Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,218,928 G→A 12.1% E424K (GAA→AAA)  yjbB → putative Na+/Pi‑cotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,218,9280GA12.1% 744.7 / 73.8 248E424K (GAA→AAA) yjbBputative Na+/Pi‑cotransporter
Reads supporting (aligned to +/- strand):  ref base G (91/126);  new base A (15/15);  total (106/142)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

CAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTG  >  CP009273/4218780‑4219077
                                                                                                                                                    |                                                                                                                                                     
cAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGa                                                                                                                                                      <  2:3492185/150‑1 (MQ=255)
    gTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAAtt                                                                                                                                                  <  2:1285011/150‑1 (MQ=255)
     ttCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTg                                                                                                                                                 >  1:691569/1‑150 (MQ=255)
     ttCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTg                                                                                                                                                 >  1:691733/1‑150 (MQ=255)
      tctcTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGc                                                                                                                                                >  1:1345091/1‑150 (MQ=255)
          tACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGAc                                                                                                                                            >  1:2613670/1‑150 (MQ=255)
          tACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGAc                                                                                                                                            <  2:3634168/150‑1 (MQ=255)
          tACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGAc                                                                                                                                            <  1:785776/150‑1 (MQ=255)
           acacCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACa                                                                                                                                           >  1:1323604/1‑150 (MQ=255)
                  cATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACt                                                                                                                                    >  2:4075930/1‑150 (MQ=255)
                     tAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGGGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGgc                                                                                                                                 <  1:3959701/150‑1 (MQ=255)
                     tAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGgc                                                                                                                                 <  2:3067904/150‑1 (MQ=255)
                     tAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGgc                                                                                                                                 >  2:1490439/1‑150 (MQ=255)
                     tAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGgc                                                                                                                                 >  2:2222270/1‑150 (MQ=255)
                      aaGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGgca                                                                                                                                >  2:2192567/1‑150 (MQ=255)
                       aGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGgcag                                                                                                                               <  2:1752326/150‑1 (MQ=255)
                            tATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCAcgg                                                                                                                          <  2:2474661/150‑1 (MQ=255)
                             aTCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCAcggc                                                                                                                         >  1:158757/1‑150 (MQ=255)
                               cTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCAcggcgg                                                                                                                       <  1:3590783/150‑1 (MQ=255)
                                tGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCggg                                                                                                                      >  2:1410945/1‑150 (MQ=255)
                                  gcgcGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCa                                                                                                                    <  2:1877386/150‑1 (MQ=255)
                                  gcgcGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCa                                                                                                                    <  2:3796989/150‑1 (MQ=255)
                                   cgcgGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCAt                                                                                                                   >  2:684237/1‑150 (MQ=255)
                                    gcgGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCAtt                                                                                                                  <  2:1572069/150‑1 (MQ=255)
                                     cgGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCAttt                                                                                                                 >  1:1109630/1‑150 (MQ=255)
                                     cgGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCAttt                                                                                                                 >  1:1109536/1‑150 (MQ=255)
                                      ggATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCAtttt                                                                                                                >  1:2734700/1‑150 (MQ=255)
                                         tGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGc                                                                                                             <  1:1384310/150‑1 (MQ=255)
                                           ccAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCtt                                                                                                           >  1:3863879/1‑150 (MQ=255)
                                            cAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGCTCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTg                                                                                                          >  2:4238984/1‑150 (MQ=255)
                                              aaaGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGAt                                                                                                        >  2:1221589/1‑150 (MQ=255)
                                              aaaGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGAt                                                                                                        <  2:4071471/150‑1 (MQ=255)
                                              aaaGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGAt                                                                                                        <  2:4071123/150‑1 (MQ=255)
                                                  aagaGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGt                                                                                                    <  1:1011297/150‑1 (MQ=255)
                                                        tGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAgg                                                                                              <  1:2219928/150‑1 (MQ=255)
                                                        tGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAgg                                                                                              <  1:502043/150‑1 (MQ=255)
                                                         ggCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGa                                                                                             <  2:1264248/150‑1 (MQ=255)
                                                         ggCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGa                                                                                             <  2:3577889/150‑1 (MQ=255)
                                                         ggCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGa                                                                                             <  2:3267122/150‑1 (MQ=255)
                                                           cGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGATCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATTGGCCGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAAc                                                                                           >  1:2932333/1‑150 (MQ=255)
                                                            ggaggaAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACt                                                                                          <  1:3251352/150‑1 (MQ=255)
                                                               ggaAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGa                                                                                       <  2:3040482/150‑1 (MQ=255)
                                                                gaAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGAt                                                                                      <  1:4272578/150‑1 (MQ=255)
                                                                 aaGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATg                                                                                     >  1:3489852/1‑150 (MQ=255)
                                                                 aaGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATg                                                                                     >  1:2712480/1‑150 (MQ=255)
                                                                   gagTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATgcg                                                                                   <  1:783853/150‑1 (MQ=255)
                                                                      tCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGctc                                                                                >  1:1720772/1‑150 (MQ=255)
                                                                        gcgcCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTa                                                                              <  2:2492110/150‑1 (MQ=255)
                                                                         cgccgcTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTAt                                                                             <  1:2056314/150‑1 (MQ=255)
                                                                                 ggCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCTAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGGTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAAtt                                                                     <  1:3692551/150‑1 (MQ=255)
                                                                                   cAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGc                                                                   <  1:1486253/150‑1 (MQ=255)
                                                                                     gagaTCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTc                                                                 <  1:880892/150‑1 (MQ=255)
                                                                                       gaTCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAg                                                               >  2:3990391/1‑150 (MQ=255)
                                                                                       gaTCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAg                                                               <  2:1516053/150‑1 (MQ=255)
                                                                                        atcatcGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGt                                                              <  2:2813433/150‑1 (MQ=255)
                                                                                           atcGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCCGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAAt                                                           >  1:1803473/1‑150 (MQ=255)
                                                                                            tcGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAAtt                                                          <  2:4320431/150‑1 (MQ=255)
                                                                                            tcGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAAtt                                                          <  2:1208462/150‑1 (MQ=255)
                                                                                            tcGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAAtt                                                          <  2:2073464/150‑1 (MQ=255)
                                                                                              gAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTa                                                        <  1:3944532/150‑1 (MQ=255)
                                                                                              gAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTa                                                        >  1:2594783/1‑150 (MQ=255)
                                                                                               aaaTGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTaa                                                       <  2:3654365/150‑1 (MQ=255)
                                                                                               aaaTGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTaa                                                       >  1:1290414/1‑150 (MQ=255)
                                                                                                   gTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCt                                                   >  2:564939/1‑150 (MQ=255)
                                                                                                    tCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTg                                                  >  2:845151/1‑150 (MQ=255)
                                                                                                      gCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGc                                                >  2:610764/1‑150 (MQ=255)
                                                                                                         cAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAAt                                             >  1:3564540/1‑150 (MQ=255)
                                                                                                          aaCCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATg                                            >  1:3150856/1‑150 (MQ=255)
                                                                                                          aaCCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATg                                            >  1:2489411/1‑150 (MQ=255)
                                                                                                           aCCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGt                                           <  1:4333744/150‑1 (MQ=255)
                                                                                                             cTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCg                                         >  1:104285/1‑150 (MQ=255)
                                                                                                                 aaCAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGgtgt                                     >  2:4091703/1‑150 (MQ=255)
                                                                                                                 aaCAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGgtgt                                     <  2:1170964/150‑1 (MQ=255)
                                                                                                                 aaCAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGgtgt                                     >  1:289815/1‑150 (MQ=255)
                                                                                                                 aaCAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGgtgt                                     >  1:3216207/1‑150 (MQ=255)
                                                                                                                   cAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGttc                                   <  1:3753769/150‑1 (MQ=255)
                                                                                                                   cAGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGttc                                   >  1:398198/1‑150 (MQ=255)
                                                                                                                    aGGCCTCCGATATCGTCGAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGttct                                  >  1:2093715/1‑150 (MQ=255)
                                                                                                                     ggCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGttctt                                 <  1:2443054/150‑1 (MQ=255)
                                                                                                                       ccTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTtcttct                               <  1:3260664/150‑1 (MQ=255)
                                                                                                                       ccTCCGATATCGTCGAGCGCATGGGCAGCGAAAGTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTtcttct                               <  2:3474500/150‑1 (MQ=255)
                                                                                                                        cTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTtctc                              <  2:2942815/150‑1 (MQ=255)
                                                                                                                        cTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTtctc                              >  2:17268/1‑150 (MQ=255)
                                                                                                                           cGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCAGTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAACGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTgg                           >  1:3701260/1‑150 (MQ=255)
                                                                                                                           cGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTgg                           <  2:1321456/150‑1 (MQ=255)
                                                                                                                            gATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGc                          <  2:47682/150‑1 (MQ=255)
                                                                                                                              tatCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGa                        <  1:4075930/150‑1 (MQ=255)
                                                                                                                                 cgtcgAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGt                     >  2:863287/1‑150 (MQ=255)
                                                                                                                                  gtcgAGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTc                    >  2:4229905/1‑150 (MQ=255)
                                                                                                                                     gAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCAcc                 <  2:3872323/150‑1 (MQ=255)
                                                                                                                                     gAGCGCATGGGCAGCGAAAGTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCAcc                 <  2:3872360/150‑1 (MQ=255)
                                                                                                                                      aGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCa                <  1:19219/150‑1 (MQ=255)
                                                                                                                                      aGCGCATGGGCAGCAAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCa                <  1:893455/150‑1 (MQ=255)
                                                                                                                                         gcATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAgcg             <  2:379751/150‑1 (MQ=255)
                                                                                                                                         gcATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAgcg             <  2:1121459/150‑1 (MQ=255)
                                                                                                                                           aTGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCt           <  2:3170016/150‑1 (MQ=255)
                                                                                                                                                cAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTTATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCtcgtcg      <  2:2952013/150‑1 (MQ=255)
                                                                                                                                                 aGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGTTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTcgtcgt     >  2:4001206/1‑150 (MQ=255)
                                                                                                                                                   cGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGttt   >  1:468321/1‑150 (MQ=255)
                                                                                                                                                    gAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTg  >  2:2794538/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGGAAGAGTCGCGCCGCTGGGCAGAGATCATCGAAATGTCGCTCAACCTTGAACAGGCCTCCGATATCGTCGAGCGCATGGGCAGCGAAATTGCTGACAAATCACTGGCAGCACGGCGGGCATTTTCGCTTGATGGGTTGAAGGAACTGGATGCGCTCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTG  >  CP009273/4218780‑4219077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A