Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 361,673 A→T 21.9% V24E (GTG→GAG)  lacI ← DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273361,6730AT21.9% 16619.5 / inf 7604V24E (GTG→GAG) lacIDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (2834/3100);  new base T (764/899);  total (3600/4004)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-01

Only 100 of 7925 total aligned reads displayed.

AGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGT  >  CP009273/361526‑361820
                                                                                                                                                   |                                                                                                                                                    
  ggCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTcacc                                                                                                                                                    >  2:1253628/1‑148 (MQ=255)
  ggCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTcacc                                                                                                                                                    <  2:800339/148‑1 (MQ=255)
  ggCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTcacc                                                                                                                                                    <  1:1397952/148‑1 (MQ=255)
     cAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCACGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCAcg                                                                                                                                                 <  1:2288427/148‑1 (MQ=255)
       gACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCTcgcg                                                                                                                                               >  1:256009/1‑148 (MQ=255)
        aCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCgg                                                                                                                                              >  1:3020913/1‑148 (MQ=255)
        aCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTACAATTTTTCCCGCGTTTTCGCAGAAACGTTGCTGGCCTGGTTCACCAAGCgg                                                                                                                                              >  1:2495779/1‑148 (MQ=255)
           ggAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑aa                                                                                                                                          <  2:3374303/148‑1 (MQ=255)
            gAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑aaa                                                                                                                                         >  1:392329/1‑148 (MQ=255)
             aGGTGGCAACGCCAATCAGCAACGACTGTGTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAAc                                                                                                                                        <  1:99383/148‑1 (MQ=255)
               gTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTCATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACgg                                                                                                                                      <  1:1707119/148‑1 (MQ=255)
                tGGCAACGCCAATCAGCAACGACTGTTTGCGCGCCTGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTGCGCCATCGCCGCTTCCACATTTTCCCGCGTTTTCGCAGTAACGTGGCTGGCCTGGTTCACCACGCGGT‑AAACGGt                                                                                                                                     <  2:2257785/148‑1 (MQ=255)
                tGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGt                                                                                                                                     <  1:1000194/148‑1 (MQ=255)
                tGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGt                                                                                                                                     <  2:2454102/148‑1 (MQ=255)
                 ggCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTc                                                                                                                                    <  1:1245986/148‑1 (MQ=255)
                  gCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCt                                                                                                                                   >  2:1728569/1‑148 (MQ=255)
                  gCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCt                                                                                                                                   >  2:2902470/1‑148 (MQ=255)
                  gCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGGGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCt                                                                                                                                   >  1:1583883/1‑148 (MQ=255)
                   cAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTg                                                                                                                                  >  1:3414691/1‑148 (MQ=255)
                    aaCGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAAACGGTCTg                                                                                                                                  <  1:1655474/148‑1 (MQ=255)
                     aCGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGAt                                                                                                                                >  2:2211049/1‑148 (MQ=255)
                      cGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTtata                                                                                                                               >  1:1919133/1‑148 (MQ=255)
                      cGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATa                                                                                                                               <  2:3238133/148‑1 (MQ=255)
                        ccAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAag                                                                                                                             <  2:1589452/148‑1 (MQ=255)
                           aTCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAgaga                                                                                                                          <  2:2100520/148‑1 (MQ=255)
                           aTCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAgaga                                                                                                                          <  1:2112766/148‑1 (MQ=255)
                                cAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCg                                                                                                                     >  1:2693433/1‑148 (MQ=255)
                                cAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCg                                                                                                                     >  1:235382/1‑148 (MQ=255)
                                  aCGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGc                                                                                                                   <  2:479365/148‑1 (MQ=255)
                                   cGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCa                                                                                                                  >  2:2844875/1‑148 (MQ=255)
                                   cGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCa                                                                                                                  >  1:744919/1‑148 (MQ=255)
                                      cTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCGGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATAc                                                                                                               >  1:1775059/1‑148 (MQ=255)
                                         tttGCCCGCCAGTTGTTGTGCCACGCAGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATActct                                                                                                            <  2:1504898/148‑1 (MQ=255)
                                              ccGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGAc                                                                                                       >  2:1055253/1‑148 (MQ=255)
                                                   aGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCTCTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGt                                                                                                  >  2:3159336/1‑148 (MQ=255)
                                                   aGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGt                                                                                                  <  2:2781693/148‑1 (MQ=255)
                                                    gttgttGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGt                                                                                                  <  1:2826601/148‑1 (MQ=255)
                                                     ttgttgTGCCACGCGGTTGGGAATGTTATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGtat                                                                                                <  2:874451/148‑1 (MQ=255)
                                                      tgttgtGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACCCCGGCATACTCTGCGACATCGtata                                                                                               >  1:3207032/1‑148 (MQ=255)
                                                      tgttgtGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGtata                                                                                               >  2:2551824/1‑148 (MQ=255)
                                                       gttgtGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATaa                                                                                              <  1:920759/148‑1 (MQ=255)
                                                         tgtgCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACg                                                                                            <  1:2426330/148‑1 (MQ=255)
                                                          gtgCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGt                                                                                           <  1:3482464/148‑1 (MQ=255)
                                                            gCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTa                                                                                         <  2:136709/148‑1 (MQ=255)
                                                             ccACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTAc                                                                                        <  2:2040216/148‑1 (MQ=255)
                                                               aCGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTg                                                                                      <  2:13179/148‑1 (MQ=255)
                                                               aCGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTg                                                                                      <  1:2180927/148‑1 (MQ=255)
                                                                cgcgGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTgg                                                                                     <  2:2157887/148‑1 (MQ=255)
                                                                 gcgGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGt                                                                                    <  2:1792908/148‑1 (MQ=255)
                                                                 gcgGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGt                                                                                    <  1:52569/148‑1 (MQ=255)
                                                                 gcgGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCGGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGt                                                                                    <  2:2496407/148‑1 (MQ=255)
                                                                     ttGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTca                                                                                <  2:3042609/148‑1 (MQ=255)
                                                                       gggAATGTAATTCAGCTCCGCCATCGCCGCTTGCACTTTTTCCCGCGTTTTAGCAGAATCGTGGCTGGCCTGGTTCTCCACGCGGG‑AAAGTGTCTGATAAGAGACACCGGCATACTCTGCGTCATCGTATTACGTTTCTGGTTTcaca                                                                              <  2:1988298/148‑1 (MQ=255)
                                                                       gggAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTcaca                                                                              <  2:9387/148‑1 (MQ=255)
                                                                         gAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTACCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGGATAACGTTACTGGTTTCACAtt                                                                            >  1:2962606/1‑148 (MQ=255)
                                                                            tGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTcac                                                                         >  1:871116/1‑148 (MQ=255)
                                                                              tAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATGAGAGACACCGGCAAACTCTGCGACATCGTATAACGTTACTGGTTTCACATTcacca                                                                       >  1:1113457/1‑148 (MQ=255)
                                                                                aTTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCaccacc                                                                     <  2:185783/148‑1 (MQ=255)
                                                                                 ttCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCAccc                                                                    <  2:2325034/148‑1 (MQ=255)
                                                                                 ttCAGCTCCGCCATCGCCGCTTACACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑TAACGGTCTTATAAGAGACACCGGCATACTCTTCGACATCGTATAACGTTACTGGTTTAACAATCACCAccc                                                                    >  1:1320197/1‑148 (MQ=255)
                                                                                    aGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAAAGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGa                                                                 >  2:1183657/1‑148 (MQ=255)
                                                                                      cTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAAt                                                               >  2:1656246/1‑148 (MQ=255)
                                                                                       tCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAAAGTTACTGGTTTCACATTCACCACCCTGAAtt                                                              >  2:3312429/1‑148 (MQ=255)
                                                                                        ccGCCATCGCCGCTTCCACTTTTTTCCGCGTTTTCGCAGAAAAGTGGCTGGGCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGACACCCCGGCATACGCTGCGACATCGTATAACGTTACTGGTTTCACATTAACCACCCTGAATTg                                                             >  1:676970/1‑148 (MQ=255)
                                                                                         cGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGa                                                            <  1:1054041/148‑1 (MQ=255)
                                                                                          gCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGATACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGAc                                                           >  1:2759171/1‑148 (MQ=255)
                                                                                           ccATCGCCGCTTCCACGTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACAGCGTATAACGTTACTGGTTTCACATTCACCACCCGGAAttttct                                                          >  1:3081698/1‑144 (MQ=255)
                                                                                             aTCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AACCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActct                                                        >  2:2605503/1‑148 (MQ=255)
                                                                                             aTCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActct                                                        <  2:730374/148‑1 (MQ=255)
                                                                                               cgccgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActctct                                                      >  2:2661291/1‑148 (MQ=255)
                                                                                               cgccgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActctct                                                      <  1:1521155/148‑1 (MQ=255)
                                                                                               cgccgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActctct                                                      <  2:1439238/148‑1 (MQ=255)
                                                                                               cgccgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActctct                                                      >  2:2975143/1‑148 (MQ=255)
                                                                                                gccgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCTCGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCtt                                                     <  1:3527682/148‑1 (MQ=255)
                                                                                                 ccgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTc                                                    >  1:1980150/1‑148 (MQ=255)
                                                                                                  cgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTcc                                                   >  2:536273/1‑148 (MQ=255)
                                                                                                   gcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTTGTTCACCACGCGGG‑AAAAGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCg                                                  >  1:2626200/1‑148 (MQ=255)
                                                                                                       ccACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGgcg                                              >  2:2405209/1‑148 (MQ=255)
                                                                                                       ccACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGgcg                                              >  1:3342233/1‑148 (MQ=255)
                                                                                                          cTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTa                                           <  1:1172414/148‑1 (MQ=255)
                                                                                                          cTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCGCCCTGAATTGACTCTCTTCCGGGCGCTa                                           >  1:1458034/1‑148 (MQ=255)
                                                                                                           tttttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTAt                                          <  1:460402/148‑1 (MQ=255)
                                                                                                            ttttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATc                                         >  2:357977/1‑148 (MQ=255)
                                                                                                            ttttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATc                                         <  2:424503/148‑1 (MQ=255)
                                                                                                                   gcgTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCCTGCCAt                                  >  2:1122106/1‑148 (MQ=255)
                                                                                                                   gcgTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCAt                                  <  1:3270757/148‑1 (MQ=255)
                                                                                                                    cgTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATa                                 <  2:1924287/148‑1 (MQ=255)
                                                                                                                     gTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATAc                                <  1:614768/148‑1 (MQ=255)
                                                                                                                            cAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTATCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGaaa                         >  1:1851272/1‑148 (MQ=255)
                                                                                                                             aGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTATCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAg                        <  2:2714981/148‑1 (MQ=255)
                                                                                                                               aaaCGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGt                      >  1:812076/1‑148 (MQ=255)
                                                                                                                               aaaCGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGt                      >  2:3451934/1‑148 (MQ=255)
                                                                                                                               aaaCGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGt                      >  1:2226327/1‑148 (MQ=255)
                                                                                                                                aaCGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGtt                     <  2:3502495/148‑1 (MQ=255)
                                                                                                                                      gctggcCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTgcac               >  2:66284/1‑146 (MQ=255)
                                                                                                                                        tggcCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCa             <  2:3102397/148‑1 (MQ=255)
                                                                                                                                        tggcCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCa             <  1:3146118/148‑1 (MQ=255)
                                                                                                                                             tGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGa        >  2:597712/1‑148 (MQ=255)
                                                                                                                                                ttCACCTCGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATgg     <  1:2900428/148‑1 (MQ=255)
                                                                                                                                                 tCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGTACCATTCGATGgt    <  1:2054825/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGT  >  CP009273/361526‑361820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A