Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,730,615 C→A 7.3% intergenic (‑78/‑242) bax ← / → malS putative glucosaminidase/alpha‑amylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,730,6150CA 7.3% 929.7 / 32.3 247intergenic (‑78/‑242)bax/malSputative glucosaminidase/alpha‑amylase
Reads supporting (aligned to +/- strand):  ref base C (110/118);  new base A (8/11);  total (118/129)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

CTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATCGCTCACCCTTGCTT  >  CP009273/3730466‑3730764
                                                                                                                                                     |                                                                                                                                                     
cTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATc                                                                                                                                                       <  2:1564046/150‑1 (MQ=255)
   aCTCGAAAACACCAGAGTGAGTAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCAAAAATTTGAGAGTTGAATATCAAATCATATaaaa                                                                                                                                                    >  1:2412887/1‑150 (MQ=255)
       gAAAACACCAGAGTGAGTAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATa                                                                                                                                                >  1:458413/1‑150 (MQ=255)
       gAAAACACCAGAGTGAGTAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATa                                                                                                                                                >  2:1163333/1‑150 (MQ=255)
        aaaaCACCAGAGTGAGTAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAg                                                                                                                                               <  2:948054/150‑1 (MQ=255)
         aaaCACCAGAGTGAGTAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGc                                                                                                                                              <  1:4098046/150‑1 (MQ=255)
            cacCAGAGTGAGTAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGt                                                                                                                                           <  2:270345/150‑1 (MQ=255)
             acCAGAGTGAGTAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTc                                                                                                                                          >  2:3232551/1‑150 (MQ=255)
              ccAGAGTGAGTAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATAAAAAATAGCTGTCa                                                                                                                                         >  1:1301081/1‑150 (MQ=255)
                 gagtgagtAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAaga                                                                                                                                      >  1:1613591/1‑150 (MQ=255)
                  agtgagtAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAagag                                                                                                                                     <  2:4205567/150‑1 (MQ=255)
                   gtgagtAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATATCTGTCAAGAGc                                                                                                                                    >  1:3038340/1‑150 (MQ=255)
                      agtAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGTATCTCAAATCATATCAAAAATAGCTGTCAAGAGCAcc                                                                                                                                 >  1:4232724/1‑150 (MQ=255)
                        tAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCAcccc                                                                                                                               >  1:1026113/1‑150 (MQ=255)
                         aaCATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCa                                                                                                                              >  2:3871486/1‑150 (MQ=255)
                           cATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAg                                                                                                                            >  2:2938449/1‑150 (MQ=255)
                                  atcatcGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGt                                                                                                                     <  2:507986/150‑1 (MQ=255)
                                  atcatcGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGt                                                                                                                     >  2:4178441/1‑150 (MQ=255)
                                   tcatcGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTc                                                                                                                    <  1:43117/150‑1 (MQ=255)
                                       cGCCCCATATCGTCGTATGGGTGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCaaa                                                                                                                <  2:3386015/150‑1 (MQ=255)
                                             atatCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGaa                                                                                                          >  2:962321/1‑150 (MQ=255)
                                              tatCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGaaa                                                                                                         <  1:633146/150‑1 (MQ=255)
                                                tcgtcgTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACt                                                                                                       <  2:3554914/150‑1 (MQ=255)
                                                 cgtcgtATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTa                                                                                                      <  2:1812486/150‑1 (MQ=255)
                                                 cgtcgtATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTa                                                                                                      >  1:3736660/1‑150 (MQ=255)
                                                 cgtcgtATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTa                                                                                                      <  2:3093209/150‑1 (MQ=255)
                                                  gtcgtATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTAt                                                                                                     <  2:2783407/150‑1 (MQ=255)
                                                    cgtATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGt                                                                                                   >  2:140797/1‑150 (MQ=255)
                                                         gggAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACgt                                                                                              >  1:4444146/1‑150 (MQ=255)
                                                          ggAGTCAAACTCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACgtg                                                                                             <  2:2852479/150‑1 (MQ=255)
                                                          ggAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACgtg                                                                                             >  1:598935/1‑150 (MQ=255)
                                                            aGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGtt                                                                                           <  2:4309120/150‑1 (MQ=255)
                                                             gTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATAAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTa                                                                                          <  1:2579291/150‑1 (MQ=255)
                                                               cAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAAc                                                                                        <  2:2693159/150‑1 (MQ=255)
                                                               aaaaaTCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAAc                                                                                        <  2:4272349/149‑1 (MQ=255)
                                                                 aaaTCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGa                                                                                      >  1:1040128/1‑150 (MQ=255)
                                                                   atcatcAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATATCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTCTGTCACGTGTTAACGAtt                                                                                    >  2:4223696/1‑150 (MQ=255)
                                                                   atcatcAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGAtt                                                                                    <  2:1041025/150‑1 (MQ=255)
                                                                     catcaGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCa                                                                                  >  2:1378655/1‑150 (MQ=255)
                                                                          ggTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTg                                                                             >  2:3522381/1‑150 (MQ=255)
                                                                          ggTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTg                                                                             <  1:4535283/150‑1 (MQ=255)
                                                                          ggTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTg                                                                             <  1:734997/150‑1 (MQ=255)
                                                                            tctcCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGgc                                                                           >  2:3682730/1‑150 (MQ=255)
                                                                            tctcCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGgc                                                                           <  2:458431/150‑1 (MQ=255)
                                                                             ctcCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGgcg                                                                          >  2:2625549/1‑150 (MQ=255)
                                                                                cTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTa                                                                       <  1:209325/150‑1 (MQ=255)
                                                                                cTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATAAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTa                                                                       <  2:1024847/150‑1 (MQ=255)
                                                                                    tCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATc                                                                   <  1:1148272/150‑1 (MQ=255)
                                                                                    tCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATc                                                                   <  1:3958829/150‑1 (MQ=255)
                                                                                     cGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCg                                                                  >  2:850105/1‑150 (MQ=255)
                                                                                     cGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCg                                                                  >  2:3009358/1‑150 (MQ=255)
                                                                                       gATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCa                                                                <  1:333019/150‑1 (MQ=255)
                                                                                        aTTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGGGCTAAATCGctg                                                               >  2:4418629/1‑148 (MQ=255)
                                                                                         tttAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGa                                                              <  1:3077222/150‑1 (MQ=255)
                                                                                          tttaTCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGaa                                                             <  1:2219314/147‑1 (MQ=255)
                                                                                          ttAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGaa                                                             <  2:2305142/150‑1 (MQ=255)
                                                                                            aaTCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGATTCTCAAATCATATCAAAAATAGCTGTCCAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGaaaa                                                           >  2:4369888/1‑150 (MQ=255)
                                                                                             aTCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAAt                                                          <  2:2130288/150‑1 (MQ=255)
                                                                                              tCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAAtg                                                         <  1:2818067/150‑1 (MQ=255)
                                                                                               cATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAAtgt                                                        >  1:2143990/1‑150 (MQ=255)
                                                                                               cATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAAtgt                                                        <  1:978605/150‑1 (MQ=255)
                                                                                                 ttCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTAGGTCACGTGTTAACGATTCAGAGTGGCGCTAAATCGCAGAAAATGTgg                                                      >  2:3349080/1‑150 (MQ=255)
                                                                                                    cAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTggggg                                                   <  2:2412778/150‑1 (MQ=255)
                                                                                                      acacCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGtt                                                 >  1:2622882/1‑150 (MQ=255)
                                                                                                       cacCTTATATTTTTCACAAATTTGAGAGTTGACTCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACGATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTa                                                >  1:1788150/1‑150 (MQ=255)
                                                                                                        acCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTAt                                               <  1:1520174/150‑1 (MQ=255)
                                                                                                        acCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTAt                                               <  2:4082977/150‑1 (MQ=255)
                                                                                                        acCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTAt                                               >  2:1875393/1‑150 (MQ=255)
                                                                                                         ccTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATc                                              <  1:233350/150‑1 (MQ=255)
                                                                                                         ccTTATATTGTTCACAAATTTGAGAGTGGACTCTCAATGCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGGGGTAACGATGCCGATTGGCGCTAAATCGCAGAAAAGGGGGGGGGTATc                                              >  2:2196355/1‑150 (MQ=255)
                                                                                                              tatTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCaaa                                         >  1:3152212/1‑150 (MQ=255)
                                                                                                              tatTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCaaa                                         >  1:910417/1‑150 (MQ=255)
                                                                                                               atTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCaaaa                                        >  1:2815674/1‑150 (MQ=255)
                                                                                                               atTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCaaaa                                        <  1:1795943/150‑1 (MQ=255)
                                                                                                                     cacaAATTTGAGAGTTGAATCTCAAATCATCTCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGc                                  <  1:3365359/150‑1 (MQ=255)
                                                                                                                     cacaAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGc                                  >  2:3674937/1‑150 (MQ=255)
                                                                                                                     cacaAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGc                                  <  1:3432116/150‑1 (MQ=255)
                                                                                                                     cacaAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGc                                  <  1:1083332/150‑1 (MQ=255)
                                                                                                                       caAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCg                                <  1:1958706/150‑1 (MQ=255)
                                                                                                                       caAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCg                                <  1:3847120/150‑1 (MQ=255)
                                                                                                                        aaaTTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGt                               >  2:1125568/1‑150 (MQ=255)
                                                                                                                          aTTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGttt                             <  1:765026/150‑1 (MQ=255)
                                                                                                                            ttGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGttttt                           <  2:681757/150‑1 (MQ=255)
                                                                                                                              gagaGTTGAATCTCAAATCATATAAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTg                         <  2:1301081/150‑1 (MQ=255)
                                                                                                                               agagTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTgc                        <  2:3128267/150‑1 (MQ=255)
                                                                                                                                gagTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTgcg                       <  2:2051537/150‑1 (MQ=255)
                                                                                                                                  gTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGcgcg                     <  2:2246079/150‑1 (MQ=255)
                                                                                                                                      aaTCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGAt                 >  2:2675423/1‑150 (MQ=255)
                                                                                                                                       aTCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATc                <  2:3924483/150‑1 (MQ=255)
                                                                                                                                       aTCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATc                >  1:3781094/1‑150 (MQ=255)
                                                                                                                                       aTCTCAAATCATATAAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATc                <  1:4216174/150‑1 (MQ=255)
                                                                                                                                        tctcAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATCg               >  2:3036858/1‑150 (MQ=255)
                                                                                                                                        tctcAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATCg               <  2:2000671/150‑1 (MQ=255)
                                                                                                                                         ctcAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATCGc              >  2:2084396/1‑150 (MQ=255)
                                                                                                                                          tcAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATCGCt             <  1:3682763/150‑1 (MQ=255)
                                                                                                                                          tcAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATCGCt             >  1:3588221/1‑150 (MQ=255)
                                                                                                                                          tcAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATCGCt             <  1:1809250/150‑1 (MQ=255)
                                                                                                                                            aaaTCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATCGCTCa           <  1:1408917/150‑1 (MQ=255)
                                                                                                                                            aaaTCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATCGCTCa           <  1:2225977/150‑1 (MQ=255)
                                                                                                                                              aTCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATCGCTCAcc         >  1:3198375/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATCGCTCACCCTTGCTT  >  CP009273/3730466‑3730764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A