Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,604,418 C→A 9.4% D174Y (GAC→TAC)  uxaB ← altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,604,4180CA 9.4% 874.5 / 50.1 244D174Y (GAC→TAC) uxaBaltronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base C (128/93);  new base A (6/17);  total (134/110)
Fisher's exact test for biased strand distribution p-value = 4.07e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 258 total aligned reads displayed.

TTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCG  >  CP009273/1604269‑1604567
                                                                                                                                                     |                                                                                                                                                     
   aTCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGtcaat                                                                                                                                                    <  2:1674339/150‑1 (MQ=255)
    tCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATGGAAGtcaatc                                                                                                                                                   >  2:842040/1‑150 (MQ=255)
            aTAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTcaca                                                                                                                                           >  1:4344008/1‑150 (MQ=255)
              aaCCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACg                                                                                                                                         <  2:4038970/150‑1 (MQ=255)
               aCCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACgg                                                                                                                                        <  2:244394/150‑1 (MQ=255)
                ccGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCTCCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGa                                                                                                                                       <  2:493810/150‑1 (MQ=255)
                  ggTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAat                                                                                                                                     <  2:2872058/150‑1 (MQ=255)
                  ggTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAat                                                                                                                                     >  2:2806893/1‑150 (MQ=255)
                  ggTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAat                                                                                                                                     >  2:2806826/1‑150 (MQ=255)
                   gTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTAAATCAACTCACACGGActg                                                                                                                                    >  2:4018182/1‑150 (MQ=255)
                      aCGATACGGGCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCCCCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAatgatg                                                                                                                                 <  2:4203423/150‑1 (MQ=255)
                        gATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATgatgat                                                                                                                               >  2:3726253/1‑150 (MQ=255)
                          tACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATcc                                                                                                                             <  1:4023139/150‑1 (MQ=255)
                          tACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTAAATCAACTCACACGGAATGATGATcc                                                                                                                             <  2:3738850/150‑1 (MQ=255)
                            cGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCaa                                                                                                                           >  1:2814554/1‑150 (MQ=255)
                             ggTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCacc                                                                                                                          >  2:1195961/1‑148 (MQ=255)
                              gTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTAGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAAATCACACGGAATGATGATCCAAcc                                                                                                                         >  1:2217298/1‑150 (MQ=255)
                                ccaccaGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCtt                                                                                                                       >  1:101538/1‑150 (MQ=255)
                                ccaccaGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCtt                                                                                                                       >  2:1468268/1‑150 (MQ=255)
                                 caccaGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTAAATCAACTCACACGGAATGATGATCCAACCttt                                                                                                                      <  2:4480804/150‑1 (MQ=255)
                                  accaGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTa                                                                                                                     >  2:868815/1‑150 (MQ=255)
                                    caGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATc                                                                                                                   >  2:2256402/1‑150 (MQ=255)
                                     aGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATcc                                                                                                                  <  2:3612643/150‑1 (MQ=255)
                                         tAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAgcg                                                                                                              <  2:1639644/150‑1 (MQ=255)
                                         tAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAgcg                                                                                                              <  1:3447974/150‑1 (MQ=255)
                                         tAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTGTATCCAgcg                                                                                                              >  1:3507437/1‑150 (MQ=255)
                                           gAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCa                                                                                                            >  1:3369906/1‑150 (MQ=255)
                                             aCAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCAcc                                                                                                          >  1:4054033/1‑150 (MQ=255)
                                              cAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCg                                                                                                         >  1:2124222/1‑150 (MQ=255)
                                                 aaGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTg                                                                                                      >  1:3521610/1‑150 (MQ=255)
                                                  aGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGa                                                                                                     >  1:1506676/1‑150 (MQ=255)
                                                  aGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGa                                                                                                     >  2:3347379/1‑150 (MQ=255)
                                                  aGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGa                                                                                                     <  1:1916297/150‑1 (MQ=255)
                                                     tGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAAt                                                                                                  >  1:3262823/1‑150 (MQ=255)
                                                     tGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAAt                                                                                                  >  2:902689/1‑150 (MQ=255)
                                                        tAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGc                                                                                               >  1:2293181/1‑150 (MQ=255)
                                                        tAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGc                                                                                               >  2:2985817/1‑150 (MQ=255)
                                                        tAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTAAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGc                                                                                               <  1:2584381/150‑1 (MQ=255)
                                                         aGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCt                                                                                              >  1:988948/1‑150 (MQ=255)
                                                            cTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTcaa                                                                                           >  2:4025290/1‑150 (MQ=255)
                                                            cTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTaaa                                                                                           >  1:3920933/1‑150 (MQ=255)
                                                             tGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAg                                                                                          >  2:4264228/1‑150 (MQ=255)
                                                               aTCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCg                                                                                        >  2:1920610/1‑150 (MQ=255)
                                                               aTCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCg                                                                                        >  2:2119464/1‑150 (MQ=255)
                                                                 ccAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGtt                                                                                      >  1:124189/1‑150 (MQ=255)
                                                                  cAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTc                                                                                     <  1:2510009/150‑1 (MQ=255)
                                                                  cAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTc                                                                                     <  2:1769683/150‑1 (MQ=255)
                                                                   aGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCg                                                                                    <  2:1033519/150‑1 (MQ=255)
                                                                   aGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTAAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCg                                                                                    <  1:588596/150‑1 (MQ=255)
                                                                      cATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAAc                                                                                 <  1:3654507/150‑1 (MQ=255)
                                                                      cATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAAc                                                                                 <  1:893647/150‑1 (MQ=255)
                                                                      cATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAAc                                                                                 <  2:3942711/150‑1 (MQ=255)
                                                                            aTAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGa                                                                           <  2:2041519/150‑1 (MQ=255)
                                                                              aaaTGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACg                                                                         >  2:4478916/1‑150 (MQ=255)
                                                                                 tGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTATTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGt                                                                      <  2:266878/150‑1 (MQ=255)
                                                                                 tGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGt                                                                      <  2:3463052/150‑1 (MQ=255)
                                                                                   cTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCa                                                                    >  2:406337/1‑150 (MQ=255)
                                                                                     tCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGt                                                                  <  1:3096795/150‑1 (MQ=255)
                                                                                       tGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGttt                                                                >  2:2846032/1‑150 (MQ=255)
                                                                                       tGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGttt                                                                >  1:569957/1‑150 (MQ=255)
                                                                                         gCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTg                                                              <  1:763321/150‑1 (MQ=255)
                                                                                           aGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGcc                                                            <  2:689642/150‑1 (MQ=255)
                                                                                            gTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTAAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCg                                                           <  2:4159757/150‑1 (MQ=255)
                                                                                             tGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCgg                                                          >  1:663425/1‑150 (MQ=255)
                                                                                               cccACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGAt                                                        <  2:1344643/150‑1 (MQ=255)
                                                                                                 cACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAg                                                      <  2:2130631/150‑1 (MQ=255)
                                                                                                 cACTCTTGCGCAGAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAg                                                      <  2:1590657/150‑1 (MQ=255)
                                                                                                  aCTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGc                                                     >  2:3274934/1‑150 (MQ=255)
                                                                                                     ctTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTa                                                  >  1:2608029/1‑150 (MQ=255)
                                                                                                     ctTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTa                                                  >  2:1092865/1‑150 (MQ=255)
                                                                                                       tGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTAcc                                                >  2:920041/1‑150 (MQ=255)
                                                                                                        gcgcAGAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCg                                               <  2:4283887/150‑1 (MQ=255)
                                                                                                         cgcATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGc                                              >  2:630786/1‑150 (MQ=255)
                                                                                                          gcATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCt                                             <  2:3185474/150‑1 (MQ=255)
                                                                                                           cATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTg                                            <  2:124189/150‑1 (MQ=255)
                                                                                                                  cagcaCCAGTTCACGCAACGCGTCGCCATTGTAGTAAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTc                                     >  2:1381635/1‑150 (MQ=255)
                                                                                                                   agcaCCAGTTCACGCAACGCGTCGCCATTGTAGTAAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCa                                    <  1:1785652/150‑1 (MQ=255)
                                                                                                                   agcaCCAGTTCACGCAACGCGTCGCCATTGTAGTAAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCa                                    <  1:4059888/150‑1 (MQ=255)
                                                                                                                      aCCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCg                                 >  2:2247986/1‑150 (MQ=255)
                                                                                                                          gTTCACGCAACGCGTCGCCATTGTAGTAAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAAtt                             <  2:3843343/150‑1 (MQ=255)
                                                                                                                           ttCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAAttt                            >  2:757914/1‑150 (MQ=255)
                                                                                                                            tCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTa                           <  2:727774/150‑1 (MQ=255)
                                                                                                                             cACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTAt                          <  2:3256800/150‑1 (MQ=255)
                                                                                                                                gCAACGCGTCGCCATTGTAGTAAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGc                       <  2:256972/150‑1 (MQ=255)
                                                                                                                                 cAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGcc                      >  2:3802568/1‑150 (MQ=255)
                                                                                                                                  aaCGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGccc                     >  2:421918/1‑150 (MQ=255)
                                                                                                                                  aaCGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGccc                     >  1:4280409/1‑150 (MQ=255)
                                                                                                                                  aaCGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGccc                     >  1:237934/1‑150 (MQ=255)
                                                                                                                                    cgcgTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGc                   >  2:1407059/1‑150 (MQ=255)
                                                                                                                                         cGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGt              >  1:2290192/1‑150 (MQ=255)
                                                                                                                                         cGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGt              >  2:893562/1‑150 (MQ=255)
                                                                                                                                          gCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTa             <  2:3644131/150‑1 (MQ=255)
                                                                                                                                           ccATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAg            <  1:2961817/150‑1 (MQ=255)
                                                                                                                                           ccATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAg            <  1:702002/150‑1 (MQ=255)
                                                                                                                                            cATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGc           <  2:4344008/150‑1 (MQ=255)
                                                                                                                                             aTTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCt          >  2:688866/1‑150 (MQ=255)
                                                                                                                                             aTTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCt          >  2:800520/1‑150 (MQ=255)
                                                                                                                                                     caatcaaCTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCg  >  1:4496617/1‑150 (MQ=255)
                                                                                                                                                     caatcaaCTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCg  <  2:3250829/150‑1 (MQ=255)
                                                                                                                                                     caatcaaCTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCg  <  2:2915220/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCG  >  CP009273/1604269‑1604567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A