Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,407,969 C→A 85.9% N249K (AAC→AAA)  acrE → cytoplasmic membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,407,9690CA85.9% 417.8 / 77.6 192N249K (AAC→AAA) acrEcytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (15/12);  new base A (92/73);  total (107/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

Only 100 of 193 total aligned reads displayed.

AACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAA  >  CP009273/3407824‑3408116
                                                                                                                                                 |                                                                                                                                                   
aaCTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACgg                                                                                                                                                   >  1:2760753/1‑148 (MQ=255)
 acgggCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGt                                                                                                                                                  <  2:1287211/145‑1 (MQ=255)
    ggCGACTGTCCAGCAGCTCTATCCTATCTACGTTGATGTGACCCAATCCAGCAACTATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCaa                                                                                                                                               <  2:1780187/148‑1 (MQ=255)
    ggCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCaa                                                                                                                                               <  2:1779997/148‑1 (MQ=255)
     gCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCaaa                                                                                                                                              <  1:272200/148‑1 (MQ=255)
     gCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCaaa                                                                                                                                              >  2:3217360/1‑148 (MQ=255)
      cGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAAAGTAGAGTTGGTCATGGAAAAAGGTCAAAc                                                                                                                                             >  2:2186124/1‑148 (MQ=255)
      cGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGAAACGATTTTATGAGGCTGAAGAAAGCCGTAGAGCAAGTAAATTTGCATAAGTAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAAc                                                                                                                                             <  2:1121456/148‑1 (MQ=255)
        aCTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCt                                                                                                                                           >  2:1883643/1‑148 (MQ=255)
           gTCCAGCAGCTCTATCCTATATACGTTGTTGTGACCCAATCCACCAAGTATTTTATGAGGCTGAACAAATCCGTAGAGCAAGGAAATTTCCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATc                                                                                                                                        <  1:597603/148‑1 (MQ=255)
            tCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATcc                                                                                                                                       <  2:2598723/148‑1 (MQ=255)
             ccAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATccc                                                                                                                                      <  2:2989915/148‑1 (MQ=255)
              cagcagCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATcccc                                                                                                                                     <  2:2885269/148‑1 (MQ=255)
                gcagcTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTg                                                                                                                                   >  1:3302240/1‑148 (MQ=255)
                    cTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAg                                                                                                                               >  1:1928606/1‑148 (MQ=255)
                         tCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACg                                                                                                                          <  1:1341152/148‑1 (MQ=255)
                         tCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACg                                                                                                                          >  2:1521446/1‑148 (MQ=255)
                            tATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAAAGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTg                                                                                                                       <  1:2186033/148‑1 (MQ=255)
                             aTCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGc                                                                                                                      >  2:3294858/1‑148 (MQ=255)
                             aTCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGc                                                                                                                      >  1:1390435/1‑148 (MQ=255)
                             aTCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGc                                                                                                                      >  1:1494158/1‑148 (MQ=255)
                                 aCGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAAtt                                                                                                                  <  2:2389513/148‑1 (MQ=255)
                                   gTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATtct                                                                                                                <  1:398084/148‑1 (MQ=255)
                                         gtgACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAtg                                                                                                          <  1:775848/148‑1 (MQ=255)
                                         gtgACCCAATCCAGCAACGATTTTATGAGGCTGAAGAAATCCGTAGAGCAAGGAAATTTGAATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAtg                                                                                                          <  1:569308/148‑1 (MQ=255)
                                           gACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAtgtg                                                                                                        <  2:3303920/148‑1 (MQ=255)
                                           gACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAtgtg                                                                                                        <  2:3152175/148‑1 (MQ=255)
                                               cAATCCAGCAACGATTTTCTGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCg                                                                                                    <  2:1818574/148‑1 (MQ=255)
                                                agtCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGt                                                                                                   >  2:1818280/3‑148 (MQ=255)
                                                 aTCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGtt                                                                                                  <  2:2748760/148‑1 (MQ=255)
                                                  tCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAGCCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTtg                                                                                                 >  1:925295/1‑148 (MQ=255)
                                                   ccAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTtga                                                                                                <  2:2643672/148‑1 (MQ=255)
                                                    cAGCAACTATTTTTGGAGGCTGAAGCAATCCGTAGAGCAAGTAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTtgat                                                                                               <  1:1138901/148‑1 (MQ=255)
                                                       cAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGaa                                                                                            >  2:2802419/1‑148 (MQ=255)
                                                       cAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGaa                                                                                            >  2:205880/1‑148 (MQ=255)
                                                         aCGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAg                                                                                          <  2:2327404/148‑1 (MQ=255)
                                                           taTTTTATGAGTCTTAATCAATCCGTAGAGAAAGTAAATTTTAATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCa                                                                                        <  1:1750971/147‑1 (MQ=255)
                                                           gATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCa                                                                                        <  1:3154733/148‑1 (MQ=255)
                                                             ttttATGAGGCTGAAGAAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCAcc                                                                                      <  1:3125213/148‑1 (MQ=255)
                                                              tttATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCg                                                                                     >  2:1838760/1‑148 (MQ=255)
                                                                   gAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTcc                                                                                >  1:2014621/1‑148 (MQ=255)
                                                                   gAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTcc                                                                                <  2:1252872/148‑1 (MQ=255)
                                                                      gCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATa                                                                             <  2:641958/148‑1 (MQ=255)
                                                                      gCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATa                                                                             >  2:1203592/1‑148 (MQ=255)
                                                                       cTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATaa                                                                            >  1:770557/1‑148 (MQ=255)
                                                                        tGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAAc                                                                           <  2:2171944/148‑1 (MQ=255)
                                                                         gAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAAcc                                                                          <  1:3292636/148‑1 (MQ=255)
                                                                          aaGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAAccc                                                                         >  2:3252367/1‑148 (MQ=255)
                                                                          aaGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAAccc                                                                         >  2:343335/1‑148 (MQ=255)
                                                                           aGCAATCCGTAGAGCAAGTAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCt                                                                        <  1:664801/148‑1 (MQ=255)
                                                                             cAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCACTTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTAc                                                                      >  2:2905705/1‑148 (MQ=255)
                                                                                tCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTg                                                                   >  1:912007/1‑148 (MQ=255)
                                                                                tCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTg                                                                   >  2:2265506/1‑148 (MQ=255)
                                                                                  cGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCt                                                                 <  2:1952339/148‑1 (MQ=255)
                                                                                    tAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGt                                                               >  1:934980/1‑148 (MQ=255)
                                                                                    tAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGt                                                               <  1:2576105/148‑1 (MQ=255)
                                                                                    tAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGt                                                               >  1:2919145/1‑148 (MQ=255)
                                                                                      gagCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCt                                                             >  2:71822/1‑148 (MQ=255)
                                                                                      gagCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCt                                                             >  1:815935/1‑148 (MQ=255)
                                                                                      gagCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCAACGTGCTGTCt                                                             >  2:385425/1‑148 (MQ=255)
                                                                                         cAAGGAAATTTGCATAAGGAACACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTcc                                                          <  1:421668/148‑1 (MQ=255)
                                                                                          atgtaaaTTTTCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTccc                                                         <  2:3105239/144‑1 (MQ=255)
                                                                                          aaGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTccc                                                         >  2:3045695/1‑148 (MQ=255)
                                                                                          aaGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTccc                                                         <  2:1390435/148‑1 (MQ=255)
                                                                                           aGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCt                                                        >  1:2003118/1‑148 (MQ=255)
                                                                                            ggAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTa                                                       >  2:2955344/1‑148 (MQ=255)
                                                                                             gAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTaa                                                      >  2:2107192/1‑148 (MQ=255)
                                                                                               aaTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAAcc                                                    <  2:2598491/148‑1 (MQ=255)
                                                                                                aTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAAccc                                                   >  1:2495250/1‑148 (MQ=255)
                                                                                                 tttGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCg                                                  <  2:1268439/148‑1 (MQ=255)
                                                                                                 tttGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCg                                                  >  1:1993769/1‑148 (MQ=255)
                                                                                                    gCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCaa                                               <  1:3217360/148‑1 (MQ=255)
                                                                                                     cATAAGGAAAACTCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAAc                                              <  2:517644/148‑1 (MQ=255)
                                                                                                     cATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAAc                                              >  2:1125957/1‑148 (MQ=255)
                                                                                                       tAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACAt                                            >  1:1091003/1‑148 (MQ=255)
                                                                                                        aaGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATa                                           >  1:728895/1‑148 (MQ=255)
                                                                                                         aGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATAc                                          >  1:1078005/1‑148 (MQ=255)
                                                                                                         aGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATAc                                          >  1:2688938/1‑148 (MQ=255)
                                                                                                          ggAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACg                                         >  2:870135/1‑148 (MQ=255)
                                                                                                          ggAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACg                                         <  2:2944192/148‑1 (MQ=255)
                                                                                                          ggAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACg                                         <  1:1649974/148‑1 (MQ=255)
                                                                                                           gAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGc                                        >  2:871689/1‑148 (MQ=255)
                                                                                                             aaaCGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCtt                                      <  2:1353542/148‑1 (MQ=255)
                                                                                                             aaaCGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCtt                                      <  1:547101/148‑1 (MQ=255)
                                                                                                              aaCGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCttt                                     >  2:1225305/1‑148 (MQ=255)
                                                                                                               aCGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCtttt                                    >  1:2335481/1‑148 (MQ=255)
                                                                                                                        gCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTAt                           <  1:1719061/148‑1 (MQ=255)
                                                                                                                         cAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATg                          <  2:797739/148‑1 (MQ=255)
                                                                                                                          aaCGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGt                         <  2:1576582/148‑1 (MQ=255)
                                                                                                                            cGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGttt                       >  2:479318/1‑148 (MQ=255)
                                                                                                                                   ttGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTgtgcgtg                >  1:2269079/1‑148 (MQ=255)
                                                                                                                                   ttGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTgtgcgtg                >  2:2849881/1‑148 (MQ=255)
                                                                                                                                    tGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTgtgcgtgc               >  1:622137/1‑148 (MQ=255)
                                                                                                                                     ggTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCACTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCa              >  2:3188578/1‑148 (MQ=255)
                                                                                                                                       tCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACg            >  1:1298882/1‑148 (MQ=255)
                                                                                                                                        cATGGAAAAAGGTAAAACCTATCCCCTGAAATGTACGCTGCAATTCTCCGATGTGTCCGTTGTTTAAAGCACCGGCTCGATAACCCTACGTGCTGTCGTCCCTATCGCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACgg           <  2:459683/148‑1 (MQ=255)
                                                                                                                                          tGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGAt         >  1:1192361/1‑148 (MQ=255)
                                                                                                                                             aaaaCGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATtga      >  1:1434628/1‑148 (MQ=255)
                                                                                                                                             aaaaAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATtga      >  1:502123/1‑148 (MQ=255)
                                                                                                                                             aaaaAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATtga      >  1:828631/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAA  >  CP009273/3407824‑3408116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGG  >  CP009273/3407820‑3408118
                                                                                                                                                     |                                                                                                                                                     
ACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCACGCCACCAACGATTTTATGCGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAA                                                                                                                                                       <  SRR19973070.59325/150‑1 (MQ=60)
  TGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGG                                                                                                                                                     >  SRR19973070.2778454/1‑150 (MQ=60)
         GCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACC                                                                                                                                              <  SRR19973070.273980/150‑1 (MQ=60)
               GTCCAGCAGCTCTATCCTATATACGTTGTTGTGACCCAATCCACCAAGTATTTTATGAGGCTGAACAAATCCGTAGAGCAAGGAAATTTCCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCC                                                                                                                                        <  SRR19973070.601756/150‑1 (MQ=60)
               GTCCAGCAGCACGATCCTATCTACTTTTATGTGACCCAATCCAGCAACTTTTTTATGAGGCTTAAGAAATCCTTAGAGCATGTAAATTTGCATAAGTAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCC                                                                                                                                        <  SRR19973070.601539/150‑1 (MQ=60)
                  CAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTG                                                                                                                                     >  SRR19973070.3323385/1‑150 (MQ=60)
                   AGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGA                                                                                                                                    >  SRR19973070.1786857/1‑150 (MQ=60)
                      AGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAG                                                                                                                                 <  SRR19973070.1111010/150‑1 (MQ=60)
                      AGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAG                                                                                                                                 >  SRR19973070.1941601/1‑150 (MQ=60)
                       GCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGG                                                                                                                                >  SRR19973070.1362799/1‑150 (MQ=60)
                         TCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTA                                                                                                                              <  SRR19973070.2722698/150‑1 (MQ=60)
                             TCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCT                                                                                                                          <  SRR19973070.1350366/150‑1 (MQ=60)
                             TCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCT                                                                                                                          <  SRR19973070.1630653/150‑1 (MQ=60)
                               CTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGC                                                                                                                        >  SRR19973070.1504614/1‑150 (MQ=60)
                               CTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGC                                                                                                                        >  SRR19973070.1399825/1‑150 (MQ=60)
                                TATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAAAGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCA                                                                                                                       <  SRR19973070.2200574/150‑1 (MQ=60)
                                TATCTACGTTGATGTGACCAAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAAAGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCA                                                                                                                       <  SRR19973070.2200483/150‑1 (MQ=60)
                                 ATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAA                                                                                                                      >  SRR19973070.534668/1‑150 (MQ=60)
                                       GTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCC                                                                                                                >  SRR19973070.1420754/1‑150 (MQ=60)
                                       GTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCC                                                                                                                <  SRR19973070.401013/150‑1 (MQ=60)
                                       GTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCC                                                                                                                <  SRR19973070.470229/150‑1 (MQ=60)
                                             GTGACCCAATCCCGCAAATATTTTATGAGGCTGAAGCAATCCTTAGAGCAAGGAAATTTGCATAAGCAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTG                                                                                                          <  SRR19973070.573069/150‑1 (MQ=60)
                                             GTGACCCAATCCAGCAACGTTTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTG                                                                                                          <  SRR19973070.781262/150‑1 (MQ=60)
                                             GTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTG                                                                                                          <  SRR19973070.781279/150‑1 (MQ=60)
                                             GTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTG                                                                                                          <  SRR19973070.378192/150‑1 (MQ=60)
                                             GTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGAAATCCGTAGAGCAAGGAAATTTGAATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTG                                                                                                          <  SRR19973070.573154/150‑1 (MQ=60)
                                              TGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGA                                                                                                         >  SRR19973070.2405486/1‑150 (MQ=60)
                                                    AATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAGCCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTG                                                                                                   >  SRR19973070.931890/1‑150 (MQ=60)
                                                        CAGCAACTATTTTTGGAGGCTGAAGCAATCCGTAGAGCAAGTAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGA                                                                                               <  SRR19973070.1146796/150‑1 (MQ=60)
                                                            AACTATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGTAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGC                                                                                           <  SRR19973070.2530386/150‑1 (MQ=60)
                                                               TATTTTATGAGTCTTAATCAATCCGTAGAGAAAGTAAATTTTAATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACC                                                                                        <  SRR19973070.1763104/150‑1 (MQ=60)
                                                               GATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACC                                                                                        >  SRR19973070.1989434/1‑150 (MQ=60)
                                                               GATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACC                                                                                        >  SRR19973070.3207670/1‑150 (MQ=60)
                                                               GATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACC                                                                                        <  SRR19973070.3175120/150‑1 (MQ=60)
                                                                 TTTTATGAGGCTGAAGAAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGG                                                                                      <  SRR19973070.3145390/150‑1 (MQ=60)
                                                                     ATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCC                                                                                  >  SRR19973070.2028248/1‑150 (MQ=60)
                                                                         GGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAA                                                                              >  SRR19973070.775961/1‑150 (MQ=60)
                                                                             GAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCT                                                                          <  SRR19973070.3313754/150‑1 (MQ=60)
                                                                              AAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTA                                                                         <  SRR19973070.797652/150‑1 (MQ=60)
                                                                               AGCAATCCGTAGAGCAAGTAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTAC                                                                        <  SRR19973070.669544/150‑1 (MQ=60)
                                                                               AGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTAC                                                                        <  SRR19973070.2685810/150‑1 (MQ=60)
                                                                                GCAATCCGTAGAGAAAGTAAATTTGAATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACG                                                                       <  SRR19973070.415230/150‑1 (MQ=60)
                                                                                 CAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGT                                                                      >  SRR19973070.1177175/1‑150 (MQ=60)
                                                                                 CAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGT                                                                      >  SRR19973070.2179298/1‑150 (MQ=60)
                                                                                  AATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTG                                                                     >  SRR19973070.918497/1‑150 (MQ=60)
                                                                                      CGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGT                                                                 >  SRR19973070.941649/1‑150 (MQ=60)
                                                                                      CGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGT                                                                 >  SRR19973070.3155737/1‑150 (MQ=60)
                                                                                      CGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGT                                                                 >  SRR19973070.2937996/1‑150 (MQ=60)
                                                                                        TAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCT                                                               <  SRR19973070.2592945/150‑1 (MQ=60)
                                                                                        TAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCT                                                               >  SRR19973070.2016441/1‑150 (MQ=60)
                                                                                        TAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCT                                                               >  SRR19973070.821515/1‑150 (MQ=60)
                                                                                             CAAGGAAATTTGCATAAGGAACACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCT                                                          <  SRR19973070.424742/150‑1 (MQ=60)
                                                                                             CAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCT                                                          >  SRR19973070.2764212/1‑150 (MQ=60)
                                                                                             CAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCT                                                          >  SRR19973070.1726418/1‑150 (MQ=60)
                                                                                             CAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCT                                                          >  SRR19973070.2016678/1‑150 (MQ=60)
                                                                                                  AAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCC                                                     >  SRR19973070.2511432/1‑150 (MQ=60)
                                                                                                   AATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCG                                                    >  SRR19973070.3005242/1‑150 (MQ=60)
                                                                                                   AATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCG                                                    >  SRR19973070.2007270/1‑150 (MQ=60)
                                                                                                        GCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACA                                               <  SRR19973070.3238162/150‑1 (MQ=60)
                                                                                                         CATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACAT                                              >  SRR19973070.1098542/1‑150 (MQ=60)
                                                                                                         CATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACAT                                              >  SRR19973070.738086/1‑150 (MQ=60)
                                                                                                          ATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATA                                             >  SRR19973070.734063/1‑150 (MQ=60)
                                                                                                           TAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATAC                                            >  SRR19973070.1085508/1‑150 (MQ=60)
                                                                                                           TAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATAC                                            >  SRR19973070.2706361/1‑150 (MQ=60)
                                                                                                              GGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCT                                         <  SRR19973070.1661366/150‑1 (MQ=60)
                                                                                                                 AAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTT                                      <  SRR19973070.550751/150‑1 (MQ=60)
                                                                                                                 AAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTT                                      >  SRR19973070.679376/1‑150 (MQ=60)
                                                                                                                 AAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTT                                      >  SRR19973070.2350980/1‑150 (MQ=60)
                                                                                                                       CTCCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGG                                <  SRR19973070.923964/150‑1 (MQ=60)
                                                                                                                        ACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGT                               >  SRR19973070.3266593/1‑150 (MQ=60)
                                                                                                                            GCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGT                           <  SRR19973070.1730948/150‑1 (MQ=60)
                                                                                                                             CAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTT                          <  SRR19973070.653668/150‑1 (MQ=60)
                                                                                                                                  TAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGC                     <  SRR19973070.2302379/150‑1 (MQ=60)
                                                                                                                                     AGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTG                  >  SRR19973070.2284143/1‑150 (MQ=60)
                                                                                                                                     AGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTG                  >  SRR19973070.2889238/1‑150 (MQ=60)
                                                                                                                                      GTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGC                 >  SRR19973070.626522/1‑150 (MQ=60)
                                                                                                                                      GTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGC                 >  SRR19973070.626523/1‑150 (MQ=60)
                                                                                                                                         GGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACG              >  SRR19973070.1307922/1‑150 (MQ=60)
                                                                                                                                         GGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACG              >  SRR19973070.1307978/1‑150 (MQ=60)
                                                                                                                                            CATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGAT           >  SRR19973070.1731313/1‑150 (MQ=60)
                                                                                                                                            CATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGAT           >  SRR19973070.1200745/1‑150 (MQ=60)
                                                                                                                                            CATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGAT           >  SRR19973070.1276150/1‑150 (MQ=60)
                                                                                                                                               GGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTCTGTTTGTGCGTGCACGGATTGA        >  SRR19973070.1447369/1‑150 (MQ=60)
                                                                                                                                               GGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGA        >  SRR19973070.1444555/1‑150 (MQ=60)
                                                                                                                                               GGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGA        >  SRR19973070.505608/1‑150 (MQ=60)
                                                                                                                                               GGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGA        >  SRR19973070.834002/1‑150 (MQ=60)
                                                                                                                                               GGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGA        >  SRR19973070.834260/1‑150 (MQ=60)
                                                                                                                                               GGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGA        <  SRR19973070.939579/150‑1 (MQ=60)
                                                                                                                                                  AAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGA     >  SRR19973070.1825844/1‑150 (MQ=60)
                                                                                                                                                    AAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAG   <  SRR19973070.1314636/150‑1 (MQ=60)
                                                                                                                                                    AAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAG   >  SRR19973070.1150961/1‑150 (MQ=60)
                                                                                                                                                    AAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAG   >  SRR19973070.1150912/1‑150 (MQ=60)
                                                                                                                                                    AAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAG   <  SRR19973070.2974364/150‑1 (MQ=60)
                                                                                                                                                     AGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGG  <  SRR19973070.2875507/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGG  >  CP009273/3407820‑3408118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑