Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,208,975 C→A 23.0% S151Y (TCT→TAT)  aceK → isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,208,9750CA23.0% 707.4 / 153.0 282S151Y (TCT→TAT) aceKisocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base C (110/106);  new base A (43/23);  total (153/129)
Fisher's exact test for biased strand distribution p-value = 4.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 294 total aligned reads displayed.

CTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGT  >  CP009273/4208826‑4209124
                                                                                                                                                     |                                                                                                                                                     
cTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATATTTAGCTCTCAGCCAGATCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGACtc                                                                                                                                                       >  2:3674313/1‑150 (MQ=255)
  tttttAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAAtctc                                                                                                                                                     <  2:575416/150‑1 (MQ=255)
     ttAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTAc                                                                                                                                                  >  2:3083309/1‑150 (MQ=255)
       aaCTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCGTTCGTACCAATCCCCGCCCGCTGGCGAAAGACATTCACCCCGATCACGGCTGGGAATCTCTAcag                                                                                                                                                >  1:3294214/1‑148 (MQ=255)
       aaCTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATATTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAATACCCGCCCGCTGGAGAAAGACGTTCACCCCCATCACGGCTGGGAATATCAACTg                                                                                                                                                >  1:2773499/1‑150 (MQ=255)
        aCTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAGTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGa                                                                                                                                               <  1:3566640/150‑1 (MQ=255)
            cGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATgcg                                                                                                                                           >  1:4204409/1‑150 (MQ=255)
            cGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATgcg                                                                                                                                           >  2:3801879/1‑150 (MQ=255)
            cGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACAGATgcg                                                                                                                                           >  2:1475777/1‑150 (MQ=255)
             gtttACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATATTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTACCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATgcgc                                                                                                                                          >  1:1486148/4‑150 (MQ=255)
                tACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGtt                                                                                                                                       >  2:3633914/1‑150 (MQ=255)
                    gTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCa                                                                                                                                   <  2:2621409/150‑1 (MQ=255)
                     tCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAg                                                                                                                                  <  2:439521/150‑1 (MQ=255)
                      cGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGc                                                                                                                                 >  2:1366010/1‑150 (MQ=255)
                       ggTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCg                                                                                                                                <  2:3717411/150‑1 (MQ=255)
                         ttATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGAc                                                                                                                              <  1:1544746/150‑1 (MQ=255)
                         ttATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGAc                                                                                                                              >  2:2631362/1‑150 (MQ=255)
                         ttATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGAc                                                                                                                              <  2:3171093/150‑1 (MQ=255)
                          tATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTAGTACCATTCCCCGCCCCCGGGAGAAAGACTTTCACCCCCATCACGGCTGGGAATATCTACTGATTCGCGTTATCAGCGAcc                                                                                                                             >  1:1656033/1‑150 (MQ=255)
                           aTTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCt                                                                                                                            >  2:623887/1‑150 (MQ=255)
                               gACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCg                                                                                                                        <  2:880655/150‑1 (MQ=255)
                                 ccaccGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGct                                                                                                                      <  1:2305047/150‑1 (MQ=255)
                                       ctcgctTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCCGAGCGCCGCTTTCGTAACATTCCCCGCCCGCTGGAGAAAGACTTTCAACCCGATCACGGCTGGGAATATCTACTGATGAGCGTTATCAGCGAACTACCCctgcgccg                                                                                                                >  2:1660928/1‑149 (MQ=255)
                                       ctcgctTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGctgcgcct                                                                                                                <  2:1528955/150‑1 (MQ=255)
                                       ctcgctTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGctgcgcct                                                                                                                <  2:1210083/150‑1 (MQ=255)
                                        tcgctTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGctgcgcctg                                                                                                               >  2:4186419/1‑150 (MQ=255)
                                          gctTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGctgcgcctgcg                                                                                                             >  1:1110496/1‑150 (MQ=255)
                                          gctTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGctgcgcctgcg                                                                                                             >  2:2162752/1‑150 (MQ=255)
                                            ttACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCt                                                                                                           <  1:3418596/150‑1 (MQ=255)
                                             tACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTg                                                                                                          <  1:2682811/150‑1 (MQ=255)
                                             tACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAATACCCGCCCGCTGGAGAAAGACTTTCACCCCCATCACGGCGGGGAATCTCTACTGATGCGCGTTATAAGCGACCTACCGcagcgcctgcgcgg                                                                                                          >  1:1266813/1‑148 (MQ=255)
                                               cTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGc                                                                                                        <  2:2862892/150‑1 (MQ=255)
                                               cTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTAACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCAGCGCCTGCGCTGGc                                                                                                        >  1:1055916/1‑150 (MQ=255)
                                                tCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCa                                                                                                       >  2:4607999/1‑150 (MQ=255)
                                                tCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCa                                                                                                       <  1:2079343/150‑1 (MQ=255)
                                                  ccGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGa                                                                                                     >  1:2522160/1‑150 (MQ=255)
                                                   cGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGaa                                                                                                    >  2:3300082/1‑150 (MQ=255)
                                                    gAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAAt                                                                                                   <  1:615052/150‑1 (MQ=255)
                                                    gAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAAt                                                                                                   >  1:3609051/1‑150 (MQ=255)
                                                    gAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAAt                                                                                                   >  1:4464796/1‑150 (MQ=255)
                                                     aGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATa                                                                                                  >  1:1580515/1‑150 (MQ=255)
                                                       cGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATaaa                                                                                                <  2:2815033/150‑1 (MQ=255)
                                                       cGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATaaa                                                                                                <  1:3786928/150‑1 (MQ=255)
                                                        ggCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATaaaa                                                                                               >  1:127186/1‑150 (MQ=255)
                                                        ggCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATaaaa                                                                                               >  1:3965161/1‑150 (MQ=255)
                                                          cTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGc                                                                                             >  1:4453985/1‑150 (MQ=255)
                                                          cTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGc                                                                                             >  1:281304/1‑150 (MQ=255)
                                                          cTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGc                                                                                             <  1:3508206/150‑1 (MQ=255)
                                                           tttttATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGcc                                                                                            >  1:1845279/1‑150 (MQ=255)
                                                           tttttATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGcc                                                                                            >  1:560745/1‑150 (MQ=255)
                                                             tttATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCAGt                                                                                          <  2:2560290/150‑1 (MQ=255)
                                                              ttATCTTGAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTg                                                                                         <  2:3339008/150‑1 (MQ=255)
                                                               tATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGa                                                                                        >  1:3700081/1‑150 (MQ=255)
                                                                aTCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGAc                                                                                       <  2:1308703/150‑1 (MQ=255)
                                                                        ctctCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCcatta                                                                               >  1:1791158/1‑150 (MQ=255)
                                                                         tctcAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCcattac                                                                              >  1:1424500/1‑150 (MQ=255)
                                                                          ctcAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCcattaca                                                                             >  1:829600/1‑150 (MQ=255)
                                                                             aGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCcattacatta                                                                          <  1:2203146/150‑1 (MQ=255)
                                                                                 agagCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCg                                                                      <  1:3874726/150‑1 (MQ=255)
                                                                                 agagCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCTCGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATATAAGCCGTGACATCCATTACATTATTCg                                                                      <  2:3291504/150‑1 (MQ=255)
                                                                                 agagCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCg                                                                      >  2:395749/1‑150 (MQ=255)
                                                                                      gccgcTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATc                                                                 >  2:2173251/1‑150 (MQ=255)
                                                                                      gccgcTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATc                                                                 >  2:3115124/1‑150 (MQ=255)
                                                                                       ccgcTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCt                                                                >  2:14544/1‑150 (MQ=255)
                                                                                        cgcTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTg                                                               <  1:392359/150‑1 (MQ=255)
                                                                                           tttCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACg                                                            >  2:797999/1‑150 (MQ=255)
                                                                                            ttCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACgg                                                           >  2:410655/1‑150 (MQ=255)
                                                                                            ttCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACgg                                                           >  2:4146238/1‑150 (MQ=255)
                                                                                                 aCCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGTCTGTTAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAAct                                                      <  2:1681808/150‑2 (MQ=255)
                                                                                                   cATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCt                                                    <  1:1316991/150‑1 (MQ=255)
                                                                                                     ttCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTgg                                                  <  1:4043919/150‑1 (MQ=255)
                                                                                                        cccgcccgCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGga                                               <  1:3037982/150‑1 (MQ=255)
                                                                                                              cgcTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGgacagaca                                         <  1:1349815/150‑1 (MQ=255)
                                                                                                                cTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAAc                                       >  1:1885884/1‑150 (MQ=255)
                                                                                                                cTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAAc                                       >  2:1039428/1‑150 (MQ=255)
                                                                                                                   gagAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTc                                    >  1:2022654/3‑150 (MQ=255)
                                                                                                                    agAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTcg                                   >  2:733376/2‑150 (MQ=255)
                                                                                                                    agAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTcg                                   >  2:3620757/2‑150 (MQ=255)
                                                                                                                    agAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTcg                                   <  1:736684/149‑1 (MQ=255)
                                                                                                                    agAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTcg                                   <  1:2956112/149‑1 (MQ=255)
                                                                                                                       aaGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCgg                                >  1:4176528/1‑150 (MQ=255)
                                                                                                                       aaGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCgg                                >  1:461307/1‑150 (MQ=255)
                                                                                                                              tCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCa                         <  2:2522160/150‑1 (MQ=255)
                                                                                                                               cACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCAt                        <  1:2162752/150‑1 (MQ=255)
                                                                                                                                 ccccGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCAttt                      <  2:4070897/150‑1 (MQ=255)
                                                                                                                                 ccccGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCAttt                      >  1:3493024/1‑150 (MQ=255)
                                                                                                                                 ccccGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCAttt                      >  1:3194837/1‑150 (MQ=255)
                                                                                                                                  cccGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTa                     >  1:183603/1‑150 (MQ=255)
                                                                                                                                  cccGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTa                     <  2:4253387/150‑1 (MQ=255)
                                                                                                                                   ccGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTAc                    <  2:1764382/150‑1 (MQ=255)
                                                                                                                                      aTCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAgg                 <  2:2101883/150‑1 (MQ=255)
                                                                                                                                       tCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACGGACAACCTCGCGGAAAGTCATTTACAGGt                >  1:936999/1‑150 (MQ=255)
                                                                                                                                        cACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTg               <  1:1475777/150‑1 (MQ=255)
                                                                                                                                        cACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTg               <  1:2855070/150‑1 (MQ=255)
                                                                                                                                         aCGGCTTGGAATATCTACTGATGAGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTAGTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTgg              <  2:3512864/150‑1 (MQ=255)
                                                                                                                                          cGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGc             <  2:1580499/150‑1 (MQ=255)
                                                                                                                                              tGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGcgaac         >  1:422354/1‑150 (MQ=255)
                                                                                                                                                ggAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGcgaacga       >  2:1850997/1‑150 (MQ=255)
                                                                                                                                                  aaTCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCgaacgaac     <  2:2975007/150‑1 (MQ=255)
                                                                                                                                                    tctcTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTg   <  2:1943932/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGT  >  CP009273/4208826‑4209124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A