Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 817,100 A→T 26.0% R285R (CGT→CGA)  ybhN ← UPF0104 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273817,1000AT26.0% 341.8 / 137.1 188R285R (CGT→CGA) ybhNUPF0104 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (71/68);  new base T (22/27);  total (93/95)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 189 total aligned reads displayed.

TTTTCATTAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTAACCAGTAACACGCC  >  CP009273/816953‑817243
                                                                                                                                                   |                                                                                                                                               
ttttCATTAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCTCCCGTAGCTTATTCGCCTGGCTATCCAGCAAAACGTAAAAGATAAGCGCCAGCAGCAGAGTGAAAAAGTAAAACAGcaca                                                                                                                                                 >  1:1515702/1‑148 (MQ=255)
    cATTAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGt                                                                                                                                             <  1:2760208/148‑1 (MQ=255)
      ttAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTGATTTTTCGCCCGTAGCTTCTTAGCCTGGCTTTGCAGCAACACGTAACACATCAGCCCCAGCACCCCTGGGGTAAATTAATACAGACCACCGTaa                                                                                                                                           >  1:17847/1‑148 (MQ=255)
       tAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAg                                                                                                                                          <  2:2967029/148‑1 (MQ=255)
       tAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAg                                                                                                                                          <  2:2966694/148‑1 (MQ=255)
        aGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGc                                                                                                                                         >  1:2203625/1‑148 (MQ=255)
           cGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAg                                                                                                                                      <  2:2821467/148‑1 (MQ=255)
            gTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGt                                                                                                                                     >  1:1843032/1‑148 (MQ=255)
             tAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTa                                                                                                                                    <  1:2630129/148‑1 (MQ=255)
               aaTGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAgg                                                                                                                                  >  1:2464384/1‑148 (MQ=255)
                 tGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGggc                                                                                                                                >  2:682113/1‑148 (MQ=255)
                     caTACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGgcggcg                                                                                                                            >  1:3000368/1‑148 (MQ=255)
                     caTACTAAAGAACTTAACTAAACTTAACATCGCCGCTTCAATTTTAGCCCGTAGCTTAATCCACTGGCATTACACCAACATCTAACACAAAAGCCCCACAAGCAGAGTGATAAAGTAAAACAGCACACGGTAAACGAAAAGGgcggcg                                                                                                                            >  2:1496328/1‑148 (MQ=255)
                      aTACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGa                                                                                                                           <  1:2298465/148‑1 (MQ=255)
                       tACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGAt                                                                                                                          <  2:2857735/148‑1 (MQ=255)
                       tACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTAGCCTGGCTTTCCAGCAACAGGTAACACATCAGCGCCAGCACCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGAt                                                                                                                          >  1:2644655/1‑148 (MQ=255)
                         cTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATaa                                                                                                                        >  2:2073344/1‑148 (MQ=255)
                          tAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAAt                                                                                                                       >  1:3246319/1‑148 (MQ=255)
                          tAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAAt                                                                                                                       >  1:1383709/1‑148 (MQ=255)
                            aaGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTg                                                                                                                     <  2:274582/148‑1 (MQ=255)
                            aaGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTg                                                                                                                     <  1:1484904/148‑1 (MQ=255)
                            aaGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTg                                                                                                                     >  2:3246960/1‑148 (MQ=255)
                              gAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTa                                                                                                                   <  2:1267443/148‑1 (MQ=255)
                               aaCTTAACTAAACTTCACATCGCCGCTTCATTTTTGGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTaa                                                                                                                  >  1:1037341/1‑147 (MQ=255)
                                aCTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTAcc                                                                                                                 <  2:403329/148‑1 (MQ=255)
                                aCTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTAcc                                                                                                                 >  2:226025/1‑148 (MQ=255)
                                  ttAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACCGGTAACAGATCAGCGCCAGCCGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCtt                                                                                                               >  1:508094/1‑148 (MQ=255)
                                     aCTAAACTTCACATCGCCGCTTCATTTTTAGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCGTTgg                                                                                                            >  1:2074937/1‑148 (MQ=255)
                                        aaaCTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAAAAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAgg                                                                                                         >  2:2727091/1‑148 (MQ=255)
                                          aCTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTa                                                                                                       <  2:2537029/148‑1 (MQ=255)
                                          aCTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTa                                                                                                       <  2:2536945/148‑1 (MQ=255)
                                          aCTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTa                                                                                                       <  2:1211804/148‑1 (MQ=255)
                                           cTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTAt                                                                                                      >  2:2212666/1‑148 (MQ=255)
                                            ttCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATg                                                                                                     <  2:2827817/148‑1 (MQ=255)
                                                 aTCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTccc                                                                                                <  2:2458252/148‑1 (MQ=255)
                                                 aTCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTccc                                                                                                <  1:649296/148‑1 (MQ=255)
                                                   cgccgcTTCAATTTTAGCCCGTATCTTAATAGCCTTGCTTTCCAGCAAAAGGTAAAAGAACAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAAATTTAACTTTGGATGTATTCTACcca                                                                                              >  2:2157505/1‑148 (MQ=255)
                                                   cgccgcGTCATTTTTCGCCCGTAGCTTCGTAGCCTGGCGTTACAGCAACAGGTAACAGAACAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCCAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTccccc                                                                                              >  2:2430431/1‑147 (MQ=255)
                                                       gcTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCccagcca                                                                                          >  2:130340/1‑148 (MQ=255)
                                                         ttCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCagt                                                                                        >  1:907705/1‑148 (MQ=255)
                                                          tCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCagta                                                                                       >  1:2016123/1‑148 (MQ=255)
                                                          tCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCagta                                                                                       >  2:1790199/1‑148 (MQ=255)
                                                          tCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCagta                                                                                       >  2:35640/1‑148 (MQ=255)
                                                           cATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCagtag                                                                                      <  2:508771/148‑1 (MQ=255)
                                                             tttttCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTg                                                                                    <  1:3143810/148‑1 (MQ=255)
                                                             tttttCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTg                                                                                    <  1:467779/148‑1 (MQ=255)
                                                                   gcgcGTAGCTTCTTCGGCTGGCTTTCCATCAACAGGTAACAGATCAGCGCCAGCAGCAGTGTGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATaa                                                                              <  2:2337218/145‑1 (MQ=255)
                                                                          gCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGc                                                                       >  1:502305/1‑148 (MQ=255)
                                                                             tcttcGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTc                                                                    <  1:2794274/148‑1 (MQ=255)
                                                                              cttcGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTcc                                                                   >  1:1246709/1‑148 (MQ=255)
                                                                                tcGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAg                                                                 <  2:1033662/148‑1 (MQ=255)
                                                                                 cGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGc                                                                <  2:428090/148‑1 (MQ=255)
                                                                                    cTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGcaca                                                             <  2:1183155/148‑1 (MQ=255)
                                                                                     tGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCacac                                                            <  1:2949514/148‑1 (MQ=255)
                                                                                     tGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCacac                                                            <  1:3179230/148‑1 (MQ=255)
                                                                                       gCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCg                                                          <  2:1843032/148‑1 (MQ=255)
                                                                                        cTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGa                                                         <  2:731958/148‑1 (MQ=255)
                                                                                            ccAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATccc                                                     <  2:2046189/148‑1 (MQ=255)
                                                                                             cAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATcccc                                                    >  2:446782/1‑148 (MQ=255)
                                                                                              aGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCccg                                                   >  2:365550/1‑148 (MQ=255)
                                                                                                  aCAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCgg                                               >  2:2015758/1‑148 (MQ=255)
                                                                                                  aCAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCgg                                               >  2:2017784/1‑148 (MQ=255)
                                                                                                   cAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGa                                              <  2:2936961/148‑1 (MQ=255)
                                                                                                   cAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGa                                              <  1:3049184/148‑1 (MQ=255)
                                                                                                   cAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGa                                              <  1:2315494/148‑1 (MQ=255)
                                                                                                   cAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGa                                              <  1:438052/148‑1 (MQ=255)
                                                                                                      gTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAata                                           <  2:3148496/148‑1 (MQ=255)
                                                                                                        aaCAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATg                                         >  1:384972/1‑148 (MQ=255)
                                                                                                         aCAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGc                                        <  2:3106055/148‑1 (MQ=255)
                                                                                                           aGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCAc                                      >  1:3278990/1‑148 (MQ=255)
                                                                                                           aGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCAc                                      >  1:740672/1‑148 (MQ=255)
                                                                                                             aTCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGa                                    >  2:555974/1‑148 (MQ=255)
                                                                                                             aTCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGa                                    <  1:1750155/148‑1 (MQ=255)
                                                                                                               cAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATg                                  <  1:1013725/148‑1 (MQ=255)
                                                                                                               cAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATg                                  <  2:17847/148‑1 (MQ=255)
                                                                                                               cAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATg                                  <  1:980572/148‑1 (MQ=255)
                                                                                                                aGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGa                                 <  1:764798/148‑1 (MQ=255)
                                                                                                                  cgcCAGCAGCAGTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACg                               >  1:2336126/1‑148 (MQ=255)
                                                                                                                   gcCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGc                              <  1:1790230/148‑1 (MQ=255)
                                                                                                                     cagcagcagTGGGATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCa                            <  1:2299923/148‑1 (MQ=255)
                                                                                                                     cagcagcagTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCa                            >  2:901177/1‑148 (MQ=255)
                                                                                                                     cagcagcagTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCa                            >  1:2030870/1‑148 (MQ=255)
                                                                                                                           cagTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAAta                      >  2:653515/1‑148 (MQ=255)
                                                                                                                              tGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAAtacta                   >  2:3036114/1‑148 (MQ=255)
                                                                                                                              tGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAAtacta                   >  2:1062026/1‑148 (MQ=255)
                                                                                                                                 gATAAAGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATActacta                >  2:2294595/1‑148 (MQ=255)
                                                                                                                                  aTAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTaa               >  1:2530115/1‑148 (MQ=255)
                                                                                                                                  aTAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTaa               >  2:805293/1‑148 (MQ=255)
                                                                                                                                   tAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTAAc              >  2:1667158/1‑148 (MQ=255)
                                                                                                                                    aaaGTAATACAGCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTAAcc             <  2:1791200/148‑1 (MQ=255)
                                                                                                                                      aGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTAACCAg           >  1:2155943/1‑148 (MQ=255)
                                                                                                                                      aGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTAACCAg           >  1:618909/1‑148 (MQ=255)
                                                                                                                                      aGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTAACCAg           <  2:2644655/148‑1 (MQ=255)
                                                                                                                                        tAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTAACCAGTa         <  2:784037/148‑1 (MQ=255)
                                                                                                                                         aaTACAGCTCTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTAACCAGTaa        <  2:1593072/148‑1 (MQ=255)
                                                                                                                                          aTACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTAACCAGTAac       <  1:417745/148‑1 (MQ=255)
                                                                                                                                           tACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTAACCAGTAaca      <  2:1383709/148‑1 (MQ=255)
                                                                                                                                            aCAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTAACCAGTAacac     >  1:1805080/1‑148 (MQ=255)
                                                                                                                                            aCAGCACACGGTAAGCGAGTAGGGCGGCGATAAATGTACCATTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTACAGAACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTAACCAGTAacac     >  1:1805068/1‑148 (MQ=255)
                                                                                                                                               gCACTCGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTAACCAGTAACACGcc  >  2:1461527/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TTTTCATTAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAGTAATACAGCACACGGTAAGCGAGTAGGGCGGCGATAATTGTACCTTTGGAGGTATGCTCCCCAGCCAGTAGTGCGATAAACACCGCTTCCAGCACACCGATCCCCGCCGGAATATGCACGATGACGCCAGCAATACTACTAACCAGTAACACGCC  >  CP009273/816953‑817243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A