Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,334,048 G→T 16.2% E154D (GAG→GAT)  pgpB → phosphatidylglycerophosphatase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,334,0480GT16.2% 522.5 / 57.7 173E154D (GAG→GAT) pgpBphosphatidylglycerophosphatase B
Reads supporting (aligned to +/- strand):  ref base G (90/55);  new base T (14/14);  total (104/69)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 193 total aligned reads displayed.

TTTTGTTATCTGGCTGGAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCCTGCT  >  CP009273/1333901‑1334194
                                                                                                                                                   |                                                                                                                                                  
ttttGTTATCTGGCTGGAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAagag                                                                                                                                                    <  2:830704/148‑1 (MQ=255)
ttttGTTATCTGGCTGGAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAagag                                                                                                                                                    >  2:2907795/1‑148 (MQ=255)
 tttGTTATCTGGCTGGAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAgaga                                                                                                                                                   <  2:1264656/148‑1 (MQ=255)
     ttATCTGGCTGGAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACggg                                                                                                                                               >  2:2880249/1‑148 (MQ=255)
      tATCTGGCTGGAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACgggg                                                                                                                                              <  2:3012186/148‑1 (MQ=255)
      tATCTGGCTGGAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACgggg                                                                                                                                              >  1:1961643/1‑148 (MQ=255)
      tATCTGGCTGGAAAAAACACATCATAGTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGATACgggg                                                                                                                                              <  2:1797880/148‑1 (MQ=255)
       aTCTGGCTGGAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGATACGGGGt                                                                                                                                             <  2:1001663/148‑1 (MQ=255)
        tCTGGCTGGAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGtt                                                                                                                                            >  1:1823327/1‑148 (MQ=255)
         ctggctggAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGttt                                                                                                                                           >  2:964540/1‑148 (MQ=255)
         ctggctggAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGttt                                                                                                                                           >  1:940543/1‑148 (MQ=255)
               ggAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGATACGGGGTTTGCCttt                                                                                                                                     <  1:614046/148‑1 (MQ=255)
               ggAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCttt                                                                                                                                     <  2:1658496/148‑1 (MQ=255)
                gAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTttc                                                                                                                                    <  2:1367646/148‑1 (MQ=255)
                 aaaaaaCACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTttcc                                                                                                                                   >  1:2078753/1‑148 (MQ=255)
                    aaaCACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTttccttc                                                                                                                                <  1:103500/148‑1 (MQ=255)
                      acacATCATATTCCGGTTGATGAGTTCTACACTTTAACGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCg                                                                                                                              <  1:1516106/148‑1 (MQ=255)
                      acacATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCg                                                                                                                              >  2:1203602/1‑148 (MQ=255)
                      acacATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCg                                                                                                                              >  1:381642/1‑148 (MQ=255)
                       cacaTCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGATACGGGGTTTGCCTTTCCTTCCgg                                                                                                                             >  1:1703133/1‑148 (MQ=255)
                         catcatATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTc                                                                                                                           >  2:2305969/1‑148 (MQ=255)
                         catcatATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTc                                                                                                                           >  1:2621532/1‑148 (MQ=255)
                            catATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTcaca                                                                                                                        >  2:90328/1‑148 (MQ=255)
                            catATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTcaca                                                                                                                        >  2:2554240/1‑148 (MQ=255)
                              tatTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGATACGGGGTTTGCCTTTCCTTCCGGTCACACg                                                                                                                      <  1:2759727/148‑1 (MQ=255)
                              tatTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACg                                                                                                                      >  2:670670/1‑148 (MQ=255)
                               atTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGa                                                                                                                     >  2:2309096/1‑148 (MQ=255)
                               atTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGa                                                                                                                     >  2:180632/1‑148 (MQ=255)
                               atTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGa                                                                                                                     <  1:52966/148‑1 (MQ=255)
                               atTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGa                                                                                                                     <  1:1730318/148‑1 (MQ=255)
                                 tCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATg                                                                                                                   >  1:1153519/1‑148 (MQ=255)
                                 tCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATg                                                                                                                   >  1:2804940/1‑148 (MQ=255)
                                    ggTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGttt                                                                                                                >  1:427631/1‑148 (MQ=255)
                                       tgatgaGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTtgct                                                                                                             >  1:2058236/1‑148 (MQ=255)
                                             gTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGt                                                                                                       <  1:3242741/148‑1 (MQ=255)
                                                      tttAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCActg                                                                                              >  2:2184205/1‑148 (MQ=255)
                                                         aaaGCGAGCAGAACGCGTAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCActgctg                                                                                           <  1:1698093/148‑1 (MQ=255)
                                                          aaGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGATACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTgg                                                                                          >  2:738048/1‑148 (MQ=255)
                                                             cGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCg                                                                                       <  1:1059776/148‑1 (MQ=255)
                                                             cGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCg                                                                                       <  2:531273/148‑1 (MQ=255)
                                                                gCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTg                                                                                    >  2:2129398/1‑148 (MQ=255)
                                                                 cAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTgg                                                                                   >  1:3284890/1‑148 (MQ=255)
                                                                   gAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGtt                                                                                 >  2:1589437/1‑148 (MQ=255)
                                                                      cgcgGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGc                                                                              <  1:2880249/148‑1 (MQ=255)
                                                                               cTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCcgc                                                                     <  2:1420783/148‑1 (MQ=255)
                                                                                  gTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGATACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTc                                                                  <  2:2664081/148‑1 (MQ=255)
                                                                                   tGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACTATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCg                                                                 >  1:89790/1‑148 (MQ=255)
                                                                                   tGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCg                                                                 <  1:1076226/148‑1 (MQ=255)
                                                                                              ttGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTaa                                                      >  2:2736259/1‑148 (MQ=255)
                                                                                              tgggCTGAAGAGAAAAAAATCCCCCAAGATGTGCGTTCACACGGGCAGAAAGATACGGGGTTTGCCTTTCCTTCCGGGCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTaa                                                      <  2:1781182/146‑1 (MQ=255)
                                                                                                ggCTGAAGAGAAAAATATCCCACAATAGTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAAcc                                                    >  2:3155984/1‑148 (MQ=255)
                                                                                                 gCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCa                                                   <  2:363092/148‑1 (MQ=255)
                                                                                                  cTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGATACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCAt                                                  <  1:2839570/148‑1 (MQ=255)
                                                                                                   tGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCAtt                                                 <  1:1418407/148‑1 (MQ=255)
                                                                                                    gAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTg                                                <  1:1203581/148‑1 (MQ=255)
                                                                                                    gAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTg                                                >  1:169296/1‑148 (MQ=255)
                                                                                                    gAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTg                                                >  1:2488520/1‑148 (MQ=255)
                                                                                                    gAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTg                                                >  1:2488614/1‑148 (MQ=255)
                                                                                                     aaGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGc                                               <  2:1197281/148‑1 (MQ=255)
                                                                                                       gagaAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTa                                             >  1:20341/1‑148 (MQ=255)
                                                                                                        agaAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTAt                                            <  1:2645567/148‑1 (MQ=255)
                                                                                                        agaAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTAt                                            >  1:2852390/1‑148 (MQ=255)
                                                                                                         gaAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATc                                           <  1:2184205/148‑1 (MQ=255)
                                                                                                             aaTATCCCACAATATTTGCGTTCACACTGGCAGAAAGATACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGc                                       >  2:1569227/1‑148 (MQ=255)
                                                                                                              atatCCCACAATATTTGCGTTCACACTGGCAGAAAGATACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCt                                      >  1:2675653/1‑148 (MQ=255)
                                                                                                               tatCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACAATTGCTATCTTGCTg                                     >  2:977648/1‑148 (MQ=255)
                                                                                                                 tCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGt                                   >  1:1703178/1‑148 (MQ=255)
                                                                                                                 tCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGt                                   >  1:1703175/1‑148 (MQ=255)
                                                                                                                 tCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGt                                   <  2:2241156/148‑1 (MQ=255)
                                                                                                                  cccACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTc                                  >  1:3123923/1‑148 (MQ=255)
                                                                                                                  cccACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTc                                  >  2:508090/1‑148 (MQ=255)
                                                                                                                   ccACAATATTTGCGTTCACACTGGCAGAAAGATACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCt                                 <  2:1504210/148‑1 (MQ=255)
                                                                                                                   ccACAATATGTGCGTTCACACTGGCAGAAAGATACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCt                                 <  2:441110/148‑1 (MQ=255)
                                                                                                                      caATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTggg                              >  1:1580382/1‑148 (MQ=255)
                                                                                                                       aaTATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGc                             >  2:959649/1‑148 (MQ=255)
                                                                                                                         tatTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCaa                           <  2:2626267/148‑1 (MQ=255)
                                                                                                                           tttGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACg                         <  2:2967376/148‑1 (MQ=255)
                                                                                                                            ttGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACgg                        >  1:2150678/1‑148 (MQ=255)
                                                                                                                              gCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGa                      >  1:1009072/1‑148 (MQ=255)
                                                                                                                               cGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTCGGCAACGGGAg                     >  2:1496098/1‑148 (MQ=255)
                                                                                                                               cGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCAGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAg                     >  2:409428/1‑148 (MQ=255)
                                                                                                                                 ttCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTc                   >  1:1392737/1‑148 (MQ=255)
                                                                                                                                     cacTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATgg               >  1:2358442/1‑148 (MQ=255)
                                                                                                                                       cTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGa             <  2:2343771/148‑1 (MQ=255)
                                                                                                                                         ggCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAg           <  1:670670/148‑1 (MQ=255)
                                                                                                                                          gCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAgc          >  2:1406050/1‑148 (MQ=255)
                                                                                                                                             gAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAgccgc       <  1:1215/148‑1 (MQ=255)
                                                                                                                                             gAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAgccgc       <  2:710272/148‑1 (MQ=255)
                                                                                                                                              aaaGAGACGGTGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAgccgcc      <  2:940543/148‑1 (MQ=255)
                                                                                                                                              aaaGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAgccgcc      <  2:1924647/148‑1 (MQ=255)
                                                                                                                                               aaGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCct     >  1:2961369/1‑148 (MQ=255)
                                                                                                                                                agagACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCctg    >  2:2835476/1‑148 (MQ=255)
                                                                                                                                                agagACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCctg    <  1:2305969/148‑1 (MQ=255)
                                                                                                                                                agagACGGGGTTTGCCTGTCCTTCCGGTCACACGATGTGTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCctg    <  2:2423757/148‑1 (MQ=255)
                                                                                                                                                 gagaCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCACCGGGAGTCATGGGAAGCCGCctgc   >  2:372535/1‑148 (MQ=255)
                                                                                                                                                 gagaCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCctgc   <  1:857616/148‑1 (MQ=255)
                                                                                                                                                 gagaCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCctgc   <  2:20343/148‑1 (MQ=255)
                                                                                                                                                 gagaCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCctgc   <  2:1526866/148‑1 (MQ=255)
                                                                                                                                                  agaCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCctgct  >  1:707002/1‑148 (MQ=255)
                                                                                                                                                  agaCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCctgct  >  2:1570766/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTTGTTATCTGGCTGGAAAAAACACATCATATTCCGGTTGATGAGTTCTACACTTTAAAGCGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCCTGCT  >  CP009273/1333901‑1334194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A