Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,238,721 C→A 5.8% A29E (GCG→GAG)  emtA → lytic murein endotransglycosylase E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,238,7210CA 5.8% 844.8 / 19.7 225A29E (GCG→GAG) emtAlytic murein endotransglycosylase E
Reads supporting (aligned to +/- strand):  ref base C (130/82);  new base A (3/10);  total (133/92)
Fisher's exact test for biased strand distribution p-value = 8.53e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 230 total aligned reads displayed.

ATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGTAAATCGAATGCCATT  >  CP009273/1238572‑1238869
                                                                                                                                                     |                                                                                                                                                    
 tCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAAcgcg                                                                                                                                                     <  2:163735/150‑1 (MQ=255)
 tCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAAcgcg                                                                                                                                                     >  2:2409107/1‑150 (MQ=255)
  cATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGa                                                                                                                                                    >  1:3043830/1‑150 (MQ=255)
    tGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGaaa                                                                                                                                                  >  2:2566009/1‑150 (MQ=255)
     gCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAg                                                                                                                                                 <  1:2346877/150‑1 (MQ=255)
       cGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGtt                                                                                                                                               <  1:291366/150‑1 (MQ=255)
          ttAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCg                                                                                                                                            >  2:4367740/1‑150 (MQ=255)
          ttAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCg                                                                                                                                            >  1:2138939/1‑150 (MQ=255)
             ggTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATAACTATACGAACCCGCCGTGGAACGCGACAGTTCCGGTg                                                                                                                                         >  2:3997965/1‑150 (MQ=255)
                gtgCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCaa                                                                                                                                      <  1:182519/150‑1 (MQ=255)
                 tgCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAAc                                                                                                                                     <  2:1073793/150‑1 (MQ=255)
                    cGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTg                                                                                                                                  >  1:1164670/1‑150 (MQ=255)
                    cGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTg                                                                                                                                  >  1:3580739/1‑150 (MQ=255)
                    cGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTg                                                                                                                                  >  1:3435040/1‑150 (MQ=255)
                      ttGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCg                                                                                                                                >  1:1089393/1‑150 (MQ=255)
                       tGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGa                                                                                                                               <  1:1636022/150‑1 (MQ=255)
                        gTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATAAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGAt                                                                                                                              <  1:1906098/150‑1 (MQ=255)
                         tCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATg                                                                                                                             <  2:4487611/150‑1 (MQ=255)
                             cTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGt                                                                                                                         >  2:208419/1‑150 (MQ=255)
                             cTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGt                                                                                                                         >  1:4364384/1‑150 (MQ=255)
                             cTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGt                                                                                                                         >  2:2934269/1‑150 (MQ=255)
                             cTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGAGAAAGTTCCGGTGCAACGTGCGATGCAGt                                                                                                                         <  2:3103481/150‑1 (MQ=255)
                              tCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTg                                                                                                                        <  1:2143718/150‑1 (MQ=255)
                                 aCGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGAt                                                                                                                     <  1:2825570/150‑1 (MQ=255)
                                 aCGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGAGGAACGCGAAAGTTCCGGTGCAACGAGCGATGCAGTGGAt                                                                                                                     >  2:1871836/1‑150 (MQ=255)
                                  cGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATg                                                                                                                    >  2:492145/1‑150 (MQ=255)
                                     cGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGCTGCCa                                                                                                                 >  2:2500795/1‑150 (MQ=255)
                                        ttCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATa                                                                                                              <  2:4015859/150‑1 (MQ=255)
                                              gCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAg                                                                                                        >  1:3747512/1‑150 (MQ=255)
                                               cTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGa                                                                                                       >  1:2800458/1‑150 (MQ=255)
                                                 ataAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGaaa                                                                                                     >  1:473689/1‑150 (MQ=255)
                                                    aGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGcc                                                                                                  <  1:1606771/150‑1 (MQ=255)
                                                    aGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGcc                                                                                                  <  2:3638632/150‑1 (MQ=255)
                                                     ggATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCg                                                                                                 >  2:1593611/1‑150 (MQ=255)
                                                      gATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCgg                                                                                                >  2:2282486/1‑150 (MQ=255)
                                                       aTAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGt                                                                                               <  1:902696/150‑1 (MQ=255)
                                                         agaagaAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACACGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCTATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGc                                                                                             >  2:2984818/1‑150 (MQ=255)
                                                             gaagTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGcc                                                                                         <  1:1628652/150‑1 (MQ=255)
                                                             gaagTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGcc                                                                                         >  2:2183813/1‑150 (MQ=255)
                                                              aagTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCt                                                                                        <  1:1140632/150‑1 (MQ=255)
                                                                  gAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTgggg                                                                                    <  2:3282174/150‑1 (MQ=255)
                                                                   aaaTTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGc                                                                                   <  2:1949675/150‑1 (MQ=255)
                                                                    aaTTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCg                                                                                  >  2:3989426/1‑150 (MQ=255)
                                                                        gagaTGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGa                                                                              >  1:1997096/1‑150 (MQ=255)
                                                                        gagaTGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGa                                                                              >  1:278681/1‑150 (MQ=255)
                                                                        gagaTGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGa                                                                              >  1:2900896/1‑150 (MQ=255)
                                                                         agaTGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGAt                                                                             >  1:2581870/1‑150 (MQ=255)
                                                                         agaTGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGAt                                                                             >  1:306490/1‑150 (MQ=255)
                                                                             ggTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCcac                                                                         <  1:3891949/150‑1 (MQ=255)
                                                                              gTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCcaca                                                                        >  2:3155375/1‑150 (MQ=255)
                                                                                ttGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAAt                                                                      <  1:1449249/150‑1 (MQ=255)
                                                                                ttGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAAt                                                                      >  2:2235320/1‑150 (MQ=255)
                                                                                    cTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGAt                                                                  <  2:2516171/150‑1 (MQ=255)
                                                                                     tttttGATTGTGTTATTCGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATc                                                                 <  1:3014158/150‑1 (MQ=255)
                                                                                          gATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGc                                                            >  1:520583/1‑150 (MQ=255)
                                                                                          gATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGc                                                            >  1:4454714/1‑150 (MQ=255)
                                                                                           aTTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCg                                                           >  2:4155271/1‑150 (MQ=255)
                                                                                            ttGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGa                                                          >  2:4180827/1‑150 (MQ=255)
                                                                                                gtTATTAGCGGGGTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCT‑GGGCGTCGATCCACAATTGATCACGGCGATTATc                                                     <  2:2498839/150‑1 (MQ=255)
                                                                                                 ttattaGCGGGTTGTTCATCAAAGCATGACTCTACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATc                                                     <  2:2498853/150‑1 (MQ=255)
                                                                                                  tattaGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCg                                                    >  1:226349/1‑150 (MQ=255)
                                                                                                  tattaGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCg                                                    <  1:4380004/150‑1 (MQ=255)
                                                                                                    ttaGCGGGTTGTTCATCAAAGCTTGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCt                                                  <  1:3986985/150‑1 (MQ=255)
                                                                                                     taGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTa                                                 >  1:1946443/1‑150 (MQ=255)
                                                                                                      aGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTAt                                                <  2:2581877/150‑1 (MQ=255)
                                                                                                      aGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTAt                                                <  2:2581343/150‑1 (MQ=255)
                                                                                                      aGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTAt                                                >  2:1063752/1‑150 (MQ=255)
                                                                                                        cGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCg                                              >  1:3873915/1‑150 (MQ=255)
                                                                                                         gggTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTCTCGCTATCGa                                             >  2:1013669/1‑150 (MQ=255)
                                                                                                           gttgttCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAAt                                           >  2:1491139/1‑150 (MQ=255)
                                                                                                           gttgttCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGATCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGACGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAAt                                           >  1:577616/1‑150 (MQ=255)
                                                                                                             tgttCATCAAATCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCg                                         <  2:436460/150‑1 (MQ=255)
                                                                                                             tgttCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCg                                         <  2:434972/150‑1 (MQ=255)
                                                                                                              gttCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCgg                                        <  2:1233904/150‑1 (MQ=255)
                                                                                                                       aGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATc                               <  1:1485561/150‑1 (MQ=255)
                                                                                                                       aGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATc                               <  1:2498583/150‑1 (MQ=255)
                                                                                                                           tGACTATACGAACCCGCCGTGGACCGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGaa                           <  2:3231635/150‑1 (MQ=255)
                                                                                                                           tGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGaa                           >  1:1378116/1‑150 (MQ=255)
                                                                                                                            gACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAAc                          >  2:2091718/1‑150 (MQ=255)
                                                                                                                             aCTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAAcg                         <  1:2553562/150‑1 (MQ=255)
                                                                                                                             aCTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAAcg                         >  1:1955413/1‑150 (MQ=255)
                                                                                                                             aCTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAAcg                         <  1:2408050/150‑1 (MQ=255)
                                                                                                                             aCTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAAcg                         >  1:4202093/1‑150 (MQ=255)
                                                                                                                             aCTATACGAACCCGCCGTGGAACGAGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGTCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAAcg                         <  2:1721854/150‑1 (MQ=255)
                                                                                                                              cTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAAcgc                        <  2:725068/150‑1 (MQ=255)
                                                                                                                               tataCGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAAcgcg                       >  2:613997/1‑150 (MQ=255)
                                                                                                                               tataCGAACCCGCCGTGGAACGAGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAAcgcg                       <  2:2781158/150‑1 (MQ=255)
                                                                                                                               tataCGAACCCGCCGTGGAACGAGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAAcgcg                       <  2:2780948/150‑1 (MQ=255)
                                                                                                                                 taCGAACCCGCCGTGGAACGAGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAACGCGGt                     <  1:3460264/150‑1 (MQ=255)
                                                                                                                                     aaCCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGt                 >  1:4309102/1‑150 (MQ=255)
                                                                                                                                     aaCCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGt                 >  1:4309053/1‑150 (MQ=255)
                                                                                                                                     aaCCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGt                 >  1:4309030/1‑150 (MQ=255)
                                                                                                                                          gccgTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCTCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGTAAATc            <  1:4090831/150‑1 (MQ=255)
                                                                                                                                          gccgTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGTAAATc            <  1:1211334/150‑1 (MQ=255)
                                                                                                                                          gccgTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATACCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGTAAATc            >  1:2728702/1‑150 (MQ=255)
                                                                                                                                           ccgTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAGGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGTAAATCg           >  2:1929854/1‑150 (MQ=255)
                                                                                                                                           ccgTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGTAAATCg           >  2:1929805/1‑150 (MQ=255)
                                                                                                                                           ccgTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGTAAATCg           <  1:943848/150‑1 (MQ=255)
                                                                                                                                                gAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGTAAATCGAATGc      <  2:2854676/150‑1 (MQ=255)
                                                                                                                                                    gcgAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGTAAATCGAATGCCAtt  >  2:442537/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATTATCGCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGTAAATCGAATGCCATT  >  CP009273/1238572‑1238869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A