Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 648,317 G→T 20.1% M209I (ATG→ATT)  citA → sensory histidine kinase in two‑component regulatory system with CitB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273648,3170GT20.1% 518.5 / 83.4 193M209I (ATG→ATT) citAsensory histidine kinase in two‑component regulatory system with CitB
Reads supporting (aligned to +/- strand):  ref base G (79/75);  new base T (18/21);  total (97/96)
Fisher's exact test for biased strand distribution p-value = 5.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

CGGCGTGGTGTCGATTGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGCTGGGGCTGAGT  >  CP009273/648170‑648464
                                                                                                                                                   |                                                                                                                                                   
 ggCGTGGTGTCGATTGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAgatga                                                                                                                                                    <  2:2057358/148‑1 (MQ=255)
  gCGTGGTGTCGATTGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAgatgat                                                                                                                                                   <  2:1665009/148‑1 (MQ=255)
  gCGTGGTGTCGATTGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAgatgat                                                                                                                                                   <  2:2851046/148‑1 (MQ=255)
      ggtgTCGATTGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGc                                                                                                                                               <  1:1146698/148‑1 (MQ=255)
         gtCGATTGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATg                                                                                                                                            <  1:3039656/148‑1 (MQ=255)
         gtCGATTGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATg                                                                                                                                            >  2:627011/1‑148 (MQ=255)
         gtCGATTGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATg                                                                                                                                            >  1:2117506/1‑148 (MQ=255)
            gATTGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAg                                                                                                                                         <  1:845009/148‑1 (MQ=255)
             aTTGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGc                                                                                                                                        >  2:2100563/1‑148 (MQ=255)
             aTTGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGc                                                                                                                                        <  1:2567384/148‑1 (MQ=255)
             aTTGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCGGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCAAATACGTCGGCAGATGATGTGCATGGAGc                                                                                                                                        >  1:897992/1‑148 (MQ=255)
              ttGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGcc                                                                                                                                       >  1:2237297/1‑148 (MQ=255)
                 gCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCaaa                                                                                                                                    >  1:1453704/1‑148 (MQ=255)
                     ccTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCaa                                                                                                                                <  1:1278155/148‑1 (MQ=255)
                     ccTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCaa                                                                                                                                <  2:858557/148‑1 (MQ=255)
                     ccTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCaa                                                                                                                                <  2:500547/148‑1 (MQ=255)
                     ccTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCaa                                                                                                                                <  2:2608882/148‑1 (MQ=255)
                     ccTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCaa                                                                                                                                >  1:1994317/1‑148 (MQ=255)
                       tGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAAt                                                                                                                              <  2:2187821/148‑1 (MQ=255)
                                  aTCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTgg                                                                                                                   <  1:3298912/148‑1 (MQ=255)
                                  aTCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTgg                                                                                                                   >  2:2414715/1‑148 (MQ=255)
                                   tCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGt                                                                                                                  >  1:1105833/1‑148 (MQ=255)
                                      aTAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCg                                                                                                               >  2:1846934/1‑148 (MQ=255)
                                          cTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAg                                                                                                           >  2:2243552/1‑148 (MQ=255)
                                          cTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAg                                                                                                           >  1:2807846/1‑148 (MQ=255)
                                          cTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAg                                                                                                           >  2:2243572/1‑148 (MQ=255)
                                            ggCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTAGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCa                                                                                                         <  2:1405990/148‑1 (MQ=255)
                                            ggCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTAGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCa                                                                                                         <  2:2379159/148‑1 (MQ=255)
                                              cGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAag                                                                                                       <  1:237064/148‑1 (MQ=255)
                                                ggCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAagag                                                                                                     >  1:469448/1‑148 (MQ=255)
                                                 gCTGAGTTTTTATTACCGATGGCCGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAgg                                                                                                    >  2:927607/1‑148 (MQ=255)
                                                   tGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCACGAGgcg                                                                                                  >  2:1683729/1‑148 (MQ=255)
                                                    gAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGgcgc                                                                                                 >  2:165857/1‑148 (MQ=255)
                                                    gAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGgcgc                                                                                                 >  2:3283145/1‑148 (MQ=255)
                                                    gAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGgcgc                                                                                                 <  2:932329/148‑1 (MQ=255)
                                                     aGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCt                                                                                                <  2:274354/148‑1 (MQ=255)
                                                      gTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTg                                                                                               <  1:259851/148‑1 (MQ=255)
                                                       tttttATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGt                                                                                              >  2:2512177/1‑148 (MQ=255)
                                                       tttttATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGt                                                                                              <  2:1954999/148‑1 (MQ=255)
                                                       tttttATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGt                                                                                              <  1:837516/148‑1 (MQ=255)
                                                           tattaCCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAg                                                                                          <  2:1879380/148‑1 (MQ=255)
                                                            attaCCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGt                                                                                         <  1:1936501/148‑1 (MQ=255)
                                                             ttaCCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGtt                                                                                        >  1:1408300/1‑148 (MQ=255)
                                                                ccGATGGAAGGTGTGTTTGTCGTGCTGTTTGTGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACTCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCgg                                                                                     <  2:658346/148‑1 (MQ=255)
                                                                 cGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGt                                                                                    <  1:2996876/148‑1 (MQ=255)
                                                                 cGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGt                                                                                    <  1:1349312/148‑1 (MQ=255)
                                                                 cGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGt                                                                                    <  1:162103/148‑1 (MQ=255)
                                                                 cGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGt                                                                                    <  2:159150/148‑1 (MQ=255)
                                                                   aTGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGttt                                                                                  >  1:521191/1‑148 (MQ=255)
                                                                   aTGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGttt                                                                                  >  1:502941/1‑148 (MQ=255)
                                                                    tGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTa                                                                                 <  2:2236124/148‑1 (MQ=255)
                                                                    tGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTa                                                                                 <  2:1532258/148‑1 (MQ=255)
                                                                          gtgtgtTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAgg                                                                           >  2:1637807/1‑148 (MQ=255)
                                                                           tgtgtTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAggg                                                                          >  1:2119469/1‑148 (MQ=255)
                                                                            gtgtTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGc                                                                         <  2:2285511/148‑1 (MQ=255)
                                                                              gtTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTg                                                                       >  2:1315124/1‑148 (MQ=255)
                                                                               tttGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGa                                                                      <  2:1178349/148‑1 (MQ=255)
                                                                               tttGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGa                                                                      >  1:1897639/1‑148 (MQ=255)
                                                                                  gTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTg                                                                   <  1:170118/148‑1 (MQ=255)
                                                                                  gTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTg                                                                   <  1:743664/148‑1 (MQ=255)
                                                                                     gTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCgg                                                                <  1:430071/148‑1 (MQ=255)
                                                                                          gTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGAt                                                           <  1:698050/148‑1 (MQ=255)
                                                                                            tAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATcc                                                         <  2:2839821/148‑1 (MQ=255)
                                                                                            tAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATcc                                                         >  2:1210382/1‑148 (MQ=255)
                                                                                              gggATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGc                                                       <  1:610740/148‑1 (MQ=255)
                                                                                               ggATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCa                                                      <  2:1944243/148‑1 (MQ=255)
                                                                                                  ttCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATgg                                                   <  1:374845/148‑1 (MQ=255)
                                                                                                   tCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGt                                                  >  2:3149861/1‑148 (MQ=255)
                                                                                                    cTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGtt                                                 >  1:3230208/1‑148 (MQ=255)
                                                                                                        tGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACAt                                             >  2:597828/1‑148 (MQ=255)
                                                                                                         gTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACAtt                                            <  2:122666/148‑1 (MQ=255)
                                                                                                                tCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCa                                     >  1:1222238/1‑148 (MQ=255)
                                                                                                                tCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCa                                     >  1:1411700/1‑148 (MQ=255)
                                                                                                                 cGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCAt                                    <  2:1766567/148‑1 (MQ=255)
                                                                                                                 cGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCAt                                    <  2:2563822/148‑1 (MQ=255)
                                                                                                                 cGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGccct                                    >  1:304596/1‑146 (MQ=255)
                                                                                                                 cGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCAt                                    <  2:2237297/148‑1 (MQ=255)
                                                                                                                   tGGTTCCTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCCTGGTTACATTACCGCCATCa                                  >  1:712707/1‑148 (MQ=255)
                                                                                                                    ggTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCaa                                 >  2:1870637/1‑148 (MQ=255)
                                                                                                                    ggTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCaa                                 <  1:1835197/148‑1 (MQ=255)
                                                                                                                    ggTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCaa                                 <  1:2162124/148‑1 (MQ=255)
                                                                                                                    ggTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCaa                                 >  2:2068719/1‑148 (MQ=255)
                                                                                                                       tCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCGGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCg                              >  2:1095189/1‑148 (MQ=255)
                                                                                                                        ccTGGCCGCGCATATCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGt                             <  1:1054258/148‑1 (MQ=255)
                                                                                                                         cTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTa                            <  1:432236/148‑1 (MQ=255)
                                                                                                                                    tatCCGTCGGCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCaagaaag                 <  1:623162/148‑1 (MQ=255)
                                                                                                                                    tatCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCaagaaag                 >  2:1007989/1‑148 (MQ=255)
                                                                                                                                     atCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCaagaaaga                >  2:1469413/1‑148 (MQ=255)
                                                                                                                                        cGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGc             <  2:2781068/148‑1 (MQ=255)
                                                                                                                                        cGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGc             <  1:2348381/148‑1 (MQ=255)
                                                                                                                                         gTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGCt            <  1:1092435/148‑1 (MQ=255)
                                                                                                                                          tCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGCTg           >  2:736099/1‑148 (MQ=255)
                                                                                                                                           cGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGCTgg          >  1:1806366/1‑148 (MQ=255)
                                                                                                                                             gCAGATTATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGCTgggg        >  2:318364/1‑148 (MQ=255)
                                                                                                                                               aGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGCTGGGGCt      <  2:3108819/148‑1 (MQ=255)
                                                                                                                                                gattatGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGCTGGGGCTg     <  1:821429/148‑1 (MQ=255)
                                                                                                                                                gatgatGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGCTGGGGCTg     >  1:318305/1‑148 (MQ=255)
                                                                                                                                                 atgatgGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGCTGGGGCTGa    >  1:1672623/1‑148 (MQ=255)
                                                                                                                                                   gatgGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGCTGGGGCTGAGt  >  1:2445833/1‑148 (MQ=255)
                                                                                                                                                   gatgGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGCTGGGGCTGAGt  >  1:1233845/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGGCGTGGTGTCGATTGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTATTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTCCTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTGGTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCGCATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGCTGGGGCTGAGT  >  CP009273/648170‑648464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A