Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 232,860 G→T 100% D103Y (GAT→TAT)  dnaQ → DNA polymerase III epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273232,8600GT99.5% 712.0 / ‑4.1 216D103Y (GAT→TAT) dnaQDNA polymerase III epsilon subunit
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (87/128);  total (87/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 217 total aligned reads displayed.

CGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCT  >  CP009273/232715‑233003
                                                                                                                                                 |                                                                                                                                               
cGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTat                                                                                                                                               >  2:2292535/1‑148 (MQ=255)
cGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTat                                                                                                                                               <  2:629538/148‑1 (MQ=255)
 gATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCtata                                                                                                                                              <  2:2189706/148‑1 (MQ=255)
   tCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATc                                                                                                                                            >  1:2288532/1‑148 (MQ=255)
   tCGGCGGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATc                                                                                                                                            <  1:1325551/148‑1 (MQ=255)
     ggCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCgg                                                                                                                                          <  2:2346117/148‑1 (MQ=255)
      gCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGc                                                                                                                                         >  1:231139/1‑148 (MQ=255)
      gCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGc                                                                                                                                         >  1:2262727/1‑148 (MQ=255)
        tggtggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCtt                                                                                                                                       <  2:484036/148‑1 (MQ=255)
        tggtggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCtt                                                                                                                                       <  2:2272199/148‑1 (MQ=255)
         ggtggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCttt                                                                                                                                      >  1:2513487/1‑148 (MQ=255)
          gtggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTa                                                                                                                                     <  2:2837478/148‑1 (MQ=255)
             gATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATgg                                                                                                                                  <  1:486573/148‑1 (MQ=255)
              aTCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGa                                                                                                                                 <  1:3167112/148‑1 (MQ=255)
              aTCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGa                                                                                                                                 <  1:3167379/148‑1 (MQ=255)
              aTCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGa                                                                                                                                 <  2:38630/148‑1 (MQ=255)
                   gAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAATCCCACCTATGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGTCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGTACTACg                                                                                                                            >  1:3015841/1‑148 (MQ=255)
                    aaGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGa                                                                                                                           >  2:704431/1‑148 (MQ=255)
                      gCCTTTGGCGTACATGGTTTTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGt                                                                                                                         <  2:3435436/148‑1 (MQ=255)
                      gCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGt                                                                                                                         <  1:1547138/148‑1 (MQ=255)
                       ccTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGtt                                                                                                                        <  1:2184451/148‑1 (MQ=255)
                         tttGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGtttt                                                                                                                      <  1:1680342/148‑1 (MQ=255)
                         tttGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGtttt                                                                                                                      <  2:2095884/148‑1 (MQ=255)
                         tttGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTATATATCGGCTTTATGGACTACGAGtttt                                                                                                                      <  1:2578529/148‑1 (MQ=255)
                                 aCATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCtt                                                                                                              <  1:297244/148‑1 (MQ=255)
                                   aTGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTaa                                                                                                            <  2:340051/148‑1 (MQ=255)
                                    tGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAg                                                                                                           >  1:3061324/1‑148 (MQ=255)
                                       tATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                                                                                                        <  1:2654921/148‑1 (MQ=255)
                                        aTTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGcgcg                                                                                                       <  1:709112/148‑1 (MQ=255)
                                        aTTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGcgcg                                                                                                       >  1:90927/1‑148 (MQ=255)
                                        aTTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGcgcg                                                                                                       <  1:1932369/148‑1 (MQ=255)
                                          tGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGat                                                                                                     <  1:2616412/148‑1 (MQ=255)
                                          tGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTACCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTAAATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAATCTCGat                                                                                                     >  1:742749/1‑148 (MQ=255)
                                           gCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGata                                                                                                    <  2:1398943/148‑1 (MQ=255)
                                            ccGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGatat                                                                                                   >  1:344239/1‑148 (MQ=255)
                                               aTGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTcc                                                                                                >  2:594872/1‑148 (MQ=255)
                                                tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCg                                                                                               >  1:294073/1‑148 (MQ=255)
                                                tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCg                                                                                               >  1:438735/1‑148 (MQ=255)
                                                 gAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa                                                                                              >  1:3085004/1‑148 (MQ=255)
                                                 gAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa                                                                                              <  2:675368/148‑1 (MQ=255)
                                                 gAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa                                                                                              <  2:2458484/148‑1 (MQ=255)
                                                 gAATTTTTGCTCGATAAGCACACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATCTTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTGGCTTAAGCGCGATATTCCGa                                                                                              <  2:1855873/148‑1 (MQ=255)
                                                  aaTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGaa                                                                                             <  1:3003694/148‑1 (MQ=255)
                                                   aTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAg                                                                                            >  1:644312/1‑148 (MQ=255)
                                                    tttttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGa                                                                                           >  2:156106/1‑148 (MQ=255)
                                                       ttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCa                                                                                        <  2:798128/148‑1 (MQ=255)
                                                        tGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCaa                                                                                       <  2:1585634/148‑1 (MQ=255)
                                                         gCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAAt                                                                                      <  1:1036629/148‑1 (MQ=255)
                                                            cGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACt                                                                                   <  1:2462653/148‑1 (MQ=255)
                                                            cGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACt                                                                                   <  2:1662613/148‑1 (MQ=255)
                                                            cGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACt                                                                                   <  2:3421949/148‑1 (MQ=255)
                                                            cGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACt                                                                                   <  2:1475611/148‑1 (MQ=255)
                                                             gATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACtt                                                                                  <  2:2091848/148‑1 (MQ=255)
                                                             gATAAGCCCACGTGTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACtt                                                                                  <  2:3372540/148‑1 (MQ=255)
                                                              aTAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACttt                                                                                 <  2:2173083/148‑1 (MQ=255)
                                                               tAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTc                                                                                <  2:2940826/148‑1 (MQ=255)
                                                                 aGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTg                                                                              <  1:437651/148‑1 (MQ=255)
                                                                 aGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTg                                                                              <  1:2226531/148‑1 (MQ=255)
                                                                  gCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGt                                                                             <  2:351734/148‑1 (MQ=255)
                                                                  gCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGt                                                                             <  1:2103134/148‑1 (MQ=255)
                                                                   cccACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTa                                                                            <  2:3294584/148‑1 (MQ=255)
                                                                    ccACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTaa                                                                           <  2:2020123/148‑1 (MQ=255)
                                                                       cGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGt                                                                        <  2:527433/148‑1 (MQ=255)
                                                                          ttGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCAc                                                                     >  2:1227765/1‑148 (MQ=255)
                                                                          ttGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCAc                                                                     <  1:1106398/148‑1 (MQ=255)
                                                                               gAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATa                                                                >  1:1957282/1‑148 (MQ=255)
                                                                                aaGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAg                                                               >  1:1804046/1‑148 (MQ=255)
                                                                                  gTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGcc                                                             >  2:2611785/1‑148 (MQ=255)
                                                                                    aGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCtt                                                           <  1:1065923/148‑1 (MQ=255)
                                                                                         aTGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGt                                                      >  2:1161926/1‑148 (MQ=255)
                                                                                         aTGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGt                                                      >  1:116684/1‑148 (MQ=255)
                                                                                         aTGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGt                                                      >  1:116727/1‑148 (MQ=255)
                                                                                         aTGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGt                                                      >  2:1301702/1‑148 (MQ=255)
                                                                                           gAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTgg                                                    >  1:2627515/1‑148 (MQ=255)
                                                                                             gTTCATGGACTATCTTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCg                                                  <  2:2956716/148‑1 (MQ=255)
                                                                                              ttCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGa                                                 <  2:2236593/148‑1 (MQ=255)
                                                                                              ttCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGa                                                 <  2:2807152/148‑1 (MQ=255)
                                                                                               tCATGGACTATAGTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAg                                                <  1:3487633/148‑1 (MQ=255)
                                                                                                        atatTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGtt                                       >  2:2991348/1‑148 (MQ=255)
                                                                                                         tatTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGttt                                      <  1:83543/148‑1 (MQ=255)
                                                                                                           ttCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTcc                                    <  2:3371007/148‑1 (MQ=255)
                                                                                                            tCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTccc                                   >  2:288566/1‑148 (MQ=255)
                                                                                                                ggcgcggAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTa                               <  1:803290/148‑1 (MQ=255)
                                                                                                                        gTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAAc                       <  1:516923/148‑1 (MQ=255)
                                                                                                                         ttGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACa                      <  2:1804046/148‑1 (MQ=255)
                                                                                                                         ttGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACa                      >  2:1931974/1‑148 (MQ=255)
                                                                                                                          tGGTGATCCATAACGCAGCGTTCTGTATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAg                     <  2:221742/148‑1 (MQ=255)
                                                                                                                          tGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAg                     >  1:3301430/1‑148 (MQ=255)
                                                                                                                           ggTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGc                    >  2:2497732/1‑148 (MQ=255)
                                                                                                                            gTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGcc                   <  2:2108736/148‑1 (MQ=255)
                                                                                                                             tGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCACTACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCt                  >  2:1189720/1‑148 (MQ=255)
                                                                                                                             tGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCt                  >  2:1189689/1‑148 (MQ=255)
                                                                                                                                tCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGa               <  1:1820215/148‑1 (MQ=255)
                                                                                                                                        gCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTAt       <  2:2373446/148‑1 (MQ=255)
                                                                                                                                         cAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTAtg      <  1:584763/148‑1 (MQ=255)
                                                                                                                                         cAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTAtg      <  2:2405374/148‑1 (MQ=255)
                                                                                                                                           gCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTAtgtg    <  1:2839215/148‑1 (MQ=255)
                                                                                                                                            cGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGc   >  2:3022984/1‑148 (MQ=255)
                                                                                                                                            cGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGc   >  1:250229/1‑148 (MQ=255)
                                                                                                                                             gTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTCAGCGCGATATTCCGAAGACCTATACTTTCTGTATGGTCACCGATAGCCGTGCGGTGGCGAGGTAAATGTTTCCCGGTTAGCGCAACAGCCTCGATGCGTTATGTGCt  <  2:2845050/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCT  >  CP009273/232715‑233003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCG  >  CP009273/232712‑233005
                                                                                                                                                    |                                                                                                                                                 
ACCCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTA                                                                                                                                                  <  SRR19973067.1653271/150‑1 (MQ=60)
    GATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATC                                                                                                                                              >  SRR19973067.2303657/1‑150 (MQ=60)
      TCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGG                                                                                                                                            <  SRR19973067.2541977/150‑1 (MQ=60)
      TCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGG                                                                                                                                            <  SRR19973067.747126/150‑1 (MQ=60)
      TCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGG                                                                                                                                            <  SRR19973067.2611069/150‑1 (MQ=60)
      TCGGCGGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGG                                                                                                                                            <  SRR19973067.1334598/150‑1 (MQ=60)
       CGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGC                                                                                                                                           >  SRR19973067.2277735/1‑150 (MQ=60)
       CGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGC                                                                                                                                           >  SRR19973067.232789/1‑150 (MQ=60)
          CTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTT                                                                                                                                        >  SRR19973067.2529972/1‑150 (MQ=60)
             GTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATG                                                                                                                                     >  SRR19973067.2829533/1‑150 (MQ=60)
             GTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATG                                                                                                                                     <  SRR19973067.1961680/150‑1 (MQ=60)
                GATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGAC                                                                                                                                  <  SRR19973067.3470384/150‑1 (MQ=60)
                GATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGAC                                                                                                                                  <  SRR19973067.489923/150‑1 (MQ=60)
                 ATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACT                                                                                                                                 <  SRR19973067.3187395/150‑1 (MQ=60)
                 ATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACT                                                                                                                                 <  SRR19973067.3187165/150‑1 (MQ=60)
                 ATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACT                                                                                                                                 <  SRR19973067.3187432/150‑1 (MQ=60)
                    CGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAATCCCACCTATGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGTCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGTACTACG                                                                                                                              >  SRR19973067.3035033/1‑150 (MQ=60)
                    CGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACG                                                                                                                              <  SRR19973067.1765187/150‑1 (MQ=60)
                    CGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACG                                                                                                                              >  SRR19973067.3034866/1‑150 (MQ=60)
                     GGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGA                                                                                                                             >  SRR19973067.806550/1‑150 (MQ=60)
                      GAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAG                                                                                                                            <  SRR19973067.2186409/150‑1 (MQ=60)
                      GAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAG                                                                                                                            <  SRR19973067.2210340/150‑1 (MQ=60)
                       AAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGT                                                                                                                           >  SRR19973067.1997929/1‑150 (MQ=60)
                         GCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTT                                                                                                                         <  SRR19973067.1557659/150‑1 (MQ=60)
                          CCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTT                                                                                                                        <  SRR19973067.1566658/150‑1 (MQ=60)
                          CCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTT                                                                                                                        <  SRR19973067.2199051/150‑1 (MQ=60)
                           CTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTC                                                                                                                       <  SRR19973067.3104901/150‑1 (MQ=60)
                            TTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCG                                                                                                                      <  SRR19973067.1691922/150‑1 (MQ=60)
                            TTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCG                                                                                                                      <  SRR19973067.3507747/150‑1 (MQ=60)
                            TTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTATATATCGGCTTTATGGACTACGAGTTTTCG                                                                                                                      <  SRR19973067.2595330/150‑1 (MQ=60)
                                 CGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCT                                                                                                                 <  SRR19973067.1546765/150‑1 (MQ=60)
                                    ACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAA                                                                                                              <  SRR19973067.299138/150‑1 (MQ=60)
                                     CATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAG                                                                                                             >  SRR19973067.3080813/1‑150 (MQ=60)
                                         GTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCG                                                                                                         >  SRR19973067.91894/1‑150 (MQ=60)
                                         GTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCG                                                                                                         >  SRR19973067.2283968/1‑150 (MQ=60)
                                          TATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGA                                                                                                        >  SRR19973067.309876/1‑150 (MQ=60)
                                          TATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGA                                                                                                        <  SRR19973067.2672022/150‑1 (MQ=60)
                                           ATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGAT                                                                                                       <  SRR19973067.2107968/150‑1 (MQ=60)
                                           ATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGAT                                                                                                       <  SRR19973067.714024/150‑1 (MQ=60)
                                           ATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGAT                                                                                                       <  SRR19973067.1945550/150‑1 (MQ=60)
                                           ATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTACCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTAAATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAATCTCGAT                                                                                                       >  SRR19973067.747894/1‑150 (MQ=60)
                                            TTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATA                                                                                                      <  SRR19973067.2638835/150‑1 (MQ=60)
                                             TGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAT                                                                                                     <  SRR19973067.2633385/150‑1 (MQ=60)
                                             TGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAT                                                                                                     <  SRR19973067.836757/150‑1 (MQ=60)
                                             TGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAT                                                                                                     >  SRR19973067.346616/1‑150 (MQ=60)
                                                CGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCC                                                                                                  >  SRR19973067.246461/1‑150 (MQ=60)
                                                 GATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCG                                                                                                 >  SRR19973067.441788/1‑150 (MQ=60)
                                                 GATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCG                                                                                                 >  SRR19973067.441779/1‑150 (MQ=60)
                                                 GATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCG                                                                                                 >  SRR19973067.296141/1‑150 (MQ=60)
                                                 GATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCG                                                                                                 >  SRR19973067.295957/1‑150 (MQ=60)
                                                  ATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGA                                                                                                <  SRR19973067.3317883/150‑1 (MQ=60)
                                                  ATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGA                                                                                                >  SRR19973067.3104634/1‑150 (MQ=60)
                                                    GAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAG                                                                                              >  SRR19973067.648657/1‑150 (MQ=60)
                                                    GAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAG                                                                                              >  SRR19973067.648664/1‑150 (MQ=60)
                                                     AATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGA                                                                                             <  SRR19973067.3022766/150‑1 (MQ=60)
                                                       TTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACC                                                                                           <  SRR19973067.2946773/150‑1 (MQ=60)
                                                        TTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCA                                                                                          <  SRR19973067.109884/150‑1 (MQ=60)
                                                           TGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATA                                                                                       >  SRR19973067.2437529/1‑150 (MQ=60)
                                                           TGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATA                                                                                       >  SRR19973067.1122034/1‑150 (MQ=60)
                                                           TGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATA                                                                                       <  SRR19973067.562787/150‑1 (MQ=60)
                                                            GCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATAC                                                                                      <  SRR19973067.1043761/150‑1 (MQ=60)
                                                               CGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTT                                                                                   <  SRR19973067.2478839/150‑1 (MQ=60)
                                                                 ATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCT                                                                                 >  SRR19973067.3042409/1‑150 (MQ=60)
                                                                 ATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCT                                                                                 <  SRR19973067.1496713/150‑1 (MQ=60)
                                                                    AGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTA                                                                              <  SRR19973067.2658141/150‑1 (MQ=60)
                                                                    AGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTA                                                                              <  SRR19973067.440697/150‑1 (MQ=60)
                                                                    AGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTA                                                                              <  SRR19973067.2658190/150‑1 (MQ=60)
                                                                    AGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTA                                                                              <  SRR19973067.2241352/150‑1 (MQ=60)
                                                                     GCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAA                                                                             <  SRR19973067.2117087/150‑1 (MQ=60)
                                                                      CCCACGTTTGCCGAAGTAGCCGTTGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAG                                                                            <  SRR19973067.3464034/150‑1 (MQ=60)
                                                                      CCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAG                                                                            <  SRR19973067.303789/150‑1 (MQ=60)
                                                                      CCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAG                                                                            <  SRR19973067.3569203/150‑1 (MQ=60)
                                                                           GTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCAC                                                                       <  SRR19973067.3007360/150‑1 (MQ=60)
                                                                           GTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCAC                                                                       >  SRR19973067.187043/1‑150 (MQ=60)
                                                                             TTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCG                                                                     <  SRR19973067.1113912/150‑1 (MQ=60)
                                                                                CCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATA                                                                  >  SRR19973067.1970593/1‑150 (MQ=60)
                                                                                 CGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGNTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAG                                                                 >  SRR19973067.1816194/1‑150 (MQ=60)
                                                                                 CGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAG                                                                 >  SRR19973067.668784/1‑150 (MQ=60)
                                                                                    AGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCT                                                              <  SRR19973067.2519597/150‑1 (MQ=60)
                                                                                     GTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTT                                                             <  SRR19973067.2503391/150‑1 (MQ=60)
                                                                                       AGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGC                                                           <  SRR19973067.1073233/150‑1 (MQ=60)
                                                                                          CGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGT                                                        >  SRR19973067.117799/1‑150 (MQ=60)
                                                                                          CGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGT                                                        >  SRR19973067.117827/1‑150 (MQ=60)
                                                                                          CGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGT                                                        >  SRR19973067.117842/1‑150 (MQ=60)
                                                                                          CGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGT                                                        >  SRR19973067.118020/1‑150 (MQ=60)
                                                                                            ATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGG                                                      >  SRR19973067.2644543/1‑150 (MQ=60)
                                                                                                  TCATGGACTATAGTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGA                                                <  SRR19973067.3509766/150‑1 (MQ=60)
                                                                                                            TATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCC                                      <  SRR19973067.84455/150‑1 (MQ=60)
                                                                                                             ATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCC                                     >  SRR19973067.1052126/1‑150 (MQ=60)
                                                                                                                   GGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAG                               <  SRR19973067.808815/150‑1 (MQ=60)
                                                                                                                           GTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAG                       <  SRR19973067.520426/150‑1 (MQ=60)
                                                                                                                           GTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAG                       >  SRR19973067.22982/1‑150 (MQ=60)
                                                                                                                           GTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAG                       >  SRR19973067.3322231/1‑150 (MQ=60)
                                                                                                                              GGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCT                    >  SRR19973067.848587/1‑150 (MQ=60)
                                                                                                                                   TCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATG               <  SRR19973067.1832551/150‑1 (MQ=60)
                                                                                                                                    CCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGC              <  SRR19973067.630890/150‑1 (MQ=60)
                                                                                                                                            CAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTG      <  SRR19973067.588504/150‑1 (MQ=60)
                                                                                                                                             AGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCAGCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGC     >  SRR19973067.1820003/1‑150 (MQ=60)
                                                                                                                                             AGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGC     >  SRR19973067.475828/1‑150 (MQ=60)
                                                                                                                                             AGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGC     >  SRR19973067.251959/1‑150 (MQ=60)
                                                                                                                                              GCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCT    <  SRR19973067.2857446/150‑1 (MQ=60)
                                                                                                                                                GTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCG  <  SRR19973067.508829/150‑1 (MQ=60)
                                                                                                                                                GTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCG  <  SRR19973067.2307677/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                 
ACCCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCG  >  CP009273/232712‑233005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑