Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,561,151 C→A 42.1% D83Y (GAT→TAT)  dosC ← diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,561,1510CA42.1% 265.3 / inf 285D83Y (GAT→TAT) dosCdiguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator
Reads supporting (aligned to +/- strand):  ref base C (81/84);  new base A (66/54);  total (147/138)
Fisher's exact test for biased strand distribution p-value = 3.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

Only 100 of 294 total aligned reads displayed.

TTCTGCGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATGCG  >  CP009273/1561002‑1561300
                                                                                                                                                     |                                                                                                                                                     
ttCTGCGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATc                                                                                                                                                       >  2:2686637/1‑150 (MQ=255)
ttCTGCGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATc                                                                                                                                                       >  1:2035608/1‑150 (MQ=255)
ttCTGCGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATc                                                                                                                                                       >  1:2035652/1‑150 (MQ=255)
   tGCGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTc                                                                                                                                                    >  1:3748274/1‑150 (MQ=255)
     cggcggAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCaa                                                                                                                                                  >  1:967323/1‑150 (MQ=255)
        cggAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCt                                                                                                                                               <  2:1608320/150‑1 (MQ=255)
         ggAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTg                                                                                                                                              >  1:951661/1‑150 (MQ=255)
          gAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTgg                                                                                                                                             >  2:3401800/1‑150 (MQ=255)
          gAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTgg                                                                                                                                             <  2:1779698/150‑1 (MQ=255)
            ataatCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACAGAGTCAACCTGGGc                                                                                                                                           <  2:4199958/150‑1 (MQ=255)
                tCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGaa                                                                                                                                       >  2:2851700/1‑150 (MQ=255)
                   gaagagaagaTCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGc                                                                                                                                    >  1:4522189/1‑150 (MQ=255)
                    aagagaagaTCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCa                                                                                                                                   >  2:1363232/1‑150 (MQ=255)
                        gaagaTCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGtt                                                                                                                               <  2:2140258/150‑1 (MQ=255)
                            aTCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTtaata                                                                                                                           >  1:1699132/1‑150 (MQ=255)
                              cACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTaataat                                                                                                                         <  2:2661192/150‑1 (MQ=255)
                              cACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTaataat                                                                                                                         <  2:2136203/150‑1 (MQ=255)
                                 cGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCa                                                                                                                      <  1:2905177/150‑1 (MQ=255)
                                   gATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGc                                                                                                                    <  1:3609350/150‑1 (MQ=255)
                                    aTAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCg                                                                                                                   <  2:3296579/150‑1 (MQ=255)
                                    aTAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCg                                                                                                                   >  1:4337038/1‑150 (MQ=255)
                                    aTAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCg                                                                                                                   <  1:789281/150‑1 (MQ=255)
                                     tAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGt                                                                                                                  >  1:3179600/1‑150 (MQ=255)
                                     tAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGt                                                                                                                  <  1:2086385/150‑1 (MQ=255)
                                       gatgATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTc                                                                                                                <  2:201173/147‑1 (MQ=255)
                                       gagGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTc                                                                                                                >  1:695697/1‑150 (MQ=255)
                                          gATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCAt                                                                                                             >  2:4010757/1‑150 (MQ=255)
                                              tttttCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCa                                                                                                         <  1:3675621/150‑1 (MQ=255)
                                              tttttCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCa                                                                                                         >  1:4325242/1‑150 (MQ=255)
                                              tttttCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCa                                                                                                         >  1:2566222/1‑150 (MQ=255)
                                              tttttCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGAAATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCa                                                                                                         >  1:4509523/1‑150 (MQ=255)
                                                tttCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCAct                                                                                                       <  1:3875357/150‑1 (MQ=255)
                                                 ttCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCActc                                                                                                      >  2:1470365/1‑150 (MQ=255)
                                                  tCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCActct                                                                                                     >  2:591394/1‑150 (MQ=255)
                                                   cAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCtt                                                                                                    <  2:564436/150‑1 (MQ=255)
                                                     gCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTACGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCa                                                                                                  <  2:4135095/150‑1 (MQ=255)
                                                         ccGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTg                                                                                              >  1:409256/1‑150 (MQ=255)
                                                          cGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGc                                                                                             >  1:1144506/1‑150 (MQ=255)
                                                          cGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGc                                                                                             >  2:24229/1‑150 (MQ=255)
                                                           gAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGcc                                                                                            >  2:801485/1‑150 (MQ=255)
                                                           gAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGcc                                                                                            >  2:3081387/1‑150 (MQ=255)
                                                           gAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGcc                                                                                            >  1:4117301/1‑150 (MQ=255)
                                                             aaCCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGc                                                                                          >  2:3408660/1‑150 (MQ=255)
                                                              aCCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCt                                                                                         <  1:2054237/150‑1 (MQ=255)
                                                              aCCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCt                                                                                         >  2:1856464/1‑150 (MQ=255)
                                                              aCCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCt                                                                                         <  1:597896/150‑1 (MQ=255)
                                                               ccccATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTc                                                                                        >  1:2418345/1‑150 (MQ=255)
                                                                         aCAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCa                                                                              >  2:3791003/1‑150 (MQ=255)
                                                                                   ccGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGa                                                                    >  1:1745499/1‑150 (MQ=255)
                                                                                    cGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGaa                                                                   >  2:1087085/1‑150 (MQ=255)
                                                                                      gAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAAtt                                                                 <  2:2192744/150‑1 (MQ=255)
                                                                                      gAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAAtt                                                                 <  1:3138552/150‑1 (MQ=255)
                                                                                       aaTTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAAttc                                                                >  2:1632379/1‑150 (MQ=255)
                                                                                        aTTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAAttct                                                               <  2:4421873/150‑1 (MQ=255)
                                                                                        aTTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAAttct                                                               <  1:2970368/150‑1 (MQ=255)
                                                                                          tCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAAttcttc                                                             <  2:3457931/150‑1 (MQ=255)
                                                                                           ccTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCg                                                            >  1:4409087/1‑150 (MQ=255)
                                                                                            cTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCgg                                                           >  1:822107/1‑150 (MQ=255)
                                                                                             tATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCGCTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGc                                                          >  1:1077731/1‑150 (MQ=255)
                                                                                             tATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAAtt                                                                 >  2:3187687/1‑143 (MQ=255)
                                                                                             tATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGc                                                          >  1:377052/1‑150 (MQ=255)
                                                                                             tATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGc                                                          >  1:1077776/1‑150 (MQ=255)
                                                                                                  gggCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCg                                                     >  1:319317/1‑150 (MQ=255)
                                                                                                  gggCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCg                                                     >  1:2144073/1‑150 (MQ=255)
                                                                                                   ggCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCgg                                                    <  1:2796266/150‑1 (MQ=255)
                                                                                                   ggCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCgg                                                    <  2:1491058/150‑1 (MQ=255)
                                                                                                    gCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCggg                                                   <  1:1662585/150‑1 (MQ=255)
                                                                                                     cATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGt                                                  <  1:2339753/150‑1 (MQ=255)
                                                                                                     cATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGt                                                  <  1:1849756/150‑1 (MQ=255)
                                                                                                     cATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGt                                                  >  1:579716/1‑150 (MQ=255)
                                                                                                      aTGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTc                                                 <  1:2701532/150‑1 (MQ=255)
                                                                                                      aTGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTc                                                 <  1:484793/150‑1 (MQ=255)
                                                                                                      aTGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTc                                                 <  1:484410/150‑1 (MQ=255)
                                                                                                         cACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGAt                                              <  2:1145256/150‑1 (MQ=255)
                                                                                                           cTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGc                                            <  1:3401800/150‑1 (MQ=255)
                                                                                                            ttCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCg                                           <  2:2333892/150‑1 (MQ=255)
                                                                                                             tCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCgg                                          <  1:2899203/150‑1 (MQ=255)
                                                                                                             tCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCgg                                          <  2:3608576/150‑1 (MQ=255)
                                                                                                              ccGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGa                                         <  1:2888717/150‑1 (MQ=255)
                                                                                                               cgcgACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGAc                                        <  2:638959/150‑1 (MQ=255)
                                                                                                                 cgACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACaa                                      >  1:863176/1‑150 (MQ=255)
                                                                                                                   aCGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAAtt                                    <  1:2686637/150‑1 (MQ=255)
                                                                                                                      gTATGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGa                                 >  1:529085/1‑150 (MQ=255)
                                                                                                                         tGCTGGATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAAGCCAGCGTTCCAGAGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGATTTCTTCGGCATGCGGGTCGATGCGGACAATTCGATaa                              <  1:4439679/150‑1 (MQ=255)
                                                                                                                           cTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATaaaa                            >  1:1031859/1‑150 (MQ=255)
                                                                                                                           cTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATaaaa                            >  1:3093218/1‑150 (MQ=255)
                                                                                                                             ggATTTGTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACACTTCGATAAAACt                          >  1:3726859/1‑150 (MQ=255)
                                                                                                                               aTTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTACGGCATGCGGGTCGATGCTGACAATTCGATAAAACTCg                        >  1:2246655/1‑150 (MQ=255)
                                                                                                                                   gTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATAc                    >  2:2950868/1‑150 (MQ=255)
                                                                                                                                   gTATTAGCCTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATAc                    >  2:2950801/1‑150 (MQ=255)
                                                                                                                                        aGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGa               <  1:3557098/150‑1 (MQ=255)
                                                                                                                                        aGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAAATCGATACTCAGa               >  1:662566/1‑150 (MQ=255)
                                                                                                                                        aGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATACAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCACTAATTCTTCGGCATGCGGGTCGATGCTGACAATTCGATAAAACTCGATACTCAGa               <  2:3413486/150‑1 (MQ=255)
                                                                                                                                         gCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGAt              <  1:2823440/150‑1 (MQ=255)
                                                                                                                                           cTTTCGACATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATaa            >  1:2858101/1‑150 (MQ=255)
                                                                                                                                                 aCATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCa      >  2:2868017/1‑150 (MQ=255)
                                                                                                                                                 aCATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCa      <  2:3389435/150‑1 (MQ=255)
                                                                                                                                                  cATAGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCAt     >  2:4180023/1‑150 (MQ=255)
                                                                                                                                                    tCGTCAAACTGGGCAGTAAGCACTTTAATAATCCATCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCTGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATgc   <  2:3736914/150‑1 (MQ=255)
                                                                                                                                                     cGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATgcg  >  1:1436853/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCTGCGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATGCG  >  CP009273/1561002‑1561300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A