Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,767,000 G→T 65.7% D454Y (GAC→TAC)  mtlA → fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,767,0000GT65.7% 199.5 / 309.5 254D454Y (GAC→TAC) mtlAfused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components
Reads supporting (aligned to +/- strand):  ref base G (40/47);  new base T (78/89);  total (118/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 256 total aligned reads displayed.

CAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCTTCCTC  >  CP009273/3766854‑3767149
                                                                                                                                                  |                                                                                                                                                     
cAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACa                                                                                                                                                    >  1:1770052/1‑150 (MQ=255)
     cTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGc                                                                                                                                               <  2:1471203/150‑1 (MQ=255)
      tGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGcc                                                                                                                                              <  1:3226524/150‑1 (MQ=255)
      tGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGcc                                                                                                                                              <  1:2638131/150‑1 (MQ=255)
       gTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCt                                                                                                                                             <  1:521900/150‑1 (MQ=255)
            aGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTaca                                                                                                                                        >  2:4411780/1‑150 (MQ=255)
              aTTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACAcc                                                                                                                                      >  1:3555086/1‑150 (MQ=255)
              aTTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACAcc                                                                                                                                      <  2:1227928/150‑1 (MQ=255)
               tttCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCa                                                                                                                                     >  2:3938832/1‑150 (MQ=255)
                ttCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAg                                                                                                                                    <  1:827962/150‑1 (MQ=255)
                ttCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAg                                                                                                                                    <  1:3938362/150‑1 (MQ=255)
                ttCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAg                                                                                                                                    <  2:828714/150‑1 (MQ=255)
                ttCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAg                                                                                                                                    >  2:493689/1‑150 (MQ=255)
                 tCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGc                                                                                                                                   >  2:2140144/1‑150 (MQ=255)
                 tCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGc                                                                                                                                   <  2:1964697/150‑1 (MQ=255)
                  cTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGcc                                                                                                                                  >  2:4428259/1‑150 (MQ=255)
                  cTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGcc                                                                                                                                  >  2:4181248/1‑150 (MQ=255)
                  cTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGcc                                                                                                                                  >  2:2705936/1‑150 (MQ=255)
                  cTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGcc                                                                                                                                  >  2:1580841/1‑150 (MQ=255)
                   tGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCt                                                                                                                                 >  1:1128034/1‑150 (MQ=255)
                          aaCAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGaa                                                                                                                          >  2:632661/1‑150 (MQ=255)
                          aaCAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGaa                                                                                                                          >  2:634558/1‑150 (MQ=255)
                           aCAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAAc                                                                                                                         <  2:4350255/150‑1 (MQ=255)
                           aCAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAAc                                                                                                                         <  1:1024995/150‑1 (MQ=255)
                            cAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACg                                                                                                                        >  2:3722471/1‑150 (MQ=255)
                             aGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGt                                                                                                                       >  1:286832/1‑150 (MQ=255)
                                gcgATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTg                                                                                                                    >  2:3461060/1‑150 (MQ=255)
                                  gATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGt                                                                                                                  <  1:1987124/150‑1 (MQ=255)
                                  gATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGt                                                                                                                  >  1:1944074/1‑150 (MQ=255)
                                   aTCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGtt                                                                                                                 <  2:2080694/150‑1 (MQ=255)
                                    tCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTtg                                                                                                                >  2:740220/1‑150 (MQ=255)
                                      aacaacCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGGCCGAACGTCTGGTtgct                                                                                                              <  1:1674662/150‑1 (MQ=255)
                                      aacaacCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTtgct                                                                                                              <  1:1155445/150‑1 (MQ=255)
                                      aacaacCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTtgct                                                                                                              <  1:1155397/150‑1 (MQ=255)
                                         aacCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGcc                                                                                                           >  2:2048676/1‑150 (MQ=255)
                                            cTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCaa                                                                                                        >  2:1017615/1‑150 (MQ=255)
                                             tGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTGCTCTAAAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAAc                                                                                                       <  1:2931246/150‑1 (MQ=255)
                                             tGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAAc                                                                                                       <  1:1184392/150‑1 (MQ=255)
                                              gccgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACg                                                                                                      <  1:1177082/150‑1 (MQ=255)
                                                cgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGcc                                                                                                    >  1:1230004/1‑150 (MQ=255)
                                                  ccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCcac                                                                                                  >  1:593727/1‑150 (MQ=255)
                                                    aGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCacac                                                                                                >  1:4123838/1‑150 (MQ=255)
                                                          ggACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCaaa                                                                                          <  1:1033059/150‑1 (MQ=255)
                                                            aCCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACg                                                                                        <  1:2481965/150‑1 (MQ=255)
                                                                 gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAagag                                                                                   >  1:2595923/1‑150 (MQ=255)
                                                                 gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAagag                                                                                   >  1:3217007/1‑150 (MQ=255)
                                                                 gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAagag                                                                                   <  1:4184321/150‑1 (MQ=255)
                                                                  tcatcaCTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAgaga                                                                                  <  2:3008482/150‑1 (MQ=255)
                                                                   catcaCTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGaa                                                                                 <  1:208484/150‑1 (MQ=255)
                                                                   catcaCTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGaa                                                                                 <  1:4461223/150‑1 (MQ=255)
                                                                    atcaCTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGaaa                                                                                <  1:1660909/150‑1 (MQ=255)
                                                                    atcaCTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGaaa                                                                                <  1:1661166/150‑1 (MQ=255)
                                                                    atcaCTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGaaa                                                                                <  2:650840/150‑1 (MQ=255)
                                                                     tcactcacCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAg                                                                               <  1:1081629/150‑1 (MQ=255)
                                                                      cactcacCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGt                                                                              <  2:1642216/150‑1 (MQ=255)
                                                                        ctcacCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTaa                                                                            <  2:231653/150‑1 (MQ=255)
                                                                        ctcacCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTaa                                                                            >  2:3958978/1‑150 (MQ=255)
                                                                                gacctgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGc                                                                    >  2:3265158/1‑150 (MQ=255)
                                                                                  cctgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCt                                                                  <  1:258746/150‑1 (MQ=255)
                                                                                   ctgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTg                                                                 <  2:4539308/150‑1 (MQ=255)
                                                                                     gaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGaa                                                               <  1:2207323/150‑1 (MQ=255)
                                                                                     gaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGaa                                                               <  1:2563311/150‑1 (MQ=255)
                                                                                        cGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGa                                                            >  1:337865/1‑150 (MQ=255)
                                                                                        cGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACGTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGa                                                            >  1:161327/1‑150 (MQ=255)
                                                                                          aaCGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTTACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACa                                                          >  1:4443967/1‑150 (MQ=255)
                                                                                          aaCGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACa                                                          >  2:358353/1‑150 (MQ=255)
                                                                                            cgcgCTATGCTCCAGGTTCCGCAGTCACAGCATATTTCGCTGACCAACTTCCTCTTCAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGATAGTAAAAGACAGCCTGAAAGACAGc                                                        <  2:3733777/150‑1 (MQ=255)
                                                                                            cgcgCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGc                                                        <  1:1863603/150‑1 (MQ=255)
                                                                                            cgcgCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGc                                                        <  1:314287/150‑1 (MQ=255)
                                                                                             gcgcTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCt                                                       <  1:3113846/150‑1 (MQ=255)
                                                                                              cgcTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCtt                                                      >  2:1404642/1‑150 (MQ=255)
                                                                                              cgcTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCtt                                                      <  1:875270/150‑1 (MQ=255)
                                                                                               gcTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCttt                                                     <  2:4011410/150‑1 (MQ=255)
                                                                                                  aTGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGAc                                                  <  2:2631956/150‑1 (MQ=255)
                                                                                                   tGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACg                                                 >  1:1760916/1‑150 (MQ=255)
                                                                                                   tGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACg                                                 <  2:1351863/150‑1 (MQ=255)
                                                                                                        cAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTcc                                            <  1:689865/150‑1 (MQ=255)
                                                                                                        cAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTcc                                            <  2:4128863/150‑1 (MQ=255)
                                                                                                           gTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGt                                         <  2:3807101/150‑1 (MQ=255)
                                                                                                            ttCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTg                                        <  2:1884391/150‑1 (MQ=255)
                                                                                                               cGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTa                                     <  1:2388574/150‑1 (MQ=255)
                                                                                                               cGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTa                                     <  1:85322/150‑1 (MQ=255)
                                                                                                               cGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTa                                     >  2:2590503/1‑150 (MQ=255)
                                                                                                                 cAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAAc                                   >  2:2594723/1‑150 (MQ=255)
                                                                                                                   ggCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCt                                 >  1:1175817/1‑150 (MQ=255)
                                                                                                                   ggCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCt                                 >  1:1175964/1‑150 (MQ=255)
                                                                                                                       caGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTc                             >  2:2836123/1‑150 (MQ=255)
                                                                                                                          cATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAg                          <  1:455204/150‑1 (MQ=255)
                                                                                                                                 cGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGgcg                   >  2:3039666/1‑150 (MQ=255)
                                                                                                                                   cTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGcgcg                 >  2:908617/1‑150 (MQ=255)
                                                                                                                                   cTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGcgcg                 >  2:3413408/1‑150 (MQ=255)
                                                                                                                                            ttCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATc        <  1:2425650/150‑1 (MQ=255)
                                                                                                                                            ttCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATc        >  2:3264730/1‑150 (MQ=255)
                                                                                                                                            ttCCTCTACAGAGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATc        >  2:3625842/1‑150 (MQ=255)
                                                                                                                                             tCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCt       <  2:1847200/150‑1 (MQ=255)
                                                                                                                                              ccTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTTCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCtt      <  2:2458214/150‑1 (MQ=255)
                                                                                                                                              ccTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCtt      <  2:3148887/150‑1 (MQ=255)
                                                                                                                                              ccTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCtt      <  2:2456836/150‑1 (MQ=255)
                                                                                                                                               cTCGCCAGCGGCCTGTACACCAGCCTGACCGACCGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGACAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCTTc     <  2:2914075/150‑1 (MQ=255)
                                                                                                                                               cTCGACAGCGGCCTGTACACCAGCCTGATCGAACGTCTGGTTGCTGCCCAACTCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACTGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTTGGCGCGGAGAACATCTTc     <  2:2914012/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCTTCCTC  >  CP009273/3766854‑3767149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCTTCC  >  CP009273/3766854‑3767147
                                                                                                                                                  |                                                                                                                                                   
CAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACA                                                                                                                                                  >  SRR19973076.1772608/1‑150 (MQ=60)
CAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACA                                                                                                                                                  >  SRR19973076.3518406/1‑150 (MQ=60)
CAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACA                                                                                                                                                  >  SRR19973076.3518349/1‑150 (MQ=60)
     CTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGC                                                                                                                                             >  SRR19973076.1667867/1‑150 (MQ=60)
      TGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCC                                                                                                                                            <  SRR19973076.2641974/150‑1 (MQ=60)
      TGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCC                                                                                                                                            <  SRR19973076.3231213/150‑1 (MQ=60)
       GTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCT                                                                                                                                           >  SRR19973076.2904991/1‑150 (MQ=60)
       GTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCT                                                                                                                                           <  SRR19973076.522734/150‑1 (MQ=60)
       GTCGAAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCT                                                                                                                                           <  SRR19973076.522754/150‑1 (MQ=60)
              ATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACC                                                                                                                                    >  SRR19973076.3560254/1‑150 (MQ=60)
                TTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAG                                                                                                                                  <  SRR19973076.829177/150‑1 (MQ=60)
                TTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAG                                                                                                                                  <  SRR19973076.3944175/150‑1 (MQ=60)
                   TGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCT                                                                                                                               >  SRR19973076.1129697/1‑150 (MQ=60)
                   TGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCT                                                                                                                               >  SRR19973076.1129698/1‑150 (MQ=60)
                   TGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCT                                                                                                                               >  SRR19973076.4531351/1‑150 (MQ=60)
                           ACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAAC                                                                                                                       <  SRR19973076.1026521/150‑1 (MQ=60)
                             AGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGT                                                                                                                     >  SRR19973076.287293/1‑150 (MQ=60)
                                 CGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGG                                                                                                                 >  SRR19973076.2250211/1‑150 (MQ=60)
                                  GATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGT                                                                                                                <  SRR19973076.1990007/150‑1 (MQ=60)
                                  GATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGT                                                                                                                <  SRR19973076.1990006/150‑1 (MQ=60)
                                  GATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGT                                                                                                                >  SRR19973076.4134964/1‑150 (MQ=60)
                                  GATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGT                                                                                                                >  SRR19973076.1946898/1‑150 (MQ=60)
                                  GATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGT                                                                                                                >  SRR19973076.1946908/1‑150 (MQ=60)
                                      AACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGGCCGAACGTCTGGTTGCT                                                                                                            <  SRR19973076.1677109/150‑1 (MQ=60)
                                      AACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCT                                                                                                            <  SRR19973076.3944329/150‑1 (MQ=60)
                                      AACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCT                                                                                                            <  SRR19973076.1157159/150‑1 (MQ=60)
                                      AACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCT                                                                                                            <  SRR19973076.1157153/150‑1 (MQ=60)
                                      AACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCT                                                                                                            <  SRR19973076.1157133/150‑1 (MQ=60)
                                      AACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCT                                                                                                            <  SRR19973076.1157105/150‑1 (MQ=60)
                                      AACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCT                                                                                                            <  SRR19973076.1156551/150‑1 (MQ=60)
                                             TGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTGCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAAC                                                                                                     <  SRR19973076.2935560/150‑1 (MQ=60)
                                             TGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTGCTCTAAAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAAC                                                                                                     <  SRR19973076.2935502/150‑1 (MQ=60)
                                             TGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAAC                                                                                                     <  SRR19973076.1186153/150‑1 (MQ=60)
                                             TGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAAC                                                                                                     <  SRR19973076.1186182/150‑1 (MQ=60)
                                              GCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACG                                                                                                    <  SRR19973076.1178827/150‑1 (MQ=60)
                                              GCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACG                                                                                                    <  SRR19973076.1178872/150‑1 (MQ=60)
                                              GCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACG                                                                                                    <  SRR19973076.1178817/150‑1 (MQ=60)
                                                CGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCC                                                                                                  >  SRR19973076.1231826/1‑150 (MQ=60)
                                                CGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCC                                                                                                  >  SRR19973076.1231848/1‑150 (MQ=60)
                                                  CCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCAC                                                                                                >  SRR19973076.594645/1‑150 (MQ=60)
                                                    AGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACAC                                                                                              >  SRR19973076.4129929/1‑150 (MQ=60)
                                                    AGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACAC                                                                                              >  SRR19973076.2188693/1‑150 (MQ=60)
                                                    AGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACAC                                                                                              <  SRR19973076.1801302/150‑1 (MQ=60)
                                                    AGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACAC                                                                                              <  SRR19973076.1802439/150‑1 (MQ=60)
                                                         TGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAA                                                                                         >  SRR19973076.1082776/1‑150 (MQ=60)
                                                          GGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAA                                                                                        <  SRR19973076.1034594/150‑1 (MQ=60)
                                                          GGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAA                                                                                        <  SRR19973076.1033051/150‑1 (MQ=60)
                                                            ACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACG                                                                                      <  SRR19973076.2485576/150‑1 (MQ=60)
                                                            ACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACG                                                                                      <  SRR19973076.2485612/150‑1 (MQ=60)
                                                            ACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACG                                                                                      <  SRR19973076.2485558/150‑1 (MQ=60)
                                                                 GTCATCACTCACCGTGACCTGACCGTACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAG                                                                                 >  SRR19973076.2599472/1‑150 (MQ=60)
                                                                 GTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAG                                                                                 >  SRR19973076.3222276/1‑150 (MQ=60)
                                                                 GTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAG                                                                                 >  SRR19973076.2599436/1‑150 (MQ=60)
                                                                 GTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAG                                                                                 >  SRR19973076.2599703/1‑150 (MQ=60)
                                                                 GTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAG                                                                                 >  SRR19973076.3221681/1‑150 (MQ=60)
                                                                 GTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAG                                                                                 >  SRR19973076.3221732/1‑150 (MQ=60)
                                                                 GTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAG                                                                                 <  SRR19973076.4190496/150‑1 (MQ=60)
                                                                   CATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAA                                                                               <  SRR19973076.208814/150‑1 (MQ=60)
                                                                   CATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAA                                                                               <  SRR19973076.208836/150‑1 (MQ=60)
                                                                   CATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAA                                                                               <  SRR19973076.4466852/150‑1 (MQ=60)
                                                                   CATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAA                                                                               <  SRR19973076.1913940/150‑1 (MQ=60)
                                                                   CATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAA                                                                               <  SRR19973076.4467865/150‑1 (MQ=60)
                                                                    ATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAA                                                                              <  SRR19973076.1663332/150‑1 (MQ=60)
                                                                    ATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAA                                                                              <  SRR19973076.1663337/150‑1 (MQ=60)
                                                                    ATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAA                                                                              <  SRR19973076.1663338/150‑1 (MQ=60)
                                                                    ATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAA                                                                              <  SRR19973076.1663589/150‑1 (MQ=60)
                                                                    ATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAA                                                                              >  SRR19973076.852234/1‑150 (MQ=60)
                                                                     TCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAG                                                                             <  SRR19973076.1084681/150‑1 (MQ=60)
                                                                     TCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAG                                                                             <  SRR19973076.1083243/150‑1 (MQ=60)
                                                                     TCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAG                                                                             <  SRR19973076.1740389/150‑1 (MQ=60)
                                                                             CGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGAC                                                                     >  SRR19973076.764474/1‑150 (MQ=60)
                                                                               TGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAG                                                                   >  SRR19973076.1924024/1‑150 (MQ=60)
                                                                               TGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAG                                                                   <  SRR19973076.2405256/150‑1 (MQ=60)
                                                                               TGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAG                                                                   <  SRR19973076.4067866/150‑1 (MQ=60)
                                                                                  CCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCT                                                                <  SRR19973076.259175/150‑1 (MQ=60)
                                                                                  CCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCT                                                                <  SRR19973076.258112/150‑1 (MQ=60)
                                                                                    TGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGA                                                              <  SRR19973076.1049342/150‑1 (MQ=60)
                                                                                    TGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGA                                                              <  SRR19973076.494482/150‑1 (MQ=60)
                                                                                     GACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAA                                                             <  SRR19973076.2567043/150‑1 (MQ=60)
                                                                                     GACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAA                                                             <  SRR19973076.2210508/150‑1 (MQ=60)
                                                                                      ACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAA                                                            >  SRR19973076.272542/1‑150 (MQ=60)
                                                                                        CGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGA                                                          >  SRR19973076.161738/1‑150 (MQ=60)
                                                                                        CGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGA                                                          >  SRR19973076.338387/1‑150 (MQ=60)
                                                                                        CGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACGTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGA                                                          >  SRR19973076.161604/1‑150 (MQ=60)
                                                                                          AACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTTACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACA                                                        >  SRR19973076.4450582/1‑150 (MQ=60)
                                                                                          AACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACA                                                        >  SRR19973076.916866/1‑150 (MQ=60)
                                                                                            CGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGC                                                      <  SRR19973076.1866297/150‑1 (MQ=60)
                                                                                            CGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGC                                                      <  SRR19973076.314796/150‑1 (MQ=60)
                                                                                            CGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGC                                                      <  SRR19973076.314790/150‑1 (MQ=60)
                                                                                            CGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGC                                                      <  SRR19973076.314788/150‑1 (MQ=60)
                                                                                            CGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGC                                                      <  SRR19973076.68592/150‑1 (MQ=60)
                                                                                             GCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCT                                                     <  SRR19973076.3118406/150‑1 (MQ=60)
                                                                                             GCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCT                                                     <  SRR19973076.3118367/150‑1 (MQ=60)
                                                                                              CGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTT                                                    >  SRR19973076.2634044/1‑150 (MQ=60)
                                                                                              CGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTT                                                    <  SRR19973076.876504/150‑1 (MQ=60)
                                                                                              CGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTT                                                    <  SRR19973076.876563/150‑1 (MQ=60)
                                                                                               GCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTT                                                   <  SRR19973076.2984397/150‑1 (MQ=60)
                                                                                                CTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTG                                                  <  SRR19973076.3664189/150‑1 (MQ=60)
                                                                                                  ATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGAC                                                <  SRR19973076.1182700/150‑1 (MQ=60)
                                                                                                   TGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACG                                               >  SRR19973076.1763463/1‑150 (MQ=60)
                                                                                                   TGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACG                                               >  SRR19973076.1763584/1‑150 (MQ=60)
                                                                                                      GCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATT                                            >  SRR19973076.1161275/1‑150 (MQ=60)
                                                                                                      GCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATT                                            >  SRR19973076.2454765/1‑150 (MQ=60)
                                                                                                        CAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCC                                          <  SRR19973076.690901/150‑1 (MQ=60)
                                                                                                              CCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCT                                    >  SRR19973076.458908/1‑150 (MQ=60)
                                                                                                               CGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTA                                   <  SRR19973076.85500/150‑1 (MQ=60)
                                                                                                               CGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTA                                   <  SRR19973076.85475/150‑1 (MQ=60)
                                                                                                               CGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTA                                   <  SRR19973076.1524281/150‑1 (MQ=60)
                                                                                                               CGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTA                                   <  SRR19973076.2392017/150‑1 (MQ=60)
                                                                                                                   GGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCT                               >  SRR19973076.1177707/1‑150 (MQ=60)
                                                                                                                   GGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCT                               >  SRR19973076.1177708/1‑150 (MQ=60)
                                                                                                                   GGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCT                               >  SRR19973076.1177599/1‑150 (MQ=60)
                                                                                                                   GGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCT                               >  SRR19973076.1177560/1‑150 (MQ=60)
                                                                                                                    GCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTG                              >  SRR19973076.422311/1‑150 (MQ=60)
                                                                                                                          CATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAG                        <  SRR19973076.455939/150‑1 (MQ=60)
                                                                                                                          CATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAG                        <  SRR19973076.455836/150‑1 (MQ=60)
                                                                                                                          CATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAG                        <  SRR19973076.4289322/150‑1 (MQ=60)
                                                                                                                                TCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGC                  <  SRR19973076.2491842/150‑1 (MQ=60)
                                                                                                                                TCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGC                  >  SRR19973076.328049/1‑150 (MQ=60)
                                                                                                                                          ACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACA        <  SRR19973076.2388245/150‑1 (MQ=60)
                                                                                                                                           CTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACAT       >  SRR19973076.623454/1‑150 (MQ=60)
                                                                                                                                           CTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACAT       >  SRR19973076.2861687/1‑150 (MQ=60)
                                                                                                                                            TTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATC      <  SRR19973076.2429150/150‑1 (MQ=60)
                                                                                                                                                TCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCTTCC  >  SRR19973076.1752629/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                   
CAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCTTCC  >  CP009273/3766854‑3767147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑