Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F54 I0 R1 640 319.5 10493376 95.3% 10000187 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,599,970 T→A 9.2% D221E (GAT→GAA)  lsrB → autoinducer 2‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,599,9700TA 9.2% 937.4 / 62.5 282D221E (GAT→GAA) lsrBautoinducer 2‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (129/126);  new base A (20/5);  total (150/132)
Fisher's exact test for biased strand distribution p-value = 5.67e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 284 total aligned reads displayed.

AAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCA  >  CP009273/1599821‑1600119
                                                                                                                                                     |                                                                                                                                                     
  agaagCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGc                                                                                                                                                     >  2:3047215/1‑150 (MQ=255)
  agaagCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGc                                                                                                                                                     <  2:1320242/150‑1 (MQ=255)
    aagcgaaagcgaAAATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCa                                                                                                                                                   >  1:1536900/1‑150 (MQ=255)
    aagcgaaagcgaAAATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGAAGCCa                                                                                                                                                   >  1:3712793/1‑150 (MQ=255)
        gaaagcgaaaATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGAAGCCAACGc                                                                                                                                               >  2:1569225/1‑150 (MQ=255)
          aagcgaaaATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGccc                                                                                                                                             <  2:2187092/150‑1 (MQ=255)
             cgaaaATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCTCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACTCCATTATCGCCCCCGATGCCAACTCCCTGc                                                                                                                                          >  2:1671606/1‑150 (MQ=255)
              gaaaATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGcc                                                                                                                                         >  2:4056232/1‑150 (MQ=255)
               aaaaTCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGccc                                                                                                                                        <  1:1135010/150‑1 (MQ=255)
               aaaaTCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGccc                                                                                                                                        <  1:4771928/150‑1 (MQ=255)
               aaaaTCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGccc                                                                                                                                        >  1:985938/1‑150 (MQ=255)
                  aTCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCt                                                                                                                                     <  1:2501488/150‑1 (MQ=255)
                    cGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGc                                                                                                                                   <  1:3776761/150‑1 (MQ=255)
                      ccAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCg                                                                                                                                 >  2:1594638/1‑150 (MQ=255)
                        aaaGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGca                                                                                                                               >  1:566167/1‑150 (MQ=255)
                          cgagCAGCCCGGCGGGGAAATTGTCACTACGCAGTTTGGCTATACCGAGGCCACTAACTCGGTACAACCCGCAGAAGGAATATTAACAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGcaca                                                                                                                             <  2:3276726/149‑1 (MQ=255)
                          agagCATCCATGCTGGGAAATTGTCTCTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGcaca                                                                                                                             <  2:2727208/150‑1 (MQ=255)
                          agagCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGcaca                                                                                                                             <  2:3821638/150‑1 (MQ=255)
                          agagCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGcaca                                                                                                                             >  1:3561978/1‑150 (MQ=255)
                            agCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAg                                                                                                                           >  2:1195534/1‑150 (MQ=255)
                            agCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAg                                                                                                                           >  1:4042460/1‑150 (MQ=255)
                              cATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGcc                                                                                                                         <  1:222602/150‑1 (MQ=255)
                              cATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGcc                                                                                                                         >  2:2908778/1‑150 (MQ=255)
                              cATCCAGGCTGGGAAATTGTCACTACGCAGATTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGcc                                                                                                                         <  2:5128421/150‑1 (MQ=255)
                                  cAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAg                                                                                                                     >  1:5052530/1‑150 (MQ=255)
                                  cAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAg                                                                                                                     <  2:4763929/150‑1 (MQ=255)
                                    ggCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGaa                                                                                                                   <  1:43503/150‑1 (MQ=255)
                                       tGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGAGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAAc                                                                                                                >  2:2976255/1‑150 (MQ=255)
                                        gggAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACt                                                                                                               >  2:4950206/1‑150 (MQ=255)
                                          gAAATTGTCACTACGCAGTTTTGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGAAGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTg                                                                                                             <  2:1824330/150‑1 (MQ=255)
                                          gAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTg                                                                                                             <  1:1328120/150‑1 (MQ=255)
                                          gAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTg                                                                                                             <  1:4725256/150‑1 (MQ=255)
                                           aaaTTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGa                                                                                                            <  1:3632962/150‑1 (MQ=255)
                                              ttGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGaaaa                                                                                                         <  1:459311/150‑1 (MQ=255)
                                              ttGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGaaaa                                                                                                         <  1:526546/150‑1 (MQ=255)
                                               tGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGaaaaa                                                                                                        >  1:3027287/1‑150 (MQ=255)
                                                gTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAAt                                                                                                       <  2:4465357/150‑1 (MQ=255)
                                                gTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAAt                                                                                                       <  2:807443/150‑1 (MQ=255)
                                                gTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAAt                                                                                                       >  1:2842921/1‑150 (MQ=255)
                                                gTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAAt                                                                                                       <  2:3209616/150‑1 (MQ=255)
                                                   aCTACGCAGTTTGGCTATAACGATGCCACTCAATCGGAACCAACCGCAGAAGGAATCGTCAAAGCGAATAGCGATCTAGACGCCATAATCGACCCCGACGCCACCGCCCAGACCGCTGCCGCACAAGCCGCAGAAAACAAGAAAAATGAc                                                                                                    >  2:3655895/1‑150 (MQ=255)
                                                   aCTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGAc                                                                                                    >  1:200974/1‑150 (MQ=255)
                                                    cTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACa                                                                                                   <  1:4462906/150‑1 (MQ=255)
                                                    cTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACa                                                                                                   <  2:1054552/150‑1 (MQ=255)
                                                      aCGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACaaa                                                                                                 <  1:3249767/150‑1 (MQ=255)
                                                          aGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAg                                                                                             >  1:3842040/1‑150 (MQ=255)
                                                          aGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAg                                                                                             <  2:4905289/150‑1 (MQ=255)
                                                          aGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGAAGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAg                                                                                             <  2:768479/150‑1 (MQ=255)
                                                           gTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGc                                                                                            <  1:3845756/150‑1 (MQ=255)
                                                            tttGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGct                                                                                           <  2:2625352/150‑2 (MQ=255)
                                                              tGGCTATAACGATGCCACTAAATCGTTACAAACAGCAGAATGAATATTAACAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCAGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGAt                                                                                         <  2:5199800/150‑1 (MQ=255)
                                                              tGGCCATAACGCTGCCAATAAATCGTTACAAACCGCATAAGTAAGATTAACAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACACAGTAGCGAt                                                                                         <  2:4290023/150‑1 (MQ=255)
                                                                gCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTg                                                                                       <  1:173077/150‑1 (MQ=255)
                                                                      aCGATGCCACTAACTCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGAt                                                                                 <  2:178059/150‑1 (MQ=255)
                                                                        gATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTc                                                                               >  1:1188938/1‑150 (MQ=255)
                                                                           cccACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGt                                                                            <  2:4682949/149‑1 (MQ=255)
                                                                            ccACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTa                                                                           <  2:3503243/150‑1 (MQ=255)
                                                                             cACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTAc                                                                          <  2:915942/150‑1 (MQ=255)
                                                                             cACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTAc                                                                          >  1:4687364/1‑150 (MQ=255)
                                                                                   aTCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAATACGCCaaa                                                                    >  2:2882649/1‑150 (MQ=255)
                                                                                     cGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAAtg                                                                  >  1:679848/1‑150 (MQ=255)
                                                                                      gTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAAtgt                                                                 <  1:3741208/150‑1 (MQ=255)
                                                                                       ttACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTAACGCCCCCGATGCCAACGCCCTGACCGCTGCCGCACAAGCCGCATAAAACTTGAAAAATAACAAAGTAGCGAATGACGGATTCAGTACGCCAAAtgag                                                                >  2:527899/1‑148 (MQ=255)
                                                                                         aCAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGAt                                                              >  1:4170748/1‑150 (MQ=255)
                                                                                           aaaCCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATgc                                                            >  1:621505/1‑150 (MQ=255)
                                                                                                 cAGAAGGACTATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCCAATGTGATGCGCCCGt                                                      >  2:3549031/1‑150 (MQ=255)
                                                                                                 cAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGt                                                      >  1:4193991/1‑150 (MQ=255)
                                                                                                  aGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTa                                                     >  1:4022615/1‑150 (MQ=255)
                                                                                                  aGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTa                                                     <  2:4392195/150‑1 (MQ=255)
                                                                                                       gAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTag                                                >  2:4932204/1‑150 (MQ=255)
                                                                                                       gAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTag                                                >  2:3278186/1‑150 (MQ=255)
                                                                                                         atatTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTagag                                              >  2:4138870/1‑150 (MQ=255)
                                                                                                          tatTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAgc                                             <  1:1635787/150‑1 (MQ=255)
                                                                                                           atTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGAATGTAGAgcg                                            >  2:4569932/1‑150 (MQ=255)
                                                                                                             tAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGcgcg                                          <  2:2851409/150‑1 (MQ=255)
                                                                                                                 aGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCAc                                      >  1:3528885/1‑150 (MQ=255)
                                                                                                                   cGTATAGCGATCTCGACGCCATTATCGCCCCCGAAGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACgg                                    >  1:1084925/1‑150 (MQ=255)
                                                                                                                    gTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGt                                   >  1:3007587/1‑150 (MQ=255)
                                                                                                                       taGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGaa                                <  2:64911/150‑1 (MQ=255)
                                                                                                                       taGCGATCTCGACGCCATTATCGCCCCCGAAGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGaa                                >  1:1612489/1‑150 (MQ=255)
                                                                                                                        aGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGaaa                               >  1:2578504/1‑150 (MQ=255)
                                                                                                                        aGCGATCTCAACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGaaa                               >  1:2666815/1‑150 (MQ=255)
                                                                                                                              ctcGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAAttt                         <  2:3626070/150‑1 (MQ=255)
                                                                                                                              ctcGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAAttt                         >  2:2719752/1‑150 (MQ=255)
                                                                                                                                cGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTgg                       >  1:78804/1‑150 (MQ=255)
                                                                                                                                cGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTgg                       >  2:4679152/1‑150 (MQ=255)
                                                                                                                                 gACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGc                      >  2:1480526/1‑150 (MQ=255)
                                                                                                                                  aCGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGcc                     <  1:2908778/150‑1 (MQ=255)
                                                                                                                                     ccATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGTTTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCtgt                  <  2:416953/150‑1 (MQ=255)
                                                                                                                                      cATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCtgtg                 <  1:4915967/150‑1 (MQ=255)
                                                                                                                                       aTTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAACGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTgg                <  1:4296486/150‑1 (MQ=255)
                                                                                                                                       aTTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTgg                <  1:3273418/150‑1 (MQ=255)
                                                                                                                                          aTCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGAt             <  2:188245/150‑1 (MQ=255)
                                                                                                                                             gCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGAtgtg          <  1:4692773/150‑1 (MQ=255)
                                                                                                                                               ccccGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGt        >  1:4480927/1‑150 (MQ=255)
                                                                                                                                                 ccGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTc      >  1:1498864/1‑150 (MQ=255)
                                                                                                                                                 ccGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTc      <  2:2115104/150‑1 (MQ=255)
                                                                                                                                                  cGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCa     >  2:2802/1‑150 (MQ=255)
                                                                                                                                                   gATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAg    <  2:377592/150‑1 (MQ=255)
                                                                                                                                                     tGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCa  <  2:241766/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCA  >  CP009273/1599821‑1600119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A