Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,388,406 G→T 85.9% R308L (CGC→CTC)  mppA → murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,388,4060GT85.9% 666.9 / 112.3 256R308L (CGC→CTC) mppAmurein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (20/16);  new base T (120/100);  total (140/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

Only 100 of 256 total aligned reads displayed.

GGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTC  >  CP009273/1388261‑1388552
                                                                                                                                                 |                                                                                                                                                  
 gggATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGACGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTc                                                                                                                                               <  1:991261/150‑1 (MQ=255)
 gggATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTc                                                                                                                                               <  1:3594093/150‑1 (MQ=255)
  ggATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCg                                                                                                                                              <  2:1042222/150‑1 (MQ=255)
   gATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGt                                                                                                                                             <  2:3976622/150‑1 (MQ=255)
     tatTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCt                                                                                                                                           >  2:210560/1‑150 (MQ=255)
     tatTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCt                                                                                                                                           >  2:209469/1‑150 (MQ=255)
           tatCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCAtt                                                                                                                                     >  2:3706046/1‑150 (MQ=255)
            atCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTa                                                                                                                                    >  1:1608337/1‑150 (MQ=255)
              cACCGAATCCTTCCCTAAAAATATGTATCAGAATCTGTTGTATGATATTCCGTGGCAGTTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGACGACGGCAGATCAGCTCGTTCGTCTGGCATTAAg                                                                                                                                  <  1:2025623/150‑1 (MQ=255)
              cACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAg                                                                                                                                  <  1:1313694/150‑1 (MQ=255)
                ccGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTa                                                                                                                                >  1:3942840/1‑150 (MQ=255)
                ccGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTa                                                                                                                                >  1:2747190/1‑150 (MQ=255)
                   aaTCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCAGTAAGTATGa                                                                                                                             >  2:4285204/1‑150 (MQ=255)
                    aTCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGAc                                                                                                                            >  2:1795031/1‑150 (MQ=255)
                     tCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACg                                                                                                                           <  2:521575/150‑1 (MQ=255)
                      ccTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGa                                                                                                                          >  1:85924/1‑150 (MQ=255)
                        ttCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATa                                                                                                                        <  2:2915832/150‑1 (MQ=255)
                          cccGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGa                                                                                                                      >  1:2347700/1‑150 (MQ=255)
                                aaaTATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATcgccg                                                                                                                >  1:1176664/1‑150 (MQ=255)
                                 aaTATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATcgccgc                                                                                                               >  2:4326564/1‑150 (MQ=255)
                                   tatGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCt                                                                                                             <  1:2578384/150‑1 (MQ=255)
                                     tGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCtga                                                                                                           <  1:4462568/150‑1 (MQ=255)
                                      gTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCtgat                                                                                                          <  2:1771291/150‑1 (MQ=255)
                                       tATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCtgatg                                                                                                         >  1:887203/1‑150 (MQ=255)
                                       tATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCtgatg                                                                                                         <  1:1579691/150‑1 (MQ=255)
                                       tATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCtgatg                                                                                                         <  2:1166358/150‑1 (MQ=255)
                                             aaGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGaa                                                                                                   >  2:878408/1‑150 (MQ=255)
                                                cTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGaaaaa                                                                                                >  2:2324913/1‑150 (MQ=255)
                                                 tGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAg                                                                                               >  2:2609084/1‑150 (MQ=255)
                                                 tGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAg                                                                                               >  2:1548187/1‑150 (MQ=255)
                                                 tGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAg                                                                                               <  2:3856201/150‑1 (MQ=255)
                                                 tGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAg                                                                                               <  2:4005908/150‑1 (MQ=255)
                                                     gAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTAtt                                                                                           >  1:2279486/1‑150 (MQ=255)
                                                      aaGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTa                                                                                          >  2:3524846/1‑150 (MQ=255)
                                                        ggATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAgg                                                                                        >  2:3451305/1‑150 (MQ=255)
                                                        ggATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAgg                                                                                        <  2:1608338/150‑1 (MQ=255)
                                                            atTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACg                                                                                    >  1:134352/1‑150 (MQ=255)
                                                            atTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACg                                                                                    >  2:1310764/1‑150 (MQ=255)
                                                             ttCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACgg                                                                                   <  1:3081225/150‑1 (MQ=255)
                                                                      aGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGc                                                                          >  1:4220616/1‑150 (MQ=255)
                                                                        gTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCa                                                                        >  1:3521301/1‑150 (MQ=255)
                                                                        gTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCa                                                                        <  2:379188/150‑1 (MQ=255)
                                                                        gTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCa                                                                        >  1:2106661/1‑150 (MQ=255)
                                                                        gTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCa                                                                        <  1:740294/150‑1 (MQ=255)
                                                                        gTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCa                                                                        <  2:2721380/150‑1 (MQ=255)
                                                                           tataCGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCg                                                                     <  2:753620/150‑1 (MQ=255)
                                                                           tataCGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCg                                                                     >  2:3130472/1‑150 (MQ=255)
                                                                             taCGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTg                                                                   >  2:3381643/1‑150 (MQ=255)
                                                                                gccgccgcAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCa                                                                >  2:460188/1‑150 (MQ=255)
                                                                                 ccgccgcAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCAt                                                               <  1:1027531/150‑1 (MQ=255)
                                                                                     cgcAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTa                                                           >  1:1184377/1‑150 (MQ=255)
                                                                                       cAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACTGCAGATCAGCTCGTTCGTCTGTCATTAAGTATGACGATAGATCGCCGCCAGATGACCGACAAAGTATTAGGGACGGGCGAAAAGCAAGCGTGGCATTTTaca                                                         >  2:4223184/1‑150 (MQ=255)
                                                                                       cAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTaca                                                         <  1:1308613/150‑1 (MQ=255)
                                                                                         gCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACAcc                                                       <  2:2788871/150‑1 (MQ=255)
                                                                                          cTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCa                                                      >  1:3623804/1‑150 (MQ=255)
                                                                                           tCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAg                                                     <  2:58431/150‑1 (MQ=255)
                                                                                             gggACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGAt                                                   >  1:2242852/1‑150 (MQ=255)
                                                                                              ggACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATg                                                  <  2:1279051/150‑1 (MQ=255)
                                                                                               gACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGt                                                 <  1:4129377/150‑1 (MQ=255)
                                                                                                 ccTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTa                                               <  1:4505365/150‑1 (MQ=255)
                                                                                                 ccTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTa                                               >  1:3198386/1‑150 (MQ=255)
                                                                                                    attattatGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACcg                                            <  1:2304029/150‑1 (MQ=255)
                                                                                                    attattatGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACcg                                            <  2:447363/150‑1 (MQ=255)
                                                                                                       attatGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCgg                                         >  1:2855233/1‑150 (MQ=255)
                                                                                                        ttatGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCggg                                        <  2:3339197/150‑1 (MQ=255)
                                                                                                         tatGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGa                                       >  1:1054754/1‑150 (MQ=255)
                                                                                                         tatGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGa                                       >  1:2506823/1‑150 (MQ=255)
                                                                                                           tGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGAtt                                     >  2:3407656/1‑150 (MQ=255)
                                                                                                           tGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGAtt                                     <  1:3907901/150‑1 (MQ=255)
                                                                                                            gCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGAttt                                    <  2:2904977/150‑1 (MQ=255)
                                                                                                            gCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGAttt                                    <  2:4472702/150‑1 (MQ=255)
                                                                                                             cGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTa                                   <  2:2367364/150‑1 (MQ=255)
                                                                                                              gTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTAc                                  <  1:3283642/150‑1 (MQ=255)
                                                                                                                 tAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGcc                               >  2:4117570/1‑150 (MQ=255)
                                                                                                                 tAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGcc                               >  2:1125025/1‑150 (MQ=255)
                                                                                                                 tAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGcc                               >  1:3703933/1‑150 (MQ=255)
                                                                                                                      cGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAAc                          >  2:2066/1‑150 (MQ=255)
                                                                                                                      cGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAAc                          >  1:3893883/1‑150 (MQ=255)
                                                                                                                       gCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAAcc                         >  1:2127065/1‑150 (MQ=255)
                                                                                                                        cAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCt                        <  2:1367563/150‑1 (MQ=255)
                                                                                                                        cAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCt                        <  2:4216651/150‑1 (MQ=255)
                                                                                                                         aaaaaGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCCGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCtt                       >  2:826775/1‑150 (MQ=255)
                                                                                                                         aaaaaGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCtt                       >  1:35438/1‑150 (MQ=255)
                                                                                                                         aaaaaGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCtt                       >  2:1372079/1‑150 (MQ=255)
                                                                                                                          aaaaGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTc                      >  1:1374884/1‑150 (MQ=255)
                                                                                                                          aaaaGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTc                      >  2:702335/1‑150 (MQ=255)
                                                                                                                          aaaaGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTc                      <  1:3371280/150‑1 (MQ=255)
                                                                                                                          aaaaGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTc                      >  2:2984433/1‑150 (MQ=255)
                                                                                                                           aaaGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCg                     <  2:937317/150‑1 (MQ=255)
                                                                                                                                 ccGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGttt               <  1:4113457/150‑1 (MQ=255)
                                                                                                                                  cGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTg              >  1:4078826/1‑150 (MQ=255)
                                                                                                                                  cGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTg              >  1:3316656/1‑150 (MQ=255)
                                                                                                                                   gACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGa             <  1:1415391/150‑1 (MQ=255)
                                                                                                                                     cGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAAc           <  1:2609084/150‑1 (MQ=255)
                                                                                                                                     cGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAAc           <  1:1548187/150‑1 (MQ=255)
                                                                                                                                        cAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACaaa        >  2:2191200/1‑150 (MQ=255)
                                                                                                                                          gATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATg      >  2:3200718/1‑150 (MQ=255)
                                                                                                                                           aTCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGa     >  2:166497/1‑150 (MQ=255)
                                                                                                                                           aTCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGa     <  1:424098/150‑1 (MQ=255)
                                                                                                                                              aGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTc  >  1:426022/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTC  >  CP009273/1388261‑1388552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGG  >  CP009273/1388257‑1388555
                                                                                                                                                     |                                                                                                                                                     
CGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCT                                                                                                                                                       >  SRR19973080.3418420/1‑150 (MQ=60)
CGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCT                                                                                                                                                       >  SRR19973080.3419195/1‑150 (MQ=60)
 GCGGGGGATATTGATATTACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTC                                                                                                                                                      <  SRR19973080.3749472/150‑1 (MQ=60)
 GCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATCCGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTC                                                                                                                                                      <  SRR19973080.1411354/150‑1 (MQ=60)
 GCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTC                                                                                                                                                      <  SRR19973080.3000480/150‑1 (MQ=60)
 GCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTC                                                                                                                                                      <  SRR19973080.1811860/150‑1 (MQ=60)
 GCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTC                                                                                                                                                      <  SRR19973080.3705320/150‑1 (MQ=60)
    GGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTT                                                                                                                                                   >  SRR19973080.4489470/1‑150 (MQ=60)
     GGGATATTGATATCACCTTATCCTTCCAGAAAAATATGTATCATAAGCTGTTGAATGATATTCCGGGTCAGGTTTATACGCCGCCGCATCTCGGGACCTATTTTTATGAGTTTAAAACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTC                                                                                                                                                  <  SRR19973080.577346/150‑1 (MQ=60)
     GGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGACGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTC                                                                                                                                                  <  SRR19973080.997224/150‑1 (MQ=60)
     GGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTC                                                                                                                                                  <  SRR19973080.3613958/150‑1 (MQ=60)
     GGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTC                                                                                                                                                  <  SRR19973080.2269430/150‑1 (MQ=60)
     GGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTC                                                                                                                                                  >  SRR19973080.902194/1‑150 (MQ=60)
     GGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTC                                                                                                                                                  <  SRR19973080.3702728/150‑1 (MQ=60)
          ATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTG                                                                                                                                             >  SRR19973080.2557993/1‑150 (MQ=60)
                ATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTA                                                                                                                                       >  SRR19973080.1617748/1‑150 (MQ=60)
                  CACCGAATCCTTCCCTAAAAATATGTATCAGAATCTGTTGTATGATATTCCGTGGCAGTTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGACGACGGCAGATCAGCTCGTTCGTCTGGCATTAAG                                                                                                                                     <  SRR19973080.2037139/150‑1 (MQ=60)
                  CACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAG                                                                                                                                     <  SRR19973080.2037141/150‑1 (MQ=60)
                  CACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAG                                                                                                                                     <  SRR19973080.1321628/150‑1 (MQ=60)
                  CACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAG                                                                                                                                     <  SRR19973080.1321631/150‑1 (MQ=60)
                   ACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGT                                                                                                                                    <  SRR19973080.3302733/150‑1 (MQ=60)
                   ACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGT                                                                                                                                    <  SRR19973080.3302739/150‑1 (MQ=60)
                    CCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTA                                                                                                                                   >  SRR19973080.3964577/1‑150 (MQ=60)
                    CCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTA                                                                                                                                   >  SRR19973080.2762318/1‑150 (MQ=60)
                     CGAATCCGTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTAT                                                                                                                                  <  SRR19973080.3924990/150‑1 (MQ=60)
                       AATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGA                                                                                                                                >  SRR19973080.2587010/1‑150 (MQ=60)
                        ATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGAC                                                                                                                               >  SRR19973080.2299916/1‑150 (MQ=60)
                        ATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGAC                                                                                                                               >  SRR19973080.1596483/1‑150 (MQ=60)
                        ATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGAC                                                                                                                               >  SRR19973080.1596623/1‑150 (MQ=60)
                          CCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGA                                                                                                                             >  SRR19973080.86729/1‑150 (MQ=60)
                           CTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGAT                                                                                                                            >  SRR19973080.696748/1‑150 (MQ=60)
                              CCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGA                                                                                                                         >  SRR19973080.2360745/1‑150 (MQ=60)
                              CCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGA                                                                                                                         >  SRR19973080.2360741/1‑150 (MQ=60)
                               CCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGAT                                                                                                                        <  SRR19973080.1572195/150‑1 (MQ=60)
                                CGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATC                                                                                                                       >  SRR19973080.14312/1‑150 (MQ=60)
                                   AAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCC                                                                                                                    <  SRR19973080.1339648/150‑1 (MQ=60)
                                    AAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCG                                                                                                                   >  SRR19973080.1183690/1‑150 (MQ=60)
                                       TATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCT                                                                                                                <  SRR19973080.2592446/150‑1 (MQ=60)
                                         TGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGA                                                                                                              <  SRR19973080.4486750/150‑1 (MQ=60)
                                          GTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGAT                                                                                                             <  SRR19973080.2470942/150‑1 (MQ=60)
                                           TATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATG                                                                                                            >  SRR19973080.892581/1‑150 (MQ=60)
                                           TATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATG                                                                                                            <  SRR19973080.1588906/150‑1 (MQ=60)
                                           TATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATG                                                                                                            >  SRR19973080.892641/1‑150 (MQ=60)
                                           TATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATG                                                                                                            >  SRR19973080.892576/1‑150 (MQ=60)
                                           TATCAGAAGATGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATT                                                                                                            <  SRR19973080.3616774/150‑1 (MQ=60)
                                             TCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGAC                                                                                                          <  SRR19973080.3208282/150‑1 (MQ=60)
                                               AGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCG                                                                                                        <  SRR19973080.1368280/150‑1 (MQ=60)
                                               AGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCG                                                                                                        >  SRR19973080.3575157/1‑150 (MQ=60)
                                               AGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCG                                                                                                        <  SRR19973080.3350492/150‑1 (MQ=60)
                                                       TTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTA                                                                                                <  SRR19973080.2911829/150‑1 (MQ=60)
                                                         GAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATT                                                                                              >  SRR19973080.2291995/1‑150 (MQ=60)
                                                            GGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGG                                                                                           >  SRR19973080.346082/1‑150 (MQ=60)
                                                            GGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGG                                                                                           <  SRR19973080.2083394/150‑1 (MQ=60)
                                                            GGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGG                                                                                           >  SRR19973080.2617267/1‑150 (MQ=60)
                                                            GGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGG                                                                                           >  SRR19973080.2133390/1‑150 (MQ=60)
                                                                ATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACG                                                                                       >  SRR19973080.135476/1‑150 (MQ=60)
                                                                 TTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGG                                                                                      <  SRR19973080.3098050/150‑1 (MQ=60)
                                                                   CCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGC                                                                                    <  SRR19973080.3260058/150‑1 (MQ=60)
                                                                     GGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGA                                                                                  >  SRR19973080.2749857/1‑150 (MQ=60)
                                                                        GCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAA                                                                               >  SRR19973080.2316921/1‑150 (MQ=60)
                                                                          AGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGC                                                                             >  SRR19973080.4243581/1‑150 (MQ=60)
                                                                          AGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGC                                                                             >  SRR19973080.4243609/1‑150 (MQ=60)
                                                                            GTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCA                                                                           >  SRR19973080.2118562/1‑150 (MQ=60)
                                                                            GTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCA                                                                           >  SRR19973080.3540761/1‑150 (MQ=60)
                                                                            GTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCA                                                                           <  SRR19973080.744508/150‑1 (MQ=60)
                                                                            GTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCA                                                                           <  SRR19973080.744528/150‑1 (MQ=60)
                                                                             TTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAG                                                                          <  SRR19973080.3968769/150‑1 (MQ=60)
                                                                               TATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCG                                                                        <  SRR19973080.2245030/150‑1 (MQ=60)
                                                                                    GCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCA                                                                   >  SRR19973080.1137436/1‑150 (MQ=60)
                                                                                     CCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCAT                                                                  <  SRR19973080.1033633/150‑1 (MQ=60)
                                                                                       GCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTT                                                                >  SRR19973080.3060101/1‑150 (MQ=60)
                                                                                         CGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTA                                                              >  SRR19973080.1191468/1‑150 (MQ=60)
                                                                                          GCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTAC                                                             >  SRR19973080.1246608/1‑150 (MQ=60)
                                                                                          GCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGACGCCAGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTAC                                                             >  SRR19973080.3544818/1‑150 (MQ=60)
                                                                                           CAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACA                                                            <  SRR19973080.1316524/150‑1 (MQ=60)
                                                                                              CTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCA                                                         >  SRR19973080.3643833/1‑150 (MQ=60)
                                                                                                 GGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGAT                                                      >  SRR19973080.2255152/1‑150 (MQ=60)
                                                                                                   GACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGT                                                    <  SRR19973080.4151750/150‑1 (MQ=60)
                                                                                                    ACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTT                                                   >  SRR19973080.1328762/1‑150 (MQ=60)
                                                                                                     CCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTA                                                  <  SRR19973080.4529692/150‑1 (MQ=60)
                                                                                                     CCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTA                                                  >  SRR19973080.3216039/1‑150 (MQ=60)
                                                                                                        ATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCG                                               <  SRR19973080.2316775/150‑1 (MQ=60)
                                                                                                        ATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCG                                               >  SRR19973080.1550000/1‑150 (MQ=60)
                                                                                                        ATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCG                                               <  SRR19973080.1190011/150‑1 (MQ=60)
                                                                                                        ATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTAGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCG                                               <  SRR19973080.2316515/150‑1 (MQ=60)
                                                                                                           ATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGG                                            >  SRR19973080.2870891/1‑150 (MQ=60)
                                                                                                           ATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGG                                            >  SRR19973080.2870849/1‑150 (MQ=60)
                                                                                                           ATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGG                                            >  SRR19973080.3788426/1‑150 (MQ=60)
                                                                                                             TATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGA                                          >  SRR19973080.1060945/1‑150 (MQ=60)
                                                                                                             TATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGA                                          >  SRR19973080.1060920/1‑150 (MQ=60)
                                                                                                             TATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGA                                          >  SRR19973080.2520648/1‑150 (MQ=60)
                                                                                                             TATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGA                                          >  SRR19973080.3518949/1‑150 (MQ=60)
                                                                                                               TGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATT                                        <  SRR19973080.4204949/150‑1 (MQ=60)
                                                                                                               TGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATT                                        <  SRR19973080.3929465/150‑1 (MQ=60)
                                                                                                                 CGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTA                                      >  SRR19973080.3945516/1‑150 (MQ=60)
                                                                                                                 CGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTA                                      >  SRR19973080.3945522/1‑150 (MQ=60)
                                                                                                                 CGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTA                                      <  SRR19973080.1122824/150‑1 (MQ=60)
                                                                                                                  GTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTAC                                     <  SRR19973080.3301669/150‑1 (MQ=60)
                                                                                                                  GTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTAC                                     <  SRR19973080.49149/150‑1 (MQ=60)
                                                                                                                     TAACACGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCC                                  <  SRR19973080.3748329/150‑1 (MQ=60)
                                                                                                                     TAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCC                                  >  SRR19973080.3724268/1‑150 (MQ=60)
                                                                                                                     TAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCC                                  >  SRR19973080.3723895/1‑150 (MQ=60)
                                                                                                                          CGCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAAC                             >  SRR19973080.3915360/1‑150 (MQ=60)
                                                                                                                           GCAAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACC                            >  SRR19973080.2139043/1‑150 (MQ=60)
                                                                                                                           GCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACC                            <  SRR19973080.315231/150‑1 (MQ=60)
                                                                                                                             AAAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTT                          >  SRR19973080.35819/1‑150 (MQ=60)
                                                                                                                              AAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTC                         <  SRR19973080.3389546/150‑1 (MQ=60)
                                                                                                                              AAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTC                         >  SRR19973080.1383121/1‑150 (MQ=60)
                                                                                                                              AAAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTC                         >  SRR19973080.1383098/1‑150 (MQ=60)
                                                                                                                               AAAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCG                        >  SRR19973080.4127698/1‑150 (MQ=60)
                                                                                                                                AAGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGC                       >  SRR19973080.2657230/1‑150 (MQ=60)
                                                                                                                                 AGGGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCC                      <  SRR19973080.4204324/150‑1 (MQ=60)
                                                                                                                                   GGCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGT                    >  SRR19973080.3577456/1‑150 (MQ=60)
                                                                                                                                    GCCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTT                   <  SRR19973080.2204785/150‑1 (MQ=60)
                                                                                                                                     CCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTT                  <  SRR19973080.4136671/150‑1 (MQ=60)
                                                                                                                                     CCGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTT                  <  SRR19973080.4135789/150‑1 (MQ=60)
                                                                                                                                      CGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTG                 >  SRR19973080.3334776/1‑150 (MQ=60)
                                                                                                                                      CGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTG                 >  SRR19973080.4101069/1‑150 (MQ=60)
                                                                                                                                      CGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTG                 >  SRR19973080.4280361/1‑150 (MQ=60)
                                                                                                                                      CGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTG                 >  SRR19973080.4280355/1‑150 (MQ=60)
                                                                                                                                      CGACGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTG                 >  SRR19973080.4103490/1‑150 (MQ=60)
                                                                                                                                       GACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGA                <  SRR19973080.1423729/150‑1 (MQ=60)
                                                                                                                                         CGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAAC              <  SRR19973080.1557157/150‑1 (MQ=60)
                                                                                                                                         CGGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAAC              <  SRR19973080.2623248/150‑1 (MQ=60)
                                                                                                                                          GGCAGATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACA             >  SRR19973080.1942250/1‑150 (MQ=60)
                                                                                                                                              GATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATG         <  SRR19973080.4441880/150‑1 (MQ=60)
                                                                                                                                               ATCAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGA        >  SRR19973080.1229854/1‑150 (MQ=60)
                                                                                                                                               ATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGA        <  SRR19973080.426697/150‑1 (MQ=60)
                                                                                                                                                 CAGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGT      >  SRR19973080.1463055/1‑150 (MQ=60)
                                                                                                                                                  AGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTC     >  SRR19973080.428662/1‑150 (MQ=60)
                                                                                                                                                  AGCTCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTC     >  SRR19973080.428640/1‑150 (MQ=60)
                                                                                                                                                     TCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGG  <  SRR19973080.4288386/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGG  >  CP009273/1388257‑1388555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑