Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,370,987 C→A 91.6% E29* (GAA→TAA)  menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,370,9870CA91.6% 625.5 / 51.0 228E29* (GAA→TAA) menH2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base C (4/15);  new base A (110/99);  total (114/114)
Fisher's exact test for biased strand distribution p-value = 1.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01

Only 100 of 228 total aligned reads displayed.

GATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCA  >  CP009273/2370841‑2371132
                                                                                                                                                  |                                                                                                                                                 
   gttgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCa                                                                                                                                             >  2:828382/1‑150 (MQ=255)
     tgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAAt                                                                                                                                           >  1:1324885/1‑150 (MQ=255)
     tgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAAt                                                                                                                                           <  1:738231/150‑1 (MQ=255)
     tgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAAt                                                                                                                                           >  2:1273409/1‑150 (MQ=255)
      gtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATc                                                                                                                                          <  2:3421293/150‑1 (MQ=255)
      gtAACGAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATc                                                                                                                                          <  2:3154918/150‑1 (MQ=255)
          ctaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCg                                                                                                                                      >  2:208185/1‑150 (MQ=255)
           taacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCgg                                                                                                                                     <  1:3572599/150‑1 (MQ=255)
            aacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGa                                                                                                                                    <  1:1389742/150‑1 (MQ=255)
             acCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGaa                                                                                                                                   <  2:806254/150‑1 (MQ=255)
              ccAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGaaa                                                                                                                                  >  1:3521007/1‑150 (MQ=255)
               cAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGaaaa                                                                                                                                 <  1:2762340/150‑1 (MQ=255)
               cAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGaaaa                                                                                                                                 <  1:1024600/150‑1 (MQ=255)
               cAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGaaaa                                                                                                                                 >  1:4283435/1‑150 (MQ=255)
                  ggTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAcc                                                                                                                              <  1:3646012/150‑1 (MQ=255)
                  ggTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAAcc                                                                                                                              <  2:862141/150‑1 (MQ=255)
                   gTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCg                                                                                                                             >  2:2343284/1‑150 (MQ=255)
                   gTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCg                                                                                                                             >  2:39371/1‑150 (MQ=255)
                    ttttACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGt                                                                                                                            >  2:4508864/1‑150 (MQ=255)
                       tACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCa                                                                                                                         <  1:715168/150‑1 (MQ=255)
                       tACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCa                                                                                                                         <  1:1193447/150‑1 (MQ=255)
                       tACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCa                                                                                                                         >  2:1626485/1‑150 (MQ=255)
                        aCGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCaa                                                                                                                        <  1:546621/150‑1 (MQ=255)
                        aCGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCaa                                                                                                                        <  1:1797833/150‑1 (MQ=255)
                        aCGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCaa                                                                                                                        >  2:1159746/1‑150 (MQ=255)
                        aCGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCaa                                                                                                                        >  2:1159754/1‑150 (MQ=255)
                        aCGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATAAACAAACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCaa                                                                                                                        >  2:1159712/1‑150 (MQ=255)
                         cGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCaaa                                                                                                                       >  1:2161099/1‑150 (MQ=255)
                          gCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCaaaa                                                                                                                      >  2:4378754/1‑150 (MQ=255)
                           cAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCaaaaa                                                                                                                     >  1:1612348/1‑150 (MQ=255)
                               aaGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcc                                                                                                                 >  1:1620771/1‑150 (MQ=255)
                               aaGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGAGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcc                                                                                                                 <  1:3468089/150‑1 (MQ=255)
                                   cGGTGACAACATCAAATCCAACGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagcc                                                                                                             <  2:3670242/150‑1 (MQ=255)
                                    ggTGACATCATCAAATCCATCGACGCTAATCGCCTCCTAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTATGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagcca                                                                                                            <  2:3134647/150‑1 (MQ=255)
                                    ggTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagcca                                                                                                            <  1:2681064/150‑1 (MQ=255)
                                     gTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagccag                                                                                                           <  1:2415551/150‑1 (MQ=255)
                                        aCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGt                                                                                                        >  2:249731/1‑150 (MQ=255)
                                          atcatcAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTaa                                                                                                      >  1:2565480/1‑150 (MQ=255)
                                           tcatcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTaaa                                                                                                     >  2:3702715/1‑150 (MQ=255)
                                            catcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAc                                                                                                    >  1:1905064/1‑150 (MQ=255)
                                             atcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACATCCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGAGCAAAAACACCAGCCAGGGTAAAcc                                                                                                   <  1:4436330/150‑1 (MQ=255)
                                             atcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCCGGGTAAAcc                                                                                                   >  2:3118338/1‑150 (MQ=255)
                                              tcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCt                                                                                                  <  1:4165735/150‑1 (MQ=255)
                                              tcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCt                                                                                                  >  1:3081846/1‑150 (MQ=255)
                                               caAATCCATCGACGCTATTCGCCGCCGACCCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCACACGCCTCGCCCACTTCTTGCCCTTAGTGGCAATCGCCGGAAAAACCGTGCACAAACACCCGCCAGGGTCAACCTg                                                                                                 >  2:2099374/1‑150 (MQ=255)
                                                aaaTCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTgg                                                                                                >  2:1480856/1‑150 (MQ=255)
                                                 aaTCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGt                                                                                               >  1:3826408/1‑150 (MQ=255)
                                                  aTCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGtt                                                                                              >  2:4543929/1‑150 (MQ=255)
                                                    ccATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGtttt                                                                                            <  1:2642244/150‑1 (MQ=255)
                                                    ccATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGtttt                                                                                            <  2:1752329/150‑1 (MQ=255)
                                                     cATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTc                                                                                           <  1:249771/150‑1 (MQ=255)
                                                      aTCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTcc                                                                                          >  2:2158737/1‑150 (MQ=255)
                                                       tCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCg                                                                                         <  2:1523385/150‑1 (MQ=255)
                                                          aCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCgtgt                                                                                      <  2:272892/150‑1 (MQ=255)
                                                            gCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGttt                                                                                    <  2:1697419/150‑1 (MQ=255)
                                                             cTACTCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGtttt                                                                                   <  2:2566485/150‑1 (MQ=255)
                                                             cTAAGCGCCGCCGAACCACCGTGACCTGGTAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGtttt                                                                                   <  2:2364407/150‑1 (MQ=255)
                                                              tAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTg                                                                                  >  1:3944142/1‑150 (MQ=255)
                                                                 tCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCt                                                                               <  2:1620757/150‑1 (MQ=255)
                                                                  cgccgcCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTg                                                                              <  2:3533716/150‑1 (MQ=255)
                                                                    ccgccgAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTgcg                                                                            >  2:1057845/1‑150 (MQ=255)
                                                                            ccaccGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAgg                                                                    >  1:197521/1‑150 (MQ=255)
                                                                                cGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCa                                                                <  2:2556897/150‑1 (MQ=255)
                                                                                cGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCa                                                                <  2:1666368/150‑1 (MQ=255)
                                                                                cGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCa                                                                <  2:3924883/150‑1 (MQ=255)
                                                                                   gACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATaa                                                             <  1:3160779/150‑1 (MQ=255)
                                                                                        gggAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGc                                                        >  1:4141886/1‑150 (MQ=255)
                                                                                           agaTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTa                                                     <  1:4520492/150‑1 (MQ=255)
                                                                                           agaTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTa                                                     >  1:3767383/1‑150 (MQ=255)
                                                                                            gaTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTAc                                                    >  2:3378046/1‑150 (MQ=255)
                                                                                            gaTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCAAAAATGGCTTAc                                                    >  1:3008807/1‑150 (MQ=255)
                                                                                             aTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTAcc                                                   >  2:4243460/1‑150 (MQ=255)
                                                                                               cAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTg                                                 <  1:1464471/150‑1 (MQ=255)
                                                                                                aaCATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTgc                                                <  1:2084116/150‑1 (MQ=255)
                                                                                                 aCATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTgcg                                               <  1:1414627/150‑1 (MQ=255)
                                                                                                      cAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGa                                          >  1:3550022/1‑150 (MQ=255)
                                                                                                       aaCCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTCAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGaa                                         >  2:2390792/1‑150 (MQ=255)
                                                                                                       aaCCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGaa                                         >  2:3207775/1‑150 (MQ=255)
                                                                                                          cGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATGAGGGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGtt                                      <  2:475330/150‑1 (MQ=255)
                                                                                                           gTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTg                                     >  2:2834292/1‑150 (MQ=255)
                                                                                                             gAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCt                                   <  1:4292127/150‑1 (MQ=255)
                                                                                                             gAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCt                                   <  1:4001617/150‑1 (MQ=255)
                                                                                                              agtagtCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTg                                  >  1:3193193/1‑150 (MQ=255)
                                                                                                              agtagtCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTg                                  >  1:466858/1‑150 (MQ=255)
                                                                                                                tagtCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGa                                <  1:38729/150‑1 (MQ=255)
                                                                                                                  gtCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGc                              <  1:516489/150‑1 (MQ=255)
                                                                                                                     ggCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGtt                           >  2:2257564/1‑150 (MQ=255)
                                                                                                                          aCGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTgcgc                      >  1:1346807/1‑150 (MQ=255)
                                                                                                                           cGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCa                     <  2:1218628/150‑1 (MQ=255)
                                                                                                                            gCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCAc                    >  2:3908762/1‑150 (MQ=255)
                                                                                                                              cTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCa                  <  2:1400249/150‑1 (MQ=255)
                                                                                                                                cGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATc                >  1:3429046/1‑150 (MQ=255)
                                                                                                                                  cccACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCAGCATCgg              <  2:2899037/150‑1 (MQ=255)
                                                                                                                                  cccACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCgg              >  2:3766359/1‑150 (MQ=255)
                                                                                                                                      cttcttGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTc          <  2:1334498/150‑1 (MQ=255)
                                                                                                                                        tcttGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGt        >  2:291077/1‑150 (MQ=255)
                                                                                                                                        tcttGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGt        >  2:1191664/1‑150 (MQ=255)
                                                                                                                                           tGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTaa     <  1:4416599/150‑1 (MQ=255)
                                                                                                                                            gCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAac    >  1:4530968/1‑150 (MQ=255)
                                                                                                                                            gCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAac    >  1:1427791/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCA  >  CP009273/2370841‑2371132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACC  >  CP009273/2370841‑2371134
                                                                                                                                                  |                                                                                                                                                   
GATGTTGTAACTAACCAAGGTTTAACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTG                                                                                                                                                  <  SRR19973076.2204472/150‑1 (MQ=60)
 ATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGG                                                                                                                                                 >  SRR19973076.3634661/1‑150 (MQ=60)
  TGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGC                                                                                                                                                <  SRR19973076.2097324/150‑1 (MQ=60)
     TGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAAT                                                                                                                                             <  SRR19973076.739327/150‑1 (MQ=60)
     TGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAAT                                                                                                                                             >  SRR19973076.1326835/1‑150 (MQ=60)
     TGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAAT                                                                                                                                             <  SRR19973076.739283/150‑1 (MQ=60)
        AACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGC                                                                                                                                          >  SRR19973076.1920608/1‑150 (MQ=60)
           TAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGG                                                                                                                                       <  SRR19973076.3577790/150‑1 (MQ=60)
           TAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGG                                                                                                                                       >  SRR19973076.1336461/1‑150 (MQ=60)
            AACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCCCTTCTTGCCCTTAGTGGCACTCGCCGGC                                                                                                                                      >  SRR19973076.3848524/1‑150 (MQ=60)
            AACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGA                                                                                                                                      <  SRR19973076.1391793/150‑1 (MQ=60)
            AACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGA                                                                                                                                      >  SRR19973076.3848111/1‑150 (MQ=60)
            AACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGA                                                                                                                                      >  SRR19973076.3882966/1‑150 (MQ=60)
              CCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAA                                                                                                                                    >  SRR19973076.3526580/1‑150 (MQ=60)
              CCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAA                                                                                                                                    >  SRR19973076.3526115/1‑150 (MQ=60)
              CCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAA                                                                                                                                    >  SRR19973076.3312501/1‑150 (MQ=60)
               CAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAA                                                                                                                                   <  SRR19973076.2766351/150‑1 (MQ=60)
               CAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAA                                                                                                                                   <  SRR19973076.1026126/150‑1 (MQ=60)
               CAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAA                                                                                                                                   >  SRR19973076.4289762/1‑150 (MQ=60)
                  GGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACC                                                                                                                                <  SRR19973076.3651316/150‑1 (MQ=60)
                       TACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCA                                                                                                                           <  SRR19973076.1195253/150‑1 (MQ=60)
                       TACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCA                                                                                                                           <  SRR19973076.716234/150‑1 (MQ=60)
                       TACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCA                                                                                                                           <  SRR19973076.716232/150‑1 (MQ=60)
                       TACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCA                                                                                                                           <  SRR19973076.1195220/150‑1 (MQ=60)
                       TACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCA                                                                                                                           >  SRR19973076.4021843/1‑150 (MQ=60)
                        ACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAA                                                                                                                          <  SRR19973076.547482/150‑1 (MQ=60)
                        ACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAA                                                                                                                          <  SRR19973076.1800427/150‑1 (MQ=60)
                        ACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAA                                                                                                                          <  SRR19973076.4056905/150‑1 (MQ=60)
                         CGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAA                                                                                                                         >  SRR19973076.2164226/1‑150 (MQ=60)
                           CAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAA                                                                                                                       >  SRR19973076.1614711/1‑150 (MQ=60)
                           CAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAA                                                                                                                       >  SRR19973076.1614683/1‑150 (MQ=60)
                               AAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACC                                                                                                                   >  SRR19973076.1623145/1‑150 (MQ=60)
                               AAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACC                                                                                                                   >  SRR19973076.1623131/1‑150 (MQ=60)
                               AAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGAGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACC                                                                                                                   <  SRR19973076.3473109/150‑1 (MQ=60)
                                    GGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCA                                                                                                              <  SRR19973076.2684963/150‑1 (MQ=60)
                                    GGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCA                                                                                                              <  SRR19973076.1401286/150‑1 (MQ=60)
                                    GGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCA                                                                                                              <  SRR19973076.2684924/150‑1 (MQ=60)
                                    GGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCA                                                                                                              <  SRR19973076.2684956/150‑1 (MQ=60)
                                     GTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAG                                                                                                             <  SRR19973076.2419031/150‑1 (MQ=60)
                                       GACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGG                                                                                                           <  SRR19973076.4539234/150‑1 (MQ=60)
                                        ACATCATCAAATCCATCGACGGTAGTCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGT                                                                                                          >  SRR19973076.3693587/1‑150 (MQ=60)
                                        ACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGT                                                                                                          >  SRR19973076.1420020/1‑150 (MQ=60)
                                          ATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAA                                                                                                        >  SRR19973076.2569218/1‑150 (MQ=60)
                                          ATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAA                                                                                                        >  SRR19973076.2569212/1‑150 (MQ=60)
                                          ATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAA                                                                                                        >  SRR19973076.155726/1‑150 (MQ=60)
                                            CATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAC                                                                                                      >  SRR19973076.1907810/1‑150 (MQ=60)
                                             ATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACATCCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGAGCAAAAACACCAGCCAGGGTAAACC                                                                                                     <  SRR19973076.4442931/150‑1 (MQ=60)
                                              TCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCT                                                                                                    <  SRR19973076.3418157/150‑1 (MQ=60)
                                              TCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCT                                                                                                    <  SRR19973076.4149734/150‑1 (MQ=60)
                                              TCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCT                                                                                                    <  SRR19973076.4171889/150‑1 (MQ=60)
                                              TCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCT                                                                                                    >  SRR19973076.3086323/1‑150 (MQ=60)
                                              TCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCT                                                                                                    >  SRR19973076.3086322/1‑150 (MQ=60)
                                                 AATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGT                                                                                                 >  SRR19973076.3832029/1‑150 (MQ=60)
                                                    CCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTT                                                                                              <  SRR19973076.2646079/150‑1 (MQ=60)
                                                    CCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTT                                                                                              <  SRR19973076.2646092/150‑1 (MQ=60)
                                                     CATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTC                                                                                             <  SRR19973076.250148/150‑1 (MQ=60)
                                                     CATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTC                                                                                             <  SRR19973076.250188/150‑1 (MQ=60)
                                                      ATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCC                                                                                            <  SRR19973076.3038516/150‑1 (MQ=60)
                                                      ATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCC                                                                                            >  SRR19973076.2611953/1‑150 (MQ=60)
                                                              TAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTG                                                                                    >  SRR19973076.3949960/1‑150 (MQ=60)
                                                                            CCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGG                                                                      >  SRR19973076.197854/1‑150 (MQ=60)
                                                                                   GACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAA                                                               <  SRR19973076.3165364/150‑1 (MQ=60)
                                                                                   GACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAA                                                               <  SRR19973076.3192979/150‑1 (MQ=60)
                                                                                        GGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGC                                                          >  SRR19973076.4148009/1‑150 (MQ=60)
                                                                                           AGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTA                                                       <  SRR19973076.4527195/150‑1 (MQ=60)
                                                                                           AGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTA                                                       >  SRR19973076.3772901/1‑150 (MQ=60)
                                                                                           AGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTA                                                       <  SRR19973076.4526438/150‑1 (MQ=60)
                                                                                            GATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTAC                                                      >  SRR19973076.3013189/1‑150 (MQ=60)
                                                                                            GATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCAAAAATGGCTTAC                                                      >  SRR19973076.3013182/1‑150 (MQ=60)
                                                                                              TCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCT                                                    <  SRR19973076.188761/150‑1 (MQ=60)
                                                                                               CAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTG                                                   >  SRR19973076.433945/1‑150 (MQ=60)
                                                                                               CAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTG                                                   <  SRR19973076.1466615/150‑1 (MQ=60)
                                                                                               CAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTG                                                   <  SRR19973076.1466605/150‑1 (MQ=60)
                                                                                                AACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGC                                                  <  SRR19973076.2087146/150‑1 (MQ=60)
                                                                                                 ACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCG                                                 <  SRR19973076.1416709/150‑1 (MQ=60)
                                                                                                      CAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGA                                            >  SRR19973076.3555183/1‑150 (MQ=60)
                                                                                                      CAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGA                                            >  SRR19973076.3556321/1‑150 (MQ=60)
                                                                                                       AACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAA                                           >  SRR19973076.2195209/1‑150 (MQ=60)
                                                                                                             GAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCT                                     <  SRR19973076.4298420/150‑1 (MQ=60)
                                                                                                             GAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCT                                     <  SRR19973076.4298464/150‑1 (MQ=60)
                                                                                                             GAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCT                                     <  SRR19973076.4007483/150‑1 (MQ=60)
                                                                                                             GAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCT                                     <  SRR19973076.4007519/150‑1 (MQ=60)
                                                                                                              AGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTG                                    >  SRR19973076.3197819/1‑150 (MQ=60)
                                                                                                              AGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTG                                    >  SRR19973076.468593/1‑150 (MQ=60)
                                                                                                              AGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTG                                    >  SRR19973076.467606/1‑150 (MQ=60)
                                                                                                                TAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGA                                  >  SRR19973076.377462/1‑150 (MQ=60)
                                                                                                                TAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGA                                  <  SRR19973076.38809/150‑1 (MQ=60)
                                                                                                                  GTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGC                                <  SRR19973076.518233/150‑1 (MQ=60)
                                                                                                                  GTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGC                                <  SRR19973076.517316/150‑1 (MQ=60)
                                                                                                                      GCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTT                            <  SRR19973076.1742356/150‑1 (MQ=60)
                                                                                                                        AAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGC                          >  SRR19973076.1816125/1‑150 (MQ=60)
                                                                                                                         AACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGGTTGCG                         >  SRR19973076.4200764/1‑150 (MQ=60)
                                                                                                                          ACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGC                        >  SRR19973076.1348793/1‑150 (MQ=60)
                                                                                                                            GCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCAC                      <  SRR19973076.3574410/150‑1 (MQ=60)
                                                                                                                            GCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCAC                      <  SRR19973076.352028/150‑1 (MQ=60)
                                                                                                                              CTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCA                    >  SRR19973076.2804085/1‑150 (MQ=60)
                                                                                                                                CGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATC                  >  SRR19973076.3434015/1‑150 (MQ=60)
                                                                                                                                CGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATC                  <  SRR19973076.4400718/150‑1 (MQ=60)
                                                                                                                                CGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATC                  >  SRR19973076.3434052/1‑150 (MQ=60)
                                                                                                                                  CCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGG                <  SRR19973076.597316/150‑1 (MQ=60)
                                                                                                                                    CACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTG              >  SRR19973076.1365897/1‑150 (MQ=60)
                                                                                                                                    CACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTG              >  SRR19973076.1366765/1‑150 (MQ=60)
                                                                                                                                           TGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAA       <  SRR19973076.2049414/150‑1 (MQ=60)
                                                                                                                                           TGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAA       <  SRR19973076.4423324/150‑1 (MQ=60)
                                                                                                                                           TGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAA       <  SRR19973076.4423162/150‑1 (MQ=60)
                                                                                                                                            GCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAAC      >  SRR19973076.4536615/1‑150 (MQ=60)
                                                                                                                                            GCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAAC      >  SRR19973076.1429887/1‑150 (MQ=60)
                                                                                                                                            GCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAAC      >  SRR19973076.4537687/1‑150 (MQ=60)
                                                                                                                                            GCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAAC      >  SRR19973076.4536558/1‑150 (MQ=60)
                                                                                                                                              CATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCA    <  SRR19973076.1059411/150‑1 (MQ=60)
                                                                                                                                                TTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACC  >  SRR19973076.2676389/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                   
GATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACC  >  CP009273/2370841‑2371134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑