Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,099,378 A→C 40.9% intergenic (‑726/‑29) csgD ← / → csgB csgBAC operon transcriptional regulator/curlin nucleator protein, minor subunit in curli complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,099,3780AC40.9% 69.8 / inf 225intergenic (‑726/‑29)csgD/csgBcsgBAC operon transcriptional regulator/curlin nucleator protein, minor subunit in curli complex
Reads supporting (aligned to +/- strand):  ref base A (67/66);  new base C (33/59);  total (100/125)
Fisher's exact test for biased strand distribution p-value = 4.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

TTTAATACTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTGAGTAAGT  >  CP009273/1099230‑1099527
                                                                                                                                                    |                                                                                                                                                     
tttAATACTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTaa                                                                                                                                                      <  2:2912483/150‑1 (MQ=255)
   aaTACTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATg                                                                                                                                                   <  1:1352170/150‑1 (MQ=255)
      aCTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTAc                                                                                                                                                >  1:1387045/1‑150 (MQ=255)
        tttGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGa                                                                                                                                              <  1:3380573/150‑1 (MQ=255)
           ggTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCa                                                                                                                                           >  2:269268/1‑150 (MQ=255)
           ggTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCa                                                                                                                                           >  1:1433600/1‑150 (MQ=255)
           ggTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCACCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCa                                                                                                                                           >  1:3700682/1‑150 (MQ=255)
              aTGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGt                                                                                                                                        >  1:979168/1‑150 (MQ=255)
                 aaCTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCa                                                                                                                                     <  2:291696/150‑1 (MQ=255)
                   cTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAgg                                                                                                                                   <  1:4301089/150‑1 (MQ=255)
                   cTAAAAAAGAAAAATACAACGCGCGGGCGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAgg                                                                                                                                   <  1:2054921/150‑1 (MQ=255)
                     aaaaaaGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGt                                                                                                                                 >  2:1551200/1‑150 (MQ=255)
                       aaaaGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGa                                                                                                                               >  2:2930480/1‑150 (MQ=255)
                            aaaaaTACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGacaaca                                                                                                                          <  1:558772/150‑1 (MQ=255)
                            aaaaaTACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGacaaca                                                                                                                          >  1:2182510/1‑150 (MQ=255)
                             aaaaTACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACAt                                                                                                                         >  2:2253534/1‑150 (MQ=255)
                               aaTACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGa                                                                                                                       <  1:1048263/150‑1 (MQ=255)
                               aaTACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGa                                                                                                                       >  1:2467936/1‑150 (MQ=255)
                                 tACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCATGTTGAAATGATTTAATTTCTTATATGTACGACCAGGTCCAGGGTGACAACATGaaa                                                                                                                     <  1:3139125/150‑1 (MQ=255)
                                   cAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGaaaaa                                                                                                                   <  2:2967902/150‑1 (MQ=255)
                                    aaCGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAAc                                                                                                                  <  1:3561576/150‑1 (MQ=255)
                                      cgcgcgGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACaa                                                                                                                >  1:3794169/1‑150 (MQ=255)
                                      cgcgcgGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACaa                                                                                                                >  1:1158993/1‑150 (MQ=255)
                                       gcgcgGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACaaa                                                                                                               <  1:2808201/150‑1 (MQ=255)
                                       gcgcgGGTGAGTTATTAAAAATATTTCCGCAGACATACTGTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACaaa                                                                                                               <  1:2808299/150‑1 (MQ=255)
                                         gcgGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAAtt                                                                                                             <  1:3991727/150‑1 (MQ=255)
                                             gTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGtta                                                                                                         <  2:1410108/150‑1 (MQ=255)
                                               gAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGttatt                                                                                                       >  1:2394118/1‑150 (MQ=255)
                                               gAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGttatt                                                                                                       <  2:1907266/150‑1 (MQ=255)
                                                 gTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGttattta                                                                                                     <  2:3949226/150‑1 (MQ=255)
                                                      taAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTatgatg                                                                                                <  1:773746/150‑1 (MQ=255)
                                                      taAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTatgatg                                                                                                >  1:1602831/1‑150 (MQ=255)
                                                       aaaaaTATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGt                                                                                               >  2:2037681/1‑150 (MQ=255)
                                                         aaaTATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTa                                                                                             >  2:1550441/1‑150 (MQ=255)
                                                          aaTATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTaa                                                                                            >  1:2461908/1‑150 (MQ=255)
                                                           atatTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAAc                                                                                           <  2:367295/150‑1 (MQ=255)
                                                           atatTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAAc                                                                                           <  1:2999806/150‑1 (MQ=255)
                                                            tatTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTGATGATGTTAACa                                                                                          >  2:3093874/1‑150 (MQ=255)
                                                               ttCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATa                                                                                       <  1:4427201/150‑1 (MQ=255)
                                                                tCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATAc                                                                                      >  1:1853273/1‑150 (MQ=255)
                                                                tCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATAc                                                                                      >  1:3272492/1‑150 (MQ=255)
                                                                tCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATAc                                                                                      <  2:4034218/150‑1 (MQ=255)
                                                                 ccGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACt                                                                                     >  2:2597139/1‑150 (MQ=255)
                                                                    cAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTggg                                                                                  >  1:2478282/1‑150 (MQ=255)
                                                                     aGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACGGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGt                                                                                 <  1:3366453/150‑1 (MQ=255)
                                                                      gACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTg                                                                                <  1:1717503/150‑1 (MQ=255)
                                                                         aTACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTgcgc                                                                             >  2:1835969/1‑150 (MQ=255)
                                                                          tACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGcc                                                                            <  1:845564/150‑1 (MQ=255)
                                                                            cTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTg                                                                          >  2:4521044/1‑150 (MQ=255)
                                                                            cTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTg                                                                          <  2:2394930/150‑1 (MQ=255)
                                                                               tCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGa                                                                       <  1:2140451/150‑1 (MQ=255)
                                                                                ccATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAAAAATACTGGGTGCGCCTGGGAt                                                                      >  2:3044544/1‑150 (MQ=255)
                                                                                    cGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCa                                                                  <  2:2857602/150‑1 (MQ=255)
                                                                                     gTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAg                                                                 >  2:4450439/1‑150 (MQ=255)
                                                                                      tAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGc                                                                <  1:1428853/150‑1 (MQ=255)
                                                                                       aaCGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGcc                                                               >  2:1087785/1‑150 (MQ=255)
                                                                                         cGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCgc                                                             >  2:1959549/1‑150 (MQ=255)
                                                                                            aGCGTTAACAAAATACAGTTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCgcagc                                                          <  1:64191/150‑1 (MQ=255)
                                                                                               gTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAgg                                                       >  1:777143/1‑150 (MQ=255)
                                                                                               gTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAgg                                                       >  1:1454185/1‑150 (MQ=255)
                                                                                                ttAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGt                                                      <  1:1990185/150‑1 (MQ=255)
                                                                                                ttAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGt                                                      <  2:338987/150‑1 (MQ=255)
                                                                                                  aaCAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTa                                                    <  1:937837/150‑1 (MQ=255)
                                                                                                   aCAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTAt                                                   <  1:4169979/150‑1 (MQ=255)
                                                                                                   aCAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTAt                                                   >  1:3090455/1‑150 (MQ=255)
                                                                                                        aTACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTAAACAATACTGGGTGCGCCTGGAATTGCAGCCGCAGCAGGTTATGAttt                                              >  1:2244067/1‑150 (MQ=255)
                                                                                                         tACAGGTTGCTTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTa                                             <  2:2447840/150‑1 (MQ=255)
                                                                                                           cAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGc                                           >  2:3288558/1‑150 (MQ=255)
                                                                                                            aGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCt                                          <  1:2146958/150‑1 (MQ=255)
                                                                                                             ggTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTa                                         <  2:37541/150‑1 (MQ=255)
                                                                                                              gTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGAGTTAGCTaa                                        >  1:2865556/1‑150 (MQ=255)
                                                                                                                tGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTaagt                                      <  1:3824599/150‑3 (MQ=255)
                                                                                                                tGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAAtt                                      <  1:3824614/150‑1 (MQ=255)
                                                                                                                 gCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTc                                     >  2:1873235/1‑150 (MQ=255)
                                                                                                                 gCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTc                                     >  2:1875108/1‑150 (MQ=255)
                                                                                                                  cGTTAACAACCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCa                                    >  2:1727167/1‑150 (MQ=255)
                                                                                                                       acaacCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAata                               >  2:2270858/1‑150 (MQ=255)
                                                                                                                        caacCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAatat                              <  2:1049710/150‑1 (MQ=255)
                                                                                                                        caacCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAatat                              >  1:2370400/1‑150 (MQ=255)
                                                                                                                         aacCAAGTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAAtata                             <  1:3638058/150‑1 (MQ=255)
                                                                                                                            cAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATTATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACt                          <  1:1798858/150‑1 (MQ=255)
                                                                                                                            cAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACt                          <  1:3204907/150‑1 (MQ=255)
                                                                                                                               gTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTcg                       >  2:700845/1‑150 (MQ=255)
                                                                                                                               gTTGAAATGATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTcg                       >  2:2982990/1‑150 (MQ=255)
                                                                                                                                   aaaTGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGt                   >  2:4151465/1‑150 (MQ=255)
                                                                                                                                    aaTGATTTAATGTCTTAAATGTACGCCCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTa                  <  2:3892680/150‑1 (MQ=255)
                                                                                                                                     aTGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTaa                 <  1:2401521/150‑1 (MQ=255)
                                                                                                                                     aTGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTaa                 <  1:2115309/150‑1 (MQ=255)
                                                                                                                                     aTGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTaa                 <  2:2086966/150‑1 (MQ=255)
                                                                                                                                       gATTTAATTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCCGAATATAACTTCGCGGTAAAt               <  1:3686861/150‑1 (MQ=255)
                                                                                                                                       gATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAAt               >  1:2848248/1‑150 (MQ=255)
                                                                                                                                         tttAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGa             >  1:1887908/1‑150 (MQ=255)
                                                                                                                                            aaTTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAAtt          <  1:1740720/150‑1 (MQ=255)
                                                                                                                                             aTTTCTTCAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTg         >  1:3045423/1‑150 (MQ=255)
                                                                                                                                             aTTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTg         <  1:2604508/150‑1 (MQ=255)
                                                                                                                                             aTTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTg         >  1:3437607/1‑150 (MQ=255)
                                                                                                                                                tCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTGAGt      >  2:3558498/1‑150 (MQ=255)
                                                                                                                                                 cTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAAGTCAGAATATAACTTCGCGGTAAATGAATTGAGTa     <  1:3064265/150‑1 (MQ=255)
                                                                                                                                                   tAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTGAGTAAg   <  1:269218/150‑1 (MQ=255)
                                                                                                                                                    aaaTGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTGAGTAAGt  >  2:2483193/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTTAATACTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGCGTTAACAAAATACAGGTTGCGTTAACAACCAAGTTGAAATGATTTAATTTCTTAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTGAGTAAGT  >  CP009273/1099230‑1099527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A