Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,173,145 A→T 89.5% Q658L (CAG→CTG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,173,1450AT89.5% 595.7 / 50.2 200Q658L (CAG→CTG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (8/13);  new base T (90/89);  total (98/102)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 203 total aligned reads displayed.

GAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACG  >  CP009273/4173000‑4173289
                                                                                                                                                 |                                                                                                                                                
   gaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAgg                                                                                                                                             >  1:1883052/1‑148 (MQ=255)
    aagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAggt                                                                                                                                            >  2:3250233/1‑148 (MQ=255)
    aagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAggt                                                                                                                                            >  2:56330/1‑148 (MQ=255)
     agGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAggtg                                                                                                                                           <  1:3215857/148‑1 (MQ=255)
      ggCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAggtgg                                                                                                                                          <  1:335808/148‑1 (MQ=255)
           cTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATcc                                                                                                                                     <  1:1013562/148‑1 (MQ=255)
             aCGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGt                                                                                                                                   <  1:1788419/148‑1 (MQ=255)
               gTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCg                                                                                                                                 <  1:3080038/148‑1 (MQ=255)
               gTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCg                                                                                                                                 <  2:46617/148‑1 (MQ=255)
                ttATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCgg                                                                                                                                <  1:933661/148‑1 (MQ=255)
                ttATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCgg                                                                                                                                <  1:1337217/148‑1 (MQ=255)
                 tATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGt                                                                                                                               >  1:1996394/1‑148 (MQ=255)
                     gCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGt                                                                                                                           <  1:2543244/148‑1 (MQ=255)
                       ccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTcc                                                                                                                         <  2:742899/148‑1 (MQ=255)
                       ccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTcc                                                                                                                         <  2:31957/148‑1 (MQ=255)
                         aGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCt                                                                                                                       >  1:1501136/1‑148 (MQ=255)
                         aGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCt                                                                                                                       <  1:2121594/148‑1 (MQ=255)
                         aGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCt                                                                                                                       >  2:1294263/1‑148 (MQ=255)
                          ggCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTg                                                                                                                      >  2:1603495/1‑148 (MQ=255)
                          ggCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTg                                                                                                                      >  2:1603138/1‑148 (MQ=255)
                              aaCTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATcc                                                                                                                  >  2:246366/1‑148 (MQ=255)
                                  ccAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCttt                                                                                                              <  2:478320/148‑1 (MQ=255)
                                   cAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTc                                                                                                             <  2:1582671/148‑1 (MQ=255)
                                   cAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTc                                                                                                             <  2:2165727/148‑1 (MQ=255)
                                   cAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTc                                                                                                             <  2:2679902/148‑1 (MQ=255)
                                     aCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCt                                                                                                           >  2:2000760/1‑148 (MQ=255)
                                       ttGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTgg                                                                                                         >  2:2179420/1‑148 (MQ=255)
                                           aTGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCCGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAaca                                                                                                     >  2:1339806/1‑148 (MQ=255)
                                           aTGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAaca                                                                                                     >  1:2496572/1‑148 (MQ=255)
                                            tGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAacac                                                                                                    >  2:1947181/1‑148 (MQ=255)
                                             gaagaaGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACg                                                                                                   >  2:1952661/1‑148 (MQ=255)
                                             gaagaaGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACg                                                                                                   <  2:1730807/148‑1 (MQ=255)
                                             gaagaaGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACg                                                                                                   <  2:1646663/148‑1 (MQ=255)
                                             gaagaaGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACg                                                                                                   <  2:2901951/148‑1 (MQ=255)
                                               agaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGAt                                                                                                 >  1:2615991/1‑148 (MQ=255)
                                                gaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATg                                                                                                <  1:63859/148‑1 (MQ=255)
                                                gaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATg                                                                                                <  1:64082/148‑1 (MQ=255)
                                                gaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATg                                                                                                <  2:262097/148‑1 (MQ=255)
                                                   ggCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACg                                                                                             <  1:975363/148‑1 (MQ=255)
                                                       aCTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCaa                                                                                         <  2:552236/148‑1 (MQ=255)
                                                       aCTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCaa                                                                                         <  2:553262/148‑1 (MQ=255)
                                                         ttCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAAcc                                                                                       >  2:867411/1‑148 (MQ=255)
                                                             tAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGc                                                                                   >  1:1835188/1‑148 (MQ=255)
                                                                aagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCAtt                                                                                >  1:2438028/1‑148 (MQ=255)
                                                                 agaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTg                                                                               >  1:422942/1‑148 (MQ=255)
                                                                 agaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTg                                                                               >  1:423450/1‑148 (MQ=255)
                                                                 agaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTg                                                                               >  2:2423050/1‑148 (MQ=255)
                                                                 agaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTg                                                                               >  1:2179861/1‑148 (MQ=255)
                                                                  gaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGa                                                                              <  2:1480270/148‑1 (MQ=255)
                                                                    ccTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATg                                                                            <  2:1240613/148‑1 (MQ=255)
                                                                    ccTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATg                                                                            >  2:355711/1‑148 (MQ=255)
                                                                    ccTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATg                                                                            >  2:634960/1‑148 (MQ=255)
                                                                      tGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATggg                                                                          <  1:2778397/148‑1 (MQ=255)
                                                                         tAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGc                                                                       <  2:243624/148‑1 (MQ=255)
                                                                          aaCTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCg                                                                      >  1:2066019/1‑148 (MQ=255)
                                                                           aCTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGa                                                                     >  1:3181874/1‑148 (MQ=255)
                                                                            cTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGaa                                                                    >  2:1252669/1‑148 (MQ=255)
                                                                             ttGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAAc                                                                   <  1:3019999/148‑1 (MQ=255)
                                                                              tGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACa                                                                  <  2:2096180/148‑1 (MQ=255)
                                                                              tGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTGTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACa                                                                  <  2:430973/148‑1 (MQ=255)
                                                                               gCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAt                                                                 <  2:2725138/148‑1 (MQ=255)
                                                                               gCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAt                                                                 >  1:2908512/1‑148 (MQ=255)
                                                                                  gTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCa                                                              <  1:810283/148‑1 (MQ=255)
                                                                                   tAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCaa                                                             <  1:631165/148‑1 (MQ=255)
                                                                                   tAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCaa                                                             <  1:461630/148‑1 (MQ=255)
                                                                                      cAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCACCCGTGCATTGATGGGTGCGAACATGCAACGt                                                          >  2:2292216/1‑148 (MQ=255)
                                                                                      cAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGt                                                          >  1:4428/1‑148 (MQ=255)
                                                                                       aaaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTc                                                         >  1:1369119/1‑148 (MQ=255)
                                                                                       aaaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTc                                                         <  1:3296793/148‑1 (MQ=255)
                                                                                          ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAgg                                                      >  1:1621564/1‑148 (MQ=255)
                                                                                          ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAgg                                                      >  1:22046/1‑148 (MQ=255)
                                                                                          ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAgg                                                      >  2:1527763/1‑148 (MQ=255)
                                                                                                    gCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAc                                            >  2:1070890/1‑148 (MQ=255)
                                                                                                     cTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAct                                           <  1:1131838/148‑1 (MQ=255)
                                                                                                     cTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAct                                           <  2:448676/148‑1 (MQ=255)
                                                                                                     cTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAct                                           <  1:1231162/148‑1 (MQ=255)
                                                                                                     cTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAct                                           <  1:1231176/148‑1 (MQ=255)
                                                                                                      ttGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGActc                                          <  2:260001/148‑1 (MQ=255)
                                                                                                          tCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcg                                      <  1:1657674/148‑1 (MQ=255)
                                                                                                             gCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgcgc                                   >  2:1829535/1‑148 (MQ=255)
                                                                                                                 cgACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGAt                               >  1:1321464/1‑148 (MQ=255)
                                                                                                                   aCCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCTGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATaa                             >  1:2174603/1‑148 (MQ=255)
                                                                                                                   aCCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATaa                             <  2:2229921/148‑1 (MQ=255)
                                                                                                                   aCCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATaa                             >  1:2986395/1‑148 (MQ=255)
                                                                                                                     cAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGc                           <  2:1698587/148‑1 (MQ=255)
                                                                                                                       ggTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCg                         <  1:407376/148‑1 (MQ=255)
                                                                                                                           gACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtgg                     <  1:2987716/148‑1 (MQ=255)
                                                                                                                           gACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtgg                     <  2:537290/148‑1 (MQ=255)
                                                                                                                           gACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtgg                     <  2:3027993/148‑1 (MQ=255)
                                                                                                                            aCTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt                    <  1:578820/148‑1 (MQ=255)
                                                                                                                               aCATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttgg                 <  2:1539219/148‑1 (MQ=255)
                                                                                                                                cATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttggt                >  1:2141292/1‑148 (MQ=255)
                                                                                                                                  tGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc              >  1:3158467/1‑148 (MQ=255)
                                                                                                                                    gACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTg            >  1:1421507/1‑148 (MQ=255)
                                                                                                                                    gACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTg            >  2:2585273/1‑148 (MQ=255)
                                                                                                                                      cGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGt          <  2:2066019/148‑1 (MQ=255)
                                                                                                                                       gTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTa         <  1:1511420/148‑1 (MQ=255)
                                                                                                                                          tCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATgg      >  2:2989042/1‑148 (MQ=255)
                                                                                                                                           ccACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGa     <  2:2123552/148‑1 (MQ=255)
                                                                                                                                              cccTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACg  <  2:1915480/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACG  >  CP009273/4173000‑4173289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGC  >  CP009273/4172997‑4173292
                                                                                                                                                    |                                                                                                                                                   
ATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTG                                                                                                                                                    <  SRR19973070.1563605/150‑1 (MQ=60)
ATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTG                                                                                                                                                    >  SRR19973070.1489981/1‑150 (MQ=60)
   GAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAG                                                                                                                                                 >  SRR19973070.3130033/1‑150 (MQ=60)
    AAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGG                                                                                                                                                >  SRR19973070.1895849/1‑150 (MQ=60)
       AAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGG                                                                                                                                             <  SRR19973070.96419/150‑1 (MQ=60)
        AGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGT                                                                                                                                            <  SRR19973070.3236652/150‑1 (MQ=60)
         GGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTA                                                                                                                                           <  SRR19973070.338255/150‑1 (MQ=60)
          GCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTAT                                                                                                                                          <  SRR19973070.1251103/150‑1 (MQ=60)
              CTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGT                                                                                                                                      <  SRR19973070.1020762/150‑1 (MQ=60)
              CTACGTTATCGCCCAGGCGAACTCCAACTTGGAAGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGAATCCACCCTGCAGGTGGTATCCGT                                                                                                                                      >  SRR19973070.1653619/1‑150 (MQ=60)
                ACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCG                                                                                                                                    <  SRR19973070.1800781/150‑1 (MQ=60)
                ACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCG                                                                                                                                    <  SRR19973070.1853121/150‑1 (MQ=60)
                  GTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGT                                                                                                                                  >  SRR19973070.2012626/1‑150 (MQ=60)
                  GTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGT                                                                                                                                  >  SRR19973070.2009922/1‑150 (MQ=60)
                  GTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGT                                                                                                                                  <  SRR19973070.3099828/150‑1 (MQ=60)
                   TTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTG                                                                                                                                 <  SRR19973070.1346416/150‑1 (MQ=60)
                   TTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTG                                                                                                                                 <  SRR19973070.1346309/150‑1 (MQ=60)
                   TTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTG                                                                                                                                 <  SRR19973070.940323/150‑1 (MQ=60)
                        GCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCC                                                                                                                            <  SRR19973070.2559874/150‑1 (MQ=60)
                          CCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCT                                                                                                                          >  SRR19973070.1511647/1‑150 (MQ=60)
                          CCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCT                                                                                                                          >  SRR19973070.1511650/1‑150 (MQ=60)
                            AGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGA                                                                                                                        <  SRR19973070.2135799/150‑1 (MQ=60)
                                      CAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCT                                                                                                              >  SRR19973070.2085191/1‑150 (MQ=60)
                                      CAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCT                                                                                                              >  SRR19973070.1002189/1‑150 (MQ=60)
                                       AACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTG                                                                                                             <  SRR19973070.1410243/150‑1 (MQ=60)
                                            GGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACA                                                                                                        >  SRR19973070.2512764/1‑150 (MQ=60)
                                            GGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACA                                                                                                        >  SRR19973070.252708/1‑150 (MQ=60)
                                             GATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACAC                                                                                                       >  SRR19973070.1588398/1‑150 (MQ=60)
                                                GAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGAT                                                                                                    >  SRR19973070.2633057/1‑150 (MQ=60)
                                                  AGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGA                                                                                                  >  SRR19973070.2940524/1‑150 (MQ=60)
                                                   GAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGAC                                                                                                 <  SRR19973070.64570/150‑1 (MQ=60)
                                                   GAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGAC                                                                                                 <  SRR19973070.64739/150‑1 (MQ=60)
                                                   GAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGAC                                                                                                 <  SRR19973070.64801/150‑1 (MQ=60)
                                                      GGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCC                                                                                              <  SRR19973070.982320/150‑1 (MQ=60)
                                                       GCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCA                                                                                             <  SRR19973070.2199665/150‑1 (MQ=60)
                                                       GCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCA                                                                                             <  SRR19973070.2193846/150‑1 (MQ=60)
                                                              CGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGC                                                                                      >  SRR19973070.1847799/1‑150 (MQ=60)
                                                                 AGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATT                                                                                   >  SRR19973070.2454009/1‑150 (MQ=60)
                                                                  GAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTG                                                                                  <  SRR19973070.857932/150‑1 (MQ=60)
                                                                  GAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTG                                                                                  >  SRR19973070.426535/1‑150 (MQ=60)
                                                                  GAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTG                                                                                  >  SRR19973070.426523/1‑150 (MQ=60)
                                                                  GAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTG                                                                                  >  SRR19973070.426024/1‑150 (MQ=60)
                                                                  GAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTG                                                                                  >  SRR19973070.1028993/1‑150 (MQ=60)
                                                                  GAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTG                                                                                  >  SRR19973070.3060627/1‑150 (MQ=60)
                                                                  GAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTG                                                                                  >  SRR19973070.2194289/1‑150 (MQ=60)
                                                                         TGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTG                                                                           <  SRR19973070.2796249/150‑1 (MQ=60)
                                                                           GTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCG                                                                         >  SRR19973070.2079950/1‑150 (MQ=60)
                                                                            TAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGA                                                                        >  SRR19973070.450271/1‑150 (MQ=60)
                                                                            TAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGA                                                                        >  SRR19973070.3202502/1‑150 (MQ=60)
                                                                            TAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGA                                                                        >  SRR19973070.3202464/1‑150 (MQ=60)
                                                                            TAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGA                                                                        >  SRR19973070.2736457/1‑150 (MQ=60)
                                                                             AACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAA                                                                       >  SRR19973070.60661/1‑150 (MQ=60)
                                                                               CTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACA                                                                     <  SRR19973070.2876560/150‑1 (MQ=60)
                                                                                TTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAT                                                                    <  SRR19973070.3039249/150‑1 (MQ=60)
                                                                                TTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAT                                                                    <  SRR19973070.3039214/150‑1 (MQ=60)
                                                                                TTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAT                                                                    >  SRR19973070.2927322/1‑150 (MQ=60)
                                                                                TTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAT                                                                    <  SRR19973070.3300399/150‑1 (MQ=60)
                                                                                    CGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAA                                                                <  SRR19973070.1949587/150‑1 (MQ=60)
                                                                                     GTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAAC                                                               <  SRR19973070.2484836/150‑1 (MQ=60)
                                                                                     GTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAAC                                                               <  SRR19973070.815834/150‑1 (MQ=60)
                                                                                      TAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACG                                                              <  SRR19973070.635722/150‑1 (MQ=60)
                                                                                      TAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACG                                                              <  SRR19973070.635638/150‑1 (MQ=60)
                                                                                      TAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACG                                                              <  SRR19973070.464923/150‑1 (MQ=60)
                                                                                       AGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGT                                                             >  SRR19973070.4499/1‑150 (MQ=60)
                                                                                        GCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTC                                                            >  SRR19973070.1378435/1‑150 (MQ=60)
                                                                                        GCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATACACCCTGCAGGTGGTCTCAGTCGGTGCGTCCCTGATCCCTTTCCTCGAACACGATGACGCCAACCGTGCATTGATGGGTGCGACCATGCAACGTC                                                            >  SRR19973070.1378424/1‑150 (MQ=60)
                                                                                          AAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAG                                                          >  SRR19973070.1202755/1‑150 (MQ=60)
                                                                                          AAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAG                                                          <  SRR19973070.3317923/150‑1 (MQ=60)
                                                                                          AAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAG                                                          <  SRR19973070.336404/150‑1 (MQ=60)
                                                                                           AAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGG                                                         >  SRR19973070.709132/1‑150 (MQ=60)
                                                                                           AAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGG                                                         >  SRR19973070.1632837/1‑150 (MQ=60)
                                                                                           AAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGG                                                         >  SRR19973070.22367/1‑150 (MQ=60)
                                                                                             GGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCC                                                       <  SRR19973070.440439/150‑1 (MQ=60)
                                                                                                   TCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCG                                                 <  SRR19973070.471815/150‑1 (MQ=60)
                                                                                                        CTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCT                                            <  SRR19973070.1139656/150‑1 (MQ=60)
                                                                                                        CTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCT                                            <  SRR19973070.1239793/150‑1 (MQ=60)
                                                                                                        CTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCT                                            <  SRR19973070.1239788/150‑1 (MQ=60)
                                                                                                        CTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCT                                            <  SRR19973070.1239802/150‑1 (MQ=60)
                                                                                                             TCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCG                                       <  SRR19973070.1669096/150‑1 (MQ=60)
                                                                                                                  CGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGAT                                  >  SRR19973070.1330603/1‑150 (MQ=60)
                                                                                                                    CGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCTGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAA                                >  SRR19973070.2189013/1‑150 (MQ=60)
                                                                                                                    CGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAA                                >  SRR19973070.3005540/1‑150 (MQ=60)
                                                                                                                    CGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAA                                >  SRR19973070.2189003/1‑150 (MQ=60)
                                                                                                                          GGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCT                          <  SRR19973070.411436/150‑1 (MQ=60)
                                                                                                                          GGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCT                          <  SRR19973070.410356/150‑1 (MQ=60)
                                                                                                                          GGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCT                          <  SRR19973070.410361/150‑1 (MQ=60)
                                                                                                                              GACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTT                      <  SRR19973070.3006863/150‑1 (MQ=60)
                                                                                                                              GACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTT                      <  SRR19973070.3006609/150‑1 (MQ=60)
                                                                                                                               ACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTG                     >  SRR19973070.1678683/1‑150 (MQ=60)
                                                                                                                               ACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTG                     <  SRR19973070.582801/150‑1 (MQ=60)
                                                                                                                                CTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGG                    >  SRR19973070.3207822/1‑150 (MQ=60)
                                                                                                                                 TACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGT                   >  SRR19973070.2155567/1‑150 (MQ=60)
                                                                                                                                   CATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAC                 >  SRR19973070.3178906/1‑150 (MQ=60)
                                                                                                                                     TGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCCTTGATGGGTGCGAACATGCAACGGCAGGCCGTTCCGACTCTGCGCGCTGAGAAGCCGCTGGTTGGGACTG               >  SRR19973070.2139813/1‑150 (MQ=60)
                                                                                                                                     TGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTG               >  SRR19973070.1431846/1‑150 (MQ=60)
                                                                                                                                     TGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTG               >  SRR19973070.2579501/1‑150 (MQ=60)
                                                                                                                                     TGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTG               >  SRR19973070.1431270/1‑150 (MQ=60)
                                                                                                                                     TGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTG               >  SRR19973070.2412828/1‑150 (MQ=60)
                                                                                                                                         CGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTAT           >  SRR19973070.3198351/1‑150 (MQ=60)
                                                                                                                                          GTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATG          <  SRR19973070.1521990/150‑1 (MQ=60)
                                                                                                                                          GTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATG          >  SRR19973070.1806085/1‑150 (MQ=60)
                                                                                                                                                  CCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCTTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGC  >  SRR19973070.541285/1‑150 (MQ=60)
                                                                                                                                                  CCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGC  >  SRR19973070.1102504/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
ATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGC  >  CP009273/4172997‑4173292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑