Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F54 I0 R1 640 319.5 10493376 95.3% 10000187 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,090,918 T→A 22.2% intergenic (‑25/‑43) insE1 ← / → ymdE IS3 transposase A/pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,090,9180TA22.2% 534.1 / 155.6 238intergenic (‑25/‑43)insE1/ymdEIS3 transposase A/pseudogene
Reads supporting (aligned to +/- strand):  ref base T (100/85);  new base A (32/21);  total (132/106)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

TCAGCAAGCTTCAGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGTAACAGC  >  CP009273/1090811‑1091067
                                                                                                           |                                                                                                                                                     
tCAGCAAGCTTCAGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGt                                                                                                             <  2:1234451/150‑1 (MQ=11)
   gCAAGCTTCAGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGcc                                                                                                          >  2:4868000/1‑150 (MQ=32)
   gCAAGCTTCAGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGcc                                                                                                          <  1:2919113/150‑1 (MQ=32)
       gCTTCAGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGc                                                                                                      >  1:1009545/1‑150 (MQ=25)
        cTTCAGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGca                                                                                                     >  1:3237383/1‑150 (MQ=37)
         ttCAGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGcac                                                                                                    >  1:4920398/1‑150 (MQ=38)
          tCAGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGcaca                                                                                                   >  2:4515747/1‑150 (MQ=38)
           cAGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTGTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCacac                                                                                                  <  2:4701726/150‑1 (MQ=255)
            aGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACAcg                                                                                                 >  2:171181/1‑150 (MQ=40)
             gggCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACAcgc                                                                                                >  1:3323309/1‑150 (MQ=40)
                cTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGtt                                                                                             >  1:3985235/1‑150 (MQ=255)
                 ttCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGttt                                                                                            >  1:873016/1‑150 (MQ=255)
                  tCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTg                                                                                           <  1:1916930/150‑1 (MQ=255)
                         gAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAg                                                                                    >  2:4282763/1‑150 (MQ=255)
                          aaaTTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGa                                                                                   <  2:1573941/150‑1 (MQ=255)
                            aTTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACg                                                                                 <  2:1043619/150‑1 (MQ=255)
                            aTTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACg                                                                                 <  2:18009/150‑1 (MQ=255)
                             ttCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACgg                                                                                >  2:268047/1‑150 (MQ=255)
                             ttCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACgg                                                                                <  1:4715415/150‑1 (MQ=255)
                               cAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCg                                                                              <  1:4120276/150‑1 (MQ=255)
                                 ggCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGtt                                                                            <  2:3554818/150‑1 (MQ=255)
                                  gCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTc                                                                           <  1:1401846/150‑1 (MQ=255)
                                  gCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTc                                                                           <  2:1588709/150‑1 (MQ=255)
                                   cGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCa                                                                          <  1:1137942/150‑1 (MQ=255)
                                   cGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCa                                                                          <  1:1218729/150‑1 (MQ=255)
                                    gAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAg                                                                         <  2:3993613/150‑1 (MQ=255)
                                    gAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAg                                                                         <  2:409743/150‑1 (MQ=255)
                                       tGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCTCTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCa                                                                      <  1:2171489/150‑1 (MQ=255)
                                       tGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCa                                                                      >  2:2409076/1‑150 (MQ=255)
                                       tGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGACGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCa                                                                      >  2:4951078/1‑150 (MQ=255)
                                         cTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCact                                                                    >  2:4014371/1‑148 (MQ=255)
                                         cTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAAt                                                                    <  2:558905/150‑1 (MQ=255)
                                          tGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATg                                                                   <  1:5228699/150‑1 (MQ=255)
                                          tGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATg                                                                   >  2:1362953/1‑150 (MQ=255)
                                           gTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATgc                                                                  >  2:1596753/1‑150 (MQ=255)
                                           gTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATgc                                                                  >  1:77987/1‑150 (MQ=255)
                                              tACGGGGTTTTTTACTTGTTGATACTGTTTTTTTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCAGCGTGTACACTATTGCTGGGTAAGATCAGTGACAGGAACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGcgcg                                                               >  2:4423546/1‑150 (MQ=18)
                                              tACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGcgcg                                                               >  2:3807386/1‑150 (MQ=255)
                                              tACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGcgcg                                                               >  1:2259525/1‑150 (MQ=255)
                                              tACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGcgcg                                                               >  2:113568/1‑150 (MQ=255)
                                               aCGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGa                                                              >  2:4808645/1‑150 (MQ=255)
                                                  gggTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTa                                                           <  2:5009081/150‑1 (MQ=255)
                                                  gggTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTa                                                           >  1:1448287/1‑150 (MQ=255)
                                                  gggTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTa                                                           >  2:630274/1‑150 (MQ=255)
                                                    gTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATc                                                         <  1:2532699/150‑1 (MQ=255)
                                                     ttttttACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCt                                                        >  2:2296209/1‑150 (MQ=255)
                                                      tttttACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCtt                                                       <  1:3535565/150‑1 (MQ=255)
                                                      tttttACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCtt                                                       >  2:2882570/1‑150 (MQ=255)
                                                       ttttACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTg                                                      <  1:4888609/150‑1 (MQ=255)
                                                        tttACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGc                                                     >  2:2020270/1‑150 (MQ=255)
                                                        tttACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGc                                                     >  1:3843340/1‑150 (MQ=255)
                                                         ttACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGcc                                                    >  2:1112151/1‑150 (MQ=255)
                                                         ttACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGcc                                                    >  1:552208/1‑150 (MQ=255)
                                                          tACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCg                                                   <  2:2378782/150‑1 (MQ=255)
                                                          tACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCg                                                   >  1:473882/1‑150 (MQ=255)
                                                          tACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCg                                                   >  2:1382735/1‑150 (MQ=255)
                                                           aCTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCgg                                                  <  2:4602279/150‑1 (MQ=255)
                                                            cTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGa                                                 >  2:3642950/1‑150 (MQ=255)
                                                            cTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGa                                                 <  1:3067123/150‑1 (MQ=255)
                                                              ggTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACt                                               >  1:5211209/1‑150 (MQ=255)
                                                               gTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTg                                              >  2:222366/1‑150 (MQ=255)
                                                               gTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTg                                              >  1:1804404/1‑150 (MQ=255)
                                                                  gATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGat                                           >  2:11234/1‑150 (MQ=255)
                                                                  gATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGat                                           <  2:3884407/150‑1 (MQ=255)
                                                                    tACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGatat                                         <  1:2414697/150‑1 (MQ=255)
                                                                    tACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGatat                                         <  2:3475346/150‑1 (MQ=255)
                                                                    tACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGatat                                         <  2:1864449/150‑1 (MQ=255)
                                                                     aCTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATc                                        >  2:478090/1‑150 (MQ=255)
                                                                     aCTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATc                                        >  1:995404/1‑150 (MQ=255)
                                                                     aCTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATc                                        <  2:2855908/150‑1 (MQ=255)
                                                                     aCTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATc                                        >  1:4601770/1‑150 (MQ=255)
                                                                     aCTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATc                                        >  1:4379634/1‑150 (MQ=255)
                                                                     aCTGTTTTTGTAATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATc                                        <  2:924041/150‑1 (MQ=255)
                                                                      cTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCg                                       >  1:1311929/1‑150 (MQ=255)
                                                                       tGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGc                                      >  2:5225965/1‑150 (MQ=255)
                                                                        gTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCt                                     >  1:45220/1‑150 (MQ=255)
                                                                         tttttGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCtt                                    >  1:4251639/1‑150 (MQ=255)
                                                                         tttttGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCtt                                    <  2:3735899/150‑1 (MQ=255)
                                                                             tGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACCCGCGTTTGATGGACGACGGCGTGCAGGCAATGCGCGCTTAGCTTGCCGGACTGGAGATCGCTTCGcc                                >  2:2801181/1‑150 (MQ=25)
                                                                             tGTCATGTGAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGcc                                >  1:3728004/1‑150 (MQ=255)
                                                                               tCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCag                              <  1:171181/150‑1 (MQ=255)
                                                                                cATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCaga                             <  2:3037400/150‑1 (MQ=255)
                                                                                cATGTGAGACACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTACGCCaga                             >  2:1500306/1‑150 (MQ=255)
                                                                                    tgAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCAt                         <  1:3513140/150‑1 (MQ=255)
                                                                                    tgAGTCACCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCAt                         >  1:4832790/1‑150 (MQ=255)
                                                                                      aGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCa                       <  2:1196120/150‑1 (MQ=255)
                                                                                        tCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCTgg                     >  1:4866292/1‑150 (MQ=255)
                                                                                        tCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAgg                     >  2:1738943/1‑150 (MQ=255)
                                                                                          aCCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGtt                   <  1:59863/150‑1 (MQ=255)
                                                                                          aCCTCTGACTGAGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGtt                   >  1:3315636/1‑150 (MQ=255)
                                                                                                gactgaGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCtgatg             >  2:1360457/1‑150 (MQ=255)
                                                                                                gactgaGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCtgatg             <  1:5171564/150‑1 (MQ=255)
                                                                                                gactgaGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCtgatg             <  1:1044127/150‑1 (MQ=255)
                                                                                                gactgaGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCtgatg             <  1:642542/150‑1 (MQ=255)
                                                                                                gactgaGAGTTAACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCtgatg             <  1:4132221/150‑1 (MQ=255)
                                                                                                   tgaGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAAc          >  2:3796037/1‑150 (MQ=255)
                                                                                                     agagTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGt        >  2:4741370/1‑150 (MQ=255)
                                                                                                      gagTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCATTGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGTa       <  1:4670105/150‑1 (MQ=255)
                                                                                                        gTTTACTCACTTAGCCGCGTGTCCACTCTTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGTAAc     <  2:4240046/150‑1 (MQ=255)
                                                                                                          ttACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGTAACAg   <  2:3187109/150‑1 (MQ=255)
                                                                                                           |                                                                                                                                                     
TCAGCAAGCTTCAGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGTAACAGC  >  CP009273/1090811‑1091067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A