Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 761,253 C→A 9.9% D27Y (GAT→TAT)  mngR ← Transcriptional repressor for the mannosyl‑D‑glycerate catabolic operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273761,2530CA 9.9% 738.2 / 36.9 211D27Y (GAT→TAT) mngRTranscriptional repressor for the mannosyl‑D‑glycerate catabolic operon
Reads supporting (aligned to +/- strand):  ref base C (89/101);  new base A (9/12);  total (98/113)
Fisher's exact test for biased strand distribution p-value = 8.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 216 total aligned reads displayed.

AATTGACCCGCTCTTCTTTGACGTAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATA  >  CP009273/761106‑761400
                                                                                                                                                   |                                                                                                                                                   
aaTTGACCCGCTCTTCTTTGACGTAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATc                                                                                                                                                     >  1:904221/1‑148 (MQ=255)
    gACCCGCTCTTCTTTGACGTAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCa                                                                                                                                                 <  1:1469146/148‑1 (MQ=255)
      cccGCTCTTCTTTGACGTAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAgg                                                                                                                                               >  1:2667423/1‑148 (MQ=255)
      cccGCTCTTCTTTGACGTAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAgg                                                                                                                                               >  2:3000060/1‑148 (MQ=255)
       ccGCTCTTCTTTGACGTAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGt                                                                                                                                              >  2:46702/1‑148 (MQ=255)
            cttcttTGACGTAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAg                                                                                                                                         >  1:2904716/1‑148 (MQ=255)
             ttcttTGACGTAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGc                                                                                                                                        <  2:1042882/148‑1 (MQ=255)
              tcttTGACGTAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCt                                                                                                                                       >  1:1127565/1‑148 (MQ=255)
                 ttGACGTAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGACTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATAGCCAGGTTTAAGCTCGc                                                                                                                                    >  2:574123/1‑148 (MQ=255)
                   gACGTAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCa                                                                                                                                  <  1:3260182/148‑1 (MQ=255)
                   gACGTAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCa                                                                                                                                  <  1:2526788/148‑1 (MQ=255)
                       tAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCAcgcg                                                                                                                              >  2:3185023/1‑148 (MQ=255)
                       tAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCAcgcg                                                                                                                              >  1:614505/1‑148 (MQ=255)
                        aaGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACgcgc                                                                                                                             <  2:2065576/148‑1 (MQ=255)
                           tCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCaag                                                                                                                          >  1:1691658/1‑147 (MQ=255)
                           tCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAAt                                                                                                                          >  1:414807/1‑148 (MQ=255)
                           tCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAAt                                                                                                                          >  1:415119/1‑148 (MQ=255)
                            cccGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAAtt                                                                                                                         >  2:1429470/1‑148 (MQ=255)
                              cGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTg                                                                                                                       <  2:2974423/148‑1 (MQ=255)
                                   cccTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCAc                                                                                                                  >  2:341535/1‑148 (MQ=255)
                                         aTGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGc                                                                                                            >  1:197457/1‑148 (MQ=255)
                                         aTGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGc                                                                                                            <  1:2943891/148‑1 (MQ=255)
                                          tGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATAGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCg                                                                                                           >  1:2978082/1‑148 (MQ=255)
                                           gCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGa                                                                                                          >  2:2878547/1‑148 (MQ=255)
                                            cTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGAt                                                                                                         <  1:2182800/148‑1 (MQ=255)
                                              ttCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCg                                                                                                       >  1:1501288/1‑148 (MQ=255)
                                               tCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCgg                                                                                                      <  2:107845/148‑1 (MQ=255)
                                                    gATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATc                                                                                                 <  1:1652236/148‑1 (MQ=255)
                                                      tCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTg                                                                                               <  1:2609320/148‑1 (MQ=255)
                                                      tCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTg                                                                                               >  2:2685286/1‑148 (MQ=255)
                                                      tCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTg                                                                                               >  1:2861110/1‑148 (MQ=255)
                                                       ctgctgCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGAGTCAGTGGGTAACGCATAGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGc                                                                                              <  1:3289227/148‑1 (MQ=255)
                                                        tgctgcTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATAGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGcc                                                                                             >  1:356893/1‑148 (MQ=255)
                                                                gACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAg                                                                                     <  1:2780162/148‑1 (MQ=255)
                                                                   tAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGc                                                                                  <  1:2705011/148‑1 (MQ=255)
                                                                   tAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGc                                                                                  <  1:930586/148‑1 (MQ=255)
                                                                   tAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGc                                                                                  <  2:189634/148‑1 (MQ=255)
                                                                   tAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATAGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGc                                                                                  >  2:3022207/1‑148 (MQ=255)
                                                                     gCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCtt                                                                                >  1:1556035/1‑148 (MQ=255)
                                                                       tGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTtgt                                                                              <  2:1573043/148‑1 (MQ=255)
                                                                       tGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTtgt                                                                              <  1:1062448/148‑1 (MQ=255)
                                                                       tGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTtgt                                                                              <  1:1316081/148‑1 (MQ=255)
                                                                              aaGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCAt                                                                       >  2:1804813/1‑148 (MQ=255)
                                                                              aaGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCAt                                                                       >  1:1372242/1‑148 (MQ=255)
                                                                              aaGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCAt                                                                       <  1:2697627/148‑1 (MQ=255)
                                                                              aaGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCAt                                                                       >  1:1264111/1‑148 (MQ=255)
                                                                               aGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCAtt                                                                      >  1:2130985/1‑148 (MQ=255)
                                                                                  cTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCAttttt                                                                   <  1:3132865/148‑1 (MQ=255)
                                                                                   tGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTa                                                                  >  2:3363074/1‑148 (MQ=255)
                                                                                   tGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTa                                                                  >  2:1444637/1‑148 (MQ=255)
                                                                                   tGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTa                                                                  <  2:3257696/148‑1 (MQ=255)
                                                                                    gACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAg                                                                 >  1:2733864/1‑148 (MQ=255)
                                                                                     aCGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGt                                                                >  1:1936933/1‑148 (MQ=255)
                                                                                       gCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTAt                                                              <  2:2582826/148‑1 (MQ=255)
                                                                                       gCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTAt                                                              <  2:502526/148‑1 (MQ=255)
                                                                                          ccGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTAtctc                                                           <  2:1225072/148‑1 (MQ=255)
                                                                                           cGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCa                                                          <  1:46702/148‑1 (MQ=255)
                                                                                             tAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCAtt                                                        >  1:31193/1‑148 (MQ=255)
                                                                                              aaCCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTa                                                       <  1:2462631/148‑1 (MQ=255)
                                                                                               aCCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTaa                                                      <  2:2967502/148‑1 (MQ=255)
                                                                                                cccGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAAt                                                     <  2:2875597/148‑1 (MQ=255)
                                                                                                cccGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAAt                                                     >  1:2767552/1‑148 (MQ=255)
                                                                                                 ccGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATa                                                    <  2:3069845/148‑1 (MQ=255)
                                                                                                  cGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATAGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATAc                                                   >  1:2600101/1‑148 (MQ=255)
                                                                                                    gCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGa                                                 <  2:1842917/148‑1 (MQ=255)
                                                                                                    gCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGa                                                 <  2:3229716/148‑1 (MQ=255)
                                                                                                      tGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAAt                                               >  2:140015/1‑148 (MQ=255)
                                                                                                      tGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATAGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAAt                                               <  1:1309890/148‑1 (MQ=255)
                                                                                                       gACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATAGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAAtt                                              <  2:831216/148‑1 (MQ=255)
                                                                                                        aCGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAAttt                                             >  2:3164502/1‑148 (MQ=255)
                                                                                                           ccAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAAc                                          <  1:593278/148‑1 (MQ=255)
                                                                                                               aCTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCAtt                                      <  1:182318/148‑1 (MQ=255)
                                                                                                                ctctGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTa                                     >  2:96010/1‑148 (MQ=255)
                                                                                                                 tctGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTATCCATTAt                                    >  2:2701724/1‑148 (MQ=255)
                                                                                                                  ctGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATg                                   <  2:2485774/148‑1 (MQ=255)
                                                                                                                            cgcTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAAt                         >  2:332530/1‑148 (MQ=255)
                                                                                                                                 aTTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATc                    <  2:1980275/148‑1 (MQ=255)
                                                                                                                                 aTTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATc                    >  1:1225731/1‑148 (MQ=255)
                                                                                                                                 aTTCAGTGGGTAACGCATAGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATc                    <  1:334335/148‑1 (MQ=255)
                                                                                                                                  ttCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATcc                   >  2:928363/1‑148 (MQ=255)
                                                                                                                                       tGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTaa              <  1:3414636/148‑1 (MQ=255)
                                                                                                                                        gggTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATTCTGTaaa             <  1:1678275/148‑1 (MQ=255)
                                                                                                                                        gggTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTaaa             <  1:1074599/148‑1 (MQ=255)
                                                                                                                                         ggTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAaat            >  2:1647641/1‑148 (MQ=255)
                                                                                                                                          gTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAaata           <  2:180681/148‑1 (MQ=255)
                                                                                                                                           tAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAaataa          >  2:3130872/1‑148 (MQ=255)
                                                                                                                                           tAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAaataa          <  2:2305493/148‑1 (MQ=255)
                                                                                                                                           tAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAaataa          <  2:2879915/148‑1 (MQ=255)
                                                                                                                                            aaCGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAaataat         <  2:1556035/148‑1 (MQ=255)
                                                                                                                                            aaCGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAaataat         <  1:1748962/148‑1 (MQ=255)
                                                                                                                                            aaCGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAaataat         <  1:3162870/148‑1 (MQ=255)
                                                                                                                                             aCGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAataata        <  2:2169387/148‑1 (MQ=255)
                                                                                                                                             aCGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAataata        <  1:90161/148‑1 (MQ=255)
                                                                                                                                             aCGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAataata        <  2:65861/148‑1 (MQ=255)
                                                                                                                                              cGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATaatac       <  1:680077/148‑1 (MQ=255)
                                                                                                                                                cATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATaatacaa     >  1:3235380/1‑148 (MQ=255)
                                                                                                                                                  tCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATaatacaaat   <  1:1213635/148‑1 (MQ=255)
                                                                                                                                                  tCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATaatacaaat   >  2:119312/1‑148 (MQ=255)
                                                                                                                                                   cGCCAGGTTTAAGCTCGCCACGCGCTATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATaatacaaata  <  2:3404961/148‑1 (MQ=255)
                                                                                                                                                   cGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATaatacaaata  >  2:1868247/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AATTGACCCGCTCTTCTTTGACGTAAGTCCCGCTGCCCTGAATGCTTTCGAGGATCTGCTGCTCGACTAGCTGGCGCAAAGCCTGACGCACCGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATA  >  CP009273/761106‑761400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A