Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F202 I0 R1	1397	275.7	9089454	95.2%	8653160	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,153,110	T→A	44.5%	intergenic (+80/‑215)	ycfH → / → ptsG	putative DNase/fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,153,110	0	T	A	44.5%	86.5 / inf	286	intergenic (+80/‑215)	ycfH/ptsG	putative DNase/fused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand): ref base T (82/77); new base A (63/64); total (145/141)
Fisher's exact test for biased strand distribution p-value = 8.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-02

Only 100 of 290 total aligned reads displayed.

AGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCG  >  CP009273/1152963‑1153257

aGGTAACCCCCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGatt                                                                                                                                                   <  2:295809/150‑1 (MQ=255)

aGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGatt                                                                                                                                                   <  1:1042104/150‑1 (MQ=255)

aGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGatt                                                                                                                                                   <  1:3318088/150‑1 (MQ=255)

    aaCCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGaaa                                                                                                                                               <  1:1112438/150‑1 (MQ=255)

      ccaccGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAata                                                                                                                                             <  1:1524414/150‑1 (MQ=255)

      ccaccGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAata                                                                                                                                             <  1:1661569/150‑1 (MQ=255)

        accGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATAtt                                                                                                                                           <  2:234886/150‑1 (MQ=255)

           gATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATtgtg                                                                                                                                        >  2:1558679/1‑150 (MQ=255)

           gATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATtgtg                                                                                                                                        >  2:550915/1‑150 (MQ=255)

           gATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATtgtg                                                                                                                                        >  2:134321/1‑150 (MQ=255)

              aaCTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACa                                                                                                                                     >  1:522354/1‑150 (MQ=255)

              aaCTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACa                                                                                                                                     >  2:3263922/1‑150 (MQ=255)

              aaCTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACa                                                                                                                                     >  2:2764633/1‑150 (MQ=255)

                cTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACata                                                                                                                                   >  1:263551/1‑150 (MQ=255)

                    gCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGtt                                                                                                                               <  1:1613629/150‑1 (MQ=255)

                      ccGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGtttt                                                                                                                             >  2:2152674/1‑150 (MQ=255)

                      ccGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGtttt                                                                                                                             >  1:3209093/1‑150 (MQ=255)

                      ccGTCTGTTTCACATCGACGCTTAACTCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAATCTCGTAATTAATGGATAAAACGATTAAATTTCAACGCCGAATATTGGGCGGTGAATAACCACGTTTGAAATATTGTTACATATGtttt                                                                                                                             <  1:2530438/150‑1 (MQ=255)

                      ccGTCTGGTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGtttt                                                                                                                             <  2:4288314/150‑1 (MQ=255)

                        gTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGt                                                                                                                           >  1:3876529/1‑150 (MQ=255)

                           tGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCaa                                                                                                                        <  2:97043/150‑1 (MQ=255)

                           tGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCaa                                                                                                                        <  2:3106083/150‑1 (MQ=255)

                              ttCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAAt                                                                                                                     <  1:822278/150‑1 (MQ=255)

                              ttCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAAt                                                                                                                     <  1:3102728/150‑1 (MQ=255)

                              ttCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAAt                                                                                                                     <  1:3903811/150‑1 (MQ=255)

                              ttCACATCGACGCGTCCCGCCTTCACTCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATGACTGGCTAAAACGAGTAAAGTTCACCGCCGAAAAATGGGCGGTGAATAACCACGTTTGAAATATTGTGACAGATGTTTTGTCAAAAt                                                                                                                     <  2:4381376/150‑1 (MQ=255)

                                  caTCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGc                                                                                                                 <  2:1277224/150‑1 (MQ=255)

                                      gACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGAGTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACt                                                                                                             >  2:2900396/1‑150 (MQ=255)

                                       aCGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACtt                                                                                                            >  2:2385531/1‑150 (MQ=255)

                                          cTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTGTTGTCAAAATGTGCAACTtctc                                                                                                         >  2:46919/1‑150 (MQ=255)

                                           ttCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTcc                                                                                                        <  2:3297757/150‑1 (MQ=255)

                                            tCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCa                                                                                                       >  1:3404239/1‑150 (MQ=255)

                                              ccGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATAGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAAt                                                                                                     >  2:4105464/1‑150 (MQ=255)

                                                   ttCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCt                                                                                                <  2:815389/150‑1 (MQ=255)

                                                       atccatccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAg                                                                                            <  1:2152674/150‑1 (MQ=255)

                                                         ccatccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGtt                                                                                          <  2:3356822/150‑1 (MQ=255)

                                                          catccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTg                                                                                         >  2:2620880/1‑150 (MQ=255)

                                                          catccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTg                                                                                         <  1:2246243/150‑1 (MQ=255)

                                                           atccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGa                                                                                        <  1:1989842/150‑1 (MQ=255)

                                                            tccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGaa                                                                                       >  2:144255/1‑150 (MQ=255)

                                                               gTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACg                                                                                    >  2:151887/1‑150 (MQ=255)

                                                                ttGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGt                                                                                   >  2:4259981/1‑150 (MQ=255)

                                                                tggAATGAGTTTTTTTAAAGCTCGTAATTAAAGGCTAAAACTAGTAAAGTTAACCGCCGAAAATTGGGCGGTTAATAACCACGTTTGAAATATTGTGACATATGTTTTGTAAAAATTTGCAACTTCTCAAATGATCTGAAGTTTAAAAGt                                                                                   >  2:2501544/3‑150 (MQ=255)

                                                                 tGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTg                                                                                  >  1:496030/1‑150 (MQ=255)

                                                                  gAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGa                                                                                 >  1:246719/1‑150 (MQ=255)

                                                                    aTGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATa                                                                               <  2:2069439/150‑1 (MQ=255)

                                                                      gAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGc                                                                             >  2:1859500/1‑150 (MQ=255)

                                                                      gAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGc                                                                             >  2:3521593/1‑150 (MQ=255)

                                                                       aGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGcc                                                                            >  2:1815752/1‑150 (MQ=255)

                                                                       aGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGcc                                                                            >  2:3439500/1‑150 (MQ=255)

                                                                               tAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTcaaaca                                                                    >  1:1778566/1‑150 (MQ=255)

                                                                               tAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTcaaaca                                                                    >  2:2010629/1‑150 (MQ=255)

                                                                                aaaGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTcaaacaa                                                                   >  2:1786118/1‑150 (MQ=255)

                                                                                aaaCCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTcaaacaa                                                                   >  2:1705431/1‑150 (MQ=255)

                                                                                    cTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTg                                                               >  2:3962489/1‑150 (MQ=255)

                                                                                       gTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCa                                                            >  1:3243063/1‑150 (MQ=255)

                                                                                       gTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCa                                                            >  1:3532323/1‑150 (MQ=255)

                                                                                           ttaatGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGa                                                        >  1:2508425/1‑150 (MQ=255)

                                                                                           ttaatGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGa                                                        <  2:3976672/150‑1 (MQ=255)

                                                                                             aatGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAat                                                      <  1:1281448/150‑1 (MQ=255)

                                                                                              atGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAatt                                                     <  2:522354/150‑1 (MQ=255)

                                                                                                  cTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTAttt                                                 <  2:3533556/150‑1 (MQ=255)

                                                                                                  cTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTAttt                                                 <  2:698051/150‑1 (MQ=255)

                                                                                                   tAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTAtttt                                                <  1:86335/150‑1 (MQ=255)

                                                                                                    aaaaCTAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTa                                               <  2:2884713/150‑1 (MQ=255)

                                                                                                     aaaCGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCAATTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTAc                                              <  1:702478/150‑1 (MQ=255)

                                                                                                        cGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTActct                                           >  2:1314928/1‑150 (MQ=255)

                                                                                                         gAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCtg                                          >  1:1712574/1‑150 (MQ=255)

                                                                                                          aGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCtgt                                         >  2:2713122/1‑150 (MQ=255)

                                                                                                            tAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTgtgt                                       <  2:3808740/150‑1 (MQ=255)

                                                                                                              aaGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGtaa                                     >  1:4349836/1‑150 (MQ=255)

                                                                                                               aGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTA‑TTTACTCTGTGtaata                                   <  2:3856988/150‑1 (MQ=255)

                                                                                                               aGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGtaat                                    <  1:2368886/150‑1 (MQ=255)

                                                                                                               aGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGtaat                                    <  2:2198537/150‑1 (MQ=255)

                                                                                                                gTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGtaata                                   >  2:3379939/1‑150 (MQ=255)

                                                                                                                 ttCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGtaataa                                  >  1:4129632/1‑150 (MQ=255)

                                                                                                                  tCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTaataaa                                 <  2:1572449/150‑1 (MQ=255)

                                                                                                                  tCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTaataaa                                 >  1:1944885/1‑150 (MQ=255)

                                                                                                                    aCCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTaataaata                               <  1:3330695/150‑1 (MQ=255)

                                                                                                                          gAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGgc                         <  1:3717055/150‑1 (MQ=255)

                                                                                                                          gAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGgc                         >  2:1104739/1‑150 (MQ=255)

                                                                                                                          gAAAATTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGgc                         <  1:3383773/150‑1 (MQ=255)

                                                                                                                           aaaaTTGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGgcg                        >  2:3252116/1‑150 (MQ=255)

                                                                                                                             aaTTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCt                      <  1:1409187/150‑1 (MQ=255)

                                                                                                                              aTTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCtt                     <  2:2992770/150‑1 (MQ=255)

                                                                                                                                tGGGCGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAg                   >  2:2606206/1‑150 (MQ=255)

                                                                                                                                 gggCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGa                  >  1:2418167/1‑150 (MQ=255)

                                                                                                                                 gggCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGa                  >  1:2417565/1‑150 (MQ=255)

                                                                                                                                  ggCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGAt                 >  1:2760547/1‑150 (MQ=255)

                                                                                                                                    cGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGc               >  2:3653085/1‑150 (MQ=255)

                                                                                                                                    cGGTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGc               >  1:1132170/1‑150 (MQ=255)

                                                                                                                                     ggTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGcc              <  2:834148/150‑1 (MQ=255)

                                                                                                                                     ggTGAATAACCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGcc              >  2:3668562/1‑150 (MQ=255)

                                                                                                                                      gTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGccc             >  2:605461/1‑150 (MQ=255)

                                                                                                                                          aTAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTa         >  1:2215659/1‑150 (MQ=255)

                                                                                                                                            aaCCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTaca       >  2:3706615/1‑150 (MQ=255)

                                                                                                                                             aCCACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTacac      <  1:1558679/150‑1 (MQ=255)

                                                                                                                                              ccACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACg     >  2:2538894/1‑150 (MQ=255)

                                                                                                                                              ccACGATTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGACACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACg     >  2:1003655/1‑150 (MQ=255)

                                                                                                                                                 cGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCg  <  2:1217426/150‑1 (MQ=255)

AGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCG  >  CP009273/1152963‑1153257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A