‑1 (MQ=255)
                                                                                                       cTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCa                                                <  1:2394548/150‑1 (MQ=255)
                                                                                                       cTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCa                                                <  1:3454561/150‑1 (MQ=255)
                                                                                                       cTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCa                                                <  2:3496867/150‑1 (MQ=255)
                                                                                                         ggTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAAc                                              <  2:959151/150‑1 (MQ=255)
                                                                                                         ggTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAAc                                              <  2:3213270/150‑1 (MQ=255)
                                                                                                         ggTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAAc                                              >  2:1831621/1‑150 (MQ=255)
                                                                                                          gTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACg                                             <  1:3610403/150‑1 (MQ=255)
                                                                                                          gTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACg                                             >  2:4474422/1‑150 (MQ=255)
                                                                                                           tGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGa                                            >  1:2922029/1‑150 (MQ=255)
                                                                                                            gCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGaa                                           >  2:3740140/1‑150 (MQ=255)
                                                                                                              cGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGc                                         <  1:858082/150‑1 (MQ=255)
                                                                                                                           aTGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCActgctg                            >  1:272993/1‑150 (MQ=255)
                                                                                                                           aTGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCACATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCActgctg                            <  1:1554296/150‑1 (MQ=255)
                                                                                                                            tGACTCGCTCCATCGAAGGTACTGCTCGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGa                           >  2:1107752/1‑150 (MQ=255)
                                                                                                                            tGACTCGCTCCATCGAAGGTACTGCTCGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGa                           >  2:2058807/1‑150 (MQ=255)
                                                                                                                            tGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGa                           <  2:2997917/150‑1 (MQ=255)
                                                                                                                            tGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGa                           <  2:3327885/150‑1 (MQ=255)
                                                                                                                             gACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGaa                          >  1:484455/1‑150 (MQ=255)
                                                                                                                               cTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAag                        <  2:3673259/150‑1 (MQ=255)
                                                                                                                                  gCTCCATCGAAGGTACTGCACGTTCCATGGGACNGGTAGTGGANGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGc                     <  1:2871200/150‑1 (MQ=255)
                                                                                                                                    tCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTg                   <  1:1683246/150‑1 (MQ=255)
                                                                                                                                      cATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGc                 <  2:2273692/150‑1 (MQ=255)
                                                                                                                                       aTCGAAGGTACTGCTCGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCg                <  2:1474305/150‑1 (MQ=255)
                                                                                                                                                   gCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGATCTGGCGACTGCTAAATTc    <  2:437048/150‑1 (MQ=255)
                                                                                                                                                   gCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTc    >  2:1920742/1‑150 (MQ=255)
                                                                                                                                                     aCGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGt  <  1:4405915/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGT  >  CP009273/4168624‑4168922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A